SSGCID: Target Page

MyleA.01250.a

Inorganic polyphosphate/ATP-NAD kinase ppnK (Poly(P)/ATP NAD kinase)

CENTER ID:	MyleA.01250.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01250.a.A1.GE34131	full length		1	311

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01250.a.A1.PS01422	full length		1	311

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2520
RefSeq:	YP_002503599.1
UniProt:	B8ZRH2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTPRKAAQRT VLLVVHTGRD EATETARRVK KIVGDNGIAL RVLSAEAVDR GSLHLALDNM RAMGVDIEVV DADPHVAQGC ELVLVLGGDG TFLRAAELAR TARIPVLGVN LGRIGFLAEA EAEAIDVVLE HVIARSYRVE ERLTLDIVVR QGGNIIDQGW ALNEASLEKG PRLGVLGVVV EIEGRPVSTF GCDGVLVSTP TGSTAYAFSA GGPVLWPDLE AILVVPNNAH ALFGRPMVTS PDATVAIELE ANGNDALVFC DGRREMIIPA GGRLEVTRCA TPVKWARLDS APFTDRLVSK FRLPVTGWRG K

NT Sequence

gtgactcctc gcaaggcggc gcaacggact gtgttgctgg tcgtccacac cggacgtgac gaagcaaccg agacggctcg tcgtgtaaag aaaattgtgg gcgacaatgg aattgcactt cgggtgttat ctgctgaggc ggtcgatagg ggatcgctgc atcttgctct cgacaatatg cgtgcaatgg gtgttgatat cgaggtggtt gacgctgacc cgcacgttgc ccagggttgc gaactggtgc tggtgctagg tggtgatggc accttcttac gtgccgctga gctggctcgc accgcccgca tcccagtgtt gggtgtcaac ctgggacgga tcggcttttt agcggaggcc gaagcggagg ctattgacgt tgtgctggag cacgtcatcg cgcggagcta ccgcgtggag gagcgcttaa cactcgacat cgtggtacgc caaggcggga acatcatcga tcaggggtgg gcacttaacg aagccagctt ggaaaagggt ccgcggctgg gtgtgctcgg ggtggtcgtc gaaatcgaag ggcggccagt gtccacgttc ggttgcgacg gggtgttggt atcgaccccc acgggatcca ccgcctatgc gttctcggca ggtggtccgg tgctatggcc ggatctcgaa gctatcctgg tggtccccaa caacgctcat gctctgttcg gccggccgat ggtcaccagc cccgatgcta ccgtggcaat tgagctagag gccaacggca acgacgcact ggtgttctgc gacggtcgcc gcgaaatgat tataccggcc ggcggacggc tcgaggtgac tcgctgtgcc acaccagtga aatgggcgcg cctagatagc gcgccgttca ctgaccgctt ggtgagcaaa ttccggttgc cggtgaccgg ttggcgcggg aagtga

Details for MyleA.01250.a.A1.GE34131

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	35kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVTPRKAAQ RTVLLVVHTG RDEATETARR VKKIVGDNGI ALRVLSAEAV DRGSLHLALD NMRAMGVDIE VVDADPHVAQ GCELVLVLGG DGTFLRAAEL ARTARIPVLG VNLGRIGFLA EAEAEAIDVV LEHVIARSYR VEERLTLDIV VRQGGNIIDQ GWALNEASLE KGPRLGVLGV VVEIEGRPVS TFGCDGVLVS TPTGSTAYAF SAGGPVLWPD LEAILVVPNN AHALFGRPMV TSPDATVAIE LEANGNDALV FCDGRREMII PAGGRLEVTR CATPVKWARL DSAPFTDRLV SKFRLPVTGW RGK

Validated NT Sequence

ttattacttc ccgcgccaac cggtcaccgg caaccggaat ttgctcacca agcggtcagt gaacggcgcg ctatctaggc gcgcccattt cactggtgtg gcacagcgag tcacctcgag ccgtccgccg gccggtataa tcatttcgcg gcgaccgtcg cagaacacca gtgcgtcgtt gccgttggcc tctagctcaa ttgccacggt agcatcgggg ctggtgacca tcggccggcc gaacagagca tgagcgttgt tggggaccac caggatagct tcgagatccg gccatagcac cggaccacct gccgagaacg cataggcggt ggatcccgtg ggggtcgata ccaacacccc gtcgcaaccg aacgtggaca ctggccgccc ttcgatttcg acgaccaccc cgagcacacc cagccgcgga cccttttcca agctggcttc gttaagtgcc cacccctgat cgatgatgtt cccgccttgg cgtaccacga tgtcgagtgt taagcgctcc tccacgcggt agctccgcgc gatgacgtgc tccagcacaa cgtcaatagc ctccgcttcg gcctccgcta aaaagccgat ccgtcccagg ttgacaccca acactgggat gcgggcggtg cgagccagct cagcggcacg taagaaggtg ccatcaccac ctagcaccag caccagttcg caaccctggg caacgtgcgg gtcagcgtca accacctcga tatcaacacc cattgcacgc atattgtcga gagcaagatg cagcgatccc ctatcgaccg cctcagcaga taacacccga agtgcaattc cattgtcgcc cacaattttc tttacacgac gagccgtctc ggttgcttcg tcacgtccgg tgtggacgac cagcaacaca gtccgttgcg ccgccttgcg aggagtcacc atcgaaccag gaccctgggt ctgagcttcc agggtaccca tatgatggtg atggtgatga gccatggta

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactcct cgcaaggcgg cgcaacggac tgtgttgctg gtcgtccaca ccggacgtga cgaagcaacc gagacggctc gtcgtgtaaa gaaaattgtg ggcgacaatg gaattgcact tcgggtgtta tctgctgagg cggtcgatag gggatcgctg catcttgctc tcgacaatat gcgtgcaatg ggtgttgata tcgaggtggt tgacgctgac ccgcacgttg cccagggttg cgaactggtg ctggtgctag gtggtgatgg caccttctta cgtgccgctg agctggctcg caccgcccgc atcccagtgt tgggtgtcaa cctgggacgg atcggctttt tagcggaggc cgaagcggag gctattgacg ttgtgctgga gcacgtcatc gcgcggagct accgcgtgga ggagcgctta acactcgaca tcgtggtacg ccaaggcggg aacatcatcg atcaggggtg ggcacttaac gaagccagct tggaaaaggg tccgcggctg ggtgtgctcg gggtggtcgt cgaaatcgaa gggcggccag tgtccacgtt cggttgcgac ggggtgttgg tatcgacccc cacgggatcc accgcctatg cgttctcggc aggtggtccg gtgctatggc cggatctcga agctatcctg gtggtcccca acaacgctca tgctctgttc ggccggccga tggtcaccag ccccgatgct accgtggcaa ttgagctaga ggccaacggc aacgacgcac tggtgttctg cgacggtcgc cgcgaaatga ttataccggc cggcggacgg ctcgaggtga ctcgctgtgc cacaccagtg aaatgggcgc gcctagatag cgcgccgttc actgaccgct tggtgagcaa attccggttg ccggtgaccg gttggcgcgg gaagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for MyleA.01250.a.A1.PS01422

PURIFICATION DATe:	3/16/2012
CONCENTRATION:	25.26mg/ml
OBSERVED MW:	36kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGACTCCT CGCAAGGCGG CGCAACGGAC TGTGTTGCTG GTCGTCCACA CCGGACGTGA CGAAGCAACC GAGACGGCTC GTCGTGTAAA GAAAATTGTG GGCGACAATG GAATTGCACT TCGGGTGTTA TCTGCTGAGG CGGTCGATAG GGGATCGCTG CATCTTGCTC TCGACAATAT GCGTGCAATG GGTGTTGATA TCGAGGTGGT TGACGCTGAC CCGCACGTTG CCCAGGGTTG CGAACTGGTG CTGGTGCTAG GTGGTGATGG CACCTTCTTA CGTGCCGCTG AGCTGGCTCG CACCGCCCGC ATCCCAGTGT TGGGTGTCAA CCTGGGACGG ATCGGCTTTT TAGCGGAGGC CGAAGCGGAG GCTATTGACG TTGTGCTGGA GCACGTCATC GCGCGGAGCT ACCGCGTGGA GGAGCGCTTA ACACTCGACA TCGTGGTACG CCAAGGCGGG AACATCATCG ATCAGGGGTG GGCACTTAAC GAAGCCAGCT TGGAAAAGGG TCCGCGGCTG GGTGTGCTCG GGGTGGTCGT CGAAATCGAA GGGCGGCCAG TGTCCACGTT CGGTTGCGAC GGGGTGTTGG TATCGACCCC CACGGGATCC ACCGCCTATG CGTTCTCGGC AGGTGGTCCG GTGCTATGGC CGGATCTCGA AGCTATCCTG GTGGTCCCCA ACAACGCTCA TGCTCTGTTC GGCCGGCCGA TGGTCACCAG CCCCGATGCT ACCGTGGCAA TTGAGCTAGA GGCCAACGGC AACGACGCAC TGGTGTTCTG CGACGGTCGC CGCGAAATGA TTATACCGGC CGGCGGACGG CTCGAGGTGA CTCGCTGTGC CACACCAGTG AAATGGGCGC GCCTAGATAG CGCGCCGTTC ACTGACCGCT TGGTGAGCAA ATTCCGGTTG CCGGTGACCG GTTGGCGCGG GAAGTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA