MyleA.00713.a

PROBABLE GUANYLATE KINASE GMK

CENTER ID: MyleA.00713.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00713.a.A1.GE34170 full length 1 210
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00713.a.A1.PW37630 full length 1 210

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.937
RefSeq: YP_002503091.1
UniProt: A0A0H3MPX6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVSARGAPD AEHWAWSEQT DKGRVVVLSG PSAVGKSTVV RCLRERVSNL HFSVSATTRE PRPDEMDGVD YHFVSPARFQ QLIDQGALLE WAEIHGGMHR SGTLAEPVRV AAAAGFPVLI EVDLAGARAV KKAMPEAIAV FLAPPSWEDL EARLVGRGTE TPEAIRRRLE TARIELAAQD DFDEVVVNRR LESACAELVS LLVGAVSGSA
NT Sequence
gtgccagtga gcgcgcgcgg ggcaccggat gctgagcact gggcatggag tgagcaaacg gataaaggac gtgtggttgt gctgtccggt ccctcggcgg tcggcaagtc aacggtggtc aggtgtctgc gcgaacgggt gtcgaacctg catttcagtg tgtcggccac gacgcgagag cctaggccag atgagatgga cggtgtcgat tatcacttcg tcagccccgc ccgattccaa cagctgatcg accagggtgc attgctggaa tgggctgaaa tccacggcgg gatgcaccgt tcgggcaccc tggccgagcc ggtgcgggtc gccgctgcgg ccggttttcc ggtgcttatc gaggttgacc tggccggagc cagggcggtc aagaaggcga tgcctgaggc catcgccgtg tttttagcgc cgcccagctg ggaggatttg gaagccaggc tggttggccg cggcaccgaa acacccgagg ccattcggcg ccggctcgag accgcgcgca tcgaattggc ggcacaagat gacttcgatg aagttgtggt caaccgtcga ttggagtctg cgtgtgccga attggtatcc ttgctggtgg gagccgtgtc gggctcggca tga
Details for MyleA.00713.a.A1.GE34170
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 25kDa
OBSERVED MW: 26kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSARGA PDAEHWAWSE QTDKGRVVVL SGPSAVGKST VVRCLRERVS NLHFSVSATT REPRPDEMDG VDYHFVSPAR FQQLIDQGAL LEWAEIHGGM HRSGTLAEPV RVAAAAGFPV LIEVDLAGAR AVKKAMPEAI AVFLAPPSWE DLEARLVGRG TETPEAIRRR LETARIELAA QDDFDEVVVN RRLESACAEL VSLLVGAVSG SA
Validated NT Sequence
ttattatgcc gagcccgaca cggctcccac cagcaaggat accaattcgg cacacgcaga ctccaatcga cggttgacca caacttcatc gaagtcatct tgtgccgcca attcgatgcg cgcggtctcg agccggcgcc gaatggcctc gggtgtttcg gtgccgcggc caaccagcct ggcttccaaa tcctcccagc tgggcggcgc taaaaacacg gcgatggcct caggcatcgc cttcttgacc gccctggctc cggccaggtc aacctcgata agcaccggaa aaccggccgc agcggcgacc cgcaccggct cggccagggt gcccgaacgg tgcatcccgc cgtggatttc agcccattcc agcaatgcac cctggtcgat cagctgttgg aatcgggcgg ggctgacgaa gtgataatcg acaccgtcca tctcatctgg cctaggctct cgcgtcgtgg ccgacacact gaaatgcagg ttcgacaccc gttcgcgcag acacctgacc accgttgact tgccgaccgc cgagggaccg gacagcacaa ccacacgtcc tttatccgtt tgctcactcc atgcccagtg ctcagcatcc ggtgccccgc gcgcgctcac tggcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSARGA PDAEHWAWSE QTDKGRVVVL SGPSAVGKST VVRCLRERVS NLHFSVSATT REPRPDEMDG VDYHFVSPAR FQQLIDQGAL LEWAEIHGGM HRSGTLAEPV RVAAAAGFPV LIEVDLAGAR AVKKAMPEAI AVFLAPPSWE DLEARLVGRG TETPEAIRRR LETARIELAA QDDFDEVVVN RRLESACAEL VSLLVGAVSG SA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgccagtg agcgcgcgcg gggcaccgga tgctgagcac tgggcatgga gtgagcaaac ggataaagga cgtgtggttg tgctgtccgg tccctcggcg gtcggcaagt caacggtggt caggtgtctg cgcgaacggg tgtcgaacct gcatttcagt gtgtcggcca cgacgcgaga gcctaggcca gatgagatgg acggtgtcga ttatcacttc gtcagccccg cccgattcca acagctgatc gaccagggtg cattgctgga atgggctgaa atccacggcg ggatgcaccg ttcgggcacc ctggccgagc cggtgcgggt cgccgctgcg gccggttttc cggtgcttat cgaggttgac ctggccggag ccagggcggt caagaaggcg atgcctgagg ccatcgccgt gtttttagcg ccgcccagct gggaggattt ggaagccagg ctggttggcc gcggcaccga aacacccgag gccattcggc gccggctcga gaccgcgcgc atcgaattgg cggcacaaga tgacttcgat gaagttgtgg tcaaccgtcg attggagtct gcgtgtgccg aattggtatc cttgctggtg ggagccgtgt cgggctcggc ataaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for MyleA.00713.a.A1.PW37630
PURIFICATION DATe: 1/13/2015
CONCENTRATION: 9mg/ml
OBSERVED MW: 26kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSARGA PDAEHWAWSE QTDKGRVVVL SGPSAVGKST VVRCLRERVS NLHFSVSATT REPRPDEMDG VDYHFVSPAR FQQLIDQGAL LEWAEIHGGM HRSGTLAEPV RVAAAAGFPV LIEVDLAGAR AVKKAMPEAI AVFLAPPSWE DLEARLVGRG TETPEAIRRR LETARIELAA QDDFDEVVVN RRLESACAEL VSLLVGAVSG SA
Validated NT Sequence
ttattatgcc gagcccgaca cggctcccac cagcaaggat accaattcgg cacacgcaga ctccaatcga cggttgacca caacttcatc gaagtcatct tgtgccgcca attcgatgcg cgcggtctcg agccggcgcc gaatggcctc gggtgtttcg gtgccgcggc caaccagcct ggcttccaaa tcctcccagc tgggcggcgc taaaaacacg gcgatggcct caggcatcgc cttcttgacc gccctggctc cggccaggtc aacctcgata agcaccggaa aaccggccgc agcggcgacc cgcaccggct cggccagggt gcccgaacgg tgcatcccgc cgtggatttc agcccattcc agcaatgcac cctggtcgat cagctgttgg aatcgggcgg ggctgacgaa gtgataatcg acaccgtcca tctcatctgg cctaggctct cgcgtcgtgg ccgacacact gaaatgcagg ttcgacaccc gttcgcgcag acacctgacc accgttgact tgccgaccgc cgagggaccg gacagcacaa ccacacgtcc tttatccgtt tgctcactcc atgcccagtg ctcagcatcc ggtgccccgc gcgcgctcac tggcaccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgagcc at
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVPVSARGA PDAEHWAWSE QTDKGRVVVL SGPSAVGKST VVRCLRERVS NLHFSVSATT REPRPDEMDG VDYHFVSPAR FQQLIDQGAL LEWAEIHGGM HRSGTLAEPV RVAAAAGFPV LIEVDLAGAR AVKKAMPEAI AVFLAPPSWE DLEARLVGRG TETPEAIRRR LETARIELAA QDDFDEVVVN RRLESACAEL VSLLVGAVSG SA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGCCAGTG AGCGCGCGCG GGGCACCGGA TGCTGAGCAC TGGGCATGGA GTGAGCAAAC GGATAAAGGA CGTGTGGTTG TGCTGTCCGG TCCCTCGGCG GTCGGCAAGT CAACGGTGGT CAGGTGTCTG CGCGAACGGG TGTCGAACCT GCATTTCAGT GTGTCGGCCA CGACGCGAGA GCCTAGGCCA GATGAGATGG ACGGTGTCGA TTATCACTTC GTCAGCCCCG CCCGATTCCA ACAGCTGATC GACCAGGGTG CATTGCTGGA ATGGGCTGAA ATCCACGGCG GGATGCACCG TTCGGGCACC CTGGCCGAGC CGGTGCGGGT CGCCGCTGCG GCCGGTTTTC CGGTGCTTAT CGAGGTTGAC CTGGCCGGAG CCAGGGCGGT CAAGAAGGCG ATGCCTGAGG CCATCGCCGT GTTTTTAGCG CCGCCCAGCT GGGAGGATTT GGAAGCCAGG CTGGTTGGCC GCGGCACCGA AACACCCGAG GCCATTCGGC GCCGGCTCGA GACCGCGCGC ATCGAATTGG CGGCACAAGA TGACTTCGAT GAAGTTGTGG TCAACCGTCG ATTGGAGTCT GCGTGTGCCG AATTGGTATC CTTGCTGGTG GGAGCCGTGT CGGGCTCGGC ATAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA