MyleA.01187.a

PROBABLE GLUTAMYL-TRNA SYNTHETASE GLTS (GLUTAMATE--TRNA LIGASE) (GLUTAMYL-TRNA SYNTHASE) (GLURS)

CENTER ID: MyleA.01187.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01187.a.A1.GE34130 full length 1 502
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3194
RefSeq: YP_002503790.1
UniProt: A0A0H3MR83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVTSDGTP QAAKVRVRFC PSPTGVPHVG MVRTALFNWA YARHTGGTFV LRIEDTDADR DSEESYLALL DALRWLGLNW DEGPEVGGPY GPYRQSQRTD IYREVVAKLL ATGEAYYAFS TPEEVENRHL AAGRNPKLGY DNFDRDLTDA QFSAYLAEGR KPVVRLRMPD EDISWDDLVR GTTTFAVGTV PDYVLTRASG DPLYTLVNPC DDALMKITHV LRGEDLLSST PRQVALYQAL IRIGMAERIP EFGHFPSVLG EGTKKLSKRE PQSNLFAHRD RGFIPEGLLN YLALLGWAIA DDHDLFSLDE MVAAFDVVDV NSNPARFDQK KADAVNAEHI RMLDSEDFAG RLRDYFTTHG YHIALDPANY EAGFVAAAQL VQTRIVVLGD AWDLLKFLND DEYSIDSKAA AKELDADAGP VLDVACAVLD SLVDWTTASI EDVLKVALIE GLGLKPRKVF GPIRVAATGA LVSPPLFESL ELLGRARSLQ RLSAARARVT SA
NT Sequence
gtgactgcag taacctctga tggtaccccg caagcagcaa aagtcagggt acgattctgc ccatcgccca ctggtgttcc gcacgtcggg atggttcgca cggccctgtt caactgggct tacgctcgac atactggagg caccttcgtc ttacgcatcg aggacaccga cgccgaccgc gacagtgaag aaagctacct ggccctgctc gatgcgttgc gctggctcgg cctgaactgg gacgagggtc ccgaggttgg aggaccttac ggtccgtacc ggcagtcgca gcgcactgac atctaccgcg aggtggtggc aaagttgctt gctacaggag aggcctatta cgctttctcc acaccggagg aagtcgagaa ccgccatctt gctgctggac gcaatcccaa gctgggttat gacaactttg accgcgatct taccgatgcg cagttctcag cgtacttggc ggaaggccgt aagcctgtag ttcggctgcg gatgcccgac gaggatatca gttgggatga cctggtgcgc gggacaacta cctttgctgt gggcactgtg cccgattacg tgttgacccg agcaagcgga gatccgttgt acactttggt caacccgtgc gatgacgcgc tgatgaagat cacgcatgta ctgcggggcg aagacttact atcgtcgact ccgcgacagg tggcgctata ccaggcgttg atccgaatcg ggatggctga gcgtattccc gaattcggtc atttcccatc ggtattgggg gagggaacca agaaactctc gaagcgagaa ccgcagtcga atttgtttgc ccaccgtgac cggggcttta tccccgaagg tctgctgaac taccttgcct tgctggggtg ggcgatcgcc gatgaccacg acttgttcag tctcgatgag atggtagccg cgttcgatgt ggtcgacgtc aactccaatc cggcgcgatt cgatcagaag aaggccgatg cggttaacgc cgagcacatc cgaatgctcg actccgagga tttcgctggc aggctacgcg actacttcac cacacacggc taccacatcg ctttggatcc cgctaattac gaagctggtt tcgttgccgc ggcgcagttg gtgcagaccc gcatcgttgt gcttggtgac gcttgggacc tgttgaaatt cctcaacgat gacgagtact ctatcgactc taaggccgct gccaaagaac tggatgccga tgccgggccg gtgcttgatg ttgcctgtgc cgtgctggac agcctggtgg attggaccac tgcgagtatc gaggacgtcc tcaaggttgc actcatcgaa ggtcttggtc ttaaaccgcg caaagtgttt ggcccgatcc gggtcgccgc cacgggggca ttggtcagcc cgccgctgtt cgaatcgctg gagctactcg gccgtgctcg cagtctgcag cggctgagtg ctgcgcgagc gcgagtcacc tcggcctag
Details for MyleA.01187.a.A1.GE34130
HARVESTED ON: 12/6/2011
SEQUENCED ON: 10/23/2013
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 58
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAVTSDG TPQAAKVRVR FCPSPTGVPH VGMVRTALFN WAYARHTGGT FVLRIEDTDA DRDSEESYLA LLDALRWLGL NWDEGPEVGG PYGPYRQSQR TDIYREVVAK LLATGEAYYA FSTPEEVENR HLAAGRNPKL GYDNFDRDLT DAQFSAYLAE GRKPVVRLRM PDEDISWDDL VRGTTTFAVG TVPDYVLTRA SGDPLYTLVN PCDDALMKIT HVLRGEDLLS STPRQVALYQ ALIRIGMAXR IPEFGHFPSV LGEGTKKLSK REPQSNLXAH RDRGFI
Validated NT Sequence
atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ctgcagtaac ctctgatggt accccgcaag cagcaaaagt cagggtacga ttctgcccat cgcccactgg tgttccgcac gtcgggatgg ttcgcacggc cctgttcaac tgggcttacg ctcgacatac tggaggcacc ttcgtcttac gcatcgagga caccgacgcc gaccgcgaca gtgaagaaag ctacctggcc ctgctcgatg cgttgcgctg gctcggcctg aactgggacg agggtcccga ggttggagga ccttacggtc cgtaccggca gtcgcagcgc actgacatct accgcgaggt ggtggcaaag ttgcttgcta caggagaggc ctattacgct ttctccacac cggaggaagt cgagaaccgc catcttgctg ctggacgcaa tcccaagctg ggttatgaca actttgaccg cgatcttacc gatgcgcagt tctcagcgta cttggcggaa ggccgtaagc ctgtagttcg gctgcggatg cccgacgagg atatcagttg ggatgacctg gtgcgcggga caactacctt tgctgtgggc actgtgcccg attacgtgtt gacccgagca agcggagatc cgttgtacac tttggtcaac ccgtgcgatg acgcgctgat gaagatcacg catgtactgc ggggcgaaga cttactatcg tcgactccgc gacaggtggc gctataccag gcgttgatcc gaatcgggat ggctgancgt attcccgaat tcggtcattt cccatcggta ttgggggagg gaaccaagaa actctcgaag cgagaaccgc agtcgaattt gnttgcccac cgtgaccggg gctttatccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVTAVTSDG TPQAAKVRVR FCPSPTGVPH VGMVRTALFN WAYARHTGGT FVLRIEDTDA DRDSEESYLA LLDALRWLGL NWDEGPEVGG PYGPYRQSQR TDIYREVVAK LLATGEAYYA FSTPEEVENR HLAAGRNPKL GYDNFDRDLT DAQFSAYLAE GRKPVVRLRM PDEDISWDDL VRGTTTFAVG TVPDYVLTRA SGDPLYTLVN PCDDALMKIT HVLRGEDLLS STPRQVALYQ ALIRIGMAER IPEFGHFPSV LGEGTKKLSK REPQSNLFAH RDRGFIPEGL LNYLALLGWA IADDHDLFSL DEMVAAFDVV DVNSNPARFD QKKADAVNAE HIRMLDSEDF AGRLRDYFTT HGYHIALDPA NYEAGFVAAA QLVQTRIVVL GDAWDLLKFL NDDEYSIDSK AAAKELDADA GPVLDVACAV LDSLVDWTTA SIEDVLKVAL IEGLGLKPRK VFGPIRVAAT GALVSPPLFE SLELLGRARS LQRLSAARAR VTSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgactgca gtaacctctg atggtacccc gcaagcagca aaagtcaggg tacgattctg cccatcgccc actggtgttc cgcacgtcgg gatggttcgc acggccctgt tcaactgggc ttacgctcga catactggag gcaccttcgt cttacgcatc gaggacaccg acgccgaccg cgacagtgaa gaaagctacc tggccctgct cgatgcgttg cgctggctcg gcctgaactg ggacgagggt cccgaggttg gaggacctta cggtccgtac cggcagtcgc agcgcactga catctaccgc gaggtggtgg caaagttgct tgctacagga gaggcctatt acgctttctc cacaccggag gaagtcgaga accgccatct tgctgctgga cgcaatccca agctgggtta tgacaacttt gaccgcgatc ttaccgatgc gcagttctca gcgtacttgg cggaaggccg taagcctgta gttcggctgc ggatgcccga cgaggatatc agttgggatg acctggtgcg cgggacaact acctttgctg tgggcactgt gcccgattac gtgttgaccc gagcaagcgg agatccgttg tacactttgg tcaacccgtg cgatgacgcg ctgatgaaga tcacgcatgt actgcggggc gaagacttac tatcgtcgac tccgcgacag gtggcgctat accaggcgtt gatccgaatc gggatggctg agcgtattcc cgaattcggt catttcccat cggtattggg ggagggaacc aagaaactct cgaagcgaga accgcagtcg aatttgtttg cccaccgtga ccggggcttt atccccgaag gtctgctgaa ctaccttgcc ttgctggggt gggcgatcgc cgatgaccac gacttgttca gtctcgatga gatggtagcc gcgttcgatg tggtcgacgt caactccaat ccggcgcgat tcgatcagaa gaaggccgat gcggttaacg ccgagcacat ccgaatgctc gactccgagg atttcgctgg caggctacgc gactacttca ccacacacgg ctaccacatc gctttggatc ccgctaatta cgaagctggt ttcgttgccg cggcgcagtt ggtgcagacc cgcatcgttg tgcttggtga cgcttgggac ctgttgaaat tcctcaacga tgacgagtac tctatcgact ctaaggccgc tgccaaagaa ctggatgccg atgccgggcc ggtgcttgat gttgcctgtg ccgtgctgga cagcctggtg gattggacca ctgcgagtat cgaggacgtc ctcaaggttg cactcatcga aggtcttggt cttaaaccgc gcaaagtgtt tggcccgatc cgggtcgccg ccacgggggc attggtcagc ccgccgctgt tcgaatcgct ggagctactc ggccgtgctc gcagtctgca gcggctgagt gctgcgcgag cgcgagtcac ctcggcctaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa