SSGCID: Target Page

MyleA.17943.a

Leucine--tRNA ligase (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS)

CENTER ID:	MyleA.17943.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.17943.a.B1.GE42455	full length		1	972

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
UniProt:	B8ZTR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTELPTTVPG YNSAVAQTDS DAMRYRYTAE LAGRIESTWQ DNWARLQTFN VPNPVGSLAP PDGSVVPADK LFVQDMFPYP SGDGLHVGHP LGYIATDVYA RYFRMTGHNV LHAMGFDAFG LPAEQYAMQT GTHPRILTEA NVVNFRHQLG RLGLGHDSRR TFSTTDVEFY KWTQWIFLQI YNAWFDVAAN KARPIAELIA EFDSGERRLV DGRDWATLSA GERADVIDNC RLVYRADSMV NWCPGLGTVL ANEEVTADGR SDRGNFPVFR KRLRQWMMRI TAYADRLLDD LDLLDWPEQV KTMQRNWIGR SSGATVLFSA ILSRSDAATT EVDVEVFTTR PDTMFGVTYL VLAPEHNLVD ELVATVWPDR TDPRWTYGAA TPGAAVAAYR RAIVAKSDLD RQESKEKTGV FLGRYATNPA TGKPVPIFVA DYVLVGYGTG AVMAVPGHDP RDWDFAHKFH LPIVEVIAGS DISEAAYVGD GVLVNSGYLD GMDVATAQEA ITARLESEGR GHARIEFKLR DWLFARQRYW GEPFPIIYDS DGRPHALDEA ALPVELPDVP YYSPVLFDPD DADSEPSPPL AKATEWVHVE LDLGDGLKPY SRDTNVMPQW AGSSWYELRY TDPHNSERLC AKENEAYWMG PRPTEHGIDD PGGVDLYVGG AEHAVLHLLY ARFWHKVLYD LGHVSSREPY RRLINQGYIQ AFAYTDAHGS YVPANQVFQR GDGFFCPGPD GEIEVFQEFG KIGKSLKNSV SPDEICDEYG ADTLRVYEMS MGPLEASRPW ATKDVVGAYR FLQRVWRLVV DERTGETRVV DTAGELDTYT LRTLHRTIAG VSQDYAALRN NTATAKLIEY TNHLTKEHRG SVPRVAVEPL VLMLAPLAPH LAEELWLRLG HTTSLANGPF PQADPAYLVD DTVEYPVQVN GKIRGRIVVA ADADYDTLKT VALADDKVQQ FLAGATPRKV IVVAGRLISL VI

NT Sequence

gtgactgaac tgccgaccac tgtccctggg tacaactcgg ctgttgccca gacggactcc gatgcgatgc gataccgcta taccgcggag ctagcaggcc gtatcgaaag cacctggcaa gacaactggg cgcggctgca gaccttcaac gtgcccaacc cggttgggtc gctagccccg ccggacggat cggtagttcc cgccgacaag ttgttcgtac aggacatgtt tccctatcca tcgggtgacg gattgcatgt tggacatcca ctaggttaca tcgccaccga cgtctacgct cgatacttcc gtatgaccgg tcataacgtg ctgcacgcga tggggttcga tgctttcggg ctgcccgccg aacagtacgc gatgcaaacc ggaacccacc cacgcattct aactgaggct aacgttgtca actttcggca ccagctgggc cggttgggtc ttggccacga cagccgacgc accttctcga ccaccgatgt cgagttctac aagtggaccc agtggatttt cctgcagatc tacaacgctt ggttcgacgt agcagctaac aaggcgcgcc cgatcgcaga attgattgcc gaattcgatt ccggtgagcg ccgccttgtt gacggccgtg actgggccac gttgtcggcc ggggagcggg ccgacgtgat cgacaactgt cgactggtct accgggcgga ctcgatggtc aactggtgtc cgggtctggg cacggtactg gccaacgaag aggtcaccgc cgacggtcgc agcgaccgcg gcaacttccc ggtattccgg aaacgtttga ggcaatggat gatgcggatc accgcctacg ctgaccggtt gctggacgac cttgacctgc tggactggcc ggagcaagtc aaaactatgc agcgcaactg gatcggccgt tccagcggag cgaccgtgct gttctcagcg atcctaagcc gcagcgatgc cgcgacaacc gaagtcgacg tcgaggtgtt caccacccgg ccggacacca tgtttggcgt cacctatctg gtgctggcac ccgagcacaa cctggtcgac gaactggttg ccaccgtctg gccggacagg acggaccccc ggtggacgta tggtgccgcc acgccaggtg cagctgtcgc agcctaccgc cgtgcaatcg ttgccaagtc ggacctcgat cgccaagaga gcaaggagaa gaccggcgtc ttcttgggta ggtacgccac caacccagcc accggaaaac cggtgccgat tttcgttgcc gactatgtac tggtcggcta tggtaccggt gcggtcatgg cggttcccgg ccatgacccg cgtgactggg acttcgccca taagttccat ttgccgatcg tcgaagttat tgctggaagc gatatttcag aggccgccta tgtgggcgat ggtgtgttgg tgaactccgg ttacctcgac ggtatggacg tggcaacagc ccaagaggcc attaccgccc gcttggagtc cgaaggtcga ggacatgctc gcatcgaatt caagctaaga gactggcttt tcgctcggca gcggtattgg ggtgagccgt tcccaataat ctatgatagt gacggacgac cgcatgcgct agacgaggcc gcactgccgg ttgagcttcc cgacgtaccg tactactctc cggtgttatt cgatcctgat gatgctgaca gcgagccatc acctccgctg gccaaggcga ccgagtgggt gcacgtagag ttggatctgg gcgacggttt gaagccttac agccgcgaca ccaatgtcat gccacagtgg gcgggcagct cttggtacga actgcgttac accgatccac acaactcaga gcggctctgt gccaaggaaa acgaggccta ttggatgggt ccacggccga ctgaacacgg catcgacgat cccggcggcg ttgatttgta cgtcggcgga gccgaacatg cggtgctaca tctgttgtat gcgaggtttt ggcacaaagt tctatacgat ctgggccacg tcagctctcg agaaccctac cgtaggctga tcaaccaggg ctacatccag gctttcgctt acactgatgc tcacggttca tatgtgccgg cgaaccaggt attccaacgc ggcgacgggt tcttctgtcc gggacccgac ggcgagatcg aagtcttcca agagtttggg aaaatcggta aaagcttgaa gaattcggta tcacctgacg aaatctgcga tgaatacggt gcagacacgt tgcgtgtgta tgaaatgtca atggggccgt tggaggcgtc ccgaccgtgg gccacgaagg acgtcgttgg cgcatatcgt ttccttcagc gggtgtggcg actggtggta gatgaacgca ctggtgaaac ccgggtggtg gacacagcag gggagttaga tacttacacg ttgcgaacat tgcaccgcac catcgcagga gtgtcccaag actatgctgc actgcgcaat aacaccgcaa cggcgaagct gatcgagtac accaaccatc tcaccaaaga acaccgtggc tcggtgcccc gggtcgctgt cgagcctctt gtgttgatgt tggcgccgct agccccacat ctggcagaag agctgtggct gcggctgggc cacaccacct ctttggcgaa cggccctttt ccccaggccg atcccgccta ccttgttgac gataccgttg aatacccggt gcaggtgaac ggcaagattc ggggtcggat agtggttgcc gccgacgcgg actacgacac cctgaaaact gttgcgttgg ctgacgataa agtacagcag tttttggccg gtgctacacc gcgcaaagtg attgtggtcg cgggccggct gatcagttta gtcatttaa

Details for MyleA.17943.a.B1.GE42455

HARVESTED ON:	5/25/2018
SEQUENCED ON:	5/31/2018
EXPECTED MW:	109kDa
OBSERVED MW:	109kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT ELPTTVPGYN SAVAQTDSDA MRYRYTAELA GRIESTWQDN WARLQTFNVP NPVGSLAPPD GSVVPADKLF VQDMFPYPSG DGLHVGHPLG YIATDVYARY FRMTGHNVLH AMGFDAFGLP AEQYAMQTGT HPRILTEANV VNFRHQLGRL GLGHDSRRTF STTDVEFYKW TQWIFLQIYN AWFDVAANKA RPIAELIAEF DSGERRLVDG RDWATLSAGE RADVIDNCRL VYRADSMVNW CPGLGTVLAN EEVTADGRSD RGNFPVFRKR LRQWMMRITA YADRLLDDLD LLDWPEQVKT MQRNWIGRSS GATVLFSAIL SRSDAATTEV DVEVFTTRPD TMFGVTYLVL APEHNLVDEL VATVWPDRTD PRWTYGAATP GAAVAAYRRA IVAKSDLDRQ ESKEKTGVFL GRYATNPATG KPVPIFVADY VLVGYGTGAV MAVPGHDPRD WDFAHKFHLP IVEVIAGSDI SEAAYVGDGV LVNSGYLDGM DVATAQEAIT ARLESEGRGH ARIEFKLRDW LFARQRYWGE PFPIIYDSDG RPHALDEAAL PVELPDVPYY SPVLFDPDDA DSEPSPPLAK ATEWVHVELD LGDGLKPYSR DTNVMPQWAG SSWYELRYTD PHNSERLCAK ENEAYWMGPR PTEHGIDDPG GVDLYVGGAE HAVLHLLYAR FWHKVLYDLG HVSSREPYRR LINQGYIQAF AYTDAHGSYV PANQVFQRGD GFFCPGPDGE IEVFQEFGKI GKSLKNSVSP DEICDEYGAD TLRVYEMSMG PLEASRPWAT KDVVGAYRFL QRVWRLVVDE RTGETRVVDT AGELDTYTLR TLHRTIAGVS QDYAALRNNT ATAKLIEYTN HLTKEHRGSV PRVAVEPLVL MLAPLAPHLA EELWLRLGHT TSLANGPFPQ ADPAYLVDDT VEYPVQVNGK IRGRIVVAAD ADYDTLKTVA LADDKVQQFL AGATPRKVIV VAGRLISLVI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgactg aactgccgac cactgtccct gggtacaact cggctgttgc ccagacggac tccgatgcga tgcgataccg ctataccgcg gagctagcag gccgtatcga aagcacctgg caagacaact gggcgcggct gcagaccttc aacgtgccca acccggttgg gtcgctagcc ccgccggacg gatcggtagt tcccgccgac aagttgttcg tacaggacat gtttccctat ccatcgggtg acggattgca tgttggacat ccactaggtt acatcgccac cgacgtctac gctcgatact tccgtatgac cggtcataac gtgctgcacg cgatggggtt cgatgctttc gggctgcccg ccgaacagta cgcgatgcaa accggaaccc acccacgcat tctaactgag gctaacgttg tcaactttcg gcaccagctg ggccggttgg gtcttggcca cgacagccga cgcaccttct cgaccaccga tgtcgagttc tacaagtgga cccagtggat tttcctgcag atctacaacg cttggttcga cgtagcagct aacaaggcgc gcccgatcgc agaattgatt gccgaattcg attccggtga gcgccgcctt gttgacggcc gtgactgggc cacgttgtcg gccggggagc gggccgacgt gatcgacaac tgtcgactgg tctaccgggc ggactcgatg gtcaactggt gtccgggtct gggcacggta ctggccaacg aagaggtcac cgccgacggt cgcagcgacc gcggcaactt cccggtattc cggaaacgtt tgaggcaatg gatgatgcgg atcaccgcct acgctgaccg gttgctggac gaccttgacc tgctggactg gccggagcaa gtcaaaacta tgcagcgcaa ctggatcggc cgttccagcg gagcgaccgt gctgttctca gcgatcctaa gccgcagcga tgccgcgaca accgaagtcg acgtcgaggt gttcaccacc cggccggaca ccatgtttgg cgtcacctat ctggtgctgg cacccgagca caacctggtc gacgaactgg ttgccaccgt ctggccggac aggacggacc cccggtggac gtatggtgcc gccacgccag gtgcagctgt cgcagcctac cgccgtgcaa tcgttgccaa gtcggacctc gatcgccaag agagcaagga gaagaccggc gtcttcttgg gtaggtacgc caccaaccca gccaccggaa aaccggtgcc gattttcgtt gccgactatg tactggtcgg ctatggtacc ggtgcggtca tggcggttcc cggccatgac ccgcgtgact gggacttcgc ccataagttc catttgccga tcgtcgaagt tattgctgga agcgatattt cagaggccgc ctatgtgggc gatggtgtgt tggtgaactc cggttacctc gacggtatgg acgtggcaac agcccaagag gccattaccg cccgcttgga gtccgaaggt cgaggacatg ctcgcatcga attcaagcta agagactggc ttttcgctcg gcagcggtat tggggtgagc cgttcccaat aatctatgat agtgacggac gaccgcatgc gctagacgag gccgcactgc cggttgagct tcccgacgta ccgtactact ctccggtgtt attcgatcct gatgatgctg acagcgagcc atcacctccg ctggccaagg cgaccgagtg ggtgcacgta gagttggatc tgggcgacgg tttgaagcct tacagccgcg acaccaatgt catgccacag tgggcgggca gctcttggta cgaactgcgt tacaccgatc cacacaactc agagcggctc tgtgccaagg aaaacgaggc ctattggatg ggtccacggc cgactgaaca cggcatcgac gatcccggcg gcgttgattt gtacgtcggc ggagccgaac atgcggtgct acatctgttg tatgcgaggt tttggcacaa agttctatac gatctgggcc acgtcagctc tcgagaaccc taccgtaggc tgatcaacca gggctacatc caggctttcg cttacactga tgctcacggt tcatatgtgc cggcgaacca ggtattccaa cgcggcgacg ggttcttctg tccgggaccc gacggcgaga tcgaagtctt ccaagagttt gggaaaatcg gtaaaagctt gaagaattcg gtatcacctg acgaaatctg cgatgaatac ggtgcagaca cgttgcgtgt gtatgaaatg tcaatggggc cgttggaggc gtcccgaccg tgggccacga aggacgtcgt tggcgcatat cgtttccttc agcgggtgtg gcgactggtg gtagatgaac gcactggtga aacccgggtg gtggacacag caggggagtt agatacttac acgttgcgaa cattgcaccg caccatcgca ggagtgtccc aagactatgc tgcactgcgc aataacaccg caacggcgaa gctgatcgag tacaccaacc atctcaccaa agaacaccgt ggctcggtgc cccgggtcgc tgtcgagcct cttgtgttga tgttggcgcc gctagcccca catctggcag aagagctgtg gctgcggctg ggccacacca cctctttggc gaacggccct tttccccagg ccgatcccgc ctaccttgtt gacgataccg ttgaataccc ggtgcaggtg aacggcaaga ttcggggtcg gatagtggtt gccgccgacg cggactacga caccctgaaa actgttgcgt tggctgacga taaagtacag cagtttttgg ccggtgctac accgcgcaaa gtgattgtgg tcgcgggccg gctgatcagt ttagtcattt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat