MyleA.17295.a

ALPHA-MANNOSYLTRANSFERASE PIMA

CENTER ID: MyleA.17295.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.17295.a.A1.GE34202 full length 1 374
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.792
RefSeq: YP_002503037.1
UniProt: A0A0H3MPJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIGMICPYS FDVPGGVQSH VLQLAEVMRA RGQQVRVLAP ASPDVSLPEY VVSAGRAIPI PYNGSVARLQ FSPAVHSRVR RWLVDGDFDV LHLHEPNAPS LSMWALRVAE GPIVATFHTS TTKSLTLSVF QGVLRPWHEK IIGRIAVSDL ARRWQMEALG SDAVEIPNGV NVDSLSSAPQ LAGYPRLGKT VLFLGRYDEP RKGMSVLLDA LPGVMECFDD VQLLIVGRGD EEQLRSQAGG LVEHIRFLGQ VDDAGKAAAM RSADVYCAPN IGGESFGIVL VEAMAAGTPV VASDLDAFRR VLRDGEVGHL VPAGDSAALA DALVALLRND VLRERYVAAG AEAVRRYDWS VVASQIMRVY ETVATSGSKV QVAS
NT Sequence
atgcggatcg ggatgatctg tccatactcg ttcgatgtgc caggcggggt gcaatcgcac gtcttgcaac tggccgaggt gatgcgcgcg cggggacagc aggtccgtgt gctggcgccg gcttcgccgg atgtctcgct gcccgaatat gttgtctccg ccggcagggc gatcccgatt ccctacaacg gttcagtggc gcggctgcag ttcagtccgg cagtacacag cagagtcagg cgttggttag tggacggcga ttttgacgtg cttcacctgc acgaaccgaa cgcacccagc ctgtcgatgt gggcgctgcg ggtggccgag gggccaatcg tcgcgacatt tcatacctcc accaccaaat cactgacgct gtcggtattc caaggcgtgc tgcgaccatg gcacgagaag atcatcggcc ggatcgcagt atccgactta gcacggcgct ggcaaatgga ggctctgggg tccgatgcgg tggagatccc caacggggtg aacgttgatt cgttgtcctc ggcaccccag ctagccgggt acccgcggct cggcaagacg gtactgttcc tcggccgcta cgacgagcca cgcaaaggca tgtcggtcct gcttgatgcc ctaccgggag ttatggaatg cttcgacgac gtacagttac tgatcgtcgg ccgtggcgac gaagagcaat tgcggagcca ggcaggcgga ttggtggagc acatccgttt tcttggccag gtggatgacg ccggaaaagc cgcggcgatg cgcagcgccg acgtctattg cgcgcccaac atcggcggcg agagtttcgg cattgtcctg gtggaggcga tggccgccgg cactccggtg gtggcgagtg atctggacgc gtttcgccgc gtactgcgcg acggcgaagt cgggcattta gtgcctgcgg gcgatagcgc cgcgttggcc gatgcgcttg tcgcgttgct gcgcaacgac gtcctgcggg agcgttacgt ggccgccggc gccgaagcgg ttcgtcggta tgactggtcg gtggtggcca gccaaatcat gcgggtgtac gaaacggtcg ctacgtcagg ttccaaggtg caggtggcta gctga
Details for MyleA.17295.a.A1.GE34202
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRIGMICPY SFDVPGGVQS HVLQLAEVMR ARGQQVRVLA PASPDVSLPE YVVSAGRAIP IPYNGSVARL QFSPAVHSRV RRWLVDGDFD VLHLHEPNAP SLSMWALRVA EGPIVATFHT STTKSLTLSV FQGVLRPWHE KIIGRIAVSD LARRWQMEAL GSDAVEIPNG VNVDSLSSAP QLAGYPRLGK TVLFLGRYDE PRKGMSVLLD ALPGVMECFD DVQLLIVGRG DEEQLRSQAG GLVEHIRFLG QVDDAGKAAA MRSAXVYXXP NIGGXSFGIV LVEXMAXXTX XVASXLXVSX VLXXXXXXIX XXAXAXXXXX XXXCXXXXXX XXXXXXXXSX TXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttgttta ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgga tcgggatgat ctgtccatac tcgttcgatg tgccaggcgg ggtgcaatcg cacgtcttgc aactggccga ggtgatgcgc gcgcggggac agcaggtccg tgtgctggcg ccggcttcgc cggatgtctc gctgcccgaa tatgttgtct ccgccggcag ggcgatcccg attccctaca acggttcagt ggcgcggctg cagttcagtc cggcagtaca cagcagagtc aggcgttggt tagtggacgg cgattttgac gtgcttcacc tgcacgaacc gaacgcaccc agcctgtcga tgtgggcgct gcgggtggcc gaggggccaa tcgtcgcgac atttcatacc tccaccacca aatcactgac gctgtcggta ttccaaggcg tgctgcgacc atggcacgag aagatcatcg gccggatcgc agtatccgac ttagcacggc gctggcaaat ggaggctctg gggtccgatg cggtggagat ccccaacggg gtgaacgttg attcgttgtc ctcggcaccc cagctagccg ggtacccgcg gctcggcaag acggtactgt tcctcggccg ctacgacgag ccacgcaaag gcatgtcggt cctgcttgat gccctaccgg gagttatgga atgcttcgac gacgtacagt tactgatcgt cggccgtggc gacgaagagc aattgcggag ccaggcaggc ggattggtgg agcacatccg ttttcttggn caggtggatg acgccggaaa agccgcggcg atgcgcagcg ccnacgtcta ttgnncgccc aacatcggcg gcnagagttt cggcattgtc ctggtggagn nnatggccgn cnnnactnnn nnggtngcna gtganctgng cgtttcgcnc gtactgnncg angncnannn ngncatttnn ngcngngcna nngcncncnn ncnatgnnnt nnnnnntnnt gcnnnngncn ncnnnngnnn nntnnnnnnn nnnnnnnann nnnntcnnnn actgnnnngg ngcannatnn ngnnnnncna angnnnnncn ncnnnnnntt ngnnnnnnnn nnnnannnnn nnnnannaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIGMICPY SFDVPGGVQS HVLQLAEVMR ARGQQVRVLA PASPDVSLPE YVVSAGRAIP IPYNGSVARL QFSPAVHSRV RRWLVDGDFD VLHLHEPNAP SLSMWALRVA EGPIVATFHT STTKSLTLSV FQGVLRPWHE KIIGRIAVSD LARRWQMEAL GSDAVEIPNG VNVDSLSSAP QLAGYPRLGK TVLFLGRYDE PRKGMSVLLD ALPGVMECFD DVQLLIVGRG DEEQLRSQAG GLVEHIRFLG QVDDAGKAAA MRSADVYCAP NIGGESFGIV LVEAMAAGTP VVASDLDAFR RVLRDGEVGH LVPAGDSAAL ADALVALLRN DVLRERYVAA GAEAVRRYDW SVVASQIMRV YETVATSGSK VQVAS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggatcggg atgatctgtc catactcgtt cgatgtgcca ggcggggtgc aatcgcacgt cttgcaactg gccgaggtga tgcgcgcgcg gggacagcag gtccgtgtgc tggcgccggc ttcgccggat gtctcgctgc ccgaatatgt tgtctccgcc ggcagggcga tcccgattcc ctacaacggt tcagtggcgc ggctgcagtt cagtccggca gtacacagca gagtcaggcg ttggttagtg gacggcgatt ttgacgtgct tcacctgcac gaaccgaacg cacccagcct gtcgatgtgg gcgctgcggg tggccgaggg gccaatcgtc gcgacatttc atacctccac caccaaatca ctgacgctgt cggtattcca aggcgtgctg cgaccatggc acgagaagat catcggccgg atcgcagtat ccgacttagc acggcgctgg caaatggagg ctctggggtc cgatgcggtg gagatcccca acggggtgaa cgttgattcg ttgtcctcgg caccccagct agccgggtac ccgcggctcg gcaagacggt actgttcctc ggccgctacg acgagccacg caaaggcatg tcggtcctgc ttgatgccct accgggagtt atggaatgct tcgacgacgt acagttactg atcgtcggcc gtggcgacga agagcaattg cggagccagg caggcggatt ggtggagcac atccgttttc ttggccaggt ggatgacgcc ggaaaagccg cggcgatgcg cagcgccgac gtctattgcg cgcccaacat cggcggcgag agtttcggca ttgtcctggt ggaggcgatg gccgccggca ctccggtggt ggcgagtgat ctggacgcgt ttcgccgcgt actgcgcgac ggcgaagtcg ggcatttagt gcctgcgggc gatagcgccg cgttggccga tgcgcttgtc gcgttgctgc gcaacgacgt cctgcgggag cgttacgtgg ccgccggcgc cgaagcggtt cgtcggtatg actggtcggt ggtggccagc caaatcatgc gggtgtacga aacggtcgct acgtcaggtt ccaaggtgca ggtggctagc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa