MyleA.17908.a

Demethylmenaquinone methyltransferase (EC 2.1.1.163)

CENTER ID: MyleA.17908.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.17908.a.B1.GE43626 full length 1 238
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
UniProt: A0A0H3MS48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPGSVDVVS RAALDKDPRD VVAMFDDVAH RYDLTNTVLS LGQDRYWRRA TRSALRIGPG QKVLDLAAGT AVSTAELSKS GAWCVAADFS VRMLATGGAR KVPKVAADAT QLPFSDGVFD AVTISFGLRN IADYQAALRE MARVTRPGGQ LVVCEFSTPT NALVANVYTE YLMRALPQVA RLVSSNPDAY IYLAESIRAW PDQVTLACQL SRTGWASPRW RNLTGGIVAL HAADKPVR
NT Sequence
ttgcctcccg gtagcgtcga cgtcgtgagt cgcgctgccc tggacaagga cccccgggat gtcgtagcga tgttcgacga cgtcgcccac cgatacgatc tgaccaacac tgtgctgtcg ctgggtcaag atcggtactg gcgacgagcc acgcggtcgg cgctgcggat tgggcccggg cagaaagtgc tggacctggc cgcaggcacg gcggtgtcca cagcggaact gagtaaatcc ggcgcctggt gtgtggctgc ggatttttcg gtcaggatgc tagcaaccgg cggtgcgcga aaggtaccta aggtggccgc agatgccacc caacttccct ttagcgatgg tgtattcgat gcagtcacta tcagtttcgg gttgcgcaat atcgccgact atcaggcggc gctgcgcgag atggcccgcg tcacccggcc cggcggtcaa ttagtagtgt gcgagttctc cacacccacc aatgcgctgg tcgccaatgt ctacacggaa tacctgatgc gggcactgcc gcaagtggcg cgactggtgt ctagtaatcc cgatgcctac atctacttgg cggagtccat cagagcctgg cccgatcagg ttacgctggc ctgccagctg tcgcggaccg gttgggcgtc gccgcgctgg cgtaacctca ccggcggaat tgtggctttg catgcagcag ataagccggt gcggtaa
Details for MyleA.17908.a.B1.GE43626
HARVESTED ON: 2/3/2020
SEQUENCED ON: 2/13/2020
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 94
PERCENT COVERAGE: 85
Validated AA Sequence
MAHHHHHHML PPGSVDVVSR AALDKDPRDV VAMFDDVAHR YDLTNTVLSL GQDRYWRRAT RSALRIGPGQ KVLDLAAGTA VSTAELSKSG AWCVAADFSV RMLATGGARK VPKVAADATQ LPFSDGVFDA VTISFGLRNI ADYQAALREM ARVTRPGGQL VVCEFSTPTN ALVANVYTEY LMRALPQVAR LVSSXSRCLH LLGGXHXXLA R
Validated NT Sequence
atggctcacc accaccacca ccatatgttg cctcccggta gcgtcgacgt cgtgagtcgc gctgccctgg acaaggaccc ccgggatgtc gtagcgatgt tcgacgacgt cgcccaccga tacgatctga ccaacactgt gctgtcgctg ggtcaagatc ggtactggcg acgagccacg cggtcggcgc tgcggattgg gcccgggcag aaagtgctgg acctggccgc aggcacggcg gtgtccacag cggaactgag taaatccggc gcctggtgtg tggctgcgga tttttcggtc aggatgctag caaccggcgg tgcgcgaaag gtacctaagg tggccgcaga tgccacccaa cttcccttta gcgatggtgt attcgatgca gtcactatca gtttcgggtt gcgcaatatc gccgactatc aggcggcgct gcgcgagatg gcccgcgtca cccggcccgg cggtcaatta gtagtgtgcg agttctccac acccaccaat gcgctggtcg ccaatgtcta cacggaatac ctgatgcggg cactgccgca agtggcgcga ctggtgtcta gtaantcccg atgcctacat ctacttggcg gantccatcn nancctggcc cgatc
Expected Protein Sequence
MAHHHHHHMP PGSVDVVSRA ALDKDPRDVV AMFDDVAHRY DLTNTVLSLG QDRYWRRATR SALRIGPGQK VLDLAAGTAV STAELSKSGA WCVAADFSVR MLATGGARKV PKVAADATQL PFSDGVFDAV TISFGLRNIA DYQAALREMA RVTRPGGQLV VCEFSTPTNA LVANVYTEYL MRALPQVARL VSSNPDAYIY LAESIRAWPD QVTLACQLSR TGWASPRWRN LTGGIVALHA ADKPVR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgcctc ccggtagcgt cgacgtcgtg agtcgcgctg ccctggacaa ggacccccgg gatgtcgtag cgatgttcga cgacgtcgcc caccgatacg atctgaccaa cactgtgctg tcgctgggtc aagatcggta ctggcgacga gccacgcggt cggcgctgcg gattgggccc gggcagaaag tgctggacct ggccgcaggc acggcggtgt ccacagcgga actgagtaaa tccggcgcct ggtgtgtggc tgcggatttt tcggtcagga tgctagcaac cggcggtgcg cgaaaggtac ctaaggtggc cgcagatgcc acccaacttc cctttagcga tggtgtattc gatgcagtca ctatcagttt cgggttgcgc aatatcgccg actatcaggc ggcgctgcgc gagatggccc gcgtcacccg gcccggcggt caattagtag tgtgcgagtt ctccacaccc accaatgcgc tggtcgccaa tgtctacacg gaatacctga tgcgggcact gccgcaagtg gcgcgactgg tgtctagtaa tcccgatgcc tacatctact tggcggagtc catcagagcc tggcccgatc aggttacgct ggcctgccag ctgtcgcgga ccggttgggc gtcgccgcgc tggcgtaacc tcaccggcgg aattgtggct ttgcatgcag cagataagcc ggtgcggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at