MyleA.01147.b

Cystathionine [beta]-synthase

CENTER ID: MyleA.01147.b
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.01147.b.A1.GE29254 Full length( MyleA.01147.b ) 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4630
RefSeq: YP_002504182.1
UniProt: A0A0H3N0Y2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIAQHISDL IGGTPLVRLN SVVPAGTGVV AAKVEYFNSG GSSKDRIAAK MIDAAEASGQ LKPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLLAYG AEVVVCPTAV PPDDPDSYYS VSDRLVAKID GAWKPDQYAN PAGPASHYET TGPEIWADTD GRVTHFVAGI GTGGTITGVG RYLKEVSGGL VRIIGADPEG SVYSGGTGRP YLVEGVGEDF WPAAYDPMVP DQIIAVSDSD SFNMTRRLAR EEAMLVGGSC GMAVVAALQV AEEAGPAALV VVLLPDGGRG YMSKIFNDAW MSSYGFLRSR LDGSTEQPIV GDVLRRKSGA LPDLVHTHPS ETVRDAIGIL REYAVSQIPV VGAEPPVMAG EVVGSVSERE LLSAVFEGRA SLADAVSEHM GPPLRLIGDG ELVSVAGEAL RDSDALMVVE KGKLVGVITR YDLLGFLSEG APLR
NT Sequence
ATGCGGATTG CGCAGCACAT CAGTGACCTC ATCGGCGGCA CGCCACTGGT CCGCCTGAAT TCTGTCGTCC CGGCCGGTAC CGGGGTCGTA GCGGCAAAGG TCGAATATTT CAACTCCGGG GGCAGCTCCA AGGACCGGAT CGCGGCTAAA ATGATCGATG CGGCAGAGGC CAGCGGTCAG CTGAAGCCGG GCGGCACCAT TGTCGAACCT ACGTCGGGTA ATACCGGTGT TGGTCTGGCT TTAGTCGCCC AGCATCGCGG GTATAAGTGT GTGTTCGTCT GCCCCGACAA AGTTAGCGAG GACAAACGGA ATGTGCTGTT GGCCTACGGA GCCGAGGTAG TCGTATGTCC GACGGCCGTG CCGCCCGACG ATCCGGACAG CTACTACAGC GTTTCCGACC GGTTGGTCGC CAAGATCGAC GGTGCCTGGA AACCAGACCA GTACGCCAAC CCAGCAGGAC CGGCCAGCCA TTACGAGACC ACTGGCCCGG AGATTTGGGC CGACACCGAC GGTAGGGTAA CCCATTTCGT CGCTGGCATC GGTACCGGTG GGACAATCAC CGGCGTGGGC CGCTACCTCA AGGAGGTGTC CGGCGGATTG GTACGTATCA TCGGTGCCGA TCCGGAAGGG TCAGTGTATT CGGGCGGTAC CGGACGGCCA TATCTGGTCG AGGGGGTGGG CGAGGACTTC TGGCCGGCGG CGTACGACCC GATGGTGCCC GATCAGATCA TCGCGGTGTC TGACTCCGAC TCGTTCAATA TGACCAGGCG GTTGGCCCGC GAAGAAGCGA TGCTGGTCGG CGGGTCGTGT GGCATGGCGG TCGTGGCTGC TCTGCAAGTG GCCGAAGAAG CCGGGCCGGC CGCGCTGGTC GTGGTCTTGC TGCCGGACGG CGGCCGTGGC TACATGTCCA AGATCTTCAA TGACGCCTGG ATGTCGTCGT ATGGGTTCCT GCGTAGCCGT CTGGACGGGT CTACCGAGCA GCCCATAGTG GGTGATGTGT TGCGTCGGAA ATCGGGCGCG TTGCCCGACT TGGTGCACAC CCATCCGTCG GAGACGGTGC GCGATGCCAT TGGCATTCTG CGTGAGTACG CGGTATCCCA GATCCCGGTT GTCGGCGCCG AGCCTCCGGT GATGGCGGGC GAGGTTGTCG GCAGCGTCTC CGAACGGGAA CTACTTTCGG CCGTGTTTGA AGGTCGTGCC AGCTTGGCCG ACGCCGTCTC GGAGCACATG GGCCCGCCAC TACGACTAAT CGGCGACGGT GAGTTGGTTA GTGTGGCCGG TGAGGCGCTG CGCGACTCCG ATGCGCTGAT GGTGGTGGAG AAAGGCAAAC TGGTCGGGGT CATAACGCGA TACGACTTGT TGGGTTTCCT CTCGGAGGGC GCACCGCTAC GT
Details for MyleA.01147.b.A1.GE29254
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIAQHISD LIGGTPLVRL NSVVPAGTGV VAAKVEYFNS GGSSKDRIAA KMIDAAEASG QLKPGGTIVE PTSGNTGVGL ALVAQHRGYK CVFVCPDKVS EDKRNVLLAY GAEVVVCPTA VPPDDPDSYY SVSDRLVAKI DGAWKPDQYA NPAGPASHYE TTGPEIWADT DGRVTHFVAG IGTGGTITGV GRYLKEVSGG LVRIIGADPE GSVYSGGTGR PYLVEGVGED FWPAAYDPMV PDQIIAVSDS DSFNMTRRLA REEAMLVGGS CGMAVVAALQ VAEEAGPAAL VVVLLPDGGR GYMSKIFNDA WMSSYGFLRS RLDGSTEQPI VGDVLRRKSG ALPDLVHTHP SETVRDAIGI LREYAVSQIP VVGAEPPVMA GEVVGSVSER ELLSAVFEGR ASLADAVSEH MGPPLRLIGD GELVSVAGEA LRDSDALMVV EKGKLVGVIT RYDLLGFLSE GAPLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggattgcg cagcacatca gtgacctcat cggcggcacg ccactggtcc gcctgaattc tgtcgtcccg gccggtaccg gggtcgtagc ggcaaaggtc gaatatttca actccggggg cagctccaag gaccggatcg cggctaaaat gatcgatgcg gcagaggcca gcggtcagct gaagccgggc ggcaccattg tcgaacctac gtcgggtaat accggtgttg gtctggcttt agtcgcccag catcgcgggt ataagtgtgt gttcgtctgc cccgacaaag ttagcgagga caaacggaat gtgctgttgg cctacggagc cgaggtagtc gtatgtccga cggccgtgcc gcccgacgat ccggacagct actacagcgt ttccgaccgg ttggtcgcca agatcgacgg tgcctggaaa ccagaccagt acgccaaccc agcaggaccg gccagccatt acgagaccac tggcccggag atttgggccg acaccgacgg tagggtaacc catttcgtcg ctggcatcgg taccggtggg acaatcaccg gcgtgggccg ctacctcaag gaggtgtccg gcggattggt acgtatcatc ggtgccgatc cggaagggtc agtgtattcg ggcggtaccg gacggccata tctggtcgag ggggtgggcg aggacttctg gccggcggcg tacgacccga tggtgcccga tcagatcatc gcggtgtctg actccgactc gttcaatatg accaggcggt tggcccgcga agaagcgatg ctggtcggcg ggtcgtgtgg catggcggtc gtggctgctc tgcaagtggc cgaagaagcc gggccggccg cgctggtcgt ggtcttgctg ccggacggcg gccgtggcta catgtccaag atcttcaatg acgcctggat gtcgtcgtat gggttcctgc gtagccgtct ggacgggtct accgagcagc ccatagtggg tgatgtgttg cgtcggaaat cgggcgcgtt gcccgacttg gtgcacaccc atccgtcgga gacggtgcgc gatgccattg gcattctgcg tgagtacgcg gtatcccaga tcccggttgt cggcgccgag cctccggtga tggcgggcga ggttgtcggc agcgtctccg aacgggaact actttcggcc gtgtttgaag gtcgtgccag cttggccgac gccgtctcgg agcacatggg cccgccacta cgactaatcg gcgacggtga gttggttagt gtggccggtg aggcgctgcg cgactccgat gcgctgatgg tggtggagaa aggcaaactg gtcggggtca taacgcgata cgacttgttg ggtttcctct cggagggcgc accgctacgt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa