SSGCID: Target Page

MyleA.01136.a

PROBABLE 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE DXR (DXP REDUCTOISOMERASE) (1-DEOXYXYLULOSE-5-PHOSPHATE REDUCTOISOMERAS

CENTER ID:	MyleA.01136.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01136.a.A1.GE34129	full length		1	406

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2993
RefSeq:	YP_002503724.1
UniProt:	B8ZRU7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNPIEGHAG GRLRVLVLGS TGSIGTQALE VIAANPDRFE VVGLAAGGAQ LDTLLRQRAA TGVTNIAIAD DRAAQLAGDI PYHGTDAVTR LVEETEADVV LNALVGALGL RPTLAALHTG ARLALANKES LVAGGSLVLA AAQPGQIVPV DSEHSALAQC LRGGTPDEVA KLVLTASGGP FRGWNAGDLE RVTPEQAGVH PTWSMGTMNT LNSASLVNKG LELIEANLLF GIPYDRIEVV VHPQSIVHSM VTFIDGSTIA QASPPDMKLP ISLALGWPQR VGGAARACAF TTASTWEFEP LDIDVFPAVE LARHAGQIGG CMTAIYDAAN EEAAEAFLQG RIGFPAIVAT IADVLQRADQ WAPQWGEGPA TVDDVLDAQR WARERALCAV ATASSGKVSD MVLERS

NT Sequence

gtgaacaatc cgatcgaggg gcacgctggc ggccgcctcc gcgtgctggt gttgggaagt actggctcaa ttggcaccca ggcgctggaa gttatcgccg ccaatccgga ccgtttcgag gtagtcgggc tggccgccgg gggcgcgcag ctggacacgc tgctgaggca gcgcgccgcg accggcgtca ccaatatcgc catcgctgac gatcgcgcgg ctcagctggc cggcgacatc ccttaccacg ggaccgatgc ggtcacccgg ctggttgagg agactgaggc tgacgttgtc ctcaatgcgc tggtcggggc attgggtctg cgacccacac tggctgcact gcacacgggc gcgcgattgg cgttggccaa caaggaatcg ctggtagctg gcggttcgct ggtgttggcc gcggcgcagc caggccagat cgtgcccgta gactcggaac actccgcgct ggcgcaatgc ctgcgcggtg gtacccccga cgaagttgct aagttagtgc taaccgcctc cggcgggccg tttcgtggct ggaacgccgg cgacttggag cgcgttacac ccgagcaggc gggcgtccat ccgacttggt caatggggac gatgaacacg ctgaactcag cgtctctggt taacaagggg ctcgagctca tcgaagccaa cctgttgttc ggcattccct acgaccgcat tgaggtggtt gtgcaccctc agtcaattgt tcattcgatg gtgacattca tcgacggctc gacgatcgcc caagccagcc ctccggacat gaagctacct atttctttgg cgttgggctg gccacagcgg gtgggtggcg ctgctcgagc ctgtgctttc actaccgcat ctacctggga attcgagccg ctggacatcg atgtttttcc cgcagtcgag ctggcccggc acgctggaca gatcggcggc tgtatgaccg ccatttacga tgctgctaat gaggaggctg cagaggcctt cctccaaggt cggatcggct tccccgccat cgtcgcaaca atcgcggatg tgttgcagcg tgccgaccaa tgggctcccc aatggggtga gggacccgct actgtggatg atgtactcga cgcgcagcgc tgggcccgtg agcgagcgtt gtgtgcggta gcaacagcga gttctggaaa ggtctctgac atggtcttag aaaggtccta a

Details for MyleA.01136.a.A1.GE34129

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	44kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	87
PERCENT COVERAGE:	78

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVNNPIEGH AGGRLRVLVL GSTGSIGTQA LEVIAANPDR FEVVGLAAGG AQLDTLLRQR AATGVTNIAI ADDRAAQLAG DIPYHGTDAV TRLVEETEAD VVLNALVGAL GLRPTLAALH TGARLALANK ESLVAGGSLV LAAAQPGQIV PVDSEHSALA QCLRGGTPDE VAKLVLTASG GPFRGWNAGD LERVTPEQAG VHPTWSMGTM NTLNSASLVN KGLELIEANL LFGIPYDRIE VVVHPQSIVH SMVTFIDGST IAQASPPDXX LPXLXXWAGX SGGWXCSSXA FTTXLXVXXX XSXFXXXXXX XXLXXXXX

Validated NT Sequence

tttgtttnnc tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgaa caatccgatc gaggggcacg ctggcggccg cctccgcgtg ctggtgttgg gaagtactgg ctcaattggc acccaggcgc tggaagttat cgccgccaat ccggaccgtt tcgaggtagt cgggctggcc gccgggggcg cgcagctgga cacgctgctg aggcagcgcg ccgcgaccgg cgtcaccaat atcgccatcg ctgacgatcg cgcggctcag ctggccggcg acatccctta ccacgggacc gatgcggtca cccggctggt tgaggagact gaggctgacg ttgtcctcaa tgcgctggtc ggggcattgg gtctgcgacc cacactggct gcactgcaca cgggcgcgcg attggcgttg gccaacaagg aatcgctggt agctggcggt tcgctggtgt tggccgcggc gcagccaggc cagatcgtgc ccgtagactc ggaacactcc gcgctggcgc aatgcctgcg cggtggtacc cccgacgaag ttgctaagtt agtgctaacc gcctccggcg ggccgtttcg tggctggaac gccggcgact tggagcgcgt tacacccgag caggcgggcg tccatccgac ttggtcaatg gggacgatga acacgctgaa ctcagcgtct ctggttaaca aggggctcga gctcatcgaa gccaacctgt tgttcggcat tccctacgac cgcattgagg tggttgtgca ccctcagtca attgttcatt cgatggtgac attcatcgac ggntcgacna tcgcccaagc cagccctccg gacntgaanc tacctannct ttnncnttgg gctggcnaca gcggtgggtg gnnctgctcg agcngtgcnt tcactacnna tctncnngtc nancnnnnnn natcgangtt ttncnncant cnancnngnn nnnnnctgnn nnnnngnnnn gnntgacnnn cntttncnan gnnncnannn gnangntgnn nnnntnnnnc cnngnngnnn nnnnncnnnn nnnnncnnnn nannnnnann gntnnnnnnn nnnnngnnnn cnnnnnnnng nnnnnncnnn nannnnnnnn nnnnnnnnnn nna

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVNNPIEGH AGGRLRVLVL GSTGSIGTQA LEVIAANPDR FEVVGLAAGG AQLDTLLRQR AATGVTNIAI ADDRAAQLAG DIPYHGTDAV TRLVEETEAD VVLNALVGAL GLRPTLAALH TGARLALANK ESLVAGGSLV LAAAQPGQIV PVDSEHSALA QCLRGGTPDE VAKLVLTASG GPFRGWNAGD LERVTPEQAG VHPTWSMGTM NTLNSASLVN KGLELIEANL LFGIPYDRIE VVVHPQSIVH SMVTFIDGST IAQASPPDMK LPISLALGWP QRVGGAARAC AFTTASTWEF EPLDIDVFPA VELARHAGQI GGCMTAIYDA ANEEAAEAFL QGRIGFPAIV ATIADVLQRA DQWAPQWGEG PATVDDVLDA QRWARERALC AVATASSGKV SDMVLERS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacaat ccgatcgagg ggcacgctgg cggccgcctc cgcgtgctgg tgttgggaag tactggctca attggcaccc aggcgctgga agttatcgcc gccaatccgg accgtttcga ggtagtcggg ctggccgccg ggggcgcgca gctggacacg ctgctgaggc agcgcgccgc gaccggcgtc accaatatcg ccatcgctga cgatcgcgcg gctcagctgg ccggcgacat cccttaccac gggaccgatg cggtcacccg gctggttgag gagactgagg ctgacgttgt cctcaatgcg ctggtcgggg cattgggtct gcgacccaca ctggctgcac tgcacacggg cgcgcgattg gcgttggcca acaaggaatc gctggtagct ggcggttcgc tggtgttggc cgcggcgcag ccaggccaga tcgtgcccgt agactcggaa cactccgcgc tggcgcaatg cctgcgcggt ggtacccccg acgaagttgc taagttagtg ctaaccgcct ccggcgggcc gtttcgtggc tggaacgccg gcgacttgga gcgcgttaca cccgagcagg cgggcgtcca tccgacttgg tcaatgggga cgatgaacac gctgaactca gcgtctctgg ttaacaaggg gctcgagctc atcgaagcca acctgttgtt cggcattccc tacgaccgca ttgaggtggt tgtgcaccct cagtcaattg ttcattcgat ggtgacattc atcgacggct cgacgatcgc ccaagccagc cctccggaca tgaagctacc tatttctttg gcgttgggct ggccacagcg ggtgggtggc gctgctcgag cctgtgcttt cactaccgca tctacctggg aattcgagcc gctggacatc gatgtttttc ccgcagtcga gctggcccgg cacgctggac agatcggcgg ctgtatgacc gccatttacg atgctgctaa tgaggaggct gcagaggcct tcctccaagg tcggatcggc ttccccgcca tcgtcgcaac aatcgcggat gtgttgcagc gtgccgacca atgggctccc caatggggtg agggacccgc tactgtggat gatgtactcg acgcgcagcg ctgggcccgt gagcgagcgt tgtgtgcggt agcaacagcg agttctggaa aggtctctga catggtctta gaaaggtcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa