SSGCID: Target Page

MyleA.01494.a

POSSIBLE AMINOTRANSFERASE

CENTER ID:	MyleA.01494.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01494.a.A1.GE34135	full length		1	398

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
RefSeq:	WP_010907538.1
RefSeq:	YP_002502844.1
UniProt:	A0A0H3MP90

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAYDAARVRG LHPSLGDGWV HFDAPAGMLI PDSVATTVST AFRRSSSTTS GAHPSAQRSA AVLAAARASV ADLFGADPAG VVLAADRAVL LSSLAAASSS CAGLGYEVIV SRLDDEVNIA PWLRAAHRHG AKVKWAEVDI ETGELPTWQW ESLIGESTRL VAVTSASGTL GTVTDLRAMT KLVHDVGGLV VVDHSAASPY RLLDLNETEV DVVAVNAHAW GGPPIGAVVF RDPGLIDTFT LASTDPHAAG PARLEVGLHQ FGLLAGVVDS IEYLAALDES ARGSRRERLS VSMQSAGLYL NRIFDYLMMS LRSLSLVMVI GRPEVRIPVV SFVHQGVPAD MVVQRLADNG ILAICDPRSR VLDVLGVNDI GGAVTVGLAH YSTTVEVDQL VRTLASLG

NT Sequence

atggcatatg acgccgcccg ggtgcgcggt ctgcacccgt ccctgggtga tggctgggtg catttcgatg cgccggctgg gatgctgatt ccagattccg tcgcgaccac agtgtcgact gcattccgca gatccagctc caccacatca ggtgctcacc cgtccgcgca gcgcagtgcc gcggtgctgg ccgcggcgcg tgcctcggtg gccgatttgt tcggcgcgga cccagctggt gtcgtgttag ccgccgaccg cgcggtctta ttgtcatcgt tggccgcagc gtcgtcctcg tgtgccggcc tgggatacga ggtgattgtc agccgcctcg acgacgaggt gaatatagct ccgtggctgc gggcggcaca ccgccacggt gcaaaggtga aatgggccga ggtcgatatc gagaccggtg agttgccgac gtggcagtgg gagagcctga tcggcgaatc gacccgactg gtggccgtta cgtcagcgtc agggacgttg ggtaccgtta ccgacctgcg ggcgatgact aagctggttc acgatgttgg cgggcttgtc gtagttgatc attccgccgc ctccccctac cggctgttag atctcaacga gaccgaggtt gacgtggtgg cggtgaacgc tcatgcatgg ggcggtccgc cgatcggggc tgtggtgttt cgcgaccccg ggttgatcga caccttcacc ttagcgtcga cagaccccca cgccgcgggc ccggcgcgtc tggaggttgg tttgcatcag ttcggactgc tggccggtgt agtggacagc atagaatacc ttgctgcact tgatgaatcg gctcgtggca gcaggcgcga acgattatcg gtgtcaatgc aatccgccgg tttgtatctg aaccggatat tcgactacct gatgatgtcg ttgcgatctt tgtcgttggt gatggtgatc ggccgtccgg aggtgcggat accggtcgtc agttttgtgc accaaggggt tcctgcagac atggtggtgc agcgcctggc tgacaacgga attctggcga tatgtgaccc aaggtcacgt gtgcttgacg tactcggcgt aaatgacatc ggcggcgcgg ttactgtcgg gctagcgcat tattcgacga cggtcgaagt cgaccagtta gtgcgcacgc tggcgtcgct tggctag

Details for MyleA.01494.a.A1.GE34135

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	71

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAYDAARVR GLHPSLGDGW VHFDAPAGML IPDSVATTVS TAFRRSSSTT SGAHPSAQRS AAVLAAARAS VADLFGADPA GVVLAADRAV LLSSLAAASS SCAGLGYEVI VSRLDDEVNI APWLRAAHRH GAKVKWAEVD IETGELPTWQ WESLIGESTR LVAVTSASGT LGTVTDLRAM TKLVHDVGGL VVVDHSAASP YRLLDLNETE VDVVAVNAHA WGGPPIGAVV FRDPGLIDTF TLASTDPHAA GPARLEVGLH QFXLLXRCSG QHRIPCCT

Validated NT Sequence

tattttnttt aactttangn nggagatata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggc atatgacgcc gcccgggtgc gcggtctgca cccgtccctg ggtgatggct gggtgcattt cgatgcgccg gctgggatgc tgattccaga ttccgtcgcg accacagtgt cgactgcatt ccgcagatcc agctccacca catcaggtgc tcacccgtcc gcgcagcgca gtgccgcggt gctggccgcg gcgcgtgcct cggtggccga tttgttcggc gcggacccag ctggtgtcgt gttagccgcc gaccgcgcgg tcttattgtc atcgttggcc gcagcgtcgt cctcgtgtgc cggcctggga tacgaggtga ttgtcagccg cctcgacgac gaggtgaata tagctccgtg gctgcgggcg gcacaccgcc acggtgcaaa ggtgaaatgg gccgaggtcg atatcgagac cggtgagttg ccgacgtggc agtgggagag cctgatcggc gaatcgaccc gactggtggc cgttacgtca gcgtcaggga cgttgggtac cgttaccgac ctgcgggcga tgactaagct ggttcacgat gttggcgggc ttgtcgtagt tgatcattcc gccgcctccc cctaccggct gttagatctc aacgagaccg aggttgacgt ggtggcggtg aacgctcatg catggggcgg tccgccgatc ggggctgtgg tgtttcgcga ccccgggttg atcgacacct tcaccttagc gtcgacagac ccccacgccg cgggcccggc gcgtctggag gttggtttgc atcagttcng actgctngnc cggtgtagtg gacagcatag aataccttgc tgcacttgan gaatcngctc gtggcancag gnnngaacga tnatcggtgt cnatgcaatn nttgnatctg nnnnnnntnn actnctgang atgncntncn atcttnnctg ngangngatn nnnnnnnnnn nnnnnnnnng tcantttnng nnncnngtnn gcnnannngn nggnnnnnnn cngnnganna cnnnnnnnnn gnntnnnnnn nnnnnnnnnn nnnnntnnnn nnnnnnnnnn nanc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcatatgac gccgcccggg tgcgcggtct gcacccgtcc ctgggtgatg gctgggtgca tttcgatgcg ccggctggga tgctgattcc agattccgtc gcgaccacag tgtcgactgc attccgcaga tccagctcca ccacatcagg tgctcacccg tccgcgcagc gcagtgccgc ggtgctggcc gcggcgcgtg cctcggtggc cgatttgttc ggcgcggacc cagctggtgt cgtgttagcc gccgaccgcg cggtcttatt gtcatcgttg gccgcagcgt cgtcctcgtg tgccggcctg ggatacgagg tgattgtcag ccgcctcgac gacgaggtga atatagctcc gtggctgcgg gcggcacacc gccacggtgc aaaggtgaaa tgggccgagg tcgatatcga gaccggtgag ttgccgacgt ggcagtggga gagcctgatc ggcgaatcga cccgactggt ggccgttacg tcagcgtcag ggacgttggg taccgttacc gacctgcggg cgatgactaa gctggttcac gatgttggcg ggcttgtcgt agttgatcat tccgccgcct ccccctaccg gctgttagat ctcaacgaga ccgaggttga cgtggtggcg gtgaacgctc atgcatgggg cggtccgccg atcggggctg tggtgtttcg cgaccccggg ttgatcgaca ccttcacctt agcgtcgaca gacccccacg ccgcgggccc ggcgcgtctg gaggttggtt tgcatcagtt cggactgctg gccggtgtag tggacagcat agaatacctt gctgcacttg atgaatcggc tcgtggcagc aggcgcgaac gattatcggt gtcaatgcaa tccgccggtt tgtatctgaa ccggatattc gactacctga tgatgtcgtt gcgatctttg tcgttggtga tggtgatcgg ccgtccggag gtgcggatac cggtcgtcag ttttgtgcac caaggggttc ctgcagacat ggtggtgcag cgcctggctg acaacggaat tctggcgata tgtgacccaa ggtcacgtgt gcttgacgta ctcggcgtaa atgacatcgg cggcgcggtt actgtcgggc tagcgcatta ttcgacgacg gtcgaagtcg accagttagt gcgcacgctg gcgtcgcttg gctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa