MyleA.01548.a

Methionine aminopeptidase

CENTER ID:	MyleA.01548.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01548.a.A1.GE29269	Full length( MyleA.01548.a )		1	266

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126721
PATRIC ID:	fig\|561304.4.peg.3492
RefSeq:	YP_002503871.1
UniProt:	A0A0H3MR81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGALTRLRSR RVVPQRSVGE LDAMAAAGAV VAAALHAVRD AAGPGVSSLS LDEIAESVIR EAGATPSFLR YHGYPATICT SVNDRVIHGI PSAVEILAPG DLVSIDCGAV LDGWHGDAAI TFGVGTLTSV DEALSQATRE SLEAGIAAMI VNNRLTDVAH AIEVSTRTSE IRYRLTFGIV QGYGGHGIGR HMHMDPFLPN EGAPGRGPLL VPGSVLAIEP MLTLGTGKTI VLDDEWTVTT ADGSRAAHWE HTVAVTEDGP RVLTLA

NT Sequence

GTGGGCGCGT TGACACGGCT GCGCAGTCGC AGGGTTGTGC CGCAGCGCAG TGTTGGCGAA CTTGACGCGA TGGCCGCAGC CGGTGCAGTG GTCGCGGCAG CACTGCACGC CGTCCGGGAC GCTGCAGGCC CCGGGGTGTC GAGTCTCAGC CTCGACGAAA TTGCCGAGTC AGTGATCCGC GAAGCCGGAG CTACACCGTC ATTTCTCCGT TATCACGGCT ACCCCGCTAC CATCTGCACA TCGGTTAATG ACCGGGTGAT TCATGGAATC CCCTCTGCCG TTGAGATTCT CGCACCCGGC GACCTCGTGT CGATCGACTG CGGCGCGGTG CTGGACGGCT GGCACGGTGA TGCGGCGATA ACCTTTGGTG TGGGCACCCT CACTTCCGTG GATGAAGCCT TGTCCCAGGC GACCAGAGAA TCGCTGGAAG CCGGCATTGC GGCGATGATT GTCAACAATC GTTTGACCGA CGTGGCGCAT GCCATCGAAG TGAGTACCCG TACGTCCGAG ATTCGATACC GACTTACGTT TGGGATCGTG CAAGGTTACG GGGGTCATGG CATCGGCCGG CACATGCACA TGGATCCGTT TCTGCCCAAC GAAGGCGCTC CTGGGCGCGG CCCGCTGCTG GTACCTGGCT CGGTACTGGC CATCGAACCG ATGTTGACGC TTGGCACCGG CAAAACGATT GTGCTCGACG ACGAATGGAC GGTAACGACT GCTGACGGTT CGCGTGCGGC TCATTGGGAG CACACTGTGG CCGTCACCGA GGACGGACCT CGCGTTCTTA CTCTGGCC

Details for MyleA.01548.a.A1.GE29269

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVGALTRLR SRRVVPQRSV GELDAMAAAG AVVAAALHAV RDAAGPGVSS LSLDEIAESV IREAGATPSF LRYHGYPATI CTSVNDRVIH GIPSAVEILA PGDLVSIDCG AVLDGWHGDA AITFGVGTLT SVDEALSQAT RESLEAGIAA MIVNNRLTDV AHAIEVSTRT SEIRYRLTFG IVQGYGGHGI GRHMHMDPFL PNEGAPGRGP LLVPGSVLAI EPMLTLGTGK TIVLDDEWTV TTADGSRAAH WEHTVAVTED GPRVLTLA

Validated NT Sequence

ttattaggcc agagtaagaa cgcgaggtcc gtcctcggtg acggccacag tgtgctccca atgagccgca cgcgaaccgt cagcagtcgt taccgtccat tcgtcgtcga gcacaatcgt tttgccggtg ccaagcgtca acatcggttc gatggccagt accgagccag gtaccagcag cgggccgcgc ccaggagcgc cttcgttggg cagaaacgga tccatgtgca tgtgccggcc gatgccatga cccccgtaac cttgcacgat cccaaacgta agtcggtatc gaatctcgga cgtacgggta ctcacttcga tggcatgcgc cacgtcggtc aaacgattgt tgacaatcat cgccgcaatg ccggcttcca gcgattctct ggtcgcctgg gacaaggctt catccacgga agtgagggtg cccacaccaa aggttatcgc cgcatcaccg tgccagccgt ccagcaccgc gccgcagtcg atcgacacga ggtcgccggg tgcgagaatc tcaacggcag aggggattcc atgaatcacc cggtcattaa ccgatgtgca gatggtagcg gggtagccgt gataacggag aaatgacggt gtagctccgg cttcgcggat cactgactcg gcaatttcgt cgaggctgag actcgacacc ccggggcctg cagcgtcccg gacggcgtgc agtgctgccg cgaccactgc accggctgcg gccatcgcgt caagttcgcc aacactgcgc tgcggcacaa ccctgcgact gcgcagccgt gtcaacgcgc ccaccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgggcgcg ttgacacggc tgcgcagtcg cagggttgtg ccgcagcgca gtgttggcga acttgacgcg atggccgcag ccggtgcagt ggtcgcggca gcactgcacg ccgtccggga cgctgcaggc cccggggtgt cgagtctcag cctcgacgaa attgccgagt cagtgatccg cgaagccgga gctacaccgt catttctccg ttatcacggc taccccgcta ccatctgcac atcggttaat gaccgggtga ttcatggaat cccctctgcc gttgagattc tcgcacccgg cgacctcgtg tcgatcgact gcggcgcggt gctggacggc tggcacggtg atgcggcgat aacctttggt gtgggcaccc tcacttccgt ggatgaagcc ttgtcccagg cgaccagaga atcgctggaa gccggcattg cggcgatgat tgtcaacaat cgtttgaccg acgtggcgca tgccatcgaa gtgagtaccc gtacgtccga gattcgatac cgacttacgt ttgggatcgt gcaaggttac gggggtcatg gcatcggccg gcacatgcac atggatccgt ttctgcccaa cgaaggcgct cctgggcgcg gcccgctgct ggtacctggc tcggtactgg ccatcgaacc gatgttgacg cttggcaccg gcaaaacgat tgtgctcgac gacgaatgga cggtaacgac tgctgacggt tcgcgtgcgg ctcattggga gcacactgtg gccgtcaccg aggacggacc tcgcgttctt actctggcca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a