MyleA.00753.a

Probable binding-protein-dependent transport protein

CENTER ID: MyleA.00753.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00753.a.A1.GE34934 Full length( MyleA.00753.a ) 1 356
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2649
RefSeq: YP_002503642.1
UniProt: A0A0H3MZQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASVTFEQAT RQFPDTDRPA LDRLDLDVDD GEFVVVVGPS GCGKTTSLRM VAGLETLDSG CIRIGDRDVT HVDPKDRDVA MVFQNYALYP HMTVAQNLGF ALKISKISKA EIHDRILVVA KLLDLQPYLD RKPKDLSGGQ RQRVAMGRAI VRRPQVFLMD EPLSNLDAKL RAQTRNQIAE LQCQLGTTTV YVTHDQVEAM TMGDRVAVLC YGVLQQFATP RELYHKPENV FVAGFIGSPG MNLVTLSIVD SSVLLGDWPI RIPREIASAA SEVIIGVRPE HFELGSLGVE VEIDMVEELG ADAYLYGRIA GASKVTDQLV VARVDGRNPP AKGSRVRLYT EPGNVHFFGV DGHRIS
NT Sequence
ATGGCTTCGG TGACCTTCGA ACAAGCGACA CGCCAGTTCC CTGACACAGA TCGACCAGCC CTGGATCGCC TCGACCTCGA CGTCGATGAT GGCGAATTTG TGGTTGTGGT AGGGCCATCT GGATGCGGCA AGACCACCTC GCTGCGGATG GTGGCCGGAT TAGAAACGCT GGATTCCGGA TGTATTCGGA TCGGCGACCG CGATGTCACT CACGTCGATC CCAAGGATCG TGACGTCGCG ATGGTCTTTC AAAACTATGC CCTTTACCCG CACATGACCG TAGCGCAGAA TCTAGGGTTT GCGTTAAAGA TCTCTAAAAT CTCTAAGGCC GAGATCCACG ATCGGATACT CGTCGTTGCG AAACTACTTG ATCTGCAACC GTATCTGGAT CGCAAACCAA AAGATCTTTC CGGAGGTCAA CGTCAGCGGG TAGCGATGGG GCGGGCCATC GTGCGCCGTC CACAGGTGTT CTTGATGGAT GAGCCGCTGT CCAATCTCGA TGCCAAACTG CGAGCGCAAA CTCGTAATCA AATCGCGGAA TTGCAGTGTC AACTCGGCAC TACCACCGTC TATGTCACCC ACGACCAGGT CGAAGCCATG ACCATGGGGG ACCGTGTCGC GGTACTGTGC TACGGGGTGT TACAACAGTT CGCTACGCCT CGTGAACTTT ATCACAAACC CGAGAATGTT TTTGTCGCAG GATTCATAGG GTCTCCCGGA ATGAACTTGG TCACCCTCTC GATCGTCGAT TCCTCTGTTT TGCTGGGGGA TTGGCCGATT CGCATACCGC GTGAAATCGC CAGCGCCGCA AGCGAGGTCA TCATCGGCGT CCGTCCCGAA CATTTCGAGC TGGGCAGCCT CGGTGTCGAA GTGGAGATCG ACATGGTCGA AGAGCTCGGG GCCGATGCTT ATCTATATGG CCGAATCGCA GGTGCCAGCA AAGTAACCGA CCAACTTGTC GTTGCACGCG TCGACGGCCG AAACCCGCCG GCAAAGGGCA GCCGCGTTCG TCTGTATACA GAACCCGGGA ATGTGCATTT CTTCGGCGTT GATGGCCATC GAATTTCT
Details for MyleA.00753.a.A1.GE34934
HARVESTED ON: 4/17/2012
SEQUENCED ON: 4/17/2012
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 88
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASVTFEQA TRQFPDTDRP ALDRLDLDVD DGEFVVVVGP SGCGKTTSLR MVAGLETLDS GCIRIGDRDV THVDPKDRDV AMVFQNYALY PHMTVAQNLG FALKISKISK AEIHDRILVV AKLLDLQPYL DRKPKDLSGG QRQRVAMGRA IVRRPQVFLM DEPLSNLDAK LRAQTRNQIA ELQCQLGTTT VYVTHDQVEA MTMGXRVAVL CYGVLQQFAT PRELYHKPEN XFVAXXIGSP GMNLVTLSIV DSSVLLGXWP IRIPREIASA ASEXIIGXXP XHFELGSXGX XXXSTWSXXX XXAXLXGXIX XXXK
Validated NT Sequence
anattttntt tnnctttaag aaggaganat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatgg cttcggtgac cttcgaacaa gcgacacgcc agttccctga cacagatcga ccagccctgg atcgcctcga cctcgacgtc gatgatggcg aatttgtggt tgtggtaggg ccatctggat gcggcaagac cacctcgctg cggatggtgg ccggattaga aacgctggat tccggatgta ttcggatcgg cgaccgcgat gtcactcacg tcgatcccaa ggatcgtgac gtcgcgatgg tctttcaaaa ctatgccctt tacccgcaca tgaccgtagc gcagaatcta gggtttgcgt taaagatctc taaaatctct aaggccgaga tccacgatcg gatactcgtc gttgcgaaac tacttgatct gcaaccgtat ctggatcgca aaccaaaaga tctttccgga ggtcaacgtc agcgggtagc gatggggcgg gccatcgtgc gccgtccaca ggtgttcttg atggatgagc cgctgtccaa tctcgatgcc aaactgcgag cgcaaactcg taatcaaatc gcggaattgc agtgtcaact cggcactacc accgtctatg tcacccacga ccaggtcgaa gccatgacca tgggggancg tgtcgcggta ctgtgctacg gggtgttaca acagttcgct acgcctcgtg aactttatca caaacccgag aatgnttttg tcgcnnnant catagggtct cccggaatga acttggtcac cctctcgatc gtcgattcct ctgttttgct gggggantgg ccgattcgca taccgcgtga aatcgccagc gccgcnagcg aggncatcat cggcntccnt ccnnaacatt tcgagctggg cagcntcggt ntcnaantnn natcnacatg gtcgaanntc ngggncnang ctnatctatn tggncnaatc ncnnngncag nnaagtaacc nannnactng ncnttgcacg cgtcgannnn gaanncnnnn nnnagnnnnc nnnntnngnn ngnnnnnnnn nngnangngc ntnnntnnnn tgncntcnat tntnnnnnnn nnnannantn nnnnnca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASVTFEQA TRQFPDTDRP ALDRLDLDVD DGEFVVVVGP SGCGKTTSLR MVAGLETLDS GCIRIGDRDV THVDPKDRDV AMVFQNYALY PHMTVAQNLG FALKISKISK AEIHDRILVV AKLLDLQPYL DRKPKDLSGG QRQRVAMGRA IVRRPQVFLM DEPLSNLDAK LRAQTRNQIA ELQCQLGTTT VYVTHDQVEA MTMGDRVAVL CYGVLQQFAT PRELYHKPEN VFVAGFIGSP GMNLVTLSIV DSSVLLGDWP IRIPREIASA ASEVIIGVRP EHFELGSLGV EVEIDMVEEL GADAYLYGRI AGASKVTDQL VVARVDGRNP PAKGSRVRLY TEPGNVHFFG VDGHRIS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcttcggtg accttcgaac aagcgacacg ccagttccct gacacagatc gaccagccct ggatcgcctc gacctcgacg tcgatgatgg cgaatttgtg gttgtggtag ggccatctgg atgcggcaag accacctcgc tgcggatggt ggccggatta gaaacgctgg attccggatg tattcggatc ggcgaccgcg atgtcactca cgtcgatccc aaggatcgtg acgtcgcgat ggtctttcaa aactatgccc tttacccgca catgaccgta gcgcagaatc tagggtttgc gttaaagatc tctaaaatct ctaaggccga gatccacgat cggatactcg tcgttgcgaa actacttgat ctgcaaccgt atctggatcg caaaccaaaa gatctttccg gaggtcaacg tcagcgggta gcgatggggc gggccatcgt gcgccgtcca caggtgttct tgatggatga gccgctgtcc aatctcgatg ccaaactgcg agcgcaaact cgtaatcaaa tcgcggaatt gcagtgtcaa ctcggcacta ccaccgtcta tgtcacccac gaccaggtcg aagccatgac catgggggac cgtgtcgcgg tactgtgcta cggggtgtta caacagttcg ctacgcctcg tgaactttat cacaaacccg agaatgtttt tgtcgcagga ttcatagggt ctcccggaat gaacttggtc accctctcga tcgtcgattc ctctgttttg ctgggggatt ggccgattcg cataccgcgt gaaatcgcca gcgccgcaag cgaggtcatc atcggcgtcc gtcccgaaca tttcgagctg ggcagcctcg gtgtcgaagt ggagatcgac atggtcgaag agctcggggc cgatgcttat ctatatggcc gaatcgcagg tgccagcaaa gtaaccgacc aacttgtcgt tgcacgcgtc gacggccgaa acccgccggc aaagggcagc cgcgttcgtc tgtatacaga acccgggaat gtgcatttct tcggcgttga tggccatcga atttctaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa