SSGCID: Target Page

MyleA.00750.a

Phosphate transport system ABC transporter

CENTER ID:	MyleA.00750.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00750.a.A1.GE29234	Full length( MyleA.00750.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.4168
RefSeq:	YP_002504064.1
UniProt:	A0A0H3MRX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKRLDLKGV NIYYGSFQAV LDVSLAVLPR SVTAFIGASG CGKTTVLRTL NRMHEVVPGA RVEGTVLLDD EDIYAPGIDP VGVRRAIGMV FQRPNPFPAM SIRDNVVAGL KLQGVRNRKV LDDTAEYFLR GTNLWDEVKD RLDKPGGGLS GGQQQRLCIA RAIAVQPDVL LMDEPCSSLD PISTMAIEEL IGELKQEYTI VIVTHNMQQA ARVSDQTAFF NLEAVGRPGR LVEIDDTEKI FSNPTEKATE DYISGRFG

NT Sequence

GTGGCCAAGA GATTGGATCT CAAAGGCGTC AACATTTATT ACGGATCTTT TCAAGCAGTT TTGGATGTGT CGCTGGCGGT ACTGCCCAGA AGCGTGACGG CGTTCATCGG AGCCTCAGGC TGCGGCAAGA CGACGGTGTT GCGCACCTTA AATCGGATGC ATGAGGTCGT CCCTGGCGCC CGTGTTGAGG GCACCGTGTT ACTCGACGAT GAAGACATTT ATGCCCCGGG TATCGATCCC GTCGGTGTGC GACGGGCGAT CGGGATGGTG TTCCAGCGGC CCAACCCGTT CCCCGCCATG TCAATTCGCG ATAACGTGGT GGCCGGTTTG AAGCTGCAAG GCGTGCGTAA TCGTAAGGTG CTTGATGATA CCGCAGAATA CTTTTTGCGC GGTACGAATC TGTGGGATGA GGTCAAGGAC CGATTGGATA AGCCTGGCGG TGGACTGTCC GGGGGGCAGC AGCAACGGCT GTGTATCGCT CGGGCCATTG CCGTGCAACC AGACGTGTTA CTCATGGACG AGCCGTGCTC CTCGCTCGAT CCCATTTCGA CGATGGCTAT CGAAGAACTG ATCGGTGAAT TGAAACAGGA GTACACCATT GTAATAGTTA CCCACAACAT GCAGCAGGCG GCGCGGGTGA GCGATCAGAC TGCGTTCTTC AACTTGGAAG CCGTAGGAAG ACCCGGCCGG CTGGTGGAGA TAGATGACAC CGAGAAAATC TTTTCCAATC CGACCGAAAA GGCCACTGAG GACTACATCT CCGGCCGCTT CGGC

Details for MyleA.00750.a.A1.GE29234

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMVAKRLDLK GVNIYYGSFQ AVLDVSLAVL PRSVTAFIGA SGCGKTTVLR TLNRMHEVVP GARVEGTVLL DDEDIYAPGI DPVGVRRAIG MVFQRPNPFP AMSIRDNVVA GLKLQGVRNR KVLDDTAEYF LRGTNLWDEV KDRLDKPGGG LSGGQQQRLC IARAIAVQPD VLLMDEPCSS LDPISTMAIE ELIGELKQEY TIVIVTHNMQ QAARVSDQTA FFNLEAVGRP GRLVEIDDTE KIFSNPTEKA TEDYISGRFG

Validated NT Sequence

ttattagccg aagcggccgg agatgtagtc ctcagtggcc ttttcggtcg gattggaaaa gattttctcg gtgtcatcta tctccaccag ccggccgggt cttcctacgg cttccaagtt gaagaacgca gtctgatcgc tcacccgcgc cgcctgctgc atgttgtggg taactattac aatggtgtac tcctgtttca attcaccgat cagttcttcg atagccatcg tcgaaatggg atcgagcgag gagcacggct cgtccatgag taacacgtct ggttgcacgg caatggcccg agcgatacac agccgttgct gctgcccccc ggacagtcca ccgccaggct tatccaatcg gtccttgacc tcatcccaca gattcgtacc gcgcaaaaag tattctgcgg tatcatcaag caccttacga ttacgcacgc cttgcagctt caaaccggcc accacgttat cgcgaattga catggcgggg aacgggttgg gccgctggaa caccatcccg atcgcccgtc gcacaccgac gggatcgata cccggggcat aaatgtcttc atcgtcgagt aacacggtgc cctcaacacg ggcgccaggg acgacctcat gcatccgatt taaggtgcgc aacaccgtcg tcttgccgca gcctgaggct ccgatgaacg ccgtcacgct tctgggcagt accgccagcg acacatccaa aactgcttga aaagatccgt aataaatgtt gacgcctttg agatccaatc tcttggccac catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatggt gatggtgatg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggccaag agattggatc tcaaaggcgt caacatttat tacggatctt ttcaagcagt tttggatgtg tcgctggcgg tactgcccag aagcgtgacg gcgttcatcg gagcctcagg ctgcggcaag acgacggtgt tgcgcacctt aaatcggatg catgaggtcg tccctggcgc ccgtgttgag ggcaccgtgt tactcgacga tgaagacatt tatgccccgg gtatcgatcc cgtcggtgtg cgacgggcga tcgggatggt gttccagcgg cccaacccgt tccccgccat gtcaattcgc gataacgtgg tggccggttt gaagctgcaa ggcgtgcgta atcgtaaggt gcttgatgat accgcagaat actttttgcg cggtacgaat ctgtgggatg aggtcaagga ccgattggat aagcctggcg gtggactgtc cggggggcag cagcaacggc tgtgtatcgc tcgggccatt gccgtgcaac cagacgtgtt actcatggac gagccgtgct cctcgctcga tcccatttcg acgatggcta tcgaagaact gatcggtgaa ttgaaacagg agtacaccat tgtaatagtt acccacaaca tgcagcaggc ggcgcgggtg agcgatcaga ctgcgttctt caacttggaa gccgtaggaa gacccggccg gctggtggag atagatgaca ccgagaaaat cttttccaat ccgaccgaaa aggccactga ggactacatc tccggccgct tcggcaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa