SSGCID: Target Page

MyleA.01019.b

DIHYDROPTEROATE SYNTHASE 1 FOLP (DHPS 1) (DIHYDROPTEROATE PYROPHOSPHORYLASE 1) (DIHYDROPTEROATE DIPHOSPHORYLASE 1)

CENTER ID:	MyleA.01019.b
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.01019.b.A1.GE34118	full length		1	291

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1914
RefSeq:	YP_002503411.1
UniProt:	A0A0H3MUW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQSMLCGRPV AADRQLIMAI VNRTPDSFYD RGATFSDEAA RAAAHRAVAE GADVIDVGGV KAGPGQGVDV DTEIARLVPF IEWLRSAYTD LLISVDTWRA EVARLACTAG ADLINDSWGG ADPAMHEVAA ELGAGLVCSH TGGALPRTRP FRVSYGTTTR GVVDDVIRQV TAAAERAVAA GVTRDSVLVD PTHDFGKNTF HGLLLLRHVD ELVKTGWPVL MSLSNKDFVG ETLGVGLTER LEGTLAATAL AAAAGVRMFR VHEVVATRRV LEMVASIQGT RPPTRTVRGL A

NT Sequence

gtgcagtcaa tgctgtgcgg ccggccggtc gcagcggatc gccaactgat catggcgatc gtcaaccgta ccccggattc gttctatgac cggggcgcga ccttcagtga cgaggctgct cgtgctgcag cgcaccgggc cgttgcggag ggcgccgatg tcatcgatgt cggcggcgtt aaagcagggc ctggtcaggg cgttgacgta gacaccgaga tcgcccggtt ggttccgttc atcgaatggc tacgcagcgc ctatacagac ctgctgatca gcgtagacac ctggcgagca gaggtagcaa gactggcttg cactgcgggc gcagacctga tcaacgacag ctggggtgga gccgacccgg ccatgcatga ggtcgccgct gagcttggcg cgggcctggt gtgttcgcac accggtggcg cgctgccccg cacgcgtccc tttcgggtga gttacggtac gacgacccgc ggtgtggtgg acgatgtgat tcgccaggtc actgcagctg ccgagcgcgc ggtcgcggcc ggggtaaccc gcgattcggt gttggtcgac ccgacccacg atttcggcaa gaacactttc cacgggctgc tgctgttgcg tcatgtagac gaacttgtta agaccgggtg gcccgtgctc atgtcgttga gcaataagga cttcgtcggg gagactctgg gcgtgggttt gacggaacgg ctagagggta cgttggcggc cactgcgttg gcggcggcag ctggcgtccg catgtttcgg gtgcacgagg tcgtagcgac caggcgggtt ctggagatgg tggcttcgat ccaggggacg cgtcccccaa cgcgcacggt gaggggactc gcatga

Details for MyleA.01019.b.A1.GE34118

HARVESTED ON:	12/6/2011
SEQUENCED ON:	10/23/2013
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	95

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVQSMLCGR PVAADRQLIM AIVNRTPDSF YDRGATFSDE AARAAAHRAV AEGADVIDVG GVKAGPGQGV DVDTEIARLV PFIEWLRSAY TDLLISVDTW RAEVARLACT AGADLINDSW GGADPAMHEV AAELGAGLVC SHTGGALPRT RPFRVSYGTT TRGVVDDVIR QVTAAAERAV AAGVTRDSVL VDPTHDFGKN TFHGLLLLRH VDELVKTGWP VLMSLSNKDF VGETLGVGLT ERLEGTLAAT ALAAAAGVRM FRVHXVVXTR RVLXMVASI

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtgc agtcaatgct gtgcggccgg ccggtcgcag cggatcgcca actgatcatg gcgatcgtca accgtacccc ggattcgttc tatgaccggg gcgcgacctt cagtgacgag gctgctcgtg ctgcagcgca ccgggccgtt gcggagggcg ccgatgtcat cgatgtcggc ggcgttaaag cagggcctgg tcagggcgtt gacgtagaca ccgagatcgc ccggttggtt ccgttcatcg aatggctacg cagcgcctat acagacctgc tgatcagcgt agacacctgg cgagcagagg tagcaagact ggcttgcact gcgggcgcag acctgatcaa cgacagctgg ggtggagccg acccggccat gcatgaggtc gccgctgagc ttggcgcggg cctggtgtgt tcgcacaccg gtggcgcgct gccccgcacg cgtccctttc gggtgagtta cggtacgacg acccgcggtg tggtggacga tgtgattcgc caggtcactg cagctgccga gcgcgcggtc gcggccgggg taacccgcga ttcggtgttg gtcgacccga cccacgattt cggcaagaac actttccacg ggctgctgct gttgcgtcat gtagacgaac ttgttaagac cgggtggccc gtgctcatgt cgttgagcaa taaggacttc gtcggggaga ctctgggcgt gggtttgacg gaacggctag agggtacgtt ggcggccact gcgttggcgg cggcagctgg cgtccgcatg tttcgggtgc acgangtcgt ancgaccagg cgggttctgg anatggtggc ttcgatcc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcagtca atgctgtgcg gccggccggt cgcagcggat cgccaactga tcatggcgat cgtcaaccgt accccggatt cgttctatga ccggggcgcg accttcagtg acgaggctgc tcgtgctgca gcgcaccggg ccgttgcgga gggcgccgat gtcatcgatg tcggcggcgt taaagcaggg cctggtcagg gcgttgacgt agacaccgag atcgcccggt tggttccgtt catcgaatgg ctacgcagcg cctatacaga cctgctgatc agcgtagaca cctggcgagc agaggtagca agactggctt gcactgcggg cgcagacctg atcaacgaca gctggggtgg agccgacccg gccatgcatg aggtcgccgc tgagcttggc gcgggcctgg tgtgttcgca caccggtggc gcgctgcccc gcacgcgtcc ctttcgggtg agttacggta cgacgacccg cggtgtggtg gacgatgtga ttcgccaggt cactgcagct gccgagcgcg cggtcgcggc cggggtaacc cgcgattcgg tgttggtcga cccgacccac gatttcggca agaacacttt ccacgggctg ctgctgttgc gtcatgtaga cgaacttgtt aagaccgggt ggcccgtgct catgtcgttg agcaataagg acttcgtcgg ggagactctg ggcgtgggtt tgacggaacg gctagagggt acgttggcgg ccactgcgtt ggcggcggca gctggcgtcc gcatgtttcg ggtgcacgag gtcgtagcga ccaggcgggt tctggagatg gtggcttcga tccaggggac gcgtccccca acgcgcacgg tgaggggact cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa