MyleA.18233.a

NADPH:ADRENODOXIN OXIDOREDUCTASE FPRA (NADPH-FERREDOXIN REDUCTASE)

CENTER ID: MyleA.18233.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.18233.a.A1.GE34374 full length 1 456
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1188
RefSeq: YP_002503171.1
UniProt: A0A0H3MQ36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRPHHIHHIA IVGSGPSGFF AAASVLKAAD ASDEINVAVD MLEMLPTPWG LVRSGVAPDH PKIKSISKQF EKTAEDPRFR FFGNVIVGKH IEPAELAERY DAVIYAVGAQ SDRALNIPGE DLPGSIAAVD FVGWYNAHPN FHERSPDLSG SRAVVIGNGN VALDVTRILI TDPDVLAFTD IADHALESLR PRGIEEVVIV GRRGPLQTAF TTLELRELAD IEGVDVLVDP AQLEGISDEN AAAAGKTTRQ NIKVLRDYTV RTPKPGHRRI VFRFLTSPIE IKGKGKVERI VLGQNELVTD DNGRVAAKDT GVREELPAQL IVRSIGYRGV PTPGLPFDDS SVTIPNTNGR VNGSRNEYVV GWIKRGPTGV IGTNKKDSQD TVDTLMENLA GANDTEKFGA DHADRLAEWL AERQPKLVTS AHWQAIDRFE RAAGEPHGRP RVKLPNLAEL LRIGHG
NT Sequence
ttgcgtccac accacataca tcacatagcc atagttggct cggggccatc gggatttttc gccgcggcat ccgtgttgaa ggccgctgac gcctccgacg agatcaacgt tgccgtcgac atgctggaga tgctgcccac cccgtgggga ctggtccgct ctggtgtcgc acccgatcac cccaagatca aatcgatcag caagcagttc gagaagaccg ctgaggaccc gcggttccgc ttcttcggca atgtgattgt cggtaaacac atcgagcctg ccgaactcgc cgaacgctac gatgccgtga tatatgctgt cggggcgcag tccgaccgcg ctttgaacat ccccggtgaa gacctgccag gcagtatcgc cgctgtcgat ttcgtgggtt ggtataacgc acaccccaac ttccacgaga ggtcacccga cctttcgggc agtcgcgcag tggttattgg caacggcaac gtcgcactcg acgtcacacg catcctaatc accgatcccg acgtactggc attcaccgat atcgccgacc acgcactaga gtcactgcgc ccgcgcggga tcgaggaagt ggtgatcgtt ggccgccgcg ggccattaca gacagcgttc accacactgg agctgcgtga gctagcagac atcgaagggg tcgacgtgct tgtcgatcct gcccagctgg agggcatcag tgacgagaac gcggccgcgg cgggcaagac gaccaggcag aacatcaaag tgttacgcga ctacactgtg cgcacgccca aacccggcca tcgtcggatt gtgttccgat tcctgacttc cccaattgaa atcaaaggca aaggcaaggt ggagaggatc gttctcggtc aaaacgaact tgtcaccgac gacaacgggc gggtggcagc caaagacacc ggtgttcgcg aggagctgcc ggctcagttg attgtgcggt cgatcggtta tcgcggcgtc cctacgccgg ggttaccatt cgacgacagt agcgtaacca tccccaacac caacggccgg gtgaacggca gccgaaacga gtacgtcgtc gggtggatca aacgcgggcc gaccggggtg atcggcacca acaagaagga ctcccaagac actgtcgaca ccttgatgga gaatctggct ggcgccaatg ataccgaaaa gttcggcgca gaccacgccg accggctggc cgagtggctg gccgaacggc aaccgaagct ggtcacgtcg gcgcactggc aggccatcga ccgctttgag cgagcggccg gcgaaccgca cggccgcccc cgcgttaagc tgccgaacct cgccgagctg ctgcggatcg ggcacggctg a
Details for MyleA.18233.a.A1.GE34374
HARVESTED ON: 1/10/2012
SEQUENCED ON: 1/25/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 76
Validated AA Sequence
MLRPXHIXXX XXVGSGPXGX FAAASVLKAA DXSXEINVAV XMLEMLPTPX GLVRSGVAPD HPKIKXXXXX FEKTAEDPRF RFXXXVXVXX HIEPAELAER YDAVIYAVGA QSDRALNIPG EDLPGSIAAV DFVGXXNAHP NFHXRSPDLS GSRAVVIGNG XVALDVTRIX ITDPDVLAFT DIAXHALESL RPXGIEEVVI VGRRGPLQTA FTTLELRELA DIEGVDVLVD PAQLEGISDE NAAAAXKTTR QNIKVLRDYT VRTPKPGHRR IVFRFLTSPI EIKGKGKVER IVLGQNELVT DDNGRVAAKD TGVREELPAQ LIVRSIGYRG VPTPGLPFDD SSVTIPNTNG RVNGSRNEYV VGWIK
Validated NT Sequence
cgtttgatcc acccgacgac gtactcgttt cggctgccgt tcacccggcc gttggtgttg gggatggtta cgctactgtc gtcgaatggt aaccccggcg tagggacgcc gcgataaccg atcgaccgca caatcaactg agccggcagc tcctcgcgaa caccggtgtc tttggctgcc acccgcccgt tgtcgtcggt gacaagttcg ttttgaccga gaacgatcct ctccaccttg cctttgcctt tgatttcaat tggggaagtc aggaatcgga acacaatccg acgatggccg ggtttgggcg tgcgcacagt gtagtcgcgt aacactttga tgttctgcct ggtcgtcttg nccgccgcgg ccgcgttctc gtcactgatg ccctccagct gggcaggatc gacaagcacg tcgacccctt cgatgtctgc tagctcacgc agctccagtg tggtgaacgc tgtctgtaat ggcccgcggc ggccaacgat caccacttcc tcgatcccgn gngggcgcag tgactctagt gcgtggtngg cgatatcggt gaatgccagt acgtcgggat cggtgattng gatgcgtgtg acgtcgagtg cgacgtngcc gttgccaata accactgcgc gactgcccga aaggtcgggt gacctctngt ggaagttggg gtgtgcgtta nnnnnnccca cgaaatcgac agcggcgata ctgcctggca ggtcttcacc ggggatgttc aaagcgcggt cggactgcgc cccgacagca tatatcacgg catcgtagcg ttcggcgagt tcggcaggct cgatgtgnnn ncngacnnnc acatnnnnnn agaagcggaa ccgcgggtcc tcagcggtct tctcgaantg ctnnntnnnn nntttgatct tggggtgatc gggtgcgaca ccagagcgga ccagtccccn cggggtgggc agcatctcca gcatgtngac ggcaacgttg atctcgtngg aggngtcagc ggccttcaac acggatgccg cggcgaanaa ncccnanggc cccgagccaa ctanggntnn nnnangtatg tggngnggac gcaacatcga accaggaccc tgggtctgag cttccagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLRPHHIHH IAIVGSGPSG FFAAASVLKA ADASDEINVA VDMLEMLPTP WGLVRSGVAP DHPKIKSISK QFEKTAEDPR FRFFGNVIVG KHIEPAELAE RYDAVIYAVG AQSDRALNIP GEDLPGSIAA VDFVGWYNAH PNFHERSPDL SGSRAVVIGN GNVALDVTRI LITDPDVLAF TDIADHALES LRPRGIEEVV IVGRRGPLQT AFTTLELREL ADIEGVDVLV DPAQLEGISD ENAAAAGKTT RQNIKVLRDY TVRTPKPGHR RIVFRFLTSP IEIKGKGKVE RIVLGQNELV TDDNGRVAAK DTGVREELPA QLIVRSIGYR GVPTPGLPFD DSSVTIPNTN GRVNGSRNEY VVGWIKRGPT GVIGTNKKDS QDTVDTLMEN LAGANDTEKF GADHADRLAE WLAERQPKLV TSAHWQAIDR FERAAGEPHG RPRVKLPNLA ELLRIGHG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttgcgtcca caccacatac atcacatagc catagttggc tcggggccat cgggattttt cgccgcggca tccgtgttga aggccgctga cgcctccgac gagatcaacg ttgccgtcga catgctggag atgctgccca ccccgtgggg actggtccgc tctggtgtcg cacccgatca ccccaagatc aaatcgatca gcaagcagtt cgagaagacc gctgaggacc cgcggttccg cttcttcggc aatgtgattg tcggtaaaca catcgagcct gccgaactcg ccgaacgcta cgatgccgtg atatatgctg tcggggcgca gtccgaccgc gctttgaaca tccccggtga agacctgcca ggcagtatcg ccgctgtcga tttcgtgggt tggtataacg cacaccccaa cttccacgag aggtcacccg acctttcggg cagtcgcgca gtggttattg gcaacggcaa cgtcgcactc gacgtcacac gcatcctaat caccgatccc gacgtactgg cattcaccga tatcgccgac cacgcactag agtcactgcg cccgcgcggg atcgaggaag tggtgatcgt tggccgccgc gggccattac agacagcgtt caccacactg gagctgcgtg agctagcaga catcgaaggg gtcgacgtgc ttgtcgatcc tgcccagctg gagggcatca gtgacgagaa cgcggccgcg gcgggcaaga cgaccaggca gaacatcaaa gtgttacgcg actacactgt gcgcacgccc aaacccggcc atcgtcggat tgtgttccga ttcctgactt ccccaattga aatcaaaggc aaaggcaagg tggagaggat cgttctcggt caaaacgaac ttgtcaccga cgacaacggg cgggtggcag ccaaagacac cggtgttcgc gaggagctgc cggctcagtt gattgtgcgg tcgatcggtt atcgcggcgt ccctacgccg gggttaccat tcgacgacag tagcgtaacc atccccaaca ccaacggccg ggtgaacggc agccgaaacg agtacgtcgt cgggtggatc aaacgcgggc cgaccggggt gatcggcacc aacaagaagg actcccaaga cactgtcgac accttgatgg agaatctggc tggcgccaat gataccgaaa agttcggcgc agaccacgcc gaccggctgg ccgagtggct ggccgaacgg caaccgaagc tggtcacgtc ggcgcactgg caggccatcg accgctttga gcgagcggcc ggcgaaccgc acggccgccc ccgcgttaag ctgccgaacc tcgccgagct gctgcggatc gggcacggct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa