MyleA.00746.a

Probable ABC-transport protein, ATP-binding component

CENTER ID: MyleA.00746.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00746.a.A1.GE29233 Full length( MyleA.00746.a ) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.1951
RefSeq: YP_002503427.1
UniProt: A0A0H3MQC3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAEIVLEHVN KNYPNGATAV HDLSITVADG EFLILIGPSG CGKTTTLNMI AGLEDISSGE LRIDGDRVNE KAPKDRDIAM VFQSYALYPH MTVRQNIAFP LMLAKVKKAE IAQKVSETAQ ILDLTDLLDR KPSQLSGGQR QRVAMGRAIV RHPKAFLMDE PLSNLDAKLR VRTRGEIARL QRRLGATTVY VTHDQTEAMT LGDRVVVMRS GVVQQIGTPD ELYERPVNLF VAGFIGSPVM NFFPAKLTPN GLTLPLGELN LSRDVQAMIG QHPVPDRVIV GVRPEHLTDA KLIDAHQHGT VLTFKVKVDL VESLGADKYI YFTTTGCDVY SAQLDELASE LEVRENQFVA RVSAESKMAI GESIELAFGT AKIAVFDADS GVNLTVSAPT DA
NT Sequence
ATGGCCGAGA TTGTGCTGGA GCACGTCAAC AAGAATTACC CGAACGGTGC GACGGCGGTG CATGACCTTT CCATCACCGT CGCCGACGGG GAATTCCTCA TCCTGATAGG GCCTTCAGGC TGCGGCAAGA CCACGACTCT GAATATGATT GCTGGACTTG AGGATATTTC TTCGGGTGAG CTAAGAATCG ACGGCGATCG GGTCAACGAG AAGGCGCCCA AGGATCGTGA TATCGCGATG GTGTTCCAGT CTTACGCGCT TTACCCGCAT ATGACCGTGC GACAGAATAT CGCGTTCCCG CTGATGCTGG CCAAGGTGAA GAAAGCGGAG ATAGCGCAGA AGGTTTCCGA GACTGCTCAA ATCCTTGATT TGACCGATCT TTTGGACCGT AAGCCCTCGC AGTTGTCTGG CGGGCAACGA CAACGGGTCG CGATGGGCCG GGCAATTGTT CGTCACCCCA AGGCATTCTT GATGGACGAG CCGTTGTCCA ACCTGGATGC TAAACTACGG GTGCGGACGC GTGGTGAAAT CGCCCGGCTG CAGCGCAGGT TGGGTGCCAC CACCGTTTAT GTCACCCACG ATCAAACTGA AGCCATGACG CTAGGGGATC GCGTGGTGGT CATGCGCTCT GGTGTTGTAC AGCAGATCGG TACGCCCGAT GAGCTTTATG AACGTCCGGT CAATCTCTTC GTCGCGGGGT TTATCGGTTC GCCGGTGATG AATTTCTTCC CTGCCAAGTT GACACCGAAC GGACTGACCC TGCCCTTGGG GGAACTGAAC TTGTCGCGGG ACGTCCAGGC GATGATCGGA CAGCACCCGG TGCCGGACAG GGTTATTGTC GGGGTGCGAC CCGAGCATCT GACCGATGCT AAGTTGATCG ACGCACATCA ACACGGCACG GTGTTAACTT TTAAGGTGAA GGTTGACTTG GTCGAATCAC TGGGGGCCGA CAAATACATC TATTTTACTA CGACTGGTTG TGATGTGTAT TCAGCCCAGT TGGACGAGTT AGCTTCCGAG TTGGAAGTAC GTGAAAATCA GTTTGTGGCA AGAGTTTCCG CTGAATCAAA AATGGCCATA GGAGAGTCGA TTGAGTTGGC TTTCGGCACA GCAAAAATAG CTGTTTTCGA CGCTGACTCT GGGGTGAATC TGACTGTTTC TGCACCGACT GATGCG
Details for MyleA.00746.a.A1.GE29233
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 94
Validated AA Sequence
MAEIVLEHVN KNYPNGATAV HDLSITVADG EFLILIGPSG CGKTTTLNMI AGLEDISSGE LRIDGDRVNE KAPKDRDIAM VFQSYALYPH MTVRQNIAFP LMLAKXKKAE IAQKVSETAQ ILDLTDLLDR KPSQLSGGQR QRVAMGRAIV RHPKAFLMDE PLSNLDAKLR VRTRGEIARL QRRLGATTVY VTHDQTEAMT LGDRVVVMRS GVVQQIGTPD ELYERPVNLF VAGFIGSPVM NFFPAKLTPN GLTLPLGELN LSRDVQAMIG QHPVPDRVIV GVRPEHLTDA KLIDAHQHGT VLTFKVKVDL VESLGADKYI YFTTTGCDVY SAQLDELASE LEVRENQFVA RVSAESKMAI GESIELAFGT AKIAVFDADS GVNLTVSAPT
Validated NT Sequence
cagtcggtgc agaaacagtc agattcaccc cagagtcagc gtcgaaaaca gctatttttg ctgtgccgaa agccaactca atcgactctc ctatggccat ttttgattca gcggaaactc ttgccacaaa ctgattttca cgtacttcca actcggaagc taactcgtcc aactgggctg aatacacatc acaaccagtc gtagtaaaat agatgtattt gtcggccccc agtgattcga ccaagtcaac cttcacctta aaagttaaca ccgtgccgtg ttgatgtgcg tcgatcaact tagcatcggt cagatgctcg ggtcgcaccc cgacaataac cctgtccggc accgggtgct gtccgatcat cgcctggacg tcccgcgaca agttcagttc ccccaagggc agggtcagtc cgttcggtgt caacttggca gggaagaaat tcatcaccgg cgaaccgata aaccccgcga cgaagagatt gaccggacgt tcataaagct catcgggcgt accgatctgc tgtacaacac cagagcgcat gaccaccacg cgatccccta gcgtcatggc ttcagtttga tcgtgggtga cataaacggt ggtggcaccc aacctgcgct gcagccgggc gatttcacca cgcgtccgca cccgtagttt agcatccagg ttggacaacg gctcgtccat caagaatgcc ttggggtgac gaacaattgc ccggcccatc gcgacccgtt gtcgttgccc gccagacaac tgcgagggct tacggtccaa aagatcggtc aaatcaagga tttgagcagt ctcggaaacc ttctgcgcta tctccgcttt cttcnccttg gccagcatca gcgggaacgc gatattctgt cgcacggtca tatgcgggta aagcgcgtaa gactggaaca ccatcgcgat atcacgatcc ttgggcgcct tctcgttgac ccgatcgccg tcgattctta gctcacccga agaaatatcc tcaagtccag caatcatatt cagagtcgtg gtcttgccgc agcctgaagg ccctatcagg atgaggaatt ccccgtcggc gacggtgatg gaaaggtcat gcaccgccgt cgcaccgttc gggtaattct tgttgacgtg ctccagcaca atctcggcca tcgaaccagg accctgggtc tgagcttcca gggtaccc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAEIVLEHV NKNYPNGATA VHDLSITVAD GEFLILIGPS GCGKTTTLNM IAGLEDISSG ELRIDGDRVN EKAPKDRDIA MVFQSYALYP HMTVRQNIAF PLMLAKVKKA EIAQKVSETA QILDLTDLLD RKPSQLSGGQ RQRVAMGRAI VRHPKAFLMD EPLSNLDAKL RVRTRGEIAR LQRRLGATTV YVTHDQTEAM TLGDRVVVMR SGVVQQIGTP DELYERPVNL FVAGFIGSPV MNFFPAKLTP NGLTLPLGEL NLSRDVQAMI GQHPVPDRVI VGVRPEHLTD AKLIDAHQHG TVLTFKVKVD LVESLGADKY IYFTTTGCDV YSAQLDELAS ELEVRENQFV ARVSAESKMA IGESIELAFG TAKIAVFDAD SGVNLTVSAP TDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgagatt gtgctggagc acgtcaacaa gaattacccg aacggtgcga cggcggtgca tgacctttcc atcaccgtcg ccgacgggga attcctcatc ctgatagggc cttcaggctg cggcaagacc acgactctga atatgattgc tggacttgag gatatttctt cgggtgagct aagaatcgac ggcgatcggg tcaacgagaa ggcgcccaag gatcgtgata tcgcgatggt gttccagtct tacgcgcttt acccgcatat gaccgtgcga cagaatatcg cgttcccgct gatgctggcc aaggtgaaga aagcggagat agcgcagaag gtttccgaga ctgctcaaat ccttgatttg accgatcttt tggaccgtaa gccctcgcag ttgtctggcg ggcaacgaca acgggtcgcg atgggccggg caattgttcg tcaccccaag gcattcttga tggacgagcc gttgtccaac ctggatgcta aactacgggt gcggacgcgt ggtgaaatcg cccggctgca gcgcaggttg ggtgccacca ccgtttatgt cacccacgat caaactgaag ccatgacgct aggggatcgc gtggtggtca tgcgctctgg tgttgtacag cagatcggta cgcccgatga gctttatgaa cgtccggtca atctcttcgt cgcggggttt atcggttcgc cggtgatgaa tttcttccct gccaagttga caccgaacgg actgaccctg cccttggggg aactgaactt gtcgcgggac gtccaggcga tgatcggaca gcacccggtg ccggacaggg ttattgtcgg ggtgcgaccc gagcatctga ccgatgctaa gttgatcgac gcacatcaac acggcacggt gttaactttt aaggtgaagg ttgacttggt cgaatcactg ggggccgaca aatacatcta ttttactacg actggttgtg atgtgtattc agcccagttg gacgagttag cttccgagtt ggaagtacgt gaaaatcagt ttgtggcaag agtttccgct gaatcaaaaa tggccatagg agagtcgatt gagttggctt tcggcacagc aaaaatagct gttttcgacg ctgactctgg ggtgaatctg actgtttctg caccgactga tgcgaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa