SSGCID: Target Page

MyleA.01147.a

Cysteine synthase

CENTER ID:	MyleA.01147.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01147.a.A1.GE29253	Full length( MyleA.01147.a )		1	310

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1558
RefSeq:	YP_002503263.1
UniProt:	A0A0H3MQA6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIAEDVTQL VGNTPLVRLR RVTEGALADV VAKLESFNPA NSVKDRIGVA MIDTAEEAGL IKPDTVILEP TSGNTGIALA MVCAARGYHC VLTMPETMSI ERRMLLRAYG AELILTPGAD GMSGAIAKAE ELAKSDKRYF IPQQFENPAN PAIHAATTAE EVWRDTDGKV DIFVSGIGTG GTITGVARII KERRPATQFV AVEPAASPVL SGGQKGPHPI QGIGAGFVPS VLVLDLVDEI VTVGNEEALD LARRLAKEEG LLVGISAGAA VCAALHVARR PENTGKLIVV VLPDFGERYL STMLFADLAN

NT Sequence

ATGAGCATTG CTGAAGACGT CACACAACTA GTTGGTAACA CGCCACTGGT CCGGCTACGC CGAGTTACCG AAGGTGCGCT GGCTGACGTT GTCGCCAAGC TGGAATCCTT CAATCCCGCC AACAGCGTGA AGGACAGGAT AGGCGTGGCC ATGATCGACA CGGCCGAGGA AGCGGGTTTG ATCAAGCCGG ACACCGTCAT CCTTGAGCCG ACGAGCGGGA ACACCGGCAT CGCACTAGCT ATGGTATGCG CAGCACGTGG CTACCACTGC GTGCTGACCA TGCCCGAGAC AATGAGCATC GAACGACGAA TGTTGTTGCG AGCGTACGGC GCCGAACTCA TCTTGACTCC GGGCGCCGAT GGCATGTCAG GAGCCATCGC CAAGGCTGAG GAATTAGCCA AGTCCGATAA AAGGTATTTT ATACCACAGC AATTCGAGAA CCCAGCGAAC CCAGCCATCC ACGCCGCCAC AACCGCCGAG GAAGTGTGGC GCGACACCGA TGGCAAGGTA GACATCTTCG TTTCTGGAAT CGGGACCGGA GGCACTATCA CTGGTGTTGC CCGAATCATT AAAGAACGCC GGCCCGCCAC GCAGTTCGTC GCCGTAGAGC CCGCCGCGTC GCCGGTGTTA TCCGGTGGCC AGAAGGGTCC TCACCCCATT CAGGGCATCG GTGCTGGATT CGTCCCGTCG GTGCTGGTCC TGGACCTGGT GGACGAGATC GTTACCGTCG GCAACGAAGA AGCGCTCGAC CTAGCCCGCA GACTGGCTAA GGAAGAGGGA CTGCTGGTCG GCATCTCCGC AGGGGCGGCC GTTTGCGCCG CCCTGCACGT GGCCCGCCGG CCAGAGAATA CAGGAAAACT GATCGTCGTC GTGCTGCCCG ATTTCGGCGA GCGATATTTA AGCACGATGC TATTCGCGGA CTTAGCCAAC

Details for MyleA.01147.a.A1.GE29253

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	34kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

tagttggcta agtccgcgaa tagcatcgtg cttaaatatc gctcgccgaa atcgggcagc acgacgacga tcagttttcc tgtattctct ggccggcggg ccacgtgcag ggcggcgcaa acggccgccc ctgcggagat gccgaccagc agtccctctt ccttagccag tctgcgggct aggtcgagcg cttcttcgtt gccgacggta acgatctcgt ccaccaggtc caggaccagc accgacggga cgaatccagc accgatgccc tgaatggggt gaggaccctt ctggccaccg gataacaccg gcgacgcggc gggctctacg gcgacgaact gcgtggcggg ccggcgttct ttaatgattc gggcaacacc agtgatagtg cctccggtcc cgattccaga aacgaagatg tctaccttgc catcggtgtc gcgccacact tcctcggcgg ttgtggcggc gtggatggct gggttcgctg ggttctcgaa ttgctgtggt ataaaatacc ttttatcgga cttggctaat tcctcagcct tggcgatggc tcctgacatg ccatcggcgc ccggagtcaa gatgagttcg gcgccgtacg ctcgcaacaa cattcgtcgt tcgatgctca ttgtctcggg catggtcagc acgcagtggt agccacgtgc tgcgcatacc atagctagtg cgatgccggt gttcccgctc gtcggctcaa ggatgacggt gtccggcttg atcaaacccg cttcctcggc cgtgtcgatc atggccacgc ctatcctgtc cttcacgctg ttggcgggat tgaaggattc cagcttggcg acaacgtcag ccagcgcacc ttcggtaact cggcgtagcc ggaccagtgg cgtgttacca actagttgtg tgacgtcttc agcaatgctc atcgaaccag gaccctgggt ctgagcttcc agggtac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMSIAEDVTQ LVGNTPLVRL RRVTEGALAD VVAKLESFNP ANSVKDRIGV AMIDTAEEAG LIKPDTVILE PTSGNTGIAL AMVCAARGYH CVLTMPETMS IERRMLLRAY GAELILTPGA DGMSGAIAKA EELAKSDKRY FIPQQFENPA NPAIHAATTA EEVWRDTDGK VDIFVSGIGT GGTITGVARI IKERRPATQF VAVEPAASPV LSGGQKGPHP IQGIGAGFVP SVLVLDLVDE IVTVGNEEAL DLARRLAKEE GLLVGISAGA AVCAALHVAR RPENTGKLIV VVLPDFGERY LSTMLFADLA N

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcattgct gaagacgtca cacaactagt tggtaacacg ccactggtcc ggctacgccg agttaccgaa ggtgcgctgg ctgacgttgt cgccaagctg gaatccttca atcccgccaa cagcgtgaag gacaggatag gcgtggccat gatcgacacg gccgaggaag cgggtttgat caagccggac accgtcatcc ttgagccgac gagcgggaac accggcatcg cactagctat ggtatgcgca gcacgtggct accactgcgt gctgaccatg cccgagacaa tgagcatcga acgacgaatg ttgttgcgag cgtacggcgc cgaactcatc ttgactccgg gcgccgatgg catgtcagga gccatcgcca aggctgagga attagccaag tccgataaaa ggtattttat accacagcaa ttcgagaacc cagcgaaccc agccatccac gccgccacaa ccgccgagga agtgtggcgc gacaccgatg gcaaggtaga catcttcgtt tctggaatcg ggaccggagg cactatcact ggtgttgccc gaatcattaa agaacgccgg cccgccacgc agttcgtcgc cgtagagccc gccgcgtcgc cggtgttatc cggtggccag aagggtcctc accccattca gggcatcggt gctggattcg tcccgtcggt gctggtcctg gacctggtgg acgagatcgt taccgtcggc aacgaagaag cgctcgacct agcccgcaga ctggctaagg aagagggact gctggtcggc atctccgcag gggcggccgt ttgcgccgcc ctgcacgtgg cccgccggcc agagaataca ggaaaactga tcgtcgtcgt gctgcccgat ttcggcgagc gatatttaag cacgatgcta ttcgcggact tagccaacaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa