SSGCID: Target Page

MyleA.01124.a

Probable antibiotic resistance efflux protein

CENTER ID:	MyleA.01124.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01124.a.A1.GE29251	Full length( MyleA.01124.a )		1	331

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.4529
RefSeq:	YP_002504161.1
UniProt:	A0A0H3N0W7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRNNDKAVMV RGVRKTYGKG KIIALDDVSF DVDRGEVIGL LGPNGAGKTT MVDILSTLTR PDRGLATVAG YDVVSEPAGV RRSIMVTGQQ VAVDDQLSGE QNLILFGRLY GLSKSVARVR TQELLEQFDL LHAGKRRVGT YSGGMRRRID IACGLVVQPQ VAFLDEPTTG LDPRSRQAIW DLVASFKQIG IATLLTTQYL EEADELSDRI ILIDHGTVIA EGTASELKHR VGETFCEIVP RDLKDLGTVV AALGSLLPER SRAMLTPTSD RITMPAPDGT QTLIEAARRI DEVSVELADI ALRRPSLDDV FLSMTTDPSE SLSHLAAGAM R

NT Sequence

ATGCGCAACA ACGACAAGGC CGTGATGGTT CGCGGGGTTC GTAAGACCTA CGGCAAGGGC AAGATTATCG CCCTGGATGA TGTTAGTTTC GACGTGGATC GCGGTGAAGT GATCGGTCTG CTCGGCCCGA ACGGTGCCGG CAAGACGACC ATGGTGGACA TCTTGTCGAC GTTGACCCGG CCGGATCGTG GCTTGGCTAC CGTCGCCGGC TACGACGTGG TTTCCGAACC GGCCGGGGTG CGTCGCTCGA TCATGGTCAC CGGACAGCAA GTAGCTGTCG ATGACCAGCT TTCCGGTGAG CAGAACCTAA TTTTATTCGG TCGTCTGTAT GGGCTGAGCA AATCTGTGGC GCGCGTGCGG ACGCAAGAAC TGCTCGAGCA ATTTGACCTC CTGCACGCCG GGAAGCGGCG GGTGGGCACG TACTCGGGCG GGATGCGTCG GCGGATAGAT ATCGCGTGCG GATTGGTGGT TCAACCCCAA GTGGCATTCT TGGATGAGCC CACAACCGGG TTGGATCCGC GGAGTCGGCA AGCTATTTGG GATCTAGTGG CCAGTTTCAA ACAGATCGGT ATTGCCACAC TGCTGACTAC GCAGTATCTC GAGGAGGCGG ATGAGCTCAG TGATCGGATC ATCTTGATCG ACCACGGTAC GGTCATTGCA GAAGGCACCG CGAGTGAGCT CAAGCACCGT GTTGGTGAGA CTTTTTGTGA AATAGTACCG CGCGATTTGA AGGATCTGGG CACTGTCGTC GCAGCGCTGG GTTCGCTGTT GCCCGAGCGC AGCAGGGCGA TGCTCACGCC CACGTCAGAC CGGATTACGA TGCCGGCGCC GGATGGCACG CAGACGCTTA TCGAGGCTGC ACGTCGGATC GACGAGGTGA GTGTTGAGCT GGCTGACATT GCGCTACGCC GGCCGTCGCT CGATGACGTA TTCCTTTCGA TGACAACGGA TCCCAGTGAG TCGCTGAGCC ATCTAGCGGC GGGGGCGATG CGA

Details for MyleA.01124.a.A1.GE29251

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	38kDa
OBSERVED MW:	38kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

accctggaag ctcagaccca gggtcctggt tcgatgcgca acaacgacaa ggccgtgatg gttcgcgggg ttcgtaagac ctacggcaag ggcaagatta tcgccctgga tgatgttagt ttcgacgtgg atcgcggtga agtgatcggt ctgctcggcc cgaacggtgc cggcaagacg accatggtgg acatcttgtc gacgttgacc cggccggatc gtggcttggc taccgtcgcc ggctacgacg tggtttccga accggccggg gtgcgtcgct cgatcatggt caccggacag caagtagctg tcgatgacca gctttccggt gagcagaacc taattttatt cggtcgtctg tatgggctga gcaaatctgt ggcgcgcgtg cggacgcaag aactgctcga gcaatttgac ctcctgcacg ccgggaagcg gcgggtgggc acgtactcgg gcgggatgcg tcggcggata gatatcgcgt gcggattggt ggttcaaccc caagtggcat tcttggatga gcccacaacc gggttggatc cgcggagtcg gcaagctatt tgggatctag tggccagttt caaacagatc ggtattgcca cactgctgac tacgcagtat ctcgaggagg cggatgagct cagtgatcgg atcatcttga tcgaccacgg tacggtcatt gcagaaggca ccgcgagtga gctcaagcac cgtgttggtg agactttttg tgaaatagta ccgcgcgatt tgaaggatct gggcactgtc gtcgcagcgc tgggttcgct gttgcccgag cgcagcaggg cgatgctcac gcccacgtca gaccggatta cgatgccggc gccggatggc acgcagacgc ttatcgaggc tgcacgtcgg atcgacgagg tgagtgttga gctggctgac attgcgctac gccggccgtc gctcgatgac gtattccttt cgatgacaac ggatcccagt gagtcgctga gccatctagc ggcgggggcg atgcgataat aa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRNNDKAVM VRGVRKTYGK GKIIALDDVS FDVDRGEVIG LLGPNGAGKT TMVDILSTLT RPDRGLATVA GYDVVSEPAG VRRSIMVTGQ QVAVDDQLSG EQNLILFGRL YGLSKSVARV RTQELLEQFD LLHAGKRRVG TYSGGMRRRI DIACGLVVQP QVAFLDEPTT GLDPRSRQAI WDLVASFKQI GIATLLTTQY LEEADELSDR IILIDHGTVI AEGTASELKH RVGETFCEIV PRDLKDLGTV VAALGSLLPE RSRAMLTPTS DRITMPAPDG TQTLIEAARR IDEVSVELAD IALRRPSLDD VFLSMTTDPS ESLSHLAAGA MR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcaacaac gacaaggccg tgatggttcg cggggttcgt aagacctacg gcaagggcaa gattatcgcc ctggatgatg ttagtttcga cgtggatcgc ggtgaagtga tcggtctgct cggcccgaac ggtgccggca agacgaccat ggtggacatc ttgtcgacgt tgacccggcc ggatcgtggc ttggctaccg tcgccggcta cgacgtggtt tccgaaccgg ccggggtgcg tcgctcgatc atggtcaccg gacagcaagt agctgtcgat gaccagcttt ccggtgagca gaacctaatt ttattcggtc gtctgtatgg gctgagcaaa tctgtggcgc gcgtgcggac gcaagaactg ctcgagcaat ttgacctcct gcacgccggg aagcggcggg tgggcacgta ctcgggcggg atgcgtcggc ggatagatat cgcgtgcgga ttggtggttc aaccccaagt ggcattcttg gatgagccca caaccgggtt ggatccgcgg agtcggcaag ctatttggga tctagtggcc agtttcaaac agatcggtat tgccacactg ctgactacgc agtatctcga ggaggcggat gagctcagtg atcggatcat cttgatcgac cacggtacgg tcattgcaga aggcaccgcg agtgagctca agcaccgtgt tggtgagact ttttgtgaaa tagtaccgcg cgatttgaag gatctgggca ctgtcgtcgc agcgctgggt tcgctgttgc ccgagcgcag cagggcgatg ctcacgccca cgtcagaccg gattacgatg ccggcgccgg atggcacgca gacgcttatc gaggctgcac gtcggatcga cgaggtgagt gttgagctgg ctgacattgc gctacgccgg ccgtcgctcg atgacgtatt cctttcgatg acaacggatc ccagtgagtc gctgagccat ctagcggcgg gggcgatgcg aaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa