MyleA.01563.a

PROBABLE DIHYDRODIPICOLINATE SYNTHASE DAPA (DHDPS) (DIHYDRODIPICOLINATE SYNTHETASE)

CENTER ID: MyleA.01563.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01563.a.A1.GE34136 full length 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2848
RefSeq: YP_002503690.1
UniProt: B8ZRR3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTVGFDVPA RLGTLLTAMV TPFDADGSVD TAAATRLANR LVDAGCDGLV LSGTTGESPT TTDDEKLQLL RVVLEAVGDR ARVIAGAGSY DTAHSVRLVK ACAGEGAHGL LVVTPYYSKP PQTGLFAHFT AVADATELPV LLYDIPGRSV VPIEPDTIRA LASHPNIVGV KEAKADLYSG ARIMADTGLA YYSGDDALNL PWLAVGAIGF ISVISHLAAG QLRELLSAFG SGDITTARKI NVAIGPLCSA MDRLGGVTMS KAGLRLQGID VGDPRLPQMP ATAEQIDELA VDMRAASVLR
NT Sequence
gtgaccactg tcggattcga cgtccccgca cgtttgggga ccctgcttac tgcgatggtg acaccgtttg acgctgatgg ttctgttgac actgcggctg cgacgcggct ggcgaaccgc ctggtcgacg cgggttgtga tggtctggtg ctctcgggca ccaccggcga gtcgccgacc actactgacg acgagaaact ccaactgttg cgtgtcgtac ttgaggcggt aggtgaccga gctagagtca tcgccggcgc aggtagttat gacacagctc atagtgtccg actcgtcaag gcctgtgcgg gtgagggcgc gcacggactt ctggtggtta ccccttacta ctcgaagccg ccgcagaccg ggctgtttgc gcacttcacc gctgtggccg acgcgactga gctaccagtg ttgctctacg acattcccgg gcggtcggtc gtgccgatcg agcctgacac gattcgcgcg ctggcgtcgc atcccaacat cgtcggagtc aaagaggcca aggctgattt atacagcggt gcccggatca tggctgacac cggcctggcc tactattccg gcgacgacgc actgaacctg ccctggctgg cggtgggtgc catcggcttc atcagtgtga tttctcatct agccgcagga cagcttcgag agctgttatc cgcttttggt tctggggata ttaccactgc ccgaaagatc aacgtcgcga tcggcccgct gtgcagcgcg atggaccgct tgggtggggt gacgatgtcc aaggcaggtc tgcggcttca gggtatcgac gtcggtgatc cgcggttgcc gcagatgccg gcaacagcgg agcagatcga tgagttggct gtcgatatgc gtgcagcctc ggtgcttagg tga
Details for MyleA.01563.a.A1.GE34136
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTTVGFDV PARLGTLLTA MVTPFDADGS VDTAAATRLA NRLVDAGCDG LVLSGTTGES PTTTDDEKLQ LLRVVLEAVG DRARVIAGAG SYDTAHSVRL VKACAGEGAH GLLVVTPYYS KPPQTGLFAH FTAVADATEL PVLLYDIPGR SVVPIEPDTI RALASHPNIV GVKEAKADLY SGARIMADTG LAYYSGDDAL NLPWLAVGAI GFISVISHLA AXQLRELLSA FGSGDITTAR KINVAIXPLC SAMDRLGGVT MSKAGLRLXG IDVXDPRLPQ MPAXAXXIDE LXXDXRAXRA XXXXSTXVLX XLXXXAANKX XXX
Validated NT Sequence
ttttnnttnn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ccactgtcgg attcgacgtc cccgcacgtt tggggaccct gcttactgcg atggtgacac cgtttgacgc tgatggttct gttgacactg cggctgcgac gcggctggcg aaccgcctgg tcgacgcggg ttgtgatggt ctggtgctct cgggcaccac cggcgagtcg ccgaccacta ctgacgacga gaaactccaa ctgttgcgtg tcgtacttga ggcggtaggt gaccgagcta gagtcatcgc cggcgcaggt agttatgaca cagctcatag tgtccgactc gtcaaggcct gtgcgggtga gggcgcgcac ggacttctgg tggttacccc ttactactcg aagccgccgc agaccgggct gtttgcgcac ttcaccgctg tggccgacgc gactgagcta ccagtgttgc tctacgacat tcccgggcgg tcggtcgtgc cgatcgagcc tgacacgatt cgcgcgctgg cgtcgcatcc caacatcgtc ggagtcaaag aggccaaggc tgatttatac agcggtgccc ggatcatggc tgacaccggc ctggcctact attccggcga cgacgcactg aacctgccct ggctggcggt gggtgccatc ggcttcatca gtgtgatttc tcatctagcc gcangacagc ttcgagagct gttatccgct tttggttctg gggatattac cactgcccga aagatcaacg tcgcgatcng cccgctgtgc agcgcgatgg accgcttggg tggggtgacn atgtccaagg caggtctgcg gcttcngggt atcgacgtcg nngatccncg gttgccgcag atgccggcan cagcngancn natcgatgag ttggntgncg atntgcgtgc annncgngct tangnannna ncagcacnan agtnctgcan nnnctnnnng nnncngctgc taacaagncn naannnanct gantnntgct gcnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTTVGFDV PARLGTLLTA MVTPFDADGS VDTAAATRLA NRLVDAGCDG LVLSGTTGES PTTTDDEKLQ LLRVVLEAVG DRARVIAGAG SYDTAHSVRL VKACAGEGAH GLLVVTPYYS KPPQTGLFAH FTAVADATEL PVLLYDIPGR SVVPIEPDTI RALASHPNIV GVKEAKADLY SGARIMADTG LAYYSGDDAL NLPWLAVGAI GFISVISHLA AGQLRELLSA FGSGDITTAR KINVAIGPLC SAMDRLGGVT MSKAGLRLQG IDVGDPRLPQ MPATAEQIDE LAVDMRAASV LR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaccact gtcggattcg acgtccccgc acgtttgggg accctgctta ctgcgatggt gacaccgttt gacgctgatg gttctgttga cactgcggct gcgacgcggc tggcgaaccg cctggtcgac gcgggttgtg atggtctggt gctctcgggc accaccggcg agtcgccgac cactactgac gacgagaaac tccaactgtt gcgtgtcgta cttgaggcgg taggtgaccg agctagagtc atcgccggcg caggtagtta tgacacagct catagtgtcc gactcgtcaa ggcctgtgcg ggtgagggcg cgcacggact tctggtggtt accccttact actcgaagcc gccgcagacc gggctgtttg cgcacttcac cgctgtggcc gacgcgactg agctaccagt gttgctctac gacattcccg ggcggtcggt cgtgccgatc gagcctgaca cgattcgcgc gctggcgtcg catcccaaca tcgtcggagt caaagaggcc aaggctgatt tatacagcgg tgcccggatc atggctgaca ccggcctggc ctactattcc ggcgacgacg cactgaacct gccctggctg gcggtgggtg ccatcggctt catcagtgtg atttctcatc tagccgcagg acagcttcga gagctgttat ccgcttttgg ttctggggat attaccactg cccgaaagat caacgtcgcg atcggcccgc tgtgcagcgc gatggaccgc ttgggtgggg tgacgatgtc caaggcaggt ctgcggcttc agggtatcga cgtcggtgat ccgcggttgc cgcagatgcc ggcaacagcg gagcagatcg atgagttggc tgtcgatatg cgtgcagcct cggtgcttag gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa