MyleA.17899.a

PROBABLE FOLYLPOLYGLUTAMATE SYNTHASE PROTEIN FOLC (FOLYLPOLY-GAMMA-GLUTAMATE SYNTHETASE) (FPGS)

CENTER ID: MyleA.17899.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.17899.a.A1.GE34208 full length 1 485
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2753
RefSeq: YP_002503666.1
UniProt: A0A0H3MVD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAELPDWGS GRLETNVAAL TPDKVASLLQ VEHLLDQRWP ETCIEPSLTR ISALMDVLGS PQLSYPSIHV AGTNGKTSVT RMVDALLTAL HQRTGRFTSP HLQSVVERIA IDSKPISPAQ YVATYREVEP FVQMIDAQSQ SSGGPALSKF EVVTAMAFAA FANAPVDIAV VEVGLGGRWD ATNVINASVA VITPVSIDHV EYLGNDVAAI AREKAGIITK APEGAPDTVV VIGRQVPAAM GVLLAQSAHA GARVVRQDSE FAVLDRQVAI GGQVLQLQGL GGVYNDIYLP LHGEHQAHNA VLVLAAVEAF YGAGVQRQLD VEAVRSGFAA VISPGRLERM RSAPTVFIDA AHNPAGANAL ARTLSDEFNF RTLVGVLSVL ANKDVDGILA ALELVLDYIV VTDNGSPRAL DVESLALVAR ERFGPDRVIS AANLRDAIDA ATALVDDAAA NEEIEGLGVS GRGIVITGSV ITAGIARTLF GRDPQ
NT Sequence
atgaccgcag agctgcctga ctggggatcc ggacgtcttg aaaccaatgt cgctgctctc acaccggaca aggtcgcctc cctgttgcag gttgaacacc tgctggacca gcgctggcca gagacctgta tcgaaccgag tttgactcgg atcagtgcgt taatggatgt tctcggctca ccgcaactca gttatccgtc gatacacgtc gctggcacca acgggaagac ctcggtgacg aggatggttg acgcgctgct aaccgcgctg caccagcgca ctggccgctt taccagccct cacctgcagt cggtagtcga acgcatcgcg attgactcaa aaccgatcag cccggcgcaa tatgtagcaa cttaccgtga ggttgaaccg tttgtgcaga tgatcgatgc ccagtcgcag tcctctgggg ggccggcgct gagcaagttc gaggtagtca ccgcgatggc gttcgccgcg ttcgcgaatg cgcccgtcga catcgcggtg gttgaggtgg gcctcggtgg gcgatgggat gcgaccaacg tgattaatgc atcggtcgcc gtgatcaccc cggtcagcat cgaccacgtc gagtatcttg gcaacgacgt tgcagcgatc gcccgcgaga aggcgggcat tatcacgaaa gctcccgaag gcgcgccaga caccgtcgtg gtaatcggtc gtcaggtacc tgcggcgatg ggggtgctgt tggctcaatc ggcacatgcc ggcgctcgag tcgtccgtca ggattcggag ttcgcggtct tggaccgtca ggtcgcaatc ggtggtcagg tgttgcagct gcaggggctt ggtggagttt ataatgacat ttacctgccg ttacatggcg aacaccaggc gcacaacgcg gtcttggtgc ttgctgcggt cgaggcgttt tatggtgccg gggtgcagcg ccagctcgat gtcgaggcgg tgcggtccgg tttcgccgct gtcatcagcc ccggcaggtt ggaacgcatg cgcagtgccc cgacggtgtt catcgacgcc gctcacaatc ccgccggagc gaatgcgttg gcgcgcacgc tgtctgacga atttaatttc cgaaccctgg tcggggtgct cagcgtgctg gcaaacaagg acgttgatgg catcctggcc gcactggagc tggtgctgga ttatatcgtt gtgactgaca acggctcgcc gcgggcgttg gatgttgagt cgctggcgtt ggtggcgcgg gagcggttcg gccccgaccg ggtaatcagc gcggcgaacc tgcgtgacgc tatcgacgca gcgactgcac tggtcgacga cgccgctgcc aatgaggaaa tcgaagggct cggagtctcc ggccgtggga tcgtcatcac cggctcggtg atcaccgccg gaatcgcacg gactttgttt ggccgtgacc cacaatga
Details for MyleA.17899.a.A1.GE34208
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/13/2011
EXPECTED MW: 53kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 74
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTAELPDWG SGRLETNVAA LTPDKVASLL QVEHLLDQRW PETCIEPSLT RISALMDVLG SPQLSYPSIH VAGTNGKTSV TRMVDALLTA LHQRTGRFTS PHLQSVVERI AIDSKPISPA QYVATYREVE PFVQMIDAQS QSSGGPALSK FEVVTAMAFA AFANAPVDIA VVEVGLGGRW DATNVINASV AVITPVSIDH VEYLGNDVAA IAREKAGIIT KAPEGAPDTV VVIGRQVPAA MGVLLAQSAH AGARVVRQDS EFAVLDRQVA IGGQVLQLQG LGGVYXDIYX XXXGEXXHXA XLXLAAVEXX MXXGXXXARX XXXXXXXXXV IXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
annnnatttt nnttancttt aagaaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgaccgcaga gctgcctgac tggggatccg gacgtcttga aaccaatgtc gctgctctca caccggacaa ggtcgcctcc ctgttgcagg ttgaacacct gctggaccag cgctggccag agacctgtat cgaaccgagt ttgactcgga tcagtgcgtt aatggatgtt ctcggctcac cgcaactcag ttatccgtcg atacacgtcg ctggcaccaa cgggaagacc tcggtgacga ggatggttga cgcgctgcta accgcgctgc accagcgcac tggccgcttt accagccctc acctgcagtc ggtagtcgaa cgcatcgcga ttgactcaaa accgatcagc ccggcgcaat atgtagcaac ttaccgtgag gttgaaccgt ttgtgcagat gatcgatgcc cagtcgcagt cctctggggg gccggcgctg agcaagttcg aggtagtcac cgcgatggcg ttcgccgcgt tcgcgaatgc gcccgtcgac atcgcggtgg ttgaggtggg cctcggtggg cgatgggatg cgaccaacgt gattaatgca tcggtcgccg tgatcacccc ggtcagcatc gaccacgtcg agtatcttgg caacgacgtt gcagcgatcg cccgcgagaa ggcgggcatt atcacgaaag ctcccgaagg cgcgccagac accgtcgtgg taatcggtcg tcaggtacct gcggcgatgg gggtgctgtt ggctcaatcg gcacatgccg gcgctcgagt cgtccgtcag gattcggagt tcgcggtctt ggaccgtcag gtcgcaatcg gtggtcaggt gttgcagctg caggggcttg gtggagttta tnatgacatt tacntgncgt nacntggnga anancnncac nacgcggnct tggngcttgc tgcngtcgag nntttnatgg ngncgggnnc ancnncngct cgatgnnnan nnntgcngnn cnnnnnnnnn nntgtcatca nccnnnnnnn nnnnnatgnn nnnnnncnnn gnnnnatnna nnnannncnn ncnnnnnnna tnnnnnnnnn nnnnncnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAELPDWG SGRLETNVAA LTPDKVASLL QVEHLLDQRW PETCIEPSLT RISALMDVLG SPQLSYPSIH VAGTNGKTSV TRMVDALLTA LHQRTGRFTS PHLQSVVERI AIDSKPISPA QYVATYREVE PFVQMIDAQS QSSGGPALSK FEVVTAMAFA AFANAPVDIA VVEVGLGGRW DATNVINASV AVITPVSIDH VEYLGNDVAA IAREKAGIIT KAPEGAPDTV VVIGRQVPAA MGVLLAQSAH AGARVVRQDS EFAVLDRQVA IGGQVLQLQG LGGVYNDIYL PLHGEHQAHN AVLVLAAVEA FYGAGVQRQL DVEAVRSGFA AVISPGRLER MRSAPTVFID AAHNPAGANA LARTLSDEFN FRTLVGVLSV LANKDVDGIL AALELVLDYI VVTDNGSPRA LDVESLALVA RERFGPDRVI SAANLRDAID AATALVDDAA ANEEIEGLGV SGRGIVITGS VITAGIARTL FGRDPQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcagag ctgcctgact ggggatccgg acgtcttgaa accaatgtcg ctgctctcac accggacaag gtcgcctccc tgttgcaggt tgaacacctg ctggaccagc gctggccaga gacctgtatc gaaccgagtt tgactcggat cagtgcgtta atggatgttc tcggctcacc gcaactcagt tatccgtcga tacacgtcgc tggcaccaac gggaagacct cggtgacgag gatggttgac gcgctgctaa ccgcgctgca ccagcgcact ggccgcttta ccagccctca cctgcagtcg gtagtcgaac gcatcgcgat tgactcaaaa ccgatcagcc cggcgcaata tgtagcaact taccgtgagg ttgaaccgtt tgtgcagatg atcgatgccc agtcgcagtc ctctgggggg ccggcgctga gcaagttcga ggtagtcacc gcgatggcgt tcgccgcgtt cgcgaatgcg cccgtcgaca tcgcggtggt tgaggtgggc ctcggtgggc gatgggatgc gaccaacgtg attaatgcat cggtcgccgt gatcaccccg gtcagcatcg accacgtcga gtatcttggc aacgacgttg cagcgatcgc ccgcgagaag gcgggcatta tcacgaaagc tcccgaaggc gcgccagaca ccgtcgtggt aatcggtcgt caggtacctg cggcgatggg ggtgctgttg gctcaatcgg cacatgccgg cgctcgagtc gtccgtcagg attcggagtt cgcggtcttg gaccgtcagg tcgcaatcgg tggtcaggtg ttgcagctgc aggggcttgg tggagtttat aatgacattt acctgccgtt acatggcgaa caccaggcgc acaacgcggt cttggtgctt gctgcggtcg aggcgtttta tggtgccggg gtgcagcgcc agctcgatgt cgaggcggtg cggtccggtt tcgccgctgt catcagcccc ggcaggttgg aacgcatgcg cagtgccccg acggtgttca tcgacgccgc tcacaatccc gccggagcga atgcgttggc gcgcacgctg tctgacgaat ttaatttccg aaccctggtc ggggtgctca gcgtgctggc aaacaaggac gttgatggca tcctggccgc actggagctg gtgctggatt atatcgttgt gactgacaac ggctcgccgc gggcgttgga tgttgagtcg ctggcgttgg tggcgcggga gcggttcggc cccgaccggg taatcagcgc ggcgaacctg cgtgacgcta tcgacgcagc gactgcactg gtcgacgacg ccgctgccaa tgaggaaatc gaagggctcg gagtctccgg ccgtgggatc gtcatcaccg gctcggtgat caccgccgga atcgcacgga ctttgtttgg ccgtgaccca caataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa