MyleA.00896.a

Citrate synthase

CENTER ID: MyleA.00896.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00896.a.A1.GE29241 Full length( MyleA.00896.a ) 1 431
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.4045
RefSeq: YP_002504035.1
UniProt: A0A0H3N0I1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MADTDDTVTL KYPGGEIDLQ IVRATEGNDG IAIVPVLAKT GYTTFDVGYG NTASTKSSIT YIDGDAGILR YRGYPIEQLA EKSTFIEVCY LLIYGELPAT DQLAEFTHRI QRHTMLHEDL KRFYDGFPRN AHPMPVLSSV VNALSAYYQD ALDPMDNGQV ELSTIRLLAK LPTIAAYAYK KSVGQPFLYP DNAFSLVENF LRMTFGLPAE PYEPDPEVVR ALDMLFILHA DHEQNCSTST VRLVGSSRAN LFTSISGGIN ALWGPLHGGA NQAVLEMLEG IRESGDDVGG FVQKVKNCQA GVKLMGFGHR VYKNYDPRAR IVKEQADKIL VKLGGDDDLL NIAKGLEEAA LTDDYFIERK LYPNVDFYTG LIYRALGFPT RMFTVLFALG RLPGWIAHWR EMHDEGDSKI GRPRQIYTGY TERDYTAIET R
NT Sequence
GTGGCCGACA CCGACGACAC CGTCACTCTG AAGTATCCGG GAGGCGAGAT CGATCTACAG ATTGTCCGTG CCACCGAAGG TAATGACGGC ATTGCGATCG TCCCGGTATT GGCCAAGACC GGTTACACTA CGTTTGATGT CGGCTACGGT AACACCGCAT CCACCAAGAG TTCCATCACC TACATTGATG GTGACGCCGG GATTCTGCGT TACCGCGGCT ATCCGATTGA GCAGCTCGCC GAGAAGTCGA CCTTCATAGA GGTTTGCTAT CTGCTGATCT ATGGGGAGTT ACCAGCCACC GATCAGCTGG CCGAATTCAC CCATCGGATC CAGCGCCACA CCATGTTGCA CGAGGACCTT AAGCGATTCT ATGATGGCTT CCCGCGCAAC GCGCACCCGA TGCCGGTGTT GTCCAGCGTG GTCAACGCAT TGAGTGCTTA CTATCAGGAT GCACTGGACC CGATGGACAA CGGCCAAGTA GAGCTGTCGA CGATCCGACT CTTGGCCAAG CTGCCCACTA TCGCTGCCTA TGCATACAAG AAGTCGGTAG GCCAGCCGTT CCTTTACCCG GACAACGCGT TTTCTCTAGT AGAGAACTTC CTTCGAATGA CTTTTGGTTT GCCGGCTGAG CCCTATGAGC CCGACCCTGA GGTGGTCCGG GCTCTGGACA TGCTGTTTAT CCTGCATGCT GACCACGAGC AAAACTGTTC GACGTCAACC GTGCGTTTGG TGGGCTCGTC GCGAGCCAAC CTGTTCACCT CGATTTCGGG CGGCATTAAC GCGCTGTGGG GACCGCTGCA CGGCGGTGCC AACCAGGCGG TGCTTGAGAT GCTCGAAGGT ATCCGCGAGA GTGGCGACGA CGTTGGTGGA TTTGTCCAAA AAGTAAAGAA TTGCCAGGCC GGTGTGAAAT TGATGGGCTT TGGTCACCGC GTCTACAAGA ACTACGATCC GCGGGCCCGC ATCGTCAAGG AACAGGCTGA CAAGATCCTG GTCAAACTTG GTGGCGATGA CGATTTGCTG AACATTGCCA AGGGACTCGA GGAGGCGGCG CTAACCGATG ACTATTTCAT CGAGCGCAAG CTTTACCCGA ACGTCGACTT CTACACCGGT CTGATCTACC GGGCCCTCGG TTTTCCGACC CGGATGTTCA CCGTGTTGTT TGCTTTGGGT CGGCTGCCAG GCTGGATTGC GCACTGGCGT GAGATGCACG ACGAGGGTGA CAGTAAGATT GGCCGTCCGC GCCAAATATA CACCGGCTAC ACAGAGCGTG ACTACACCGC AATTGAAACA CGA
Details for MyleA.00896.a.A1.GE29241
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 50kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MXHHHHHHMG TLEAQTQGPG SMVADTDDTV TLKYPGGEID LQIVRATEGN DGIAIVPVLA KTGYTTFDVG YGNTASTKSS ITYIDGDAGI LRYRGYPIEQ LAEKSTFIEV CYLLIYGELP ATDQLAEFTH RIQRHTMLHE DLKRFYDGFP RNAHPMPVLS SVVNALSAYY QDALDPMDNG QVELSTIRLL AKLPTIAAYA YKKSVGQPFL YPDNAFSLVE NFLRMTFGLP AEPYEPDPEV VRALDMLFIL HADHEQNCST STVRLVGSSR ANLFTSISGG INALWGPLHG GANQAVLEML EGIRESGDDV GGFVQKVKNC QAGVKLMGFG HRVYKNYDPR ARIVKEQADK ILVKLGGDDD LLNIAKGLEE AALTDDYFIE RKLYPNVDFY TGLIYRALGF PTRMFTVLFA LGRLPGWIAH WREMHDEGDS KIGRPRQIYT GYTERDYTAI ET
Validated NT Sequence
gtgtttcaat tgcggtgtag tcacgctctg tgtagccggt gtatatttgg cgcggacggc caatcttact gtcaccctcg tcgtgcatct cacgccagtg cgcaatccag cctggcagcc gacccaaagc aaacaacacg gtgaacatcc gggtcggaaa accgagggcc cggtagatca gaccggtgta gaagtcgacg ttcgggtaaa gcttgcgctc gatgaaatag tcatcggtta gcgccgcctc ctcgagtccc ttggcaatgt tcagcaaatc gtcatcgcca ccaagtttga ccaggatctt gtcagcctgt tccttgacga tgcgggcccg cggatcgtag ttcttgtaga cgcggtgacc aaagcccatc aatttcacac cggcctggca attctttact ttttggacaa atccaccaac gtcgtcgcca ctctcgcgga taccttcgag catctcaagc accgcctggt tggcaccgcc gtgcagcggt ccccacagcg cgttaatgcc gcccgaaatc gaggtgaaca ggttggctcg cgacgagccc accaaacgca cggttgacgt cgaacagttt tgctcgtggt cagcatgcag gataaacagc atgtccagag cccggaccac ctcagggtcg ggctcatagg gctcagccgg caaaccaaaa gtcattcgaa ggaagttctc tactagagaa aacgcgttgt ccgggtaaag gaacggctgg cctaccgact tcttgtatgc ataggcagcg atagtgggca gcttggccaa gagtcggatc gtcgacagct ctacttggcc gttgtccatc gggtccagtg catcctgata gtaagcactc aatgcgttga ccacgctgga caacaccggc atcgggtgcg cgttgcgcgg gaagccatca tagaatcgct taaggtcctc gtgcaacatg gtgtggcgct ggatccgatg ggtgaattcg gccagctgat cggtggctgg taactcccca tagatcagca gatagcaaac ctctatgaag gtcgacttct cggcgagctg ctcaatcgga tagccgcggt aacgcagaat cccggcgtca ccatcaatgt aggtgatgga actcttggtg gatgcggtgt taccgtagcc gacatcaaac gtagtgtaac cggtcttggc caataccggg acgatcgcaa tgccgtcatt accttcggtg gcacggacaa tctgtagatc gatctcgcct cccggatact tcagagtgac ggtgtcgtcg gtgtcggcca ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tgatggtgat ganccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVADTDDTV TLKYPGGEID LQIVRATEGN DGIAIVPVLA KTGYTTFDVG YGNTASTKSS ITYIDGDAGI LRYRGYPIEQ LAEKSTFIEV CYLLIYGELP ATDQLAEFTH RIQRHTMLHE DLKRFYDGFP RNAHPMPVLS SVVNALSAYY QDALDPMDNG QVELSTIRLL AKLPTIAAYA YKKSVGQPFL YPDNAFSLVE NFLRMTFGLP AEPYEPDPEV VRALDMLFIL HADHEQNCST STVRLVGSSR ANLFTSISGG INALWGPLHG GANQAVLEML EGIRESGDDV GGFVQKVKNC QAGVKLMGFG HRVYKNYDPR ARIVKEQADK ILVKLGGDDD LLNIAKGLEE AALTDDYFIE RKLYPNVDFY TGLIYRALGF PTRMFTVLFA LGRLPGWIAH WREMHDEGDS KIGRPRQIYT GYTERDYTAI ETR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccgac accgacgaca ccgtcactct gaagtatccg ggaggcgaga tcgatctaca gattgtccgt gccaccgaag gtaatgacgg cattgcgatc gtcccggtat tggccaagac cggttacact acgtttgatg tcggctacgg taacaccgca tccaccaaga gttccatcac ctacattgat ggtgacgccg ggattctgcg ttaccgcggc tatccgattg agcagctcgc cgagaagtcg accttcatag aggtttgcta tctgctgatc tatggggagt taccagccac cgatcagctg gccgaattca cccatcggat ccagcgccac accatgttgc acgaggacct taagcgattc tatgatggct tcccgcgcaa cgcgcacccg atgccggtgt tgtccagcgt ggtcaacgca ttgagtgctt actatcagga tgcactggac ccgatggaca acggccaagt agagctgtcg acgatccgac tcttggccaa gctgcccact atcgctgcct atgcatacaa gaagtcggta ggccagccgt tcctttaccc ggacaacgcg ttttctctag tagagaactt ccttcgaatg acttttggtt tgccggctga gccctatgag cccgaccctg aggtggtccg ggctctggac atgctgttta tcctgcatgc tgaccacgag caaaactgtt cgacgtcaac cgtgcgtttg gtgggctcgt cgcgagccaa cctgttcacc tcgatttcgg gcggcattaa cgcgctgtgg ggaccgctgc acggcggtgc caaccaggcg gtgcttgaga tgctcgaagg tatccgcgag agtggcgacg acgttggtgg atttgtccaa aaagtaaaga attgccaggc cggtgtgaaa ttgatgggct ttggtcaccg cgtctacaag aactacgatc cgcgggcccg catcgtcaag gaacaggctg acaagatcct ggtcaaactt ggtggcgatg acgatttgct gaacattgcc aagggactcg aggaggcggc gctaaccgat gactatttca tcgagcgcaa gctttacccg aacgtcgact tctacaccgg tctgatctac cgggccctcg gttttccgac ccggatgttc accgtgttgt ttgctttggg tcggctgcca ggctggattg cgcactggcg tgagatgcac gacgagggtg acagtaagat tggccgtccg cgccaaatat acaccggcta cacagagcgt gactacaccg caattgaaac acgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa