SSGCID: Target Page

MyleA.17674.a

CONSERVED HYPOTHETICAL PROTEIN. POSSIBLE TRIACYLGLYCEROL SYNTHASE (DIACYLGLYCEROL ACYLTRANSFERASE).

CENTER ID:	MyleA.17674.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.17674.a.A1.GE34205	full length		1	491

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2308
RefSeq:	YP_002503528.1
UniProt:	A0A0H3MV57

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADSVEGIGP FDELGALDYL LHRGEANPRT RAGIMAVELL DTTPDWNRFR SRIEDVSQRV LRLRQKVVVP TLPTAAPRWV VDPDFELDFH VRRVRVPDPG TLREVFDLAE VIQQSPMDVS RPLWTATLVE GLAAGRAAML LQISHAITDG VGSVEMFAEM YDLERDPPSR PRSPQPIPQD LSRNDLMLQG INHLPVALAG GVVGGLSGVA SVAGRAILRP ASTVSGVVGY VRSGIRVLSQ AAEPSPLLRQ RSLATRTEAI EIQLSDLHKA AKAGDGSIND AYLAGLVGAL RRYHEALGVS ISTLPMAVPV NVRTEADVVG SNRFVGVTLA APLGTNDPAA RMQKIRSQMT QRRDEPAMNI IGSLAPLMTV LPASVLDFIV DSVASSDVNA SNIPAYPGDT YFAGAKILRQ YGIGPRPGVA MMAVLMSRGG FCTVTVRYDR ASVKSEALFA RCLLEGFDEV LALAGDPTPH AVPASFAARS SGSPAGWLSS S

NT Sequence

atggctgact ccgtcgaggg cattggaccg ttcgatgaac tgggagcgct cgactatctg ctgcatcggg gtgaggcaaa tccacggacc cgcgccggga tcatggcggt ggaacttttg gataccacac cggactggaa tcggttccgg agccggatcg aggatgtctc ccaacgagtg ttgcggctgc ggcagaaggt tgtagtaccg acattgccta ccgccgcacc acgctgggtg gtggatcccg acttcgaact ggactttcat gtgcgtcggg tgcgcgtgcc tgatccgggt acgttgcgcg aggtattcga tcttgccgag gtgattcagc agtcaccgat ggacgtgtcg cggccactgt ggacggctac cttggtcgag ggcctggccg ctggcagggc tgcgatgcta ttgcagatta gccatgcgat caccgacggt gtcggcagtg tcgagatgtt cgccgaaatg tatgacttag aaagagatcc gccgtctaga cctcggtccc cgcaacctat tccgcaggat ctgtcgcgca atgacttaat gctacaaggc atcaaccact tgccggtcgc cctggcaggc ggtgtcgtgg gtgggctatc tggggtggcg tcggtggctg ggcgggcaat tctacgacct gcttcgactg tatcaggagt cgtcggttac gtccggtcgg ggatcagagt gttgagccag gctgccgaac cgtccccgtt gctgcgccag cgcagcctgg ctactcgcac cgaggcaata gagatccagc tctccgacct gcacaaggct gcgaaagcgg gcgatgggtc gattaatgac gcttatctgg ccggcttggt tggtgccttg cgccgctacc atgaggcact tggtgtgtcg atcagcacgc tgccgatggc ggtgccggta aacgtacgga ctgaagccga tgtggttgga agcaaccgat ttgttggcgt taccttggcg gcaccgctcg gtaccaatga tccggcagcc cggatgcaga agattcgttc gcagatgacg cagcggcgcg acgagcctgc aatgaacatc attggttccc tcgcaccgtt gatgacagta ctaccggcat cggtgctaga ttttatcgtt gactctgtgg ccagctccga tgtgaatgcc agtaatattc cggcctaccc cggggacacc tacttcgcgg gtgcaaaaat cttgcggcag tatggtatag gacctcgccc tggtgtagcg atgatggcgg tgctgatgtc tcgaggcggg ttctgcacgg tcactgtgcg atacgatcgg gcttcggtga aaagcgaggc attgtttgcc cggtgcctgc tggagggttt cgacgaggtc ctggcgttgg ccggtgaccc gacaccgcac gctgtgccgg catcgtttgc tgcgcggtcc tccggttcgc cggcgggatg gttgtcgagc tcatga

Details for MyleA.17674.a.A1.GE34205

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	61

Validated AA Sequence

Validated NT Sequence

attttgtnnn nnttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctg actccgtcga gggcattgga ccgttcgatg aactgggagc gctcgactat ctgctgcatc ggggtgaggc aaatccacgg acccgcgccg ggatcatggc ggtggaactt ttggatacca caccggactg gaatcggttc cggagccgga tcgaggatgt ctcccaacga gtgttgcggc tgcggcagaa ggttgtagta ccgacattgc ctaccgccgc accacgctgg gtggtggatc ccgacttcga actggacttt catgtgcgtc gggtgcgcgt gcctgatccg ggtacgttgc gcgaggtatt cgatcttgcc gaggtgattc agcagtcacc gatggacgtg tcgcggccac tgtggacggc taccttggtc gagggcctgg ccgctggcag ggctgcgatg ctattgcaga ttagccatgc gatcaccgac ggtgtcggca gtgtcgagat gttcgccgaa atgtatgact tagaaagaga tccgccgtct agacctcggt ccccgcaacc tattccgcag gatctgtcgc gcaatgactt aatgctacaa ggcatcaacc acttgccggt cgccctggca ggcggtgtcg tgggtgggct atctggggtg gcgtcggtgg ctgggcgggc aattctacga cctgcttcga ctgtatcnnn agtcgtcggt tacgtccggt cggggatcag agtgttgagc cnngctgccg aaccgtcccc gttgctgcgc cagcgcagcc tggctactcg caccgaggca atagagatcc agctctccga nctgcacaag gctgcnaaag cgggcgatgg gtcgattaat gacnntntct ggnngntnnn ngngccttgc gcnctnccat gaggnactgn gngtcnatca gnnngctncn atggcggngn cngnanngta cgnannnanc cnangnnnnn nnnnnnnttn ntnnnntnnn nnnnnncnnn tnngnncnnn annngnnanc ncnnnnnnan ntnnnnncnn nnnnnnnnnn ccannnnann ncnntngnnn nnnnnncnnt nnnnnnntnn cnntnnnnnn nnnnnnnnnn nnnnnnnnnn na

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMADSVEGIG PFDELGALDY LLHRGEANPR TRAGIMAVEL LDTTPDWNRF RSRIEDVSQR VLRLRQKVVV PTLPTAAPRW VVDPDFELDF HVRRVRVPDP GTLREVFDLA EVIQQSPMDV SRPLWTATLV EGLAAGRAAM LLQISHAITD GVGSVEMFAE MYDLERDPPS RPRSPQPIPQ DLSRNDLMLQ GINHLPVALA GGVVGGLSGV ASVAGRAILR PASTVSGVVG YVRSGIRVLS QAAEPSPLLR QRSLATRTEA IEIQLSDLHK AAKAGDGSIN DAYLAGLVGA LRRYHEALGV SISTLPMAVP VNVRTEADVV GSNRFVGVTL AAPLGTNDPA ARMQKIRSQM TQRRDEPAMN IIGSLAPLMT VLPASVLDFI VDSVASSDVN ASNIPAYPGD TYFAGAKILR QYGIGPRPGV AMMAVLMSRG GFCTVTVRYD RASVKSEALF ARCLLEGFDE VLALAGDPTP HAVPASFAAR SSGSPAGWLS SS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctgactcc gtcgagggca ttggaccgtt cgatgaactg ggagcgctcg actatctgct gcatcggggt gaggcaaatc cacggacccg cgccgggatc atggcggtgg aacttttgga taccacaccg gactggaatc ggttccggag ccggatcgag gatgtctccc aacgagtgtt gcggctgcgg cagaaggttg tagtaccgac attgcctacc gccgcaccac gctgggtggt ggatcccgac ttcgaactgg actttcatgt gcgtcgggtg cgcgtgcctg atccgggtac gttgcgcgag gtattcgatc ttgccgaggt gattcagcag tcaccgatgg acgtgtcgcg gccactgtgg acggctacct tggtcgaggg cctggccgct ggcagggctg cgatgctatt gcagattagc catgcgatca ccgacggtgt cggcagtgtc gagatgttcg ccgaaatgta tgacttagaa agagatccgc cgtctagacc tcggtccccg caacctattc cgcaggatct gtcgcgcaat gacttaatgc tacaaggcat caaccacttg ccggtcgccc tggcaggcgg tgtcgtgggt gggctatctg gggtggcgtc ggtggctggg cgggcaattc tacgacctgc ttcgactgta tcaggagtcg tcggttacgt ccggtcgggg atcagagtgt tgagccaggc tgccgaaccg tccccgttgc tgcgccagcg cagcctggct actcgcaccg aggcaataga gatccagctc tccgacctgc acaaggctgc gaaagcgggc gatgggtcga ttaatgacgc ttatctggcc ggcttggttg gtgccttgcg ccgctaccat gaggcacttg gtgtgtcgat cagcacgctg ccgatggcgg tgccggtaaa cgtacggact gaagccgatg tggttggaag caaccgattt gttggcgtta ccttggcggc accgctcggt accaatgatc cggcagcccg gatgcagaag attcgttcgc agatgacgca gcggcgcgac gagcctgcaa tgaacatcat tggttccctc gcaccgttga tgacagtact accggcatcg gtgctagatt ttatcgttga ctctgtggcc agctccgatg tgaatgccag taatattccg gcctaccccg gggacaccta cttcgcgggt gcaaaaatct tgcggcagta tggtatagga cctcgccctg gtgtagcgat gatggcggtg ctgatgtctc gaggcgggtt ctgcacggtc actgtgcgat acgatcgggc ttcggtgaaa agcgaggcat tgtttgcccg gtgcctgctg gagggtttcg acgaggtcct ggcgttggcc ggtgacccga caccgcacgc tgtgccggca tcgtttgctg cgcggtcctc cggttcgccg gcgggatggt tgtcgagctc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa