MyleA.00778.a

Putative molybdenum cofactor biosynthesis protein

CENTER ID: MyleA.00778.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: native diffraction-data
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00778.a.A1.GE29238 Full length( MyleA.00778.a ) 1 181
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_130343
PATRIC ID: fig|561304.4.peg.279
RefSeq: YP_002502884.1
UniProt: A0A0H3MPA6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLVDKQLSEL GYTVAPMEKG VELVVGRALI VVVDDRTAHG DEDHSGPLVT ELLTEAGFVV DGVVAVAADE VEIRNALNTA VIGGVDLVVS VGGTGVTPRD VAPEATREIL DREILGIAEA IRASGLSAGI TDAGLSRGLA GVSGSTLVVN LAGSRYAVRD GMATLNPLAT QIIGQLSSLE I
NT Sequence
GTGTTAGTCG ATAAGCAATT GTCCGAGCTC GGATATACGG TTGCACCCAT GGAGAAGGGT GTCGAGTTGG TAGTCGGACG TGCACTTATT GTCGTTGTCG ACGATCGCAC CGCGCATGGG GATGAGGACC ACAGCGGGCC GCTGGTCACT GAGCTGTTGA CCGAGGCCGG GTTCGTCGTT GATGGTGTGG TGGCGGTGGC GGCTGACGAG GTCGAGATAC GCAACGCGTT GAACACTGCA GTGATCGGTG GGGTGGACCT AGTGGTCTCG GTCGGCGGAA CCGGTGTTAC ACCACGTGAC GTCGCTCCAG AGGCCACCCG CGAGATTCTG GATCGGGAAA TTCTAGGTAT TGCCGAAGCC ATCCGTGCTT CGGGGCTGTC CGCGGGAATC ACCGACGCCG GATTGTCGCG TGGTCTAGCT GGTGTTTCGG GCAGTACGTT GGTGGTAAAC CTCGCCGGCT CCCGTTATGC GGTGCGCGAC GGAATGGCTA CATTGAATCC GCTGGCCACC CAGATCATTG GGCAATTATC GAGTCTGGAA ATC
Details for MyleA.00778.a.A1.GE29238
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 20kDa
OBSERVED MW: 21kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVLVDKQLS ELGYTVAPME KGVELVVGRA LIVVVDDRTA HGDEDHSGPL VTELLTEAGF VVDGVVAVAA DEVEIRNALN TAVIGGVDLV VSVGGTGVTP RDVAPEATRE ILDREILGIA EAIRASGLSA GITDAGLSRG LAGVSGSTLV VNLAGSRYAV RDGMATLNPL ATQIIGQLSS L
Validated NT Sequence
ccagactcga taattgccca atgatctggg tggccagcgg attcaatgta gccattccgt cgcgcaccgc ataacgggag ccggcgaggt ttaccaccaa cgtactgccc gaaacaccag ctagaccacg cgacaatccg gcgtcggtga ttcccgcgga cagccccgaa gcacggatgg cttcggcaat acctagaatt tcccgatcca gaatctcgcg ggtggcctct ggagcgacgt cacgtggtgt aacaccggtt ccgccgaccg agaccactag gtccacccca ccgatcactg cagtgttcaa cgcgttgcgt atctcgacct cgtcagccgc caccgccacc acaccatcaa cgacgaaccc ggcctcggtc aacagctcag tgaccagcgg cccgctgtgg tcctcatccc catgcgcggt gcgatcgtcg acaacgacaa taagtgcacg tccgactacc aactcgacac ccttctccat gggtgcaacc gtatatccga gctcggacaa ttgcttatcg actaacacca tcgaaccagg accctgggtc tgagcttcca gggtacccat atgatggtga tggtgatgag ccat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLVDKQLS ELGYTVAPME KGVELVVGRA LIVVVDDRTA HGDEDHSGPL VTELLTEAGF VVDGVVAVAA DEVEIRNALN TAVIGGVDLV VSVGGTGVTP RDVAPEATRE ILDREILGIA EAIRASGLSA GITDAGLSRG LAGVSGSTLV VNLAGSRYAV RDGMATLNPL ATQIIGQLSS LEI
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgttagtc gataagcaat tgtccgagct cggatatacg gttgcaccca tggagaaggg tgtcgagttg gtagtcggac gtgcacttat tgtcgttgtc gacgatcgca ccgcgcatgg ggatgaggac cacagcgggc cgctggtcac tgagctgttg accgaggccg ggttcgtcgt tgatggtgtg gtggcggtgg cggctgacga ggtcgagata cgcaacgcgt tgaacactgc agtgatcggt ggggtggacc tagtggtctc ggtcggcgga accggtgtta caccacgtga cgtcgctcca gaggccaccc gcgagattct ggatcgggaa attctaggta ttgccgaagc catccgtgct tcggggctgt ccgcgggaat caccgacgcc ggattgtcgc gtggtctagc tggtgtttcg ggcagtacgt tggtggtaaa cctcgccggc tcccgttatg cggtgcgcga cggaatggct acattgaatc cgctggccac ccagatcatt gggcaattat cgagtctgga aatcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa