MyleA.00681.b

SECRETED L-ALANINE DEHYDROGENASE ALD (40 KDA ANTIGEN) (TB43)

CENTER ID: MyleA.00681.b
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00681.b.A1.GE34169 full length 1 371
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.2904
RefSeq: YP_002503698.1
UniProt: A0A0H3MQZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRVGIPTETK NNEFRVAITP AGVAALTHRG HEVLVQAGAG EGSAIADAEF KAAGAQLIST PEQVWTEVDL LLKVKEPMPA EYVHLRHGQI LFTYLHLAAS RPCTDALLAS GTTSIAYETV QTADGALPLL APMSEVAGRL SAQVGAYHLM RTHRGCGVLM GGVPGVEPAD VVVIGGGTAG YNAARIANGM GATVTVFDVN INRLRELDAE FGGRLRTRYS SAYDLEGAVK HADLVIGAVL VPGAKAPRLI SNSLVAHMKP GAVLVDIAID QGGCFEDSQP TTHDQPTFAV HDTLFYCVAN MPSAVPKTST FALTNATMPY ILKLADQGWQ EACQSDPALA KGLSTHNGAL LSEQVATDLG LAFTEPASVL A
NT Sequence
atgcgagtcg gcattccgac cgagaccaaa aacaacgaat tcagggtggc catcacgccg gccggcgtgg ccgccctgac acaccgcggc cacgaggtgc ttgtgcaagc aggcgccgga gaaggctcgg caatcgccga cgcggagttc aaggcagcag gagcccaact gatcagcact cccgagcagg tgtggaccga ggtcgacctg ctactcaagg tcaaagagcc catgcccgca gagtatgttc acctacgaca cggccagatc ctgtttacgt atctgcatct agctgcctca cgcccttgca ccgacgcatt attggcctcc ggcacaacgt caatcgccta cgagaccgtg cagaccgccg acggcgcgct gccgttacta gctccaatga gcgaggttgc cggacgactg tcggctcagg taggggccta tcacctaatg cggacccaca gggggtgcgg ggtactgatg ggtggagtgc ccggcgtcga acccgctgac gtggtcgtga tcggcggcgg gactgcggga tataacgccg ctcgaattgc caacggtatg ggtgcgaccg tcacggtttt tgatgtcaac atcaacagac ttcgggagct cgacgcggag ttcggtggcc gcctccgcac ccgctactcg tcggcctacg acctagaagg tgcggtcaag cacgccgacc tggtgatcgg cgccgtcctg gtgccaggcg ccaaagcccc ccgattgata tcgaattcgc ttgtcgcaca tatgaagccg ggtgctgtgt tggtcgacat cgccatcgat cagggcggtt gtttcgaaga ttcgcagccg accacccatg atcaaccgac gttcgcggtg catgacacgc tgttctactg tgtggcaaac atgcctagcg cggtgcccaa gacatcgaca ttcgcgctga ccaatgcaac gatgccatat atactcaaac tcgcggacca aggctggcag gaagcctgcc aatcggatcc cgctttggca aaaggtcttt cgacgcataa cggtgcactg ttgtctgaac aggtagccac cgacctggga ttggccttca ccgaaccggc aagcgtgctg gcctga
Details for MyleA.00681.b.A1.GE34169
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/8/2011
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 81
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRVGIPTET KNNEFRVAIT PAGVAALTHR GHEVLVQAGA GEGSAIADAE FKAAGAQLIS TPEQVWTEVD LLLKVKEPMP AEYVHLRHGQ ILFTYLHLAA SRPCTDALLA SGTTSIAYET VQTADGALPL LAPMSEVAGR LSAQVGAYHL MRTHRGCGVL MGGVPGVEPA DVVVIGGGTA GYNAARIANG MGATVTVFDV NINRLRELDA EFGGRLRTRY SSAYDLXGAV KHADLVIGAV LVPGAKAPRL ISNSLVAHMK PGAVLVDXAX DQGGCFEXSQ PTTHDQPTFA XHDTLXYXXX XCXAXXXXSX FXLXXXXXXY SXSXXXXXXP XXXXXXXXXX XXLXXXTXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttttnnnnnn tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgcgagt cggcattccg accgagacca aaaacaacga attcagggtg gccatcacgc cggccggcgt ggccgccctg acacaccgcg gccacgaggt gcttgtgcaa gcaggcgccg gagaaggctc ggcaatcgcc gacgcggagt tcaaggcagc aggagcccaa ctgatcagca ctcccgagca ggtgtggacc gaggtcgacc tgctactcaa ggtcaaagag cccatgcccg cagagtatgt tcacctacga cacggccaga tcctgtttac gtatctgcat ctagctgcct cacgcccttg caccgacgca ttattggcct ccggcacaac gtcaatcgcc tacgagaccg tgcagaccgc cgacggcgcg ctgccgttac tagctccaat gagcgaggtt gccggacgac tgtcggctca ggtaggggcc tatcacctaa tgcggaccca cagggggtgc ggggtactga tgggtggagt gcccggcgtc gaacccgctg acgtggtcgt gatcggcggc gggactgcgg gatataacgc cgctcgaatt gccaacggta tgggtgcgac cgtcacggtt tttgatgtca acatcaacag acttcgggag ctcgacgcgg agttcggtgg ccgcctccgc acccgctact cgtcggccta cgacctanaa ggtgcggtca agcacgccga cctggtgatc ggcgccgtcc tggtgccagg cgccaaagcc ccccgattga tatcgaattc gcttgtcgca catatgaagc cgggtgctgt gttggtcgac ntcgccntcg atcagggcgg ttgtttcgaa gantcgcagc cnaccaccca tgatcaaccg acgttcgcgn tgcatgacac gctgtnctac tgngngnnaa natgcntagc gngngncnan anntcgannt tcnnnctgan nngnannatn cnnatntact cnanctcnnn nnnnnngnng nncgnnccnn nttgnnannn ntnnannnnn nncnnnnnnn nntgnctgan nnnnnnnact nnnnnnnntt nnncnnnnnn nnnnnnnnnn nnnnnnannn nnannnnnnn nnnnnnnnnn nnnnnnnnna a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRVGIPTET KNNEFRVAIT PAGVAALTHR GHEVLVQAGA GEGSAIADAE FKAAGAQLIS TPEQVWTEVD LLLKVKEPMP AEYVHLRHGQ ILFTYLHLAA SRPCTDALLA SGTTSIAYET VQTADGALPL LAPMSEVAGR LSAQVGAYHL MRTHRGCGVL MGGVPGVEPA DVVVIGGGTA GYNAARIANG MGATVTVFDV NINRLRELDA EFGGRLRTRY SSAYDLEGAV KHADLVIGAV LVPGAKAPRL ISNSLVAHMK PGAVLVDIAI DQGGCFEDSQ PTTHDQPTFA VHDTLFYCVA NMPSAVPKTS TFALTNATMP YILKLADQGW QEACQSDPAL AKGLSTHNGA LLSEQVATDL GLAFTEPASV LA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgagtcggc attccgaccg agaccaaaaa caacgaattc agggtggcca tcacgccggc cggcgtggcc gccctgacac accgcggcca cgaggtgctt gtgcaagcag gcgccggaga aggctcggca atcgccgacg cggagttcaa ggcagcagga gcccaactga tcagcactcc cgagcaggtg tggaccgagg tcgacctgct actcaaggtc aaagagccca tgcccgcaga gtatgttcac ctacgacacg gccagatcct gtttacgtat ctgcatctag ctgcctcacg cccttgcacc gacgcattat tggcctccgg cacaacgtca atcgcctacg agaccgtgca gaccgccgac ggcgcgctgc cgttactagc tccaatgagc gaggttgccg gacgactgtc ggctcaggta ggggcctatc acctaatgcg gacccacagg gggtgcgggg tactgatggg tggagtgccc ggcgtcgaac ccgctgacgt ggtcgtgatc ggcggcggga ctgcgggata taacgccgct cgaattgcca acggtatggg tgcgaccgtc acggtttttg atgtcaacat caacagactt cgggagctcg acgcggagtt cggtggccgc ctccgcaccc gctactcgtc ggcctacgac ctagaaggtg cggtcaagca cgccgacctg gtgatcggcg ccgtcctggt gccaggcgcc aaagcccccc gattgatatc gaattcgctt gtcgcacata tgaagccggg tgctgtgttg gtcgacatcg ccatcgatca gggcggttgt ttcgaagatt cgcagccgac cacccatgat caaccgacgt tcgcggtgca tgacacgctg ttctactgtg tggcaaacat gcctagcgcg gtgcccaaga catcgacatt cgcgctgacc aatgcaacga tgccatatat actcaaactc gcggaccaag gctggcagga agcctgccaa tcggatcccg ctttggcaaa aggtctttcg acgcataacg gtgcactgtt gtctgaacag gtagccaccg acctgggatt ggccttcacc gaaccggcaa gcgtgctggc ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa