SSGCID: Target Page

MyleA.17987.a

DNA polymerase III subunit beta (EC 2.7.7.7)

CENTER ID:	MyleA.17987.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.17987.a.B1.GE41029	full length		1	399

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
UniProt:	A0A0H3MXP2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDLAKTNVGC SDLKFCLARE SFASAVSWVA KYLPTRPTVP VLSGVLLTGS DSGLTISGFD YEVSAEVQVA AEIASSGSVL VSGRLLSDIT RALPNKPVHF YVDGNRVALT CGSARFSLPT MAVEDYPTLP TLPDETGTLP SDVFAEAIGQ VAIAAGRDYT LPMLTGIRIE ISGDTVVLAA TDRFRLAVRE LKWSVLSSDF EASVLVPAKT LVEVAKAGTD GSGVCLSLGA GVGVGKDGLF GISGGGKRST TRLLDAEFPK FRQLLPAEHT AVATIDVAEL TEAIKLVALV ADRGAQVRME FGDGILRLSA GADDVGRAEE DLAVAFTGEP LTIAFNPNYL TDGLASVHSE RVSFGFTTPS KPALLRPTSN DDVHPTHDGP FPALPTDYVY LLMPVRLPG

NT Sequence

atggacctgg ccaaaaccaa tgttggttgc agcgatttaa aattttgttt agcacgtgag tcgttcgcca gcgcggtgtc atgggtagcg aagtatctcc ccactagacc aacggtaccg gtgctatccg gcgtgctgct gaccggttca gatagcggcc taacgatctc cggattcgat tacgaagttt ccgcagaggt gcaggttgct gccgagatag cttcttctgg aagcgttttg gtatctggga ggttgttgtc agatattact cgggcgcttc cgaacaaacc tgttcacttt tatgtggacg gtaatcgggt tgcattgact tgcggaagtg cgaggttctc gttgccgacg atggcggttg aagactaccc tacactgcct actttgccgg atgaaactgg cacgttgcca tcggatgtgt tcgctgaggc gataggtcag gttgctattg cggccggtcg tgactatacc ttgcccatgc ttaccgggat ccggatcgag atctcgggtg acacggtagt cttggccgcc accgataggt tccgtttggc ggtccgcgag ttaaaatggt cggtgttgtc atcggatttc gaggcatcgg tgctggtgcc ggccaaaact ttggtggaag ttgctaaagc cggtaccgac ggctctggtg tttgtctgtc attgggcgct ggagtcggcg ttggaaaaga tgggcttttc ggcattagcg gtggcgggaa gcgcagtact actcgacttc ttgacgctga gtttccgaaa ttcaggcagt tattgccggc tgagcacacc gcagtggcca ccatcgacgt ggctgagttg accgaggcga tcaaactggt ggcgttggta gctgaccgtg gcgcgcaagt gcgcatggaa ttcggtgacg gtatattacg gctttccgcc ggcgccgatg atgtgggccg agccgaagaa gatcttgctg ttgcttttac tggtgaaccg ttgactatag cgtttaaccc gaattatctg actgatggac ttgcatcggt gcattcagag cgggtgtcat ttggtttcac gacaccgagt aagccagcat tgctgcgtcc aacgtctaat gatgatgtcc atccgacgca tgatggcccc tttcccgcac tgccaactga ctatgtgtat ttactgatgc cagttcggtt gcctggataa

Details for MyleA.17987.a.B1.GE41029

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMD LAKTNVGCSD LKFCLARESF ASAVSWVAKY LPTRPTVPVL SGVLLTGSDS GLTISGFDYE VSAEVQVAAE IASSGSVLVS GRLLSDITRA LPNKPVHFYV DGNRVALTCG SARFSLPTMA VEDYPTLPTL PDETGTLPSD VFAEAIGQVA IAAGRDYTLP MLTGIRIEIS GDTVVLAATD RFRLAVRELK WSVLSSDFEA SVLVPAKTLV EVAKAGTDGS GVCLSLGAGV GVGKDGLFGI SGGGKRSTTR LLDAEFPKFR QLLPAEHTAV ATIDVAELTE AIKLVALVAD RGAQVRMEFG DGILRLSAGA DDVGRAEEDL AVAFTGEPLT IAFNPNYLTD GLASVHSERV SFGFTTPSKP ALLRPTSNDD VHPTHDGPFP ALPTDYVYL

Validated NT Sequence

gtaaatacac atagtcagtt ggcagtgcgg gaaaggggcc atcatgcgtc ggatggacat catcattaga cgttggacgc agcaatgctg gcttactcgg tgtcgtgaaa ccaaatgaca cccgctctga atgcaccgat gcaagtccat cagtcagata attcgggtta aacgctatag tcaacggttc accagtaaaa gcaacagcaa gatcttcttc ggctcggccc acatcatcgg cgccggcgga aagccgtaat ataccgtcac cgaattccat gcgcacttgc gcgccacggt cagctaccaa cgccaccagt ttgatcgcct cggtcaactc agccacgtcg atggtggcca ctgcggtgtg ctcagccggc aataactgcc tgaatttcgg aaactcagcg tcaagaagtc gagtagtact gcgcttcccg ccaccgctaa tgccgaaaag cccatctttt ccaacgccga ctccagcgcc caatgacaga caaacaccag agccgtcggt accggcttta gcaacttcca ccaaagtttt ggccggcacc agcaccgatg cctcgaaatc cgatgacaac accgaccatt ttaactcgcg gaccgccaaa cggaacctat cggtggcggc caagactacc gtgtcacccg agatctcgat ccggatcccg gtaagcatgg gcaaggtata gtcacgaccg gccgcaatag caacctgacc tatcgcctca gcgaacacat ccgatggcaa cgtgccagtt tcatccggca aagtaggcag tgtagggtag tcttcaaccg ccatcgtcgg caacgagaac ctcgcacttc cgcaagtcaa tgcaacccga ttaccgtcca cataaaagtg aacaggtttg ttcggaagcg cccgagtaat atctgacaac aacctcccag ataccaaaac gcttccagaa gaagctatct cggcagcaac ctgcacctct gcggaaactt cgtaatcgaa tccggagatc gttaggccgc tatctgaacc ggtcagcagc acgccggata gcaccggtac cgttggtcta gtggggagat acttcgctac ccatgacacc gcgctggcga acgactcacg tgctaaacaa aattttaaat cgctgcaacc aacattggtt ttggccaggt ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacc tggccaaaac caatgttggt tgcagcgatt taaaattttg tttagcacgt gagtcgttcg ccagcgcggt gtcatgggta gcgaagtatc tccccactag accaacggta ccggtgctat ccggcgtgct gctgaccggt tcagatagcg gcctaacgat ctccggattc gattacgaag tttccgcaga ggtgcaggtt gctgccgaga tagcttcttc tggaagcgtt ttggtatctg ggaggttgtt gtcagatatt actcgggcgc ttccgaacaa acctgttcac ttttatgtgg acggtaatcg ggttgcattg acttgcggaa gtgcgaggtt ctcgttgccg acgatggcgg ttgaagacta ccctacactg cctactttgc cggatgaaac tggcacgttg ccatcggatg tgttcgctga ggcgataggt caggttgcta ttgcggccgg tcgtgactat accttgccca tgcttaccgg gatccggatc gagatctcgg gtgacacggt agtcttggcc gccaccgata ggttccgttt ggcggtccgc gagttaaaat ggtcggtgtt gtcatcggat ttcgaggcat cggtgctggt gccggccaaa actttggtgg aagttgctaa agccggtacc gacggctctg gtgtttgtct gtcattgggc gctggagtcg gcgttggaaa agatgggctt ttcggcatta gcggtggcgg gaagcgcagt actactcgac ttcttgacgc tgagtttccg aaattcaggc agttattgcc ggctgagcac accgcagtgg ccaccatcga cgtggctgag ttgaccgagg cgatcaaact ggtggcgttg gtagctgacc gtggcgcgca agtgcgcatg gaattcggtg acggtatatt acggctttcc gccggcgccg atgatgtggg ccgagccgaa gaagatcttg ctgttgcttt tactggtgaa ccgttgacta tagcgtttaa cccgaattat ctgactgatg gacttgcatc ggtgcattca gagcgggtgt catttggttt cacgacaccg agtaagccag cattgctgcg tccaacgtct aatgatgatg tccatccgac gcatgatggc ccctttcccg cactgccaac tgactatgtg tatttactga tgccagttcg gttgcctgga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat