SSGCID: Target Page

MyleA.18250.a

POSSIBLE ACYL-[ACYL-CARRIER PROTEIN] DESATURASE DESA2 (ACYL-[ACP] DESATURASE) (STEAROYL-ACP DESATURASE)

CENTER ID:	MyleA.18250.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.18250.a.A1.GE34367	full length		1	275

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3719
RefSeq:	YP_002503947.1
UniProt:	A0A0H3MVV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAQKPVPNAL ILQLEPVVKD NMARHFANEE LWFAHDYVPF DRGENFAFLG GRDWDPSQAT LAKAVTDACE ILLILKDNLA GYHRELVEHF ILEGWWGRWL GRWTAEEHLH AIALREYLVV TREVDPVANE QVRVEHVMKG YRVNSYTQIE TLVYMAFLER SYAFFCGSLA AQIKEPALFG LINQIVKDEV RHEEFFANLV AHCLECNRDE TVAAIAARAA GLDVLGADID AYHDKVENIS AAGIFGSVEL RQVISDRITA WGLINEPQLA QFVTS

NT Sequence

atggcacaga aacctgtacc taacgcattg atccttcagc ttgagcctgt ggtgaaagac aacatggctc gacacttcgc aaacgaggaa ctctggttcg cccacgacta cgtaccgttc gaccggggtg aaaactttgc cttccttggc ggccgcgatt gggatccgtc ccaggcgacg ttggccaagg ccgtcacgga cgcctgcgag atcctgctga ttcttaaaga caacctcgcc ggttatcacc gcgaactcgt cgagcatttc attctcgaag gttggtgggg tcgttggctc ggcaggtgga ccgcggagga gcatctgcat gccatcgcat tgcgcgaata cttggtagtg acccgagaag tcgatccggt agccaacgaa caggttcgag tcgagcacgt gatgaagggc taccgcgtca actcgtacac gcagatcgaa accttggtct acatggcgtt cctggagcgc agttacgcct ttttctgcgg cagcttggca gcgcagatta aggagcccgc tttgttcgga ctgatcaacc agatcgtcaa agacgaagta cgtcacgagg agttcttcgc caacctcgtc gcgcactgtc tggagtgcaa tcgcgacgag acggtcgccg cgattgccgc tcgtgcagcc ggtctagatg tcctcggtgc tgacattgac gcttaccacg acaaagtaga aaacatctcc gctgctggta ttttcggatc tgtcgaactg cggcaagtga tctccgaccg catcaccgca tggggtttga taaacgaacc gcaactggcg caattcgtca ccagctaa

Details for MyleA.18250.a.A1.GE34367

HARVESTED ON:	1/10/2012
SEQUENCED ON:	1/13/2012
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAQKPVPNA LILQLEPVVK DNMARHFANE ELWFAHDYVP FDRGENFAFL GGRDWDPSQA TLAKAVTDAC EILLILKDNL AGYHRELVEH FILEGWWGRW LGRWTAEEHL HAIALREYLV VTREVDPVAN EQVRVEHVMK GYRVNSYTQI ETLVYMAFLE RSYAFFCGSL AAQIKEPALF GLINQIVKDE VRHEEFFANL VAHCLECNRD ETVAAIAARA AGLDVLGADI DAYHDKVENI SAAGIFGSVE LRQVISDRIT AWGLINEPQL AQFVTS

Validated NT Sequence

attttgtttn actttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggca cagaaacctg tacctaacgc attgatcctt cagcttgagc ctgtggtgaa agacaacatg gctcgacact tcgcaaacga ggaactctgg ttcgcccacg actacgtacc gttcgaccgg ggtgaaaact ttgccttcct tggcggccgc gattgggatc cgtcccaggc gacgttggcc aaggccgtca cggacgcctg cgagatcctg ctgattctta aagacaacct cgccggttat caccgcgaac tcgtcgagca tttcattctc gaaggttggt ggggtcgttg gctcggcagg tggaccgcgg aggagcatct gcatgccatc gcattgcgcg aatacttggt agtgacccga gaagtcgatc cggtagccaa cgaacaggtt cgagtcgagc acgtgatgaa gggctaccgc gtcaactcgt acacgcagat cgaaaccttg gtctacatgg cgttcctgga gcgcagttac gcctttttct gcggcagctt ggcagcgcag attaaggagc ccgctttgtt cggactgatc aaccagatcg tcaaagacga agtacgtcac gaggagttct tcgccaacct cgtcgcgcac tgtctggagt gcaatcgcga cgagacggtc gccgcgattg ccgctcgtgc agccggtcta gatgtcctcg gtgctgacat tgacgcttac cacgacaaag tagaaaacat ctccgctgct ggtattttcg gatctgtcga actgcggcaa gtgatctccg accgcatcac cgcatggggt ttgataaacg aaccgcaact ggcgcaattc gtcaccagct aataaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgantng ctgctgc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacagaaa cctgtaccta acgcattgat ccttcagctt gagcctgtgg tgaaagacaa catggctcga cacttcgcaa acgaggaact ctggttcgcc cacgactacg taccgttcga ccggggtgaa aactttgcct tccttggcgg ccgcgattgg gatccgtccc aggcgacgtt ggccaaggcc gtcacggacg cctgcgagat cctgctgatt cttaaagaca acctcgccgg ttatcaccgc gaactcgtcg agcatttcat tctcgaaggt tggtggggtc gttggctcgg caggtggacc gcggaggagc atctgcatgc catcgcattg cgcgaatact tggtagtgac ccgagaagtc gatccggtag ccaacgaaca ggttcgagtc gagcacgtga tgaagggcta ccgcgtcaac tcgtacacgc agatcgaaac cttggtctac atggcgttcc tggagcgcag ttacgccttt ttctgcggca gcttggcagc gcagattaag gagcccgctt tgttcggact gatcaaccag atcgtcaaag acgaagtacg tcacgaggag ttcttcgcca acctcgtcgc gcactgtctg gagtgcaatc gcgacgagac ggtcgccgcg attgccgctc gtgcagccgg tctagatgtc ctcggtgctg acattgacgc ttaccacgac aaagtagaaa acatctccgc tgctggtatt ttcggatctg tcgaactgcg gcaagtgatc tccgaccgca tcaccgcatg gggtttgata aacgaaccgc aactggcgca attcgtcacc agctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa