SSGCID: Target Page

MyleA.01249.a

Putative oxidoreductase

CENTER ID:	MyleA.01249.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01249.a.A1.GE34932	Full length( MyleA.01249.a )		1	304

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.503
RefSeq:	YP_002502974.1
UniProt:	A0A0H3MTZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKWTTADIP DQTGRVAVIT GANTGLGYQT ALALAEHGAH VVLAVRNLDK GKDAAARITA TSAQNNVALQ ELDLASLESV RAAAKQLRSD YDHIDLLINN AGVMWTPKST TKDGFELQFG TNHLGHFAFT GLLLDRLLPI VGSRVITVSS LSHRLFADIH FNDLQWECNY NRVAAYGQSK LANLLFTYEL QRRLATRQTT IAVAAHPGGS RTELTRTLPA LIAPIFSVAE LFLTQDAATG ALPTLRAATD AAVLGGQYFG PDGFAEIRGH PKVVASNGKS HDVDRQLRLW AVSEELTGVV YPVG

NT Sequence

ATGGCCAAGT GGACCACCGC GGACATTCCC GACCAGACGG GTCGTGTCGC CGTCATTACC GGCGCCAACA CCGGCCTCGG CTATCAGACC GCGCTGGCAC TCGCCGAGCA TGGTGCCCAT GTGGTGTTGG CGGTGCGCAA TCTCGACAAG GGCAAGGACG CTGCCGCACG CATCACGGCC ACTAGCGCAC AGAACAACGT TGCGCTGCAA GAGCTCGACT TAGCGTCGTT GGAGTCTGTG CGCGCCGCAG CAAAGCAACT ACGGTCCGAC TACGACCACA TCGACTTGCT GATCAACAAC GCCGGGGTGA TGTGGACACC GAAGTCGACC ACCAAGGACG GTTTCGAGTT GCAGTTCGGC ACCAACCACT TAGGTCACTT TGCCTTCACC GGCTTGCTGC TGGATCGGCT GCTGCCGATC GTCGGTTCGC GGGTGATAAC TGTCAGCAGC CTCAGTCACC GCCTGTTCGC TGACATTCAT TTCAACGATC TGCAGTGGGA ATGCAACTAC AACCGGGTCG CCGCCTATGG GCAATCCAAG CTAGCCAACC TACTGTTCAC TTACGAACTG CAGCGACGAC TGGCCACCCG ACAAACGACG ATCGCTGTGG CCGCCCATCC CGGTGGCTCA CGCACCGAAC TGACCAGGAC TCTGCCCGCG CTGATTGCGC CCATTTTTTC GGTGGCCGAA CTGTTCCTCA CCCAGGATGC AGCAACCGGC GCACTACCGA CCCTGCGGGC TGCCACCGAT GCGGCAGTGC TCGGCGGGCA GTATTTCGGA CCAGACGGGT TCGCAGAAAT ACGGGGCCAC CCCAAAGTTG TGGCGTCCAA CGGCAAGTCC CACGACGTTG ACCGGCAGCT CCGGCTGTGG GCGGTCTCCG AAGAACTCAC CGGGGTGGTC TATCCGGTCG GC

Details for MyleA.01249.a.A1.GE34932

HARVESTED ON:	4/17/2012
SEQUENCED ON:	4/17/2012
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	88
PERCENT COVERAGE:	112

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAKWTTADI PDQTGRVAVI TGANTGLGYQ TALALAEHGA HVVLAVRNLD KGKDAAARIT ATSAQNNVAL QELDLASLES VRAAAKQLRS DYDHIDLLIN NAGVMWTPKS TTKDGFELQF GTNHLGHFAF TGLLLDRLLP IVGSRVITVS SLSHRLFADI HFNDLQWECN YNRVAAYGQS KLANLLFTYE LQRRLATRQT TIAVAAHPGG SRTELTRTLP ALIAPIFSVA ELFLTQDAAT GALPTLRAAT DAAVLGGQYF XXDGFAEIXA TPKLWRPTAX PRRXPAAPXV GXXXXXSPGX XIXSANKXXX XXXXXXXXXX PXXXXXXXXX XKXXXXXXXX XXXXXX

Validated NT Sequence

anattttnnn nnntttaaga aggaganata ccatggctca tcaccatcac catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggc caagtggacc accgcggaca ttcccgacca gacgggtcgt gtcgccgtca ttaccggcgc caacaccggc ctcggctatc agaccgcgct ggcactcgcc gagcatggtg cccatgtggt gttggcggtg cgcaatctcg acaagggcaa ggacgctgcc gcacgcatca cggccactag cgcacagaac aacgttgcgc tgcaagagct cgacttagcg tcgttggagt ctgtgcgcgc cgcagcaaag caactacggt ccgactacga ccacatcgac ttgctgatca acaacgccgg ggtgatgtgg acaccgaagt cgaccaccaa ggacggtttc gagttgcagt tcggcaccaa ccacttaggt cactttgcct tcaccggctt gctgctggat cggctgctgc cgatcgtcgg ttcgcgggtg ataactgtca gcagcctcag tcaccgcctg ttcgctgaca ttcatttcaa cgatctgcag tgggaatgca actacaaccg ggtcgccgcc tatgggcaat ccaagctagc caacctactg ttcacttacg aactgcagcg acgactggcc acccgacaaa cgacgatcgc tgtggccgcc catcccggtg gctcacgcac cgaactgacc aggactctgc ccgcgctgat tgcgcccatt ttttcggtgg ccgaactgtt cctcacccag gatgcagcaa ccggcgcact accgaccctg cgggctgcca ccgatgcggc agtgctcggc gggcagtatt tcgnancaga cgggttcgca gaaatanngg ccaccccaaa gttgtggcgt ccaacggcan tcccacgacg ttnaccggca gctccnnntg tgggnnggnc tncgaanaan tcnccggggn ggnctatcnn gtcggctaat aaanngcncg annnantnnn nancantnnn nnnnnnnnnn ncccncnnnn nnnnnnnnnn nnnnnnnnnn nnanaaaann annnannnnn nnnnnnnnnn nnnnttnann nnnnnannnn nt

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccaagtgg accaccgcgg acattcccga ccagacgggt cgtgtcgccg tcattaccgg cgccaacacc ggcctcggct atcagaccgc gctggcactc gccgagcatg gtgcccatgt ggtgttggcg gtgcgcaatc tcgacaaggg caaggacgct gccgcacgca tcacggccac tagcgcacag aacaacgttg cgctgcaaga gctcgactta gcgtcgttgg agtctgtgcg cgccgcagca aagcaactac ggtccgacta cgaccacatc gacttgctga tcaacaacgc cggggtgatg tggacaccga agtcgaccac caaggacggt ttcgagttgc agttcggcac caaccactta ggtcactttg ccttcaccgg cttgctgctg gatcggctgc tgccgatcgt cggttcgcgg gtgataactg tcagcagcct cagtcaccgc ctgttcgctg acattcattt caacgatctg cagtgggaat gcaactacaa ccgggtcgcc gcctatgggc aatccaagct agccaaccta ctgttcactt acgaactgca gcgacgactg gccacccgac aaacgacgat cgctgtggcc gcccatcccg gtggctcacg caccgaactg accaggactc tgcccgcgct gattgcgccc attttttcgg tggccgaact gttcctcacc caggatgcag caaccggcgc actaccgacc ctgcgggctg ccaccgatgc ggcagtgctc ggcgggcagt atttcggacc agacgggttc gcagaaatac ggggccaccc caaagttgtg gcgtccaacg gcaagtccca cgacgttgac cggcagctcc ggctgtgggc ggtctccgaa gaactcaccg gggtggtcta tccggtcggc aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa