SSGCID: Target Page

MyleA.18263.a

PROBABLE MANNOSE-6-PHOSPHATE ISOMERASE MANA (PHOSPHOMANNOSE ISOMERASE) (PHOSPHOMANNOISOMERASE) (PMI) (PHOSPHOHEXOISOMERASE) (PHO

CENTER ID:	MyleA.18263.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18263.a.A1.GE34175	full length		1	410

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1379
RefSeq:	YP_002503225.1
UniProt:	A0A0H3MPV6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MELLRGALRT YAWGSRTAIA EFTGRPVPAA HPEAELWFGA HPGDSVWLQA ANGETSLLEA LVADPEGQLG SVSRARFGDV LPFLVKVLAA DEPLSLQAHP STKQAVEGYL REERLGIPVS SPERNYRDTS HKPELLVALQ SFEALAGFRQ SSRTAELLTA LAVSDLDPFI HLLSDQSDAD GLRALFTTWI TAPQTDIDML VPAVLEGAIA YVSSGATEFL AEVKTVMELG ERYPGDAGVL ASLLLNRISL TPGEAIFLPA GNLHAYLRGV GVEVMANSDN VLRGGLTPKH VDVPELLRVL DFNPTPEEAL RPPTRCEGFA VVYETPTDEF EVALLALDGD YLGHEVDASP HDVSQGPQIL LCTEGEITVH GKFQALTVER GMAVWMAADD GPIRLVAQQP AKLFRATVGL

NT Sequence

gtggaactgc tacgcggagc gttgcggaca tatgcgtggg gatcgcggac cgctatcgcc gaattcactg gaagacctgt gccggctgct caccctgaag ctgagctctg gttcggtgcg caccccggtg actcggtttg gctacaagca gcgaacggcg aaacttcgct actggaggct ctggtcgctg atccggaagg gcagcttggc tcagtgtccc gcgctcgatt tggtgacgtg ctgccgttcc tggtcaaggt gctggcagct gacgaaccgc tgtcgctgca ggcccatccg agcaccaagc aggcggtcga gggttacctg cgcgaagaac gtctgggcat tccggtgtcc tcgccggaac gtaactaccg ggataccagt cacaagccgg agttattggt ggcgctgcag tcattcgagg cgttggctgg atttcgtcag tcgtcacgca ctgctgagct actgacggcg ttagccgtct ccgacctcga ccctttcatt catttgctga gcgaccagtc cgacgccgac ggtctgcgtg cgctgttcac tacctggata accgcgcccc agaccgacat cgacatgctg gtacctgccg tgctggaggg ggcgatcgcc tacgtcagtt ccggagcgac ggagttcctg gcggaagtca agacggtgat ggaacttggc gagcgttatc ccggcgacgc cggcgtgctg gcatcgttgt tgcttaaccg catcagcctc actcccggcg aggctatctt cttgccggcc ggcaacttgc atgcctattt gcgaggtgtt ggggtggagg tgatggctaa ctccgacaac gtgttgcgag gaggcctcac ccccaagcat gtcgatgtgc ctgagctgtt gcgggtgctg gatttcaacc caactcccga ggaggcgctg cgcccgccga cccgctgcga gggttttgcg gtggtttacg agaccccgac cgatgagttc gaggtagcgc tattggcgct cgacggcgat tatctgggtc acgaggtcga tgcgtcaccc cacgatgtgt cgcaaggccc acagatcttg ttgtgcaccg agggtgaaat tacggtgcac ggcaagttcc aggcactcac cgtagaacgc ggtatggctg tctggatggc ggccgacgac ggcccgattc ggctggtcgc gcagcaaccc gccaagctgt tcagggcaac cgtcggtttg tga

Details for MyleA.18263.a.A1.GE34175

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	46kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	81
PERCENT COVERAGE:	93

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVELLRGAL RTYAWGSRTA IAEFTGRPVP AAHPEAELWF GAHPGDSVWL QAANGETSLL EALVADPEGQ LGSVSRARFG DVLPFLVKVL AADEPLSLQA HPSTKQAVEG YLREERLGIP VSSPERNYRD TSHKPELLVA LQSFEALAGF RQSSRTAELL TALAVSDLDP FIHLLSDQSD ADGLRALFTT WITAPQTDID MLVPAVLEGA IAYVSSGATE FLAEVKTVME LGERYPGDAX VLASLLLNRI SLTPGEAIFL PAGNLHAYLX GVGVEVMANX DNVLRGGXTP XHVDVPXLLR VLXFNXTXEX XCAPXXRVCX XLXXPTMXXV XLXXXXXXXX GXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX

Validated NT Sequence

cnnnnnattt tntnnnnttt aagaaggaga tataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggtggaact gctacgcgga gcgttgcgga catatgcgtg gggatcgcgg accgctatcg ccgaattcac tggaagacct gtgccggctg ctcaccctga agctgagctc tggttcggtg cgcaccccgg tgactcggtt tggctacaag cagcgaacgg cgaaacttcg ctactggagg ctctggtcgc tgatccggaa gggcagcttg gctcagtgtc ccgcgctcga tttggtgacg tgctgccgtt cctggtcaag gtgctggcag ctgacgaacc gctgtcgctg caggcccatc cgagcaccaa gcaggcggtc gagggttacc tgcgcgaaga acgtctgggc attccggtgt cctcgccgga acgtaactac cgggatacca gtcacaagcc ggagttattg gtggcgctgc agtcattcga ggcgttggct ggatttcgtc agtcgtcacg cactgctgag ctactgacgg cgttagccgt ctccgacctc gaccctttca ttcatttgct gagcgaccag tccgacgccg acggtctgcg tgcgctgttc actacctgga taaccgcgcc ccagaccgac atcgacatgc tggtacctgc cgtgctggag ggggcgatcg cctacgtcag ttccggagcg acggagttcc tggcggaagt caagacggtg atggaacttg gcgagcgtta tcccggcgac gccngcgtgc tggcatcgtt gttgcttaac cgcatcagcc tcactcccgg cgaggctatc ttcttgccgg ccggcaactt gcatgcctat ttgcnaggtg ttggggtgga ggtgatggct aactncgaca acgtgttgcg aggaggcntn accccnnagc atgtcgatgt gcctganctg ttgcgggtgc tngntttcaa ccnnactncc gagnngnnct gcgcaccnnc tncnagggtt tgcnnnngnt tacnanaccc nacnatgnnt nnagtagnnc tantnnnnnn cgannnnnat natnnnggnn ncnnngncna tnnnnnnncn cnannnnnnn nnnnnnnnnn nnnatntnnn nnnnnnnncn gggnnnantn ngnnnnnnnn nnnnnnnntn nnnnnnnncn ncnnnnngnn nnggnnnnna nnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVELLRGAL RTYAWGSRTA IAEFTGRPVP AAHPEAELWF GAHPGDSVWL QAANGETSLL EALVADPEGQ LGSVSRARFG DVLPFLVKVL AADEPLSLQA HPSTKQAVEG YLREERLGIP VSSPERNYRD TSHKPELLVA LQSFEALAGF RQSSRTAELL TALAVSDLDP FIHLLSDQSD ADGLRALFTT WITAPQTDID MLVPAVLEGA IAYVSSGATE FLAEVKTVME LGERYPGDAG VLASLLLNRI SLTPGEAIFL PAGNLHAYLR GVGVEVMANS DNVLRGGLTP KHVDVPELLR VLDFNPTPEE ALRPPTRCEG FAVVYETPTD EFEVALLALD GDYLGHEVDA SPHDVSQGPQ ILLCTEGEIT VHGKFQALTV ERGMAVWMAA DDGPIRLVAQ QPAKLFRATV GL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggaactg ctacgcggag cgttgcggac atatgcgtgg ggatcgcgga ccgctatcgc cgaattcact ggaagacctg tgccggctgc tcaccctgaa gctgagctct ggttcggtgc gcaccccggt gactcggttt ggctacaagc agcgaacggc gaaacttcgc tactggaggc tctggtcgct gatccggaag ggcagcttgg ctcagtgtcc cgcgctcgat ttggtgacgt gctgccgttc ctggtcaagg tgctggcagc tgacgaaccg ctgtcgctgc aggcccatcc gagcaccaag caggcggtcg agggttacct gcgcgaagaa cgtctgggca ttccggtgtc ctcgccggaa cgtaactacc gggataccag tcacaagccg gagttattgg tggcgctgca gtcattcgag gcgttggctg gatttcgtca gtcgtcacgc actgctgagc tactgacggc gttagccgtc tccgacctcg accctttcat tcatttgctg agcgaccagt ccgacgccga cggtctgcgt gcgctgttca ctacctggat aaccgcgccc cagaccgaca tcgacatgct ggtacctgcc gtgctggagg gggcgatcgc ctacgtcagt tccggagcga cggagttcct ggcggaagtc aagacggtga tggaacttgg cgagcgttat cccggcgacg ccggcgtgct ggcatcgttg ttgcttaacc gcatcagcct cactcccggc gaggctatct tcttgccggc cggcaacttg catgcctatt tgcgaggtgt tggggtggag gtgatggcta actccgacaa cgtgttgcga ggaggcctca cccccaagca tgtcgatgtg cctgagctgt tgcgggtgct ggatttcaac ccaactcccg aggaggcgct gcgcccgccg acccgctgcg agggttttgc ggtggtttac gagaccccga ccgatgagtt cgaggtagcg ctattggcgc tcgacggcga ttatctgggt cacgaggtcg atgcgtcacc ccacgatgtg tcgcaaggcc cacagatctt gttgtgcacc gagggtgaaa ttacggtgca cggcaagttc caggcactca ccgtagaacg cggtatggct gtctggatgg cggccgacga cggcccgatt cggctggtcg cgcagcaacc cgccaagctg ttcagggcaa ccgtcggttt gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa