MyleA.01340.a

DIHYDROLIPOAMIDE DEHYDROGENASE LPD (LIPOAMIDE REDUCTASE (NADH)) (LIPOYL DEHYDROGENASE) (DIHYDROLIPOYL DEHYDROGENASE) (DIAPHORASE

CENTER ID: MyleA.01340.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01340.a.A1.GE34132 full length 1 467
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4613
RefSeq: YP_002504174.1
UniProt: A0A0H3MWF6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHYDVVVLG AGPGGYVAAI RAAQLGLSTA VVEPKYWGGI CLNVGCIPSK VLLHNAELAH IFTKEAKTFG ISGDASFDYG IAYDRSRKVS EGRVAGVHFL MKKNKITEIH GYGRFTDANT LSVELSEGVP ETPLKVTFNN VIIATGSKTR LVPGTLLSTN VITYEEQILT RELPDSIVIV GAGAIGIEFG YVLKNYGVDV TIVEFLPRAM PNEDAEVSKE IEKQFKKMGI KILTGTKVES ISDNGSHVLV AVSKDGQFQE LKADKVLQAI GFAPNVDGYG LDKVGVALTA DKAVDIDDYM QTNVSHIYAI GDVTGKLQLA HVAEAQGVVA AEAIAGAETL ALSDYRMMPR ATFCQPNVAS FGLTEQQARD GGYDVVVAKF PFTANAKAHG MGDPSGFVKL VADAKYGELL GGHMIGHNVS ELLPELTLAQ KWDLTATELV RNVHTHPTLS EALQECFHGL IGHMINF
NT Sequence
gtgactcact atgatgtcgt tgtgctcgga gccggtccgg gcggatatgt cgctgccatt cgtgctgcac agctcggctt gagcacagca gtcgtcgaac ccaagtactg gggagggatc tgcctcaacg tcggctgcat cccgtccaag gtgttattgc acaacgccga actcgcacac atcttcacta aagaggcgaa gactttcggc atcagcggcg atgccagctt cgactacggg attgcctacg accgtagccg caaggtgtcc gagggtcgcg tcgccggcgt gcacttcctg atgaagaaaa acaagatcac cgagatacac ggctacggca gattcaccga cgccaacacg ttgtccgttg aattgtcgga aggcgtccct gaaactccgc taaaggtcac gttcaacaac gtcatcatcg ccaccggcag taaaacccgg ctggtgcctg gcacgttgct gtcgaccaac gtgattacct acgaggagca gatcctgacc cgggagcttc ccgactcgat cgtcatcgtc ggtgcagggg ccatcggtat agaattcggc tacgtcctga agaactacgg tgtcgacgtg acgatcgtgg aattcctgcc gcgcgcgatg cccaacgagg acgccgaagt gtctaaggag atcgagaagc aattcaagaa gatgggcatc aaaatcctta ccggaaccaa agtggagtcc atctctgaca acggttcgca tgtcttggtg gctgtcagca aggacggaca attccaggaa ctcaaggccg acaaagtgtt gcaggccatc gggtttgctc ccaacgtcga cggttacgga ctggacaagg tcggggtagc tttgaccgcc gacaaggcgg tcgacatcga tgactatatg caaaccaatg tttcgcacat ctatgccatc ggcgatgtta ccggtaagct gcagctggcc catgttgccg aagcccaagg ggtggttgcg gccgaggcta tcgccggcgc agagactttg gcgctcagtg actaccgtat gatgccacgc gcaacgttct gccagccgaa tgtggccagc ttcgggctga ccgagcagca agcccgagac gggggttatg acgtcgtagt cgctaagttc ccgtttactg ccaacgcgaa agcgcacggc atgggtgacc ccagcggttt cgtcaagctc gttgcggacg ccaagtatgg ggagctgttg ggcgggcaca tgatcggcca taacgtgtcc gagttactac cggagctcac gctcgcgcag aaatgggatt tgacggccac cgagctggtt cgcaacgtgc atacgcatcc gacgctgtca gaggcgctgc aggagtgctt tcacggccta atcggccata tgatcaactt ctga
Details for MyleA.01340.a.A1.GE34132
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 52kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 77
PERCENT COVERAGE: 79
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTHYDVVV LGAGPGGYVA AIRAAQLGLS TAVVEPKYWG GICLNVGCIP SKVLLHNAEL AHIFTKEAKT FGISGDASFD YGIAYDRSRK VSEGRVAGVH FLMKKNKITE IHGYGRFTDA NTLSVELSEG VPETPLKVTF NNVIIATGSK TRLVPGTLLS TNVITYEEQI LTRELPDSIV IVGAGAIGIE FGYVLKNYGV DVTIVEFLPR AMPNEDAEVS XEIEKQFKKM GIKILTGTKV ESISDNGSHV LXAVSKDGQF QELKADKVLX XSGLLPTSXX XXWTXRGSFD XXXXTSMTXC XXXFXHLCHX XVTXXCXXXX XXXPXGXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
tttnttnnnt ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggtgact cactatgatg tcgttgtgct cggagccggt ccgggcggat atgtcgctgc cattcgtgct gcacagctcg gcttgagcac agcagtcgtc gaacccaagt actggggagg gatctgcctc aacgtcggct gcatcccgtc caaggtgtta ttgcacaacg ccgaactcgc acacatcttc actaaagagg cgaagacttt cggcatcagc ggcgatgcca gcttcgacta cgggattgcc tacgaccgta gccgcaaggt gtccgagggt cgcgtcgccg gcgtgcactt cctgatgaag aaaaacaaga tcaccgagat acacggctac ggcagattca ccgacgccaa cacgttgtcc gttgaattgt cggaaggcgt ccctgaaact ccgctaaagg tcacgttcaa caacgtcatc atcgccaccg gcagtaaaac ccggctggtg cctggcacgt tgctgtcgac caacgtgatt acctacgagg agcagatcct gacccgggag cttcccgact cgatcgtcat cgtcggtgca ggggccatcg gtatagaatt cggctacgtc ctgaagaact acggtgtcga cgtgacgatc gtggaattcc tgccgcgcgc gatgcccaac gaggacgccg aagtgtctaa ngagatcgag aagcaattca agaagatggg catcaaaatc cttaccggaa ccaaagtgga gtccatctct gacaacggtt cgcatgtctt ggnggctgtc agcaaggacg gacaattcca ggaactcaag gccgacaaag tgttgcnnnc atcgggtttg ctcccnacgt cnanggntan gnactggacn nnncggggta gctttgacnc nananngncg acatcgatga ctatntgcna nnnannnttc nnncatctnt gccatngnna ngttaccnna anctgcanct gnnnntnnnt gncnaanccn nnnggnngnn nngnnnnnng nnnnnnnant tnnncncnng nnnnnnnnnn nngnntntnn cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nannnnnnnn nnnnnncnnn nnnnnnnnnn nnnnnnnnnn ng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTHYDVVV LGAGPGGYVA AIRAAQLGLS TAVVEPKYWG GICLNVGCIP SKVLLHNAEL AHIFTKEAKT FGISGDASFD YGIAYDRSRK VSEGRVAGVH FLMKKNKITE IHGYGRFTDA NTLSVELSEG VPETPLKVTF NNVIIATGSK TRLVPGTLLS TNVITYEEQI LTRELPDSIV IVGAGAIGIE FGYVLKNYGV DVTIVEFLPR AMPNEDAEVS KEIEKQFKKM GIKILTGTKV ESISDNGSHV LVAVSKDGQF QELKADKVLQ AIGFAPNVDG YGLDKVGVAL TADKAVDIDD YMQTNVSHIY AIGDVTGKLQ LAHVAEAQGV VAAEAIAGAE TLALSDYRMM PRATFCQPNV ASFGLTEQQA RDGGYDVVVA KFPFTANAKA HGMGDPSGFV KLVADAKYGE LLGGHMIGHN VSELLPELTL AQKWDLTATE LVRNVHTHPT LSEALQECFH GLIGHMINF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactcac tatgatgtcg ttgtgctcgg agccggtccg ggcggatatg tcgctgccat tcgtgctgca cagctcggct tgagcacagc agtcgtcgaa cccaagtact ggggagggat ctgcctcaac gtcggctgca tcccgtccaa ggtgttattg cacaacgccg aactcgcaca catcttcact aaagaggcga agactttcgg catcagcggc gatgccagct tcgactacgg gattgcctac gaccgtagcc gcaaggtgtc cgagggtcgc gtcgccggcg tgcacttcct gatgaagaaa aacaagatca ccgagataca cggctacggc agattcaccg acgccaacac gttgtccgtt gaattgtcgg aaggcgtccc tgaaactccg ctaaaggtca cgttcaacaa cgtcatcatc gccaccggca gtaaaacccg gctggtgcct ggcacgttgc tgtcgaccaa cgtgattacc tacgaggagc agatcctgac ccgggagctt cccgactcga tcgtcatcgt cggtgcaggg gccatcggta tagaattcgg ctacgtcctg aagaactacg gtgtcgacgt gacgatcgtg gaattcctgc cgcgcgcgat gcccaacgag gacgccgaag tgtctaagga gatcgagaag caattcaaga agatgggcat caaaatcctt accggaacca aagtggagtc catctctgac aacggttcgc atgtcttggt ggctgtcagc aaggacggac aattccagga actcaaggcc gacaaagtgt tgcaggccat cgggtttgct cccaacgtcg acggttacgg actggacaag gtcggggtag ctttgaccgc cgacaaggcg gtcgacatcg atgactatat gcaaaccaat gtttcgcaca tctatgccat cggcgatgtt accggtaagc tgcagctggc ccatgttgcc gaagcccaag gggtggttgc ggccgaggct atcgccggcg cagagacttt ggcgctcagt gactaccgta tgatgccacg cgcaacgttc tgccagccga atgtggccag cttcgggctg accgagcagc aagcccgaga cgggggttat gacgtcgtag tcgctaagtt cccgtttact gccaacgcga aagcgcacgg catgggtgac cccagcggtt tcgtcaagct cgttgcggac gccaagtatg gggagctgtt gggcgggcac atgatcggcc ataacgtgtc cgagttacta ccggagctca cgctcgcgca gaaatgggat ttgacggcca ccgagctggt tcgcaacgtg catacgcatc cgacgctgtc agaggcgctg caggagtgct ttcacggcct aatcggccat atgatcaact tctaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa