SSGCID: Target Page

MyleA.01083.a

Thiosulfate sulfurtransferase

CENTER ID:	MyleA.01083.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01083.a.A1.GE29248	Full length( MyleA.01083.a )		1	277

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.4182
RefSeq:	YP_002504070.1
UniProt:	A0A0H3MRX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MARSDVLVSA DWAESNLDSA NIVFVEVDED TSTYDGDHIA GAIKLDWRAD LQDPIKRDFI DTQQFSKLLG DRGISNDNTV ILYGGNNNWF AAYAYWYFKL YRHDKVKLLD GGRKKWELDG RPLSTDTVTR PATSYAAAAP DNTIRAFRDE VIASIKIKNL VDVRSPDEFS GKLLAPAHLP QEQSQRPGHI PSAINIPWSK AANEDGTFKS DEQLAKLYAD AGLDRLKETI VYCRIGERSS HTWFVLRELL GYQNVKNYDG SWTEYGSLVG VPIELGS

NT Sequence

ATGGCACGCT CTGATGTCCT GGTCTCTGCC GACTGGGCCG AGAGCAACCT CGACAGCGCG AACATCGTCT TCGTCGAAGT GGACGAGGAC ACAAGCACCT ACGACGGCGA TCACATCGCC GGCGCCATCA AGTTGGATTG GCGTGCGGAT TTGCAAGACC CGATCAAGCG CGACTTCATC GACACCCAGC AATTCTCCAA ATTGTTGGGT GACCGAGGCA TTTCCAATGA CAACACCGTG ATTCTGTACG GCGGCAACAA CAACTGGTTC GCAGCCTATG CGTATTGGTA TTTCAAGCTG TACAGGCATG ACAAAGTCAA ATTGCTTGAC GGTGGCCGCA AGAAGTGGGA GCTCGACGGT CGCCCGCTGT CCACCGACAC CGTCACCAGG CCGGCGACCT CCTACGCTGC TGCAGCACCG GACAACACTA TCCGGGCCTT TCGGGACGAG GTCATAGCAT CCATCAAGAT CAAAAATCTC GTCGACGTAC GTTCCCCCGA CGAATTCTCC GGAAAGCTCC TGGCACCTGC GCACCTACCT CAGGAGCAGA GCCAGCGACC CGGCCACATC CCCAGTGCGA TAAACATACC TTGGAGCAAG GCGGCCAATG AGGACGGCAC CTTCAAATCC GACGAGCAGT TAGCCAAGCT GTATGCCGAC GCCGGCCTGG ATAGGTTGAA AGAGACAATC GTCTACTGCC GAATCGGTGA ACGATCATCA CACACCTGGT TCGTGCTGCG CGAGTTACTC GGATATCAAA ACGTCAAGAA CTACGACGGC AGTTGGACAG AATACGGCTC CCTGGTGGGT GTCCCAATCG AATTGGGAAG C

Details for MyleA.01083.a.A1.GE29248

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	33kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMARSDVLVS ADWAESNLDS ANIVFVEVDE DTSTYDGDHI AGAIKLDWRA DLQDPIKRDF IDTQQFSKLL GDRGISNDNT VILYGGNNNW FAAYAYWYFK LYRHDKVKLL DGGRKKWELD GRPLSTDTVT RPATSYAAAA PDNTIRAFRD EVIASIKIKN LVDVRSPDEF SGKLLAPAHL PQEQSQRPGH IPSAINIPWS KAANEDGTFK SDEQLAKLYA DAGLDRLKET IVYCRIGERS SHTWFVLREL LGYQNVKNYD GSWTEYGSLV GVPIELGS

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcac gctctgatgt cctggtctct gccgactggg ccgagagcaa cctcgacagc gcgaacatcg tcttcgtcga agtggacgag gacacaagca cctacgacgg cgatcacatc gccggcgcca tcaagttgga ttggcgtgcg gatttgcaag acccgatcaa gcgcgacttc atcgacaccc agcaattctc caaattgttg ggtgaccgag gcatttccaa tgacaacacc gtgattctgt acggcggcaa caacaactgg ttcgcagcct atgcgtattg gtatttcaag ctgtacaggc atgacaaagt caaattgctt gacggtggcc gcaagaagtg ggagctcgac ggtcgcccgc tgtccaccga caccgtcacc aggccggcga cctcctacgc tgctgcagca ccggacaaca ctatccgggc ctttcgggac gaggtcatag catccatcaa gatcaaaaat ctcgtcgacg tacgttcccc cgacgaattc tccggaaagc tcctggcacc tgcgcaccta cctcaggagc agagccagcg acccggccac atccccagtg cgataaacat accttggagc aaggcggcca atgaggacgg caccttcaaa tccgacgagc agttagccaa gctgtatgcc gacgccggcc tggataggtt gaaagagaca atcgtctact gccgaatcgg tgaacgatca tcacacacct ggttcgtgct gcgcgagtta ctcggatatc aaaacgtcaa gaactacgac ggcagttgga cagaatacgg ctccctggtg ggtgtcccaa tcgaattggg aagctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgctct gatgtcctgg tctctgccga ctgggccgag agcaacctcg acagcgcgaa catcgtcttc gtcgaagtgg acgaggacac aagcacctac gacggcgatc acatcgccgg cgccatcaag ttggattggc gtgcggattt gcaagacccg atcaagcgcg acttcatcga cacccagcaa ttctccaaat tgttgggtga ccgaggcatt tccaatgaca acaccgtgat tctgtacggc ggcaacaaca actggttcgc agcctatgcg tattggtatt tcaagctgta caggcatgac aaagtcaaat tgcttgacgg tggccgcaag aagtgggagc tcgacggtcg cccgctgtcc accgacaccg tcaccaggcc ggcgacctcc tacgctgctg cagcaccgga caacactatc cgggcctttc gggacgaggt catagcatcc atcaagatca aaaatctcgt cgacgtacgt tcccccgacg aattctccgg aaagctcctg gcacctgcgc acctacctca ggagcagagc cagcgacccg gccacatccc cagtgcgata aacatacctt ggagcaaggc ggccaatgag gacggcacct tcaaatccga cgagcagtta gccaagctgt atgccgacgc cggcctggat aggttgaaag agacaatcgt ctactgccga atcggtgaac gatcatcaca cacctggttc gtgctgcgcg agttactcgg atatcaaaac gtcaagaact acgacggcag ttggacagaa tacggctccc tggtgggtgt cccaatcgaa ttgggaagca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a