MyleA.00771.a

PROBABLE DIAMINOPIMELATE DECARBOXYLASE LYSA (DAP DECARBOXYLASE)

CENTER ID: MyleA.00771.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00771.a.A1.GE34127 full length 1 472
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2055
RefSeq: YP_002503452.1
UniProt: A0A0H3MQP9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNVHTAGPRH AEKTRHTATP QRVQPSDDLL RLASNVWPRN ITRDETGVAC IAGNKLTDLA GEYGTPLFVI DEDDFRFRCR EIAAAFGGGE NVHYAAKAFL CTEIARWIDE EGLSLDVCSG GELAVALHAS FPPERISLHG NNKSVAELKD AVKAGVGYIV LDSTTEIERL DAIAGEAGIV QDVLVRLTVG VEAHTHEFIA TAHEDQKFGL SVASGAAMAA VRRVFATDNL RLVGLHSHIG SQIFDVAGFE LAAHRVIGLL CDIVGEFDPE KTAQLSIVDL GGGLGISYLP DDDPPPIFEL AAKLGAIVSN ESAAVGLPVP KLMVEPGRAI AGPGTITLYE VGTIKDVDVS ATAHRRYVSI DGGMSDNIRT ALYDAQYDVR LVSRTSDAPA APASIVGKHC ESGDIVVRDT WVPDDLKPGD LVGVAATGAY CYSLSSRYNM LGRPAVVAVC AGQARLILRR ETVDDLLSLE VR
NT Sequence
gtgaacgtac ataccgccgg tcctcggcac gctgaaaaaa cccgtcacac cgcgactcca cagcgtgtgc aaccctcaga tgacctgttg cgattagcgt caaatgtctg gccgcgtaac attactcgag atgaaaccgg agttgcttgc attgcgggaa ataaactgac cgaccttgcc ggggagtatg ggactccgtt gtttgtcatc gacgaagacg acttccgttt ccgttgccgg gagatagcgg ctgcattcgg cggtggggaa aatgtgcact atgccgccaa ggccttcttg tgtaccgaga tagctcggtg gatcgacgaa gaaggtcttt ctctcgacgt gtgcagcggc ggtgagttgg ccgttgcgct gcatgcttct tttccaccag agcgtattag cttgcacggt aacaacaaat cggtagcaga gttgaaagat gcagtcaaag ccggagtggg ctatatcgtt ttagactcga cgaccgaaat cgagcgcctc gatgccatcg cgggcgaagc gggcatcgtc caagatgtct tggtgcgcct gactgtcggt gtagaggcgc acactcatga gttcatcgcc actgcacatg aagatcaaaa attcgggctg tcggttgcca gcggtgcggc gatggctgcg gtccgccggg tattcgcaac cgataatctg cgactggttg ggctgcacag ccacatcggt tcgcagatct tcgatgtcgc cggcttcgaa cttgctgcac accgtgtcat cggcctactg tgcgatattg tcggcgagtt tgatccggag aagacagccc agctatcgat cgtcgacctc ggtggtggcc tggggatctc gtacttgccg gatgacgacc caccgccgat attcgagctg gcggccaagc tgggcgctat cgtgagcaac gagtcagccg ccgtggggct gccggtgcct aagttgatgg tcgaacccgg acgcgccatc gccggaccgg gcaccatcac gttgtatgag gtcggcacta ttaaggacgt cgacgtgagt gctaccgcgc accggcgcta cgtcagcatc gatgggggga tgagcgacaa catccgcaca gcgctttacg acgcgcaata tgacgtccga cttgtctcga gaactagcga tgcgccagcg gcacctgcca gcatcgtcgg aaagcattgc gaaagtggcg atatcgttgt acgtgacacc tgggttcctg acgacttgaa acccggcgac ctggttgggg tggccgcaac cggggcctac tgctattcgt tgtcaagtcg ttacaacatg ctcggccgcc ccgctgtggt tgcggtgtgc gcggggcaag ctcgtctgat cctgcgccgg gagacggttg acgatctgct gagtctggaa gtgaggtga
Details for MyleA.00771.a.A1.GE34127
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 79
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNVHTAGP RHAEKTRHTA TPQRVQPSDD LLRLASNVWP RNITRDETGV ACIAGNKLTD LAGEYGTPLF VIDEDDFRFR CREIAAAFGG GENVHYAAKA FLCTEIARWI DEEGLSLDVC SGGELAVALH ASFPPERISL HGNNKSVAEL KDAVKAGVGY IVLDSTTEIE RLDAIAGEAG IVQDVLVRLT VGVEAHTHEF IATAHEDQKF GLSVASGAAM AAVRRVFATD NLRLVGLHSH IGSQIFDVAG FELAAHRVIG LLCXIVGXFX PEXQPSYRSX TRWWLGXXYL XXRPXXXSSX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
ttttgtnnnn tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggtgaa cgtacatacc gccggtcctc ggcacgctga aaaaacccgt cacaccgcga ctccacagcg tgtgcaaccc tcagatgacc tgttgcgatt agcgtcaaat gtctggccgc gtaacattac tcgagatgaa accggagttg cttgcattgc gggaaataaa ctgaccgacc ttgccgggga gtatgggact ccgttgtttg tcatcgacga agacgacttc cgtttccgtt gccgggagat agcggctgca ttcggcggtg gggaaaatgt gcactatgcc gccaaggcct tcttgtgtac cgagatagct cggtggatcg acgaagaagg tctttctctc gacgtgtgca gcggcggtga gttggccgtt gcgctgcatg cttcttttcc accagagcgt attagcttgc acggtaacaa caaatcggta gcagagttga aagatgcagt caaagccgga gtgggctata tcgttttaga ctcgacgacc gaaatcgagc gcctcgatgc catcgcgggc gaagcgggca tcgtccaaga tgtcttggtg cgcctgactg tcggtgtaga ggcgcacact catgagttca tcgccactgc acatgaagat caaaaattcg ggctgtcggt tgccagcggt gcggcgatgg ctgcggtccg ccgggtattc gcaaccgata atctgcgact ggttgggctg cacagccaca tcggttcgca gatcttcgat gtcgccggct tcgaacttgc tgcacaccgt gtcatcggcc tactgtgcga nattgtcggc nagtttnatc cngaganaca gccnagctat cgatcgncna cncggtggtg gctggggntc tngtacttgn nnngacgacc cnnncnannt tcnagcngnn nnncngnnnc tntcnngagn annantcanc ncnngnnntn cncnantnnn gnncnanncn nnnnnnnnnn nnccnncnnn nnnnncntna nnnnnnnnnn nnnnnnnnan nnnnnnnnnn cnnnnnnggn ntnnnnggnn nannaaannn nnnnnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNVHTAGP RHAEKTRHTA TPQRVQPSDD LLRLASNVWP RNITRDETGV ACIAGNKLTD LAGEYGTPLF VIDEDDFRFR CREIAAAFGG GENVHYAAKA FLCTEIARWI DEEGLSLDVC SGGELAVALH ASFPPERISL HGNNKSVAEL KDAVKAGVGY IVLDSTTEIE RLDAIAGEAG IVQDVLVRLT VGVEAHTHEF IATAHEDQKF GLSVASGAAM AAVRRVFATD NLRLVGLHSH IGSQIFDVAG FELAAHRVIG LLCDIVGEFD PEKTAQLSIV DLGGGLGISY LPDDDPPPIF ELAAKLGAIV SNESAAVGLP VPKLMVEPGR AIAGPGTITL YEVGTIKDVD VSATAHRRYV SIDGGMSDNI RTALYDAQYD VRLVSRTSDA PAAPASIVGK HCESGDIVVR DTWVPDDLKP GDLVGVAATG AYCYSLSSRY NMLGRPAVVA VCAGQARLIL RRETVDDLLS LEVR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaacgta cataccgccg gtcctcggca cgctgaaaaa acccgtcaca ccgcgactcc acagcgtgtg caaccctcag atgacctgtt gcgattagcg tcaaatgtct ggccgcgtaa cattactcga gatgaaaccg gagttgcttg cattgcggga aataaactga ccgaccttgc cggggagtat gggactccgt tgtttgtcat cgacgaagac gacttccgtt tccgttgccg ggagatagcg gctgcattcg gcggtgggga aaatgtgcac tatgccgcca aggccttctt gtgtaccgag atagctcggt ggatcgacga agaaggtctt tctctcgacg tgtgcagcgg cggtgagttg gccgttgcgc tgcatgcttc ttttccacca gagcgtatta gcttgcacgg taacaacaaa tcggtagcag agttgaaaga tgcagtcaaa gccggagtgg gctatatcgt tttagactcg acgaccgaaa tcgagcgcct cgatgccatc gcgggcgaag cgggcatcgt ccaagatgtc ttggtgcgcc tgactgtcgg tgtagaggcg cacactcatg agttcatcgc cactgcacat gaagatcaaa aattcgggct gtcggttgcc agcggtgcgg cgatggctgc ggtccgccgg gtattcgcaa ccgataatct gcgactggtt gggctgcaca gccacatcgg ttcgcagatc ttcgatgtcg ccggcttcga acttgctgca caccgtgtca tcggcctact gtgcgatatt gtcggcgagt ttgatccgga gaagacagcc cagctatcga tcgtcgacct cggtggtggc ctggggatct cgtacttgcc ggatgacgac ccaccgccga tattcgagct ggcggccaag ctgggcgcta tcgtgagcaa cgagtcagcc gccgtggggc tgccggtgcc taagttgatg gtcgaacccg gacgcgccat cgccggaccg ggcaccatca cgttgtatga ggtcggcact attaaggacg tcgacgtgag tgctaccgcg caccggcgct acgtcagcat cgatgggggg atgagcgaca acatccgcac agcgctttac gacgcgcaat atgacgtccg acttgtctcg agaactagcg atgcgccagc ggcacctgcc agcatcgtcg gaaagcattg cgaaagtggc gatatcgttg tacgtgacac ctgggttcct gacgacttga aacccggcga cctggttggg gtggccgcaa ccggggccta ctgctattcg ttgtcaagtc gttacaacat gctcggccgc cccgctgtgg ttgcggtgtg cgcggggcaa gctcgtctga tcctgcgccg ggagacggtt gacgatctgc tgagtctgga agtgaggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa