SSGCID: Target Page

MyleA.18250.b

POSSIBLE ACYL-[ACYL-CARRIER PROTEIN] DESATURASE DESA2 (ACYL-[ACP] DESATURASE) (STEAROYL-ACP DESATURASE)

CENTER ID:	MyleA.18250.b
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18250.b.A1.GE34366	full length		1	338

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.4161
RefSeq:	YP_002504061.1
UniProt:	A0A0H3MRY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAELTDLQL LHELEPVVEK YMNRHERLHK NWNPHDYIPW SDGKNFYALG GQDWDPEQSQ LSDVAQVAMV QNLVTEDNLP SYHREIAMTM GMDGAWGQWV NRWTAEENRH GIALRDYLVV TRAVDPVELE KLRIEVVNRG FSPGQNHQGG LFADTLFDSI LYVTFQELAT RVSHRNTGKA CNETIADQLL AKISSDENLH MIFYRDVSEA GIELAPNLAI KALHKILHNF KMPGFLVPEF RRKAVIIAVG GVYDIRIHLD EVVMPVLKKW RILEREDFSG EGTRLRDEIG EHLTELEAAC DKFEVSKQRY IEREARRTEK ITARKVLSTE GTLRMSGR

NT Sequence

atgtcagccg agctgaccga cctacagctg ctgcacgagc tcgaaccggt cgtggagaag tacatgaacc gtcatgaacg cctacacaag aactggaatc cgcacgacta tatcccgtgg tcggatggca agaacttcta cgctctcggc ggtcaggatt gggaccccga gcagagccag ctctccgatg tcgcccaagt agcgatggta cagaacctgg tcaccgagga caacctgccg tcgtatcacc gagaaatcgc gatgaccatg ggcatggacg gtgcatgggg gcagtgggtc aaccgctgga cagccgagga aaaccggcac ggaatcgcgc tgcgcgacta tctggtggtg acccgcgcgg tcgatccggt ggagctagag aaactacgca tcgaggtagt caaccggggt ttcagcccgg gccagaatca ccaaggcgga ctcttcgccg acaccctgtt cgactcgatc ctctacgtca cattccagga gctggctacc cgcgtctcgc accgcaacac gggtaaggcc tgcaacgaga cgatcgctga ccagctgctc gctaaaatat cgagcgatga aaacctgcac atgatcttct accgggacgt cagcgaagct ggcatagagc tcgcacccaa ccttgcgatc aaggcactgc acaagatcct gcacaacttt aagatgccgg gattcctggt tcctgagttc cggcgtaagg ccgtgataat tgccgtcggt ggcgtctacg acattcggat ccaccttgac gaggtggtca tgccggtgct caaaaaatgg cggatcttgg agcgcgaaga cttcagcggc gagggtacac ggttgcgtga cgaaatcggc gagcatctta cagagcttga ggccgcgtgc gacaagttcg aagtctccaa gcagcgctac attgaacgcg aagcccgccg gactgagaaa atcaccgcac gcaaggtgct atctaccgaa ggcacgctga ggatgagtgg gcggtag

Details for MyleA.18250.b.A1.GE34366

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/13/2012
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	93
PERCENT COVERAGE:	72

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSAELTDLQ LLHELEPVVE KYMNRHERLH KNWNPHDYIP WSDGKNFYAL GGQDWDPEQS QLSDVAQVAM VQNLVTEDNL PSYHREIAMT MGMDGAWGQW VNRWTAEENR HGIALRDYLV VTRAVDPVEL EKLRIEVVNR GFSPGQNHQG GLFADTLFDS ILYVTFQELA TRVSHRNTGX XCNETIAXQL LAKISSDEXL HMIFYRDVSE XGIXLAPXLA IXXLHXILHN FXXAGXPGS

Validated NT Sequence

attttgnttn actttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgtca gccgagctga ccgacctaca gctgctgcac gagctcgaac cggtcgtgga gaagtacatg aaccgtcatg aacgcctaca caagaactgg aatccgcacg actatatccc gtggtcggat ggcaagaact tctacgctct cggcggtcag gattgggacc ccgagcagag ccagctctcc gatgtcgccc aagtagcgat ggtacagaac ctggtcaccg aggacaacct gccgtcgtat caccgagaaa tcgcgatgac catgggcatg gacggtgcat gggggcagtg ggtcaaccgc tggacagccg aggaaaaccg gcacggaatc gcgctgcgcg actatctggt ggtgacccgc gcggtcgatc cggtggagct agagaaacta cgcatcgagg tagtcaaccg gggtttcagc ccgggccaga atcaccaagg cggactcttc gccgacaccc tgttcgactc gatcctctac gtcacattcc aggagctggc tacccgcgtc tcgcaccgca acacgggtan ngnctgcaac gagacgatcg ctgancagct gctcgctaaa atatcgagcg atgaaannct gcacatgatc ttctaccggg acgtcagcga anctggcata nagctcgcac ccnaccttgc gatcnangna ctgcacnaga tcctgcacaa cttttnanan gccgggnttc ctggttcctg anttccggcg taaggccgtg ataattgccg tnnnnggcnn nnnacnacat tcnnancnnc nttgacnang nngtcatgcc nggngcncna aaaatgnnng nnntngganc gcnannnctt ncnncngnnn nggntacncn gnnnnnntga cnnanncngn nnnnnnatct tacanagctt gnnggcnnnn nnnnannann tnnnnaannc tnnnagcnnn nnnnnnnttn nannnnnnnn cnncnnnnnn gnnnanaann nnnncnnnan ggnncnnnnn nnncnaannn nnnnnnnnnn nnnnggncng annnnnnnnn nnannnnnnn nnnnnnnnnn nnnnngnnnn nnnnnnnncn nnnnnaannn annnnnantn ngnnnnnnnn nnnnnnnnnn c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSAELTDLQ LLHELEPVVE KYMNRHERLH KNWNPHDYIP WSDGKNFYAL GGQDWDPEQS QLSDVAQVAM VQNLVTEDNL PSYHREIAMT MGMDGAWGQW VNRWTAEENR HGIALRDYLV VTRAVDPVEL EKLRIEVVNR GFSPGQNHQG GLFADTLFDS ILYVTFQELA TRVSHRNTGK ACNETIADQL LAKISSDENL HMIFYRDVSE AGIELAPNLA IKALHKILHN FKMPGFLVPE FRRKAVIIAV GGVYDIRIHL DEVVMPVLKK WRILEREDFS GEGTRLRDEI GEHLTELEAA CDKFEVSKQR YIEREARRTE KITARKVLST EGTLRMSGR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcagccgag ctgaccgacc tacagctgct gcacgagctc gaaccggtcg tggagaagta catgaaccgt catgaacgcc tacacaagaa ctggaatccg cacgactata tcccgtggtc ggatggcaag aacttctacg ctctcggcgg tcaggattgg gaccccgagc agagccagct ctccgatgtc gcccaagtag cgatggtaca gaacctggtc accgaggaca acctgccgtc gtatcaccga gaaatcgcga tgaccatggg catggacggt gcatgggggc agtgggtcaa ccgctggaca gccgaggaaa accggcacgg aatcgcgctg cgcgactatc tggtggtgac ccgcgcggtc gatccggtgg agctagagaa actacgcatc gaggtagtca accggggttt cagcccgggc cagaatcacc aaggcggact cttcgccgac accctgttcg actcgatcct ctacgtcaca ttccaggagc tggctacccg cgtctcgcac cgcaacacgg gtaaggcctg caacgagacg atcgctgacc agctgctcgc taaaatatcg agcgatgaaa acctgcacat gatcttctac cgggacgtca gcgaagctgg catagagctc gcacccaacc ttgcgatcaa ggcactgcac aagatcctgc acaactttaa gatgccggga ttcctggttc ctgagttccg gcgtaaggcc gtgataattg ccgtcggtgg cgtctacgac attcggatcc accttgacga ggtggtcatg ccggtgctca aaaaatggcg gatcttggag cgcgaagact tcagcggcga gggtacacgg ttgcgtgacg aaatcggcga gcatcttaca gagcttgagg ccgcgtgcga caagttcgaa gtctccaagc agcgctacat tgaacgcgaa gcccgccgga ctgagaaaat caccgcacgc aaggtgctat ctaccgaagg cacgctgagg atgagtgggc ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa