MyleA.00940.a

SHORT (C15) CHAIN Z-ISOPRENYL DIPHOSPHATE SYNTHASE (Z-FPP SYNTHASE) (Z-FARNESYL DIPHOSPHATE SYNTHASE) (Z-FPP SYNTHETASE) (Z-FARN

CENTER ID: MyleA.00940.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00940.a.A1.GE34123 full length 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1125
RefSeq: YP_002503156.1
UniProt: A0A0H3MPT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVRNERTLKS TDFPQLPPAP DDYPTFPDKS TWPVVFPMLP PSPDGGPRRP PQHTSKAVAP RLSAAQLPTH VAIVMDGNGR WANQRGLHRT EGHKMGEAVV IDVACGAIEL GIKWLSLYAF STENWKRSVE EVRFLMGFNR DVVRRRRENL KEMGVRIRWV GSRPRLWRSV INELAIAEHM TAGNDVITIN YCVNYGGRTE IAEATREIAR LAAAGRLNPE RITESTITRH LQRPDIPDVD LFVRTSGEQR SSNFMLWQAA YTEYIFQDKL WPDYDRRDLW AACEEYASRN RRFGSA
NT Sequence
gtggttagga acgagcgcac gctgaagtcc acggattttc cgcagctgcc ccctgcgccc gacgactacc cgacctttcc cgacaagtcg acctggccgg ttgtcttccc gatgctgccg ccatcacccg acggcgggcc gaggcgaccg ccgcagcaca cgtcaaaagc tgtcgctcca cggctctcgg ccgcccaatt gccgactcat gtcgccatcg tgatggacgg caatggccgt tgggctaacc aacgcggact gcaccgcacc gagggccaca agatgggcga agcggtggtc atcgacgttg cttgcggcgc tatcgaactc gggatcaaat ggctcagtct ctatgctttt tctactgaga actggaagcg gtctgtcgaa gaggtccgtt tcctgatggg ttttaaccgc gatgtagtgc gacggcgtcg ggagaatctc aaggagatgg gggtccggat ccgatgggta gggtcgcgac cgcgcttatg gcgcagcgtc atcaatgaat tggccattgc ggaacacatg acggcgggca acgacgtcat caccatcaat tattgtgtca actacggtgg tcgcactgaa atcgcagaag ctacaagaga aattgctcgt ctggcagctg cgggccggct caatccggag cggatcaccg agtccacgat cacacggcac ttgcagcggc cagacatccc cgatgtcgat ctcttcgtgc ggacttcggg ggagcagcgg tccagcaatt tcatgttgtg gcaggcggcg tatactgaat acattttcca ggataagttg tggcccgatt atgaccgccg tgacttgtgg gcggcgtgtg aagaatatgc gtcgcgcaat cggcgcttcg gaagcgcata g
Details for MyleA.00940.a.A1.GE34123
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVVRNERTL KSTDFPQLPP APDDYPTFPD KSTWPVVFPM LPPSPDGGPR RPPQHTSKAV APRLSAAQLP THVAIVMDGN GRWANQRGLH RTEGHKMGEA VVIDVACGAI ELGIKWLSLY AFSTENWKRS VEEVRFLMGF NRDVVRRRRE NLKEMGVRIR WVGSRPRLWR SVINELAIAE HMTAGNDVIT INYCVNYGGR TEIAEATREI ARLAAAGRLN PERITESTIT RHLQRPDIPD VDLFVRTSGE QRSXNFMLWX XAYTXYIFQD KLWPDYDRRD LWAACEEXAS RNXXFXSX
Validated NT Sequence
annanttttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggttagg aacgagcgca cgctgaagtc cacggatttt ccgcagctgc cccctgcgcc cgacgactac ccgacctttc ccgacaagtc gacctggccg gttgtcttcc cgatgctgcc gccatcaccc gacggcgggc cgaggcgacc gccgcagcac acgtcaaaag ctgtcgctcc acggctctcg gccgcccaat tgccgactca tgtcgccatc gtgatggacg gcaatggccg ttgggctaac caacgcggac tgcaccgcac cgagggccac aagatgggcg aagcggtggt catcgacgtt gcttgcggcg ctatcgaact cgggatcaaa tggctcagtc tctatgcttt ttctactgag aactggaagc ggtctgtcga agaggtccgt ttcctgatgg gttttaaccg cgatgtagtg cgacggcgtc gggagaatct caaggagatg ggggtccgga tccgatgggt agggtcgcga ccgcgcttat ggcgcagcgt catcaatgaa ttggccattg cggaacacat gacggcgggc aacgacgtca tcaccatcaa ttattgtgtc aactacggtg gtcgcactga aatcgcagaa gctacaagag aaattgctcg tctggcagct gcgggccggc tcaatccgga gcggatcacc gagtccacga tcacacggca cttgcagcgg ccagacatcc ccgatgtcga tctcttcgtg cggacttcgg gggagcagcg gtccngcaat ttcatgttgt ggcnggnggc gtatactgna tacattttcc aggataagtt gtggcccgat tatgaccgcc gtgacttgtg ggcggcgtgt gaagaanatg cntcgcgcaa tngnngcttc ngaagcncat aataaacagc acgaacnagt nctgcagcca agcttnncnn ggnccngctg ctnnnannnn aannnctnan tnnngnnnnn nnnnnnnnnn nnnnnnnntt nnnnnnnnnn nnnnnnnnnn annnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVVRNERTL KSTDFPQLPP APDDYPTFPD KSTWPVVFPM LPPSPDGGPR RPPQHTSKAV APRLSAAQLP THVAIVMDGN GRWANQRGLH RTEGHKMGEA VVIDVACGAI ELGIKWLSLY AFSTENWKRS VEEVRFLMGF NRDVVRRRRE NLKEMGVRIR WVGSRPRLWR SVINELAIAE HMTAGNDVIT INYCVNYGGR TEIAEATREI ARLAAAGRLN PERITESTIT RHLQRPDIPD VDLFVRTSGE QRSSNFMLWQ AAYTEYIFQD KLWPDYDRRD LWAACEEYAS RNRRFGSA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggttagg aacgagcgca cgctgaagtc cacggatttt ccgcagctgc cccctgcgcc cgacgactac ccgacctttc ccgacaagtc gacctggccg gttgtcttcc cgatgctgcc gccatcaccc gacggcgggc cgaggcgacc gccgcagcac acgtcaaaag ctgtcgctcc acggctctcg gccgcccaat tgccgactca tgtcgccatc gtgatggacg gcaatggccg ttgggctaac caacgcggac tgcaccgcac cgagggccac aagatgggcg aagcggtggt catcgacgtt gcttgcggcg ctatcgaact cgggatcaaa tggctcagtc tctatgcttt ttctactgag aactggaagc ggtctgtcga agaggtccgt ttcctgatgg gttttaaccg cgatgtagtg cgacggcgtc gggagaatct caaggagatg ggggtccgga tccgatgggt agggtcgcga ccgcgcttat ggcgcagcgt catcaatgaa ttggccattg cggaacacat gacggcgggc aacgacgtca tcaccatcaa ttattgtgtc aactacggtg gtcgcactga aatcgcagaa gctacaagag aaattgctcg tctggcagct gcgggccggc tcaatccgga gcggatcacc gagtccacga tcacacggca cttgcagcgg ccagacatcc ccgatgtcga tctcttcgtg cggacttcgg gggagcagcg gtccagcaat ttcatgttgt ggcaggcggc gtatactgaa tacattttcc aggataagtt gtggcccgat tatgaccgcc gtgacttgtg ggcggcgtgt gaagaatatg cgtcgcgcaa tcggcgcttc ggaagcgcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa