MyleA.17797.a

Probable exported protease

CENTER ID: MyleA.17797.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.17797.a.A2.GE34206 mature protein 31 511
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3086
RefSeq: YP_002503754.1
UniProt: A0A0H3MR29

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVAMPSLRSL SSALLSLGLL LQPAMTPPVV GASPEQTPSP VPEQNWGNCS VFLSDTSDIP SARCATVSVP VDYNNPDGVH AELAVIRAPA TGQRIGSLLF NPGGPGASAV DMVAEMIPGL QRTDIGRHFD LVGFDPRGVG HSTPALRCRT DVEFDAYRTE PMVDYSPAGV AHIEQVYKQL AQQCVARVGT AFLANVGTAS AARDMDIVRL ALGDEQINYL GYSYGTELGT AYLERFSDHV RAMVLDGAID PSVSSIQKDI QQMAGFQIAF TDYAADCARS ASCPLGTDPS QWVNRYHALI DPLVTKPGRT SDPRGLGYAD ATTGTINALY TPVHWKYLTS GLLGLQRGTD AGDLLLLADD YNGRDRNGHY TNDQDAFNAI RCVDAPVPTD SASWVSADQQ IRQAAPFLNY GQFTGNAPRD ICALWPVPAT SMPHPAPPVA PGKVVVVSTT HDPATPYQAG VDLARELSSP LITYDGTQHT AVFNGDQCVD SAVVHYFVDE TLLPASLQCQ P
NT Sequence
atggtagcca tgccgagcct gagatcgttg agctcggcgc tgctttcgtt agggctgttg ctgcaaccgg caatgacacc gccggtggtt ggtgccagcc cagagcagac tcctagcccg gtgccggagc agaactgggg taattgcagt gtgttcctgt ccgacactag cgacatcccc tcggcacggt gtgcaacggt gtcggtgccg gtcgactaca acaatcctga tggggtgcac gccgaattgg cggtcatccg cgctccagcg actggccagc ggatcggatc gctgctgttc aatccgggag gacccggggc gtccgcggtt gacatggtcg ccgaaatgat accagggcta caacgtaccg atattggccg tcacttcgac ctggtggggt tcgacccgcg aggagtcggc cactcaaccc cggcgcttcg gtgtcgcact gacgttgagt tcgacgcgta ccggaccgaa ccgatggttg actacagccc ggccggtgtg gcgcacatcg agcaagtcta caagcagctc gctcaacagt gtgttgcccg ggtcggcacc gcatttttgg cgaacgtggg aactgcctcg gccgcgcgcg acatggatat agttcgcctg gctctgggag acgagcagat caattacctc ggttatagct acggcaccga attgggtacc gcctatctcg agcggttcag cgaccatgtg cgggcgatgg tgcttgacgg cgctattgac ccgagtgtta gctcaatcca gaaagacatc cagcaaatgg ccggattcca gatcgcattt accgactatg ccgcggactg cgcccgctcg gcgtcctgtc cactgggcac tgaccccagc caatgggtca accgatacca tgcgctgatc gacccgttgg ttaccaagcc gggtcggacg tcggatccac gcggcctggg ctacgctgac gcgaccaccg gcaccattaa tgcgctctac accccggtgc actggaaata cctgaccagt ggcttgcttg ggctgcagcg tggcaccgac gccggtgacc tgctattgct cgccgacgac tacaacggcc gggaccgcaa tgggcactac accaacgacc aggacgcgtt taacgcgatc cgctgcgtcg acgcgccggt gccaaccgac tcggcgagct gggtatcggc cgatcagcag atacgccagg ccgctccgtt cctcaactac gggcaattca ccggcaacgc tccccgcgat atctgtgcac tgtggccggt gccggcgaca tcgatgccgc accccgcgcc gccggtcgcg ccgggcaagg ttgtcgtggt ctccacaacg cacgacccgg ccactccata tcaggccggg gtagacctgg ctcgcgagct gagcagcccg ctgatcacct acgacggaac ccagcacacc gcggtgttca acggcgacca gtgtgtggat agcgcggtgg tgcattattt cgtggacgag acgttgctac cggcgtcctt acaatgtcag ccctaa
Details for MyleA.17797.a.A2.GE34206
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 53kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMGASPEQTP SPVPEQNWGN CSVFLSDTSD IPSARCATVS VPVDYNNPDG VHAELAVIRA PATGQRIGSL LFNPGGPGAS AVDMVAEMIP GLQRTDIGRH FDLVGFDPRG VGHSTPALRC RTDVEFDAYR TEPMVDYSPA GVAHIEQVYK QLAQQCVARV GTAFLANVGT ASAARDMDIV RLALGDEQIN YLGYSYGTEL GTAYLERFSD HVRAMVLDGA IDPSVSSIQK DIQQMAGFQI AFTDYAADCA XSASCPLGTD PSQWXNRYHA LIDPXXQAGX XVXXTRLXTL XXXXAPXXXX TXRXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXGXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttttgtttac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgggtgc cagcccagag cagactccta gcccggtgcc ggagcagaac tggggtaatt gcagtgtgtt cctgtccgac actagcgaca tcccctcggc acggtgtgca acggtgtcgg tgccggtcga ctacaacaat cctgatgggg tgcacgccga attggcggtc atccgcgctc cagcgactgg ccagcggatc ggatcgctgc tgttcaatcc gggaggaccc ggggcgtccg cggttgacat ggtcgccgaa atgataccag ggctacaacg taccgatatt ggccgtcact tcgacctggt ggggttcgac ccgcgaggag tcggccactc aaccccggcg cttcggtgtc gcactgacgt tgagttcgac gcgtaccgga ccgaaccgat ggttgactac agcccggccg gtgtggcgca catcgagcaa gtctacaagc agctcgctca acagtgtgtt gcccgggtcg gcaccgcatt tttggcgaac gtgggaactg cctcggccgc gcgcgacatg gatatagttc gcctggctct gggagacgag cagatcaatt acctcggtta tagctacggc accgaattgg gtaccgccta tctcgagcgg ttcagcgacc atgtgcgggc gatggtgctt gacggcgcta ttgacccgag tgttagctca atccagaaag acatccagca aatggccgga ttccagatcg catttaccga ctatgccgcg gactgcgccc nctcggcgtc ctgtccactg ggcactgacc ccagccaatg gntcaaccga taccatgcgc tgatcgaccc nnnnnaccaa gccgggncgn angtcgnanc cacgcgnctg nntacnctga nnngacnnnc ngcaccattn nnnnnnnnta cancccgnnn nntgnaannn nctgannagn gntnnnngnn tgnnnnnnng nncnnannnn nnnacnnntn ttnnnnncna nnnnnnannn ncnggnnnnn cnatggnnnn nncnnnnnnn nnnnnnnnnn nnnnnntcnn nnnnnnnnnn nnnnnnnnna nngnnnnnnn nnnnnnnnnn nnnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGASPEQTP SPVPEQNWGN CSVFLSDTSD IPSARCATVS VPVDYNNPDG VHAELAVIRA PATGQRIGSL LFNPGGPGAS AVDMVAEMIP GLQRTDIGRH FDLVGFDPRG VGHSTPALRC RTDVEFDAYR TEPMVDYSPA GVAHIEQVYK QLAQQCVARV GTAFLANVGT ASAARDMDIV RLALGDEQIN YLGYSYGTEL GTAYLERFSD HVRAMVLDGA IDPSVSSIQK DIQQMAGFQI AFTDYAADCA RSASCPLGTD PSQWVNRYHA LIDPLVTKPG RTSDPRGLGY ADATTGTINA LYTPVHWKYL TSGLLGLQRG TDAGDLLLLA DDYNGRDRNG HYTNDQDAFN AIRCVDAPVP TDSASWVSAD QQIRQAAPFL NYGQFTGNAP RDICALWPVP ATSMPHPAPP VAPGKVVVVS TTHDPATPYQ AGVDLARELS SPLITYDGTQ HTAVFNGDQC VDSAVVHYFV DETLLPASLQ CQP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtgccagc ccagagcaga ctcctagccc ggtgccggag cagaactggg gtaattgcag tgtgttcctg tccgacacta gcgacatccc ctcggcacgg tgtgcaacgg tgtcggtgcc ggtcgactac aacaatcctg atggggtgca cgccgaattg gcggtcatcc gcgctccagc gactggccag cggatcggat cgctgctgtt caatccggga ggacccgggg cgtccgcggt tgacatggtc gccgaaatga taccagggct acaacgtacc gatattggcc gtcacttcga cctggtgggg ttcgacccgc gaggagtcgg ccactcaacc ccggcgcttc ggtgtcgcac tgacgttgag ttcgacgcgt accggaccga accgatggtt gactacagcc cggccggtgt ggcgcacatc gagcaagtct acaagcagct cgctcaacag tgtgttgccc gggtcggcac cgcatttttg gcgaacgtgg gaactgcctc ggccgcgcgc gacatggata tagttcgcct ggctctggga gacgagcaga tcaattacct cggttatagc tacggcaccg aattgggtac cgcctatctc gagcggttca gcgaccatgt gcgggcgatg gtgcttgacg gcgctattga cccgagtgtt agctcaatcc agaaagacat ccagcaaatg gccggattcc agatcgcatt taccgactat gccgcggact gcgcccgctc ggcgtcctgt ccactgggca ctgaccccag ccaatgggtc aaccgatacc atgcgctgat cgacccgttg gttaccaagc cgggtcggac gtcggatcca cgcggcctgg gctacgctga cgcgaccacc ggcaccatta atgcgctcta caccccggtg cactggaaat acctgaccag tggcttgctt gggctgcagc gtggcaccga cgccggtgac ctgctattgc tcgccgacga ctacaacggc cgggaccgca atgggcacta caccaacgac caggacgcgt ttaacgcgat ccgctgcgtc gacgcgccgg tgccaaccga ctcggcgagc tgggtatcgg ccgatcagca gatacgccag gccgctccgt tcctcaacta cgggcaattc accggcaacg ctccccgcga tatctgtgca ctgtggccgg tgccggcgac atcgatgccg caccccgcgc cgccggtcgc gccgggcaag gttgtcgtgg tctccacaac gcacgacccg gccactccat atcaggccgg ggtagacctg gctcgcgagc tgagcagccc gctgatcacc tacgacggaa cccagcacac cgcggtgttc aacggcgacc agtgtgtgga tagcgcggtg gtgcattatt tcgtggacga gacgttgcta ccggcgtcct tacaatgtca gccctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa