MyleA.00951.a

CONSERVED HYPOTHETICAL PROTEIN

CENTER ID: MyleA.00951.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00951.a.A1.GE34121 full length 1 468
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.3018
RefSeq: YP_002503729.1
UniProt: A0A0H3MR14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRVHAFGDD ALGDLDAVGL ADAIRAGWVS RADVIEAAIV RTEAVNPALG GLAYEAFQWA RQTASKAGSG FFSGVPTFIK DNIDVAGQPT MRGSDAWVPR NAFDDGEFTR LYLATGPVSL GKTQLSEFGF SASAEHMRLG PVRNPWDTDY TAGASSSGSG AFVAAGVVPM AHANDGGGSI RIPASCNGLV GLKPSRGRLP LDSELRRLPV GIVVNGVLTR SVRDTAAFYR EAERIWHNPK LPPVGDVTQP GRQRLRIAVV TRSVQRECSP ELRELTLKSA RLLEELGHRV ERVAEPPVPP NFPDDFLLYW GLLAAMQVRT GRLAFGNTFD RTKLDSLTLG LDRHASRNMH RLPKAIMRLR RLRRRTADFF ATYDVLLTPT VADETPRIGY LTPTDYQQVM DRLMGWVAFT PLQNVTGEPA ISLPLAQSAD GMPVGMMFTA DFGQEAQLLE LAFELEEARP WARIQIGD
NT Sequence
atgcaacgtg tgcacgcttt cggtgacgac gccctcggcg atctcgacgc ggtcggtcta gccgacgcta tccgagccgg ttgggtcagc agagccgatg tcatcgaggc ggccattgtg cggaccgagg ccgttaaccc ggccttgggt ggcttggctt atgaggcgtt tcagtgggct cggcagaccg cgtcgaaagc aggcagtggc ttctttagcg gtgtcccgac cttcatcaag gacaacatcg atgtcgctgg acagccgacg atgcgtggca gcgacgcatg ggtgccccga aatgcgttcg acgacggaga gttcacccgg ctgtatttgg ccaccggacc ggtatctttg ggcaagacgc agttgtcaga attcgggttc agcgcctccg ctgaacatat gcgtctaggg ccggttcgca acccatggga caccgactac actgccgggg cctcgtcttc gggatcgggt gcgttcgtcg ccgccggtgt agtcccgatg gcgcacgcca acgatggtgg cgggtcgatt cgtattccgg cctcctgcaa cgggcttgtc gggctcaagc cgtcacgcgg acgattgccg ctggattccg agctgcgccg gcttccggtg ggcatcgtag tcaacggcgt gctgacccgc tcggtgcggg acactgcggc cttctataga gaggctgagc gtatttggca caaccccaag ctgccgccgg tcggagacgt cacgcagccc ggtcggcaac ggcttcggat tgccgtggtg acgcgttcgg ttcagcgcga gtgtagcccc gaactacgcg agctcacact gaagtcggct aggctgctcg aggagctggg ccatcgcgtc gaacgggttg ccgagccccc ggtgccgcct aatttcccgg acgatttcct gctgtattgg ggactgttgg cggcgatgca ggtgcggacc ggccggctcg cgttcggcaa cacgttcgac cgcaccaagc tggacagttt gacgctgggc ctggatcgcc acgccagccg caacatgcac cggctcccca aggcaatcat gcgtttgcgt aggctgcggc gtcgcaccgc tgactttttc gctacctacg atgtattact tactccaacg gtcgctgacg agacaccgcg gatagggtac ctcactccca ccgactatca gcaggtgatg gaccggctga tgggctgggt cgctttcact ccgttgcaaa acgtcactgg ggagccggcg atttcgctgc cgttggccca atccgctgat ggtatgccgg tgggcatgat gttcaccgct gactttggtc aggaagcgca actattggaa ctggcatttg aactcgaaga ggctcggccg tgggcgcgga ttcaaattgg cgactag
Details for MyleA.00951.a.A1.GE34121
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQRVHAFGD DALGDLDAVG LADAIRAGWV SRADVIEAAI VRTEAVNPAL GGLAYEAFQW ARQTASKAGS GFFSGVPTFI KDNIDVAGQP TMRGSDAWVP RNAFDDGEFT RLYLATGPVS LGKTQLSEFG FSASAEHMRL GPVRNPWDTD YTAGASSSGS GAFVAAGVVP MAHANDGGGS IRIPASCNGL VGLKPSRGRL PLDSELRRLP VGIVVNGVLT RSVRDTAAFY REAERIWXNP KLPPVGDVTQ PGRQRLRIAV XTRSVQREXS PELRELTLXC
Validated NT Sequence
ttttntttaa ctttaagang gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcaac gtgtgcacgc tttcggtgac gacgccctcg gcgatctcga cgcggtcggt ctagccgacg ctatccgagc cggttgggtc agcagagccg atgtcatcga ggcggccatt gtgcggaccg aggccgttaa cccggccttg ggtggcttgg cttatgaggc gtttcagtgg gctcggcaga ccgcgtcgaa agcaggcagt ggcttcttta gcggtgtccc gaccttcatc aaggacaaca tcgatgtcgc tggacagccg acgatgcgtg gcagcgacgc atgggtgccc cgaaatgcgt tcgacgacgg agagttcacc cggctgtatt tggccaccgg accggtatct ttgggcaaga cgcagttgtc agaattcggg ttcagcgcct ccgctgaaca tatgcgtcta gggccggttc gcaacccatg ggacaccgac tacactgccg gggcctcgtc ttcgggatcg ggtgcgttcg tcgccgccgg tgtagtcccg atggcgcacg ccaacgatgg tggcgggtcg attcgtattc cggcctcctg caacgggctt gtcgggctca agccgtcacg cggacgattg ccgctggatt ccgagctgcg ccggcttccg gtgggcatcg tagtcaacgg cgtgctgacc cgctcggtgc gggacactgc ggccttctat agagaggctg agcgtatttg gnacaacccc aagctgccgc cggtcggaga cgtcacgcag cccggtcggc aacggcttcg gattgccgtg gngacgcgtt cggttcagcg cgagtgnagc cccgaactac gcgagctcac actgaantgc taggctgctc gagnngctgg gnnatcgcgt cnaacggntn ncgagccccc gnngccgnct anttncnnga cnattncngc tnnnntgggg acnnnnngng ncnatgcnnn nnnnnnnnnn nnngntnnna annngnnnna nnngnnnnnt tgannctngn nnnnnnnccn nnnnnnnnnn nannnncnnn nnnnnnannn nnnnnnnnnn nnnngnnnng gnnnnnnnnn nnnnttnnnn nnnnnncnan nnnnntnnnt nnngnnnnnn nnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRVHAFGD DALGDLDAVG LADAIRAGWV SRADVIEAAI VRTEAVNPAL GGLAYEAFQW ARQTASKAGS GFFSGVPTFI KDNIDVAGQP TMRGSDAWVP RNAFDDGEFT RLYLATGPVS LGKTQLSEFG FSASAEHMRL GPVRNPWDTD YTAGASSSGS GAFVAAGVVP MAHANDGGGS IRIPASCNGL VGLKPSRGRL PLDSELRRLP VGIVVNGVLT RSVRDTAAFY REAERIWHNP KLPPVGDVTQ PGRQRLRIAV VTRSVQRECS PELRELTLKS ARLLEELGHR VERVAEPPVP PNFPDDFLLY WGLLAAMQVR TGRLAFGNTF DRTKLDSLTL GLDRHASRNM HRLPKAIMRL RRLRRRTADF FATYDVLLTP TVADETPRIG YLTPTDYQQV MDRLMGWVAF TPLQNVTGEP AISLPLAQSA DGMPVGMMFT ADFGQEAQLL ELAFELEEAR PWARIQIGD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaacgtgtg cacgctttcg gtgacgacgc cctcggcgat ctcgacgcgg tcggtctagc cgacgctatc cgagccggtt gggtcagcag agccgatgtc atcgaggcgg ccattgtgcg gaccgaggcc gttaacccgg ccttgggtgg cttggcttat gaggcgtttc agtgggctcg gcagaccgcg tcgaaagcag gcagtggctt ctttagcggt gtcccgacct tcatcaagga caacatcgat gtcgctggac agccgacgat gcgtggcagc gacgcatggg tgccccgaaa tgcgttcgac gacggagagt tcacccggct gtatttggcc accggaccgg tatctttggg caagacgcag ttgtcagaat tcgggttcag cgcctccgct gaacatatgc gtctagggcc ggttcgcaac ccatgggaca ccgactacac tgccggggcc tcgtcttcgg gatcgggtgc gttcgtcgcc gccggtgtag tcccgatggc gcacgccaac gatggtggcg ggtcgattcg tattccggcc tcctgcaacg ggcttgtcgg gctcaagccg tcacgcggac gattgccgct ggattccgag ctgcgccggc ttccggtggg catcgtagtc aacggcgtgc tgacccgctc ggtgcgggac actgcggcct tctatagaga ggctgagcgt atttggcaca accccaagct gccgccggtc ggagacgtca cgcagcccgg tcggcaacgg cttcggattg ccgtggtgac gcgttcggtt cagcgcgagt gtagccccga actacgcgag ctcacactga agtcggctag gctgctcgag gagctgggcc atcgcgtcga acgggttgcc gagcccccgg tgccgcctaa tttcccggac gatttcctgc tgtattgggg actgttggcg gcgatgcagg tgcggaccgg ccggctcgcg ttcggcaaca cgttcgaccg caccaagctg gacagtttga cgctgggcct ggatcgccac gccagccgca acatgcaccg gctccccaag gcaatcatgc gtttgcgtag gctgcggcgt cgcaccgctg actttttcgc tacctacgat gtattactta ctccaacggt cgctgacgag acaccgcgga tagggtacct cactcccacc gactatcagc aggtgatgga ccggctgatg ggctgggtcg ctttcactcc gttgcaaaac gtcactgggg agccggcgat ttcgctgccg ttggcccaat ccgctgatgg tatgccggtg ggcatgatgt tcaccgctga ctttggtcag gaagcgcaac tattggaact ggcatttgaa ctcgaagagg ctcggccgtg ggcgcggatt caaattggcg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa