SSGCID: Target Page

MyleA.01032.a

Probable Tyrosyl-tRNA synthase tyrS (TYRRS)

CENTER ID:	MyleA.01032.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01032.a.A1.GE34117	full length		1	426

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.2506
RefSeq:	YP_002503596.1
UniProt:	A0A0H3MQT0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSILNELG WRGLVAQSTD LDALADELRR GPMTVYAGFD PTAPSLHVGH LVPLLVLRRF QRAGHRPIVL AGGATGMIGD PRDIGERTLN EAYTVAEWAE RIRGQLERFV EFDDVAKSRN GAIVVNNLEW TSPMSAIEFL RDVGKHFSIN LMLDRDTIRR RLNGQGISYT EFSYMLLQAN DYAELHQRHG CALQIGGSDQ WGNIIAGVRL VRQKLGATVH ALTVPLVTAA DGAKLGKSTG GGSLWLDPEM TSPYAWYQYF INTSDADVIR YLRWFTFLLP EELVELEQTT VSRPQERAAQ RRLATELTVL VHGAAATQAV EHASRALFGQ GELARLDEAT LATALRETVV AELKPGNPDG IVDLLVASGL SPSRGAARRT IDEGGVLVNN IRIQSEEWTP RTSDFLHGRW LVLRRGKRNI AGIERV

NT Sequence

gtgtcttcca gcatccttaa cgagctgggc tggcgtgggt tggtcgcgca gtccactgac ctcgatgctc tagccgacga gttgcggcgt ggaccgatga cagtctacgc cggtttcgac cccaccgcgc cgagcctgca tgtcggacac ttggtgccgc tgctggtatt gcggcgcttt caacgagccg gccaccggcc aatcgtgcta gcgggcgggg caaccggcat gattggtgat ccgcgcgaca tcggtgagcg caccctcaac gaggcgtaca ccgtcgccga atgggccgag cgaatccgcg gacagctgga gcgtttcgtc gaattcgacg atgtcgcgaa gtctaggaac ggcgccatcg ttgtcaacaa cctggaatgg acgagcccga tgtcggcgat cgagttcttg cgcgatgtcg gcaaacactt ttcgatcaac ttgatgctag atcgggacac aatccggcga cggttgaatg gccaagggat ttcttacacg gagttcagct acatgctgtt gcaagccaac gattatgctg aattgcatca gcgccatggc tgtgcgctgc agatcggcgg ttcggatcag tggggcaaca tcatcgccgg ggttcgcctg gtgcgacaaa agctcggcgc cacagtgcat gcgctcacag tcccgctggt gaccgctgcc gatggcgcca agctcggcaa atccaccgga ggtggcagtt tatggttgga cccagagatg accagcccgt acgcctggta ccaatacttc atcaacacct ccgacgctga tgtcattcgg tacctgcgtt ggttcacttt tttgttgccc gaagaactgg tcgagctgga gcagacgacg gtctcacgcc cacaggaacg tgctgcacaa cgtcgactcg ccaccgagct cacggttttg gtgcatggcg cagcggcaac ccaggctgtt gagcatgcca gcagggcatt gttcggtcag ggcgaacttg ctcgccttga cgaagcgacg ttggcgacgg cattgcgcga gacagtggtc gctgagctga aacccggcaa tcccgatggt attgtcgatc tgttggtggc cagcggtctg tcccctagca ggggtgcggc gcggcgcact atcgatgaag gcggggtgtt ggtcaacaac attcgcatcc agagcgagga atggacgccc cggacatcgg atttcctgca cggccgctgg ctggtgctgc gccgtggcaa gcggaacatc gccgggatag aaagagtata g

Details for MyleA.01032.a.A1.GE34117

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	49kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	91
PERCENT COVERAGE:	64

Validated AA Sequence

Validated NT Sequence

ttntnnnntt tangaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgtctt ccagcatcct taacgagctg ggctggcgtg ggttggtcgc gcagtccact gacctcgatg ctctagccga cgagttgcgg cgtggaccga tgacagtcta cgccggtttc gaccccaccg cgccgagcct gcatgtcgga cacttggtgc cgctgctggt attgcggcgc tttcaacgag ccggccaccg gccaatcgtg ctagcgggcg gggcaaccgg catgattggt gatccgcgcg acatcggtga gcgcaccctc aacgaggcgt acaccgtcgc cgaatgggcc gagcgaatcc gcggacagct ggagcgtttc gtcgaattcg acgatgtcgc gaagtctagg aacggcgcca tcgttgtcaa caacctggaa tggacgagcc cgatgtcggc gatcgagttc ttgcgcgatg tcggcaaaca cttttcgatc aacttgatgc tagatcggga cacaatccgg cgacggttga atggccaagg gatttcttac acggagttca gctacatgct gttgcaagcc aacgattatg ctgaattgca tcagcgccat ggctgtgcgc tgcagatcgg cggttcggat cagtggggca acatcatcgc cggggttcgc ctggtgcgac aaaagctcgg cgccacagtg catgcgctca cagtcccgct ggtgaccgct gccgatgggc gccaagctcg gcaaatccac cggaggtggc agtttatggt tggacccaga gatgaccagc ccgtacgcct ggtaccaata cttcatcaac acctccgacg ctgangtcat tcggtacctg cgttggttca cttttttggc ccgaanaact ggtnnagntn gagcananga cggnctnnnn ccnncagaac ntgctnnnnn ngtcnantcg ccnncganct cnnnntttnn nnnnangnnn nncnnnnncc nnntnntnag cangcnnnnn ngnntnnntn gnnnnggnnn nanttnnnnn nnnnnnnnaa annnnnnnnn nnngnnnnng nnnnnnnnnn nannnnnnan nnnnnnnnnn nnnnnnnngn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggnnnnnnnn nt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVSSSILNE LGWRGLVAQS TDLDALADEL RRGPMTVYAG FDPTAPSLHV GHLVPLLVLR RFQRAGHRPI VLAGGATGMI GDPRDIGERT LNEAYTVAEW AERIRGQLER FVEFDDVAKS RNGAIVVNNL EWTSPMSAIE FLRDVGKHFS INLMLDRDTI RRRLNGQGIS YTEFSYMLLQ ANDYAELHQR HGCALQIGGS DQWGNIIAGV RLVRQKLGAT VHALTVPLVT AADGAKLGKS TGGGSLWLDP EMTSPYAWYQ YFINTSDADV IRYLRWFTFL LPEELVELEQ TTVSRPQERA AQRRLATELT VLVHGAAATQ AVEHASRALF GQGELARLDE ATLATALRET VVAELKPGNP DGIVDLLVAS GLSPSRGAAR RTIDEGGVLV NNIRIQSEEW TPRTSDFLHG RWLVLRRGKR NIAGIERV

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtcttcc agcatcctta acgagctggg ctggcgtggg ttggtcgcgc agtccactga cctcgatgct ctagccgacg agttgcggcg tggaccgatg acagtctacg ccggtttcga ccccaccgcg ccgagcctgc atgtcggaca cttggtgccg ctgctggtat tgcggcgctt tcaacgagcc ggccaccggc caatcgtgct agcgggcggg gcaaccggca tgattggtga tccgcgcgac atcggtgagc gcaccctcaa cgaggcgtac accgtcgccg aatgggccga gcgaatccgc ggacagctgg agcgtttcgt cgaattcgac gatgtcgcga agtctaggaa cggcgccatc gttgtcaaca acctggaatg gacgagcccg atgtcggcga tcgagttctt gcgcgatgtc ggcaaacact tttcgatcaa cttgatgcta gatcgggaca caatccggcg acggttgaat ggccaaggga tttcttacac ggagttcagc tacatgctgt tgcaagccaa cgattatgct gaattgcatc agcgccatgg ctgtgcgctg cagatcggcg gttcggatca gtggggcaac atcatcgccg gggttcgcct ggtgcgacaa aagctcggcg ccacagtgca tgcgctcaca gtcccgctgg tgaccgctgc cgatggcgcc aagctcggca aatccaccgg aggtggcagt ttatggttgg acccagagat gaccagcccg tacgcctggt accaatactt catcaacacc tccgacgctg atgtcattcg gtacctgcgt tggttcactt ttttgttgcc cgaagaactg gtcgagctgg agcagacgac ggtctcacgc ccacaggaac gtgctgcaca acgtcgactc gccaccgagc tcacggtttt ggtgcatggc gcagcggcaa cccaggctgt tgagcatgcc agcagggcat tgttcggtca gggcgaactt gctcgccttg acgaagcgac gttggcgacg gcattgcgcg agacagtggt cgctgagctg aaacccggca atcccgatgg tattgtcgat ctgttggtgg ccagcggtct gtcccctagc aggggtgcgg cgcggcgcac tatcgatgaa ggcggggtgt tggtcaacaa cattcgcatc cagagcgagg aatggacgcc ccggacatcg gatttcctgc acggccgctg gctggtgctg cgccgtggca agcggaacat cgccgggata gaaagagtat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa