MyleA.17268.a

PE FAMILY PROTEIN. POSSIBLE LIPASE.

CENTER ID: MyleA.17268.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.17268.a.A2.GE34195 mature protein 27 358
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2275
RefSeq: YP_002503523.1
UniProt: A0A0H3MV53

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRLLATTLA ALTVGTVSGF GSTVASSEPG EPWLPPSPVP VRENSPAKIV YALGGARPPT FDWDYYTIRA GDEFFPDVNR KLIDYPARAP FRYVPTFLVP GPRDEVTIGE AIAVATKNLN QAIHRGTEPA AVVGLSQGSL ALDTEQEQLA TDPTAPPPDQ LTFNTFGDPS GYHGFGKSVL ASIFRPGDYI PLIDYTMPQR MDSQYDSNRV VAAYDGLSEF PDRADNLLAQ LNCFAGGAIS HTPSGFFNPE DVPPQNIRTT VNSRGAKTTT YLIPVNHLPL TLPLRYLGWS DALVDQIDAV LQPKIDAAYA YNDNPLNKPI SVDPVNGMDP IAGIDAELRD SILNVFAQLR SILPPPPG
NT Sequence
atgaaacgac tactggctac gaccctcgcc gccttgaccg tgggtacggt tagtggattt ggatccaccg tcgcatcaag cgaacccggg gagccatggc tgccacccag cccggttccg gtgcgtgaga actcacccgc caaaatcgtc tatgcgctag gcggcgcgag accaccgacc tttgactggg attactacac catccgggcc ggcgacgagt ttttcccgga cgtcaaccgc aaactcatcg actaccccgc cagagcccct tttcgctatg tgccaacatt tctggtaccg gggcctcgag acgaagtgac tatcggcgaa gcgattgccg tagcaaccaa aaacctaaac caagccattc accgggggac cgagccggcc gctgtcgtcg gtttgtccca gggtagcctt gccctcgaca cagaacagga acagctcgcc actgacccga cggcaccacc accagaccag ttaacgttca acaccttcgg tgacccctca ggataccacg gattcggcaa gagtgtgctt gccagcatct tccggcccgg ggactatatc ccgctgatcg actacacgat gccccagcgt atggacagcc agtacgacag caacagagtt gtcgccgcat acgatgggct gtccgaattt cctgaccggg cagacaacct gttagcccag ttgaattgct tcgctggggg agcaatctcg cacactccat ccggattctt caatcctgaa gatgtgcccc cgcagaacat caggaccact gttaactcgc ggggcgccaa aacaacgacg tatttaattc cggtgaatca tcttccgttg acgttgccac tgcgctacct cggctggtcc gacgctttgg tggatcaaat cgatgcggtg ctacagccga aaatcgatgc cgcgtatgcg tacaacgata atcccttaaa caagccgatt tccgtagatc ctgtgaacgg catggacccg atcgccggca tcgacgcgga gctccgcgac agcatcctga atgttttcgc ccagctccgc tcgatccttc caccgccccc gggttga
Details for MyleA.17268.a.A2.GE34195
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 38kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 90
PERCENT COVERAGE: 103
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSEPGEPWL PPSPVPVREN SPAKIVYALG GARPPTFDWD YYTIRAGDEF FPDVNRKLID YPARAPFRYV PTFLVPGPRD EVTIGEAIAV ATKNLNQAIH RGTEPAAVVG LSQGSLALDT EQEQLATDPT APPPDQLTFN TFGDPSGYHG FGKSVLASIF RPGDYIPLID YTMPQRMDSQ YDSNRVVAAY DGLSEFPDRA DNLLAQLNCF AGGAISHTPS GFFNPEDVPP QNIRTTVNSR GAKTTTYLIP VNHLPLTLPL RYLGWSDALV DQIDAXLQPK IDAAYAYXDX PLNKPISVXP XTXWTRSXAS XXXXTAXXXF AXXXXXXXXP XXXXXXXXAX XXXXXXXX
Validated NT Sequence
tttgnttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgagcga acccggggag ccatggctgc cacccagccc ggttccggtg cgtgagaact cacccgccaa aatcgtctat gcgctaggcg gcgcgagacc accgaccttt gactgggatt actacaccat ccgggccggc gacgagtttt tcccggacgt caaccgcaaa ctcatcgact accccgccag agcccctttt cgctatgtgc caacatttct ggtaccgggg cctcgagacg aagtgactat cggcgaagcg attgccgtag caaccaaaaa cctaaaccaa gccattcacc gggggaccga gccggccgct gtcgtcggtt tgtcccaggg tagccttgcc ctcgacacag aacaggaaca gctcgccact gacccgacgg caccaccacc agaccagtta acgttcaaca ccttcggtga cccctcagga taccacggat tcggcaagag tgtgcttgcc agcatcttcc ggcccgggga ctatatcccg ctgatcgact acacgatgcc ccagcgtatg gacagccagt acgacagcaa cagagttgtc gccgcatacg atgggctgtc cgaatttcct gaccgggcag acaacctgtt agcccagttg aattgcttcg ctgggggagc aatctcgcac actccatccg gattcttcaa tcctgaagat gtgcccccgc agaacatcag gaccactgtt aactcgcggg gcgccaaaac aacgacgtat ttaattccgg tgaatcatct tccgttgacg ttgccactgc gctacctcgg ctggtccgac gctttggtgg atcaaatcga tgcggngcta cagccgaaaa tcgatgccgc gtatgcntac ancgatantc ccttaaacaa gccgatttcc gtanatcctg nnacngnatg gacccgatcg ncngcntcga nnnnnnntnc gacngcancn natgntttcg ccagnnncnc nngancnnnn ncnnncccgn nnntaannng nnnnannnan tntgcannnn ntnncnnnnn nnnnngctan nanc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSEPGEPWL PPSPVPVREN SPAKIVYALG GARPPTFDWD YYTIRAGDEF FPDVNRKLID YPARAPFRYV PTFLVPGPRD EVTIGEAIAV ATKNLNQAIH RGTEPAAVVG LSQGSLALDT EQEQLATDPT APPPDQLTFN TFGDPSGYHG FGKSVLASIF RPGDYIPLID YTMPQRMDSQ YDSNRVVAAY DGLSEFPDRA DNLLAQLNCF AGGAISHTPS GFFNPEDVPP QNIRTTVNSR GAKTTTYLIP VNHLPLTLPL RYLGWSDALV DQIDAVLQPK IDAAYAYNDN PLNKPISVDP VNGMDPIAGI DAELRDSILN VFAQLRSILP PPPG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgaaccc ggggagccat ggctgccacc cagcccggtt ccggtgcgtg agaactcacc cgccaaaatc gtctatgcgc taggcggcgc gagaccaccg acctttgact gggattacta caccatccgg gccggcgacg agtttttccc ggacgtcaac cgcaaactca tcgactaccc cgccagagcc ccttttcgct atgtgccaac atttctggta ccggggcctc gagacgaagt gactatcggc gaagcgattg ccgtagcaac caaaaaccta aaccaagcca ttcaccgggg gaccgagccg gccgctgtcg tcggtttgtc ccagggtagc cttgccctcg acacagaaca ggaacagctc gccactgacc cgacggcacc accaccagac cagttaacgt tcaacacctt cggtgacccc tcaggatacc acggattcgg caagagtgtg cttgccagca tcttccggcc cggggactat atcccgctga tcgactacac gatgccccag cgtatggaca gccagtacga cagcaacaga gttgtcgccg catacgatgg gctgtccgaa tttcctgacc gggcagacaa cctgttagcc cagttgaatt gcttcgctgg gggagcaatc tcgcacactc catccggatt cttcaatcct gaagatgtgc ccccgcagaa catcaggacc actgttaact cgcggggcgc caaaacaacg acgtatttaa ttccggtgaa tcatcttccg ttgacgttgc cactgcgcta cctcggctgg tccgacgctt tggtggatca aatcgatgcg gtgctacagc cgaaaatcga tgccgcgtat gcgtacaacg ataatccctt aaacaagccg atttccgtag atcctgtgaa cggcatggac ccgatcgccg gcatcgacgc ggagctccgc gacagcatcc tgaatgtttt cgcccagctc cgctcgatcc ttccaccgcc cccgggttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa