SSGCID: Target Page

MyleA.17369.a

PROBABLE ATP SYNTHASE BETA CHAIN ATPD

CENTER ID:	MyleA.17369.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.17369.a.A1.GE34204	full length		1	485

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2072
RefSeq:	YP_002503469.1
UniProt:	A0A0H3MQS4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSTTKTTKMT VKTGSKGTSG RVVRVTGPVV DVEFPHGFVP ELFNALNAKT TFSSLAKTLT LEVAQHLGDN LVRTISLQPT DGLVRGVEVT DTGNSISVPV GEGVKGHVFN ALGYCLDEPG YGDEFEHWSI HRKPPSFEEL EPRTEMLETG LKVVDLLTPY VRGGKIALFG GAGVGKTVLI QEMINRIARN FGGTSVFAGV GERTREGNDL WVELQEVNVL KDTALVFGQM DEPPGTRMRV ALSALTMAEW FRDEASQDVL LFIDNIFRFT QAGSEVSTLL GRMPSAVGYQ PTLADEMGEL QERITSTRGR SITSMQAVYV PADDYTDPAP ATTFAHLDAT TELSRSVFAK GIFPAVDPLA SSSTILDPGI VGEEHYRVAQ EVIRILQRYK DLQDIIAILG IDELSEEDKQ LVNRARRIER FLSQNMMAAE QFTGQPGSTV PVKETIDAFD RLCKGEFDHV PEQAFFLIGG LDDLTKKAES LGAKL

NT Sequence

atgtctacta ctaagacgac gaaaatgact gtaaagactg gaagcaaggg gaccagcggt cgcgtggtac gggttactgg tcctgtcgtc gacgtcgaat tcccgcacgg ttttgtcccg gagttgttca acgcgctaaa tgcgaagacc accttctcct cgcttgccaa gaccctcacg cttgaggttg cgcagcatct cggcgacaat ttagtgcgca ccatctcgtt gcagcctacc gacgggctgg tgcgcggagt tgaggtaacc gacactggta actcgatctc ggtgccggtt ggcgagggcg tcaagggtca tgtcttcaac gcgctggggt actgcctgga tgagcccggg tacggagacg agttcgagca ctggtcgatt caccgcaagc cgccttcctt cgaggagctt gagcctcgta ctgagatgct cgagaccggc ctgaaggtcg tggatttgct gactccgtat gtgcgtggcg gcaagatcgc cttgtttggc ggtgctggtg tgggcaagac agtgctaatc caggagatga tcaaccgcat cgcccgtaat ttcggtggta catcggtgtt cgccggagtt ggggagcgta ctcgcgaagg caatgacctg tgggtcgagc ttcaggaagt caacgtgctc aaggacaccg ccctggtgtt cggccagatg gatgagcctc ccggcacccg tatgcgggtg gcactgtccg cgctgacgat ggcggagtgg tttcgcgacg aggcgagcca ggatgttctg ctgttcatcg acaacatctt ccgattcacc caggctggat ccgaagtgtc gacgctgctc ggtcgtatgc cgtcggcggt agggtaccag cccacgctgg ctgacgagat gggtgagctg caggaacgca tcacctcgac acgaggccgg tcgatcacct cgatgcaggc cgtctatgtg cctgccgatg actacactga cccggcgccg gcaacgacgt tcgcgcacct ggacgccact actgagctgt cgcgttcggt gtttgccaag ggcatcttcc ctgcagtgga cccgctggcg tcgagctcta ccattctcga ccccggtatt gttggtgaag agcactaccg tgtggcccag gaagtcatcc ggattttgca gcgatacaaa gacctccagg atatcattgc gatccttggc atcgacgaac tttctgaaga ggacaagcaa ttggtcaacc gagctcggcg catcgagcgg tttctctctc agaacatgat ggcggctgaa caattcactg ggcagccggg ttcgacagtt ccggtgaagg agaccatcga cgcgttcgac cgcttgtgca agggagagtt cgaccatgtg cccgagcagg ccttcttctt gattggtggt ctcgacgact tgaccaagaa ggccgagagc ctcggcgcca agttgtga

Details for MyleA.17369.a.A1.GE34204

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	60

Validated AA Sequence

Validated NT Sequence

attttgntta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtcta ctactaagac gacgaaaatg actgtaaaga ctggaagcaa ggggaccagc ggtcgcgtgg tacgggttac tggtcctgtc gtcgacgtcg aattcccgca cggttttgtc ccggagttgt tcaacgcgct aaatgcgaag accaccttct cctcgcttgc caagaccctc acgcttgagg ttgcgcagca tctcggcgac aatttagtgc gcaccatctc gttgcagcct accgacgggc tggtgcgcgg agttgaggta accgacactg gtaactcgat ctcggtgccg gttggcgagg gcgtcaaggg tcatgtcttc aacgcgctgg ggtactgcct ggatgagccc gggtacggag acgagttcga gcactggtcg attcaccgca agccgccttc cttcgaggag cttgagcctc gtactgagat gctcgagacc ggcctgaagg tcgtggattt gctgactccg tatgtgcgtg gcggcaagat cgccttgttt ggcggtgctg gtgtgggcaa gacagtgcta atccaggaga tgatcaaccg catcgcccgt aatttcggtg gtacatcggt gttcgccgga gttggggagc gtactcgcga aggcaatgac ctgtgggtcg agcttcagga agtcaacgtg ctcaaggaca ccgccctggt gttcggccag atggatgagc ctcccggcac ccgtatgcgg gtggcactgt ccgcgctgac gatggcggan tggnttcgcg acgaggcgag ccaggatgtt ctgctgttca tcgacaacat cttccgattc acccaggctg gntccnaant gtcgacgctg ctcggtcgta tgccgtnnng gtagggtann agcccangct ggctgacgag atgggtnanc tgcnnnnnca tcncncgann cgannnnnga tcncnnnatg cnnnncnann tncnncnatn antnnnctgn nnnnngnnnn acnnnnnnnn nnnctnnnnn nnnnnnnnnn nnnnnnnnnn nnnntncnnn nnncnnnngg nnnnnnnnnn ncnnntnnnn nnnnnnanan nnnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSTTKTTKM TVKTGSKGTS GRVVRVTGPV VDVEFPHGFV PELFNALNAK TTFSSLAKTL TLEVAQHLGD NLVRTISLQP TDGLVRGVEV TDTGNSISVP VGEGVKGHVF NALGYCLDEP GYGDEFEHWS IHRKPPSFEE LEPRTEMLET GLKVVDLLTP YVRGGKIALF GGAGVGKTVL IQEMINRIAR NFGGTSVFAG VGERTREGND LWVELQEVNV LKDTALVFGQ MDEPPGTRMR VALSALTMAE WFRDEASQDV LLFIDNIFRF TQAGSEVSTL LGRMPSAVGY QPTLADEMGE LQERITSTRG RSITSMQAVY VPADDYTDPA PATTFAHLDA TTELSRSVFA KGIFPAVDPL ASSSTILDPG IVGEEHYRVA QEVIRILQRY KDLQDIIAIL GIDELSEEDK QLVNRARRIE RFLSQNMMAA EQFTGQPGST VPVKETIDAF DRLCKGEFDH VPEQAFFLIG GLDDLTKKAE SLGAKL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctactact aagacgacga aaatgactgt aaagactgga agcaagggga ccagcggtcg cgtggtacgg gttactggtc ctgtcgtcga cgtcgaattc ccgcacggtt ttgtcccgga gttgttcaac gcgctaaatg cgaagaccac cttctcctcg cttgccaaga ccctcacgct tgaggttgcg cagcatctcg gcgacaattt agtgcgcacc atctcgttgc agcctaccga cgggctggtg cgcggagttg aggtaaccga cactggtaac tcgatctcgg tgccggttgg cgagggcgtc aagggtcatg tcttcaacgc gctggggtac tgcctggatg agcccgggta cggagacgag ttcgagcact ggtcgattca ccgcaagccg ccttccttcg aggagcttga gcctcgtact gagatgctcg agaccggcct gaaggtcgtg gatttgctga ctccgtatgt gcgtggcggc aagatcgcct tgtttggcgg tgctggtgtg ggcaagacag tgctaatcca ggagatgatc aaccgcatcg cccgtaattt cggtggtaca tcggtgttcg ccggagttgg ggagcgtact cgcgaaggca atgacctgtg ggtcgagctt caggaagtca acgtgctcaa ggacaccgcc ctggtgttcg gccagatgga tgagcctccc ggcacccgta tgcgggtggc actgtccgcg ctgacgatgg cggagtggtt tcgcgacgag gcgagccagg atgttctgct gttcatcgac aacatcttcc gattcaccca ggctggatcc gaagtgtcga cgctgctcgg tcgtatgccg tcggcggtag ggtaccagcc cacgctggct gacgagatgg gtgagctgca ggaacgcatc acctcgacac gaggccggtc gatcacctcg atgcaggccg tctatgtgcc tgccgatgac tacactgacc cggcgccggc aacgacgttc gcgcacctgg acgccactac tgagctgtcg cgttcggtgt ttgccaaggg catcttccct gcagtggacc cgctggcgtc gagctctacc attctcgacc ccggtattgt tggtgaagag cactaccgtg tggcccagga agtcatccgg attttgcagc gatacaaaga cctccaggat atcattgcga tccttggcat cgacgaactt tctgaagagg acaagcaatt ggtcaaccga gctcggcgca tcgagcggtt tctctctcag aacatgatgg cggctgaaca attcactggg cagccgggtt cgacagttcc ggtgaaggag accatcgacg cgttcgaccg cttgtgcaag ggagagttcg accatgtgcc cgagcaggcc ttcttcttga ttggtggtct cgacgacttg accaagaagg ccgagagcct cggcgccaag ttgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa