SSGCID: Target Page

MyleA.18253.a

PROBABLE TRANSCRIPTIONAL REGULATORY PROTEIN

CENTER ID:	MyleA.18253.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18253.a.A1.GE34371	full length		1	254

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1029
RefSeq:	YP_002503132.1
UniProt:	A0A0H3MUA4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLNVHRGYPS LDRSPELRHT GVVKIRSELD AATTVAVSDG HTRRAVVRLL LESGSITAGE ISDRLGLSAA GVRRHLDVLI EMGDAESATA APWQQVGRGR PAKHYRLTAV GRAKLGHAYD TLASAAMRQL REIGGEDAVR TFARQRIDAI LADVEPAGGG ADVEAAANRV ASALSDAGYV ATTARVGGPI HGVEICQHHC PVSHVAEEFP ELCETEQQAI AEVLGTHVQR LAMIVNGDCA CTTHVPLMSV PSKT

NT Sequence

gtgctcaacg ttcaccgagg gtacccttcc ctagaccgat cgccagaatt gcgtcacact ggtgttgtga aaatccggtc ggagctggat gctgctacga cagtcgcagt gtcggatggt cacactcgcc gcgcggtggt gcgcttgttg ctggagtccg ggtcgatcac cgccggtgaa atcagcgacc gtctgggtct gtcggccgca ggcgtgcgac gtcatctcga tgtgctcatc gagatgggcg acgctgagtc cgcgactgcg gcgccctggc agcaggttgg ccgcggccgc cccgccaagc actatcggct gaccgcggtg ggccgggcca agctcggtca tgcttacgac accttggcgt cggctgctat gcgacaactg cgggaaatcg gcggggagga cgctgttcgc acgttcgctc gacaacgtat cgacgcgatc ttggccgatg ttgagccagc cggtggcgga gccgacgttg aggcagccgc caaccgggtt gccagtgcgc tgagtgacgc cggttacgtc gcaaccaccg cgcgggtggg cggaccgatc cacggagtgg agatctgcca gcatcattgt ccagtgtccc atgtcgccga ggaattcccc gagttgtgtg aaaccgagca gcaggccatt gccgaggtgc tcggaaccca cgtgcagcgg ttggcgatga tcgtcaacgg agattgtgcc tgtactaccc acgtgccact catgtcggta ccaagcaaga cctag

Details for MyleA.18253.a.A1.GE34371

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/25/2012
EXPECTED MW:	29kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVLNVHRGY PSLDRSPELR HTGVVKIRSE LDAATTVAVS DGHTRRAVVR LLLESGSITA GEISDRLGLS AAGVRRHLDV LIEMGDAESA TAAPWQQVGR GRPAKHYRLT AVGRAKLGHA YDTLASAAMR QLREIGGEDA VRTFARQRID AILADVEPAG GGADVEAAAN RVASALSDAG YVATTARVGG PIHGVEICQH HCPVSHVAEE FPELCETEQQ AIAEVLGTHV QRLAMIVNGD CACTTHVPLM SVPS

Validated NT Sequence

tgcttggtac cgacatgagt ggcacgtggg tagtacaggc acaatctccg ttgacgatca tcgccaaccg ctgcacgtgg gttccgagca cctcggcaat ggcctgctgc tcggtttcac acaactcggg gaattcctcg gcgacatggg acactggaca atgatgctgg cagatctcca ctccgtggat cggtccgccc acccgcgcgg tggttgcgac gtaaccggcg tcactcagcg cactggcaac ccggttggcg gctgcctcaa cgtcggctcc gccaccggct ggctcaacat cggccaagat cgcgtcgata cgttgtcgag cgaacgtgcg aacagcgtcc tccccgccga tttcccgcag ttgtcgcata gcagccgacg ccaaggtgtc gtaagcatga ccgagcttgg cccggcccac cgcggtcagc cgatagtgct tggcggggcg gccgcggcca acctgctgcc agggcgccgc agtcgcggac tcagcgtcgc ccatctcgat gagcacatcg agatgacgtc gcacgcctgc ggccgacaga cccagacggt cgctgatttc accggcggtg atcgacccgg actccagcaa caagcgcacc accgcgcggc gagtgtgacc atccgacact gcgactgtcg tagcagcatc cagctccgac cggattttca caacaccagt gtgacgcaat tctggcgatc ggtctaggga agggtaccct cggtgaacgt tgagcaccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgctcaac gttcaccgag ggtacccttc cctagaccga tcgccagaat tgcgtcacac tggtgttgtg aaaatccggt cggagctgga tgctgctacg acagtcgcag tgtcggatgg tcacactcgc cgcgcggtgg tgcgcttgtt gctggagtcc gggtcgatca ccgccggtga aatcagcgac cgtctgggtc tgtcggccgc aggcgtgcga cgtcatctcg atgtgctcat cgagatgggc gacgctgagt ccgcgactgc ggcgccctgg cagcaggttg gccgcggccg ccccgccaag cactatcggc tgaccgcggt gggccgggcc aagctcggtc atgcttacga caccttggcg tcggctgcta tgcgacaact gcgggaaatc ggcggggagg acgctgttcg cacgttcgct cgacaacgta tcgacgcgat cttggccgat gttgagccag ccggtggcgg agccgacgtt gaggcagccg ccaaccgggt tgccagtgcg ctgagtgacg ccggttacgt cgcaaccacc gcgcgggtgg gcggaccgat ccacggagtg gagatctgcc agcatcattg tccagtgtcc catgtcgccg aggaattccc cgagttgtgt gaaaccgagc agcaggccat tgccgaggtg ctcggaaccc acgtgcagcg gttggcgatg atcgtcaacg gagattgtgc ctgtactacc cacgtgccac tcatgtcggt accaagcaag acctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa