MyleA.00681.a

Pyridine transhydrogenase subunit [alpha]1

CENTER ID: MyleA.00681.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00681.a.A1.GE29229 Full length( MyleA.00681.a ) 1 367
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00681.a.A1.PS00721 Full length( MyleA.00681.a ) 1 367

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.5083
RefSeq: YP_002504318.1
UniProt: A0A0H3MSL4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTEAQTTVGV VAESGSDERR VALVPKAVAS LVNSGLAVVV ESGAGEGALL PEQLYTEAGA RIGDAWAADI VVKVAPPAAA EVGRLRRGQT LIGFLASRNA DNWIGELKRA GVQAFALEAI PRISRAQVMD ALSSQANVTG YKAVLLAASE STRFFPMLTT AAGTVKPAIV LVLGVGVAGL QALATAKRLG ARTTGYDVRP EVAEQVRSVG AQWLDVGIEA SGAGGYAREL TADERAQQQK ALEKAISGFD VVITTALVPG KPAPRLVTAA AVEAMKPGSV VVDLAGETGG NCELTEPGQT VIKHDVTIAA PLNLPATMPE HASELYSKNI KALLDLLIKD GKFAPDFDDE VVAESCVTRD REDSIDV
NT Sequence
ATGACAGAAG CGCAAACGAC GGTTGGAGTG GTCGCCGAGT CCGGTTCCGA CGAGCGGCGC GTCGCTTTGG TGCCAAAGGC AGTAGCGTCT CTAGTGAACA GCGGTCTAGC GGTCGTGGTC GAGTCCGGGG CGGGCGAAGG TGCATTGCTT CCCGAGCAGC TCTACACAGA GGCCGGCGCT CGCATCGGGG ACGCGTGGGC TGCCGACATC GTCGTCAAAG TGGCGCCGCC GGCTGCAGCC GAGGTCGGCC GACTGCGCCG CGGGCAGACG CTGATCGGCT TCCTTGCGTC CCGCAATGCC GACAACTGGA TCGGCGAGTT GAAACGAGCT GGTGTGCAAG CGTTCGCGCT CGAGGCAATC CCGCGCATCT CGCGTGCACA AGTGATGGAT GCGCTGTCGT CGCAGGCTAA CGTGACCGGG TATAAAGCAG TGTTGCTTGC GGCTTCTGAG TCTACCCGAT TTTTCCCGAT GCTGACGACA GCTGCAGGAA CGGTGAAGCC GGCCATCGTG CTGGTGCTCG GCGTCGGGGT GGCAGGTTTG CAAGCGCTGG CGACGGCCAA GCGCCTTGGC GCGCGCACCA CCGGCTACGA CGTGCGTCCT GAGGTGGCCG AGCAAGTCCG GTCGGTGGGT GCCCAGTGGC TTGACGTAGG AATCGAGGCT TCCGGTGCGG GCGGATACGC CCGTGAGCTC ACCGCCGACG AACGCGCTCA GCAACAGAAG GCATTGGAAA AGGCGATCAG CGGATTCGAC GTGGTGATTA CCACAGCGCT GGTCCCGGGC AAGCCGGCAC CGCGGTTGGT GACCGCTGCG GCGGTGGAGG CGATGAAGCC TGGCAGCGTG GTGGTGGACC TCGCGGGGGA AACCGGTGGC AATTGCGAGC TCACTGAACC CGGGCAGACG GTGATCAAGC ATGATGTCAC TATTGCGGCG CCGCTGAATC TGCCGGCAAC GATGCCTGAG CACGCCAGCG AGCTCTACAG CAAGAACATC AAGGCGCTGT TGGACCTACT AATAAAGGAT GGCAAGTTTG CCCCGGACTT TGACGACGAA GTCGTCGCGG AGTCATGTGT GACTCGCGAT CGAGAGGACT CCATAGATGT A
Details for MyleA.00681.a.A1.GE29229
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MTEAQTTVGV VAESGSDERR VALVPKAVAS LVNSGLAVVV ESGAGEGALL PEQLYTEAGA RIGDAWAADI VVKVAPPAAA EVGRLRRGQT LIGFLASRNA DNWIGELKRA GVQAFALEAI PRISRAQVMD ALSSQANVTG YKAVLLAASE STRFFPMLTT AAGTVKPAIV LVLGVGVAGL QALATAKRLG ARTTGYDVRP EVAEQVRSVG AQWLDVGIEA SGAGGYAREL TADERAQQQK ALEKAISGFD VVITTALVPG KPAPRLVTAA AVEAMKPGSV VVDLAGETGG NCELTEPGQT VIKHDVTIAA PLNLPATMPE HASELYSKNI KALLDLLIKD GKFAPDFDDE VVAESCVTRD REDSI
Validated NT Sequence
ctatggagtc ctctcgatcg cgagtcacac atgactccgc gacgacttcg tcgtcaaagt ccggggcaaa cttgccatcc tttattagta ggtccaacag cgccttgatg ttcttgctgt agagctcgct ggcgtgctca ggcatcgttg ccggcagatt cagcggcgcc gcaatagtga catcatgctt gatcaccgtc tgcccgggtt cagtgagctc gcaattgcca ccggtttccc ccgcgaggtc caccaccacg ctgccaggct tcatcgcctc caccgccgca gcggtcacca accgcggtgc cggcttgccc gggaccagcg ctgtggtaat caccacgtcg aatccgctga tcgccttttc caatgccttc tgttgctgag cgcgttcgtc ggcggtgagc tcacgggcgt atccgcccgc accggaagcc tcgattccta cgtcaagcca ctgggcaccc accgaccgga cttgctcggc cacctcagga cgcacgtcgt agccggtggt gcgcgcgcca aggcgcttgg ccgtcgccag cgcttgcaaa cctgccaccc cgacgccgag caccagcacg atggccggct tcaccgttcc tgcagctgtc gtcagcatcg ggaaaaatcg ggtagactca gaagccgcaa gcaacactgc tttatacccg gtcacgttag cctgcgacga cagcgcatcc atcacttgtg cacgcgagat gcgcgggatt gcctcgagcg cgaacgcttg cacaccagct cgtttcaact cgccgatcca gttgtcggca ttgcgggacg caaggaagcc gatcagcgtc tgcccgcggc gcagtcggcc gacctcggct gcagccggcg gcgccacttt gacgacgatg tcggcagccc acgcgtcccc gatgcgagcg ccggcctctg tgtagagctg ctcgggaagc aatgcacctt cgcccgcccc ggactcgacc acgaccgcta gaccgctgtt cactagagac gctactgcct ttggcaccaa agcgacgcgc cgctcgtcgg aaccggactc ggcgaccact ccaaccgtcg tttgcgcttc tgtcatcgaa ccaggaccct gggtctgagc ttccanng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEAQTTVG VVAESGSDER RVALVPKAVA SLVNSGLAVV VESGAGEGAL LPEQLYTEAG ARIGDAWAAD IVVKVAPPAA AEVGRLRRGQ TLIGFLASRN ADNWIGELKR AGVQAFALEA IPRISRAQVM DALSSQANVT GYKAVLLAAS ESTRFFPMLT TAAGTVKPAI VLVLGVGVAG LQALATAKRL GARTTGYDVR PEVAEQVRSV GAQWLDVGIE ASGAGGYARE LTADERAQQQ KALEKAISGF DVVITTALVP GKPAPRLVTA AAVEAMKPGS VVVDLAGETG GNCELTEPGQ TVIKHDVTIA APLNLPATMP EHASELYSKN IKALLDLLIK DGKFAPDFDD EVVAESCVTR DREDSIDV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacagaagcg caaacgacgg ttggagtggt cgccgagtcc ggttccgacg agcggcgcgt cgctttggtg ccaaaggcag tagcgtctct agtgaacagc ggtctagcgg tcgtggtcga gtccggggcg ggcgaaggtg cattgcttcc cgagcagctc tacacagagg ccggcgctcg catcggggac gcgtgggctg ccgacatcgt cgtcaaagtg gcgccgccgg ctgcagccga ggtcggccga ctgcgccgcg ggcagacgct gatcggcttc cttgcgtccc gcaatgccga caactggatc ggcgagttga aacgagctgg tgtgcaagcg ttcgcgctcg aggcaatccc gcgcatctcg cgtgcacaag tgatggatgc gctgtcgtcg caggctaacg tgaccgggta taaagcagtg ttgcttgcgg cttctgagtc tacccgattt ttcccgatgc tgacgacagc tgcaggaacg gtgaagccgg ccatcgtgct ggtgctcggc gtcggggtgg caggtttgca agcgctggcg acggccaagc gccttggcgc gcgcaccacc ggctacgacg tgcgtcctga ggtggccgag caagtccggt cggtgggtgc ccagtggctt gacgtaggaa tcgaggcttc cggtgcgggc ggatacgccc gtgagctcac cgccgacgaa cgcgctcagc aacagaaggc attggaaaag gcgatcagcg gattcgacgt ggtgattacc acagcgctgg tcccgggcaa gccggcaccg cggttggtga ccgctgcggc ggtggaggcg atgaagcctg gcagcgtggt ggtggacctc gcgggggaaa ccggtggcaa ttgcgagctc actgaacccg ggcagacggt gatcaagcat gatgtcacta ttgcggcgcc gctgaatctg ccggcaacga tgcctgagca cgccagcgag ctctacagca agaacatcaa ggcgctgttg gacctactaa taaaggatgg caagtttgcc ccggactttg acgacgaagt cgtcgcggag tcatgtgtga ctcgcgatcg agaggactcc atagatgtaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyleA.00681.a.A1.PS00721
PURIFICATION DATe: 8/12/2010
CONCENTRATION: 7.8mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MTEAQTTVGV VAESGSDERR VALVPKAVAS LVNSGLAVVV ESGAGEGALL PEQLYTEAGA RIGDAWAADI VVKVAPPAAA EVGRLRRGQT LIGFLASRNA DNWIGELKRA GVQAFALEAI PRISRAQVMD ALSSQANVTG YKAVLLAASE STRFFPMLTT AAGTVKPAIV LVLGVGVAGL QALATAKRLG ARTTGYDVRP EVAEQVRSVG AQWLDVGIEA SGAGGYAREL TADERAQQQK ALEKAISGFD VVITTALVPG KPAPRLVTAA AVEAMKPGSV VVDLAGETGG NCELTEPGQT VIKHDVTIAA PLNLPATMPE HASELYSKNI KALLDLLIKD GKFAPDFDDE VVAESCVTRD REDSI
Validated NT Sequence
ctatggagtc ctctcgatcg cgagtcacac atgactccgc gacgacttcg tcgtcaaagt ccggggcaaa cttgccatcc tttattagta ggtccaacag cgccttgatg ttcttgctgt agagctcgct ggcgtgctca ggcatcgttg ccggcagatt cagcggcgcc gcaatagtga catcatgctt gatcaccgtc tgcccgggtt cagtgagctc gcaattgcca ccggtttccc ccgcgaggtc caccaccacg ctgccaggct tcatcgcctc caccgccgca gcggtcacca accgcggtgc cggcttgccc gggaccagcg ctgtggtaat caccacgtcg aatccgctga tcgccttttc caatgccttc tgttgctgag cgcgttcgtc ggcggtgagc tcacgggcgt atccgcccgc accggaagcc tcgattccta cgtcaagcca ctgggcaccc accgaccgga cttgctcggc cacctcagga cgcacgtcgt agccggtggt gcgcgcgcca aggcgcttgg ccgtcgccag cgcttgcaaa cctgccaccc cgacgccgag caccagcacg atggccggct tcaccgttcc tgcagctgtc gtcagcatcg ggaaaaatcg ggtagactca gaagccgcaa gcaacactgc tttatacccg gtcacgttag cctgcgacga cagcgcatcc atcacttgtg cacgcgagat gcgcgggatt gcctcgagcg cgaacgcttg cacaccagct cgtttcaact cgccgatcca gttgtcggca ttgcgggacg caaggaagcc gatcagcgtc tgcccgcggc gcagtcggcc gacctcggct gcagccggcg gcgccacttt gacgacgatg tcggcagccc acgcgtcccc gatgcgagcg ccggcctctg tgtagagctg ctcgggaagc aatgcacctt cgcccgcccc ggactcgacc acgaccgcta gaccgctgtt cactagagac gctactgcct ttggcaccaa agcgacgcgc cgctcgtcgg aaccggactc ggcgaccact ccaaccgtcg tttgcgcttc tgtcatcgaa ccaggaccct gggtctgagc ttccanng
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTEAQTTVG VVAESGSDER RVALVPKAVA SLVNSGLAVV VESGAGEGAL LPEQLYTEAG ARIGDAWAAD IVVKVAPPAA AEVGRLRRGQ TLIGFLASRN ADNWIGELKR AGVQAFALEA IPRISRAQVM DALSSQANVT GYKAVLLAAS ESTRFFPMLT TAAGTVKPAI VLVLGVGVAG LQALATAKRL GARTTGYDVR PEVAEQVRSV GAQWLDVGIE ASGAGGYARE LTADERAQQQ KALEKAISGF DVVITTALVP GKPAPRLVTA AAVEAMKPGS VVVDLAGETG GNCELTEPGQ TVIKHDVTIA APLNLPATMP EHASELYSKN IKALLDLLIK DGKFAPDFDD EVVAESCVTR DREDSIDV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACAGAAGCG CAAACGACGG TTGGAGTGGT CGCCGAGTCC GGTTCCGACG AGCGGCGCGT CGCTTTGGTG CCAAAGGCAG TAGCGTCTCT AGTGAACAGC GGTCTAGCGG TCGTGGTCGA GTCCGGGGCG GGCGAAGGTG CATTGCTTCC CGAGCAGCTC TACACAGAGG CCGGCGCTCG CATCGGGGAC GCGTGGGCTG CCGACATCGT CGTCAAAGTG GCGCCGCCGG CTGCAGCCGA GGTCGGCCGA CTGCGCCGCG GGCAGACGCT GATCGGCTTC CTTGCGTCCC GCAATGCCGA CAACTGGATC GGCGAGTTGA AACGAGCTGG TGTGCAAGCG TTCGCGCTCG AGGCAATCCC GCGCATCTCG CGTGCACAAG TGATGGATGC GCTGTCGTCG CAGGCTAACG TGACCGGGTA TAAAGCAGTG TTGCTTGCGG CTTCTGAGTC TACCCGATTT TTCCCGATGC TGACGACAGC TGCAGGAACG GTGAAGCCGG CCATCGTGCT GGTGCTCGGC GTCGGGGTGG CAGGTTTGCA AGCGCTGGCG ACGGCCAAGC GCCTTGGCGC GCGCACCACC GGCTACGACG TGCGTCCTGA GGTGGCCGAG CAAGTCCGGT CGGTGGGTGC CCAGTGGCTT GACGTAGGAA TCGAGGCTTC CGGTGCGGGC GGATACGCCC GTGAGCTCAC CGCCGACGAA CGCGCTCAGC AACAGAAGGC ATTGGAAAAG GCGATCAGCG GATTCGACGT GGTGATTACC ACAGCGCTGG TCCCGGGCAA GCCGGCACCG CGGTTGGTGA CCGCTGCGGC GGTGGAGGCG ATGAAGCCTG GCAGCGTGGT GGTGGACCTC GCGGGGGAAA CCGGTGGCAA TTGCGAGCTC ACTGAACCCG GGCAGACGGT GATCAAGCAT GATGTCACTA TTGCGGCGCC GCTGAATCTG CCGGCAACGA TGCCTGAGCA CGCCAGCGAG CTCTACAGCA AGAACATCAA GGCGCTGTTG GACCTACTAA TAAAGGATGG CAAGTTTGCC CCGGACTTTG ACGACGAAGT CGTCGCGGAG TCATGTGTGA CTCGCGATCG AGAGGACTCC ATAGATGTAA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A