MyleA.00629.c

Acyl-CoA synthase

CENTER ID: MyleA.00629.c
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00629.c.B1.GE40021 full length 1 548
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.5141
RefSeq: WP_010909016.1
UniProt: A0A0H3MSI5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSESITSSR APVEQIDARL TGQFESPSIA SLVEAAAIRN PSAPALVVTD NRIVVSYRDL LRLVDDLTVQ LALGGLLPGD RVALCAASNI EFVVGLLAAS RAGLIVVPLD PALPVNEQCI RSQAAGVRVT LVDSLALEGV SDQRAATMRY WPIAVSYGSV TGASEGSLLV HLDDTAALHP VTSTPDGLRH DDAMIMFTGG TTGLPKMVPW TDGNIAGSVH AIITAYQLGP QDATVVVMPL YHGHGLIAAL LSTLASGGVV LLPARARFSA RTFWDDIDAV AATWYTAAPA IHRILLELAS TQSFRSKRAK LRFIRSCSAP LTQETAQALR EEFLAPVICA FGMTEATHQV TTTNIKWFGQ GENPTVTNGL VGQSTGVQIR IVGSDGQPLP PDTVGEVWLR GSTVVRGYLG DPAITAANFT HGWLRTGDLG SLSVTGDLRI RGRIKELINR SGEKISPERV EGVLASHHNV MEVAVFGDPD KVYGETVTAV IVPREVIAPT PSELAVFCRD RLAAFEVPTR FQEASALPHT AKGSLDRRAV AEQFAHRG
NT Sequence
atgaaatcgg agtcgattac cagcagcaga gctccggttg aacagataga tgcgaggctg actggccaat tcgaaagccc gagtatcgcc agcttggtcg aggcggcagc aattcggaat ccaagcgcgc cagcgcttgt cgtgaccgac aatcgcatag tggtgagcta ccgagatttg ctccgactgg tcgatgattt gaccgtccag ctggcgctgg gtggcctgct gcctggcgat cgggtagcgc tgtgcgcggc tagcaacatt gaattcgttg taggcttact agcggcgtcg cgagccggcc tgattgtggt gccgttagat ccggctctac ctgtcaatga gcaatgcatc cggagccaag cagcgggcgt gcgagtgaca ttggtggaca gtcttgccct tgaaggcgtt agcgaccagc gagcagcgac gatgcgctac tggccgattg cggtttccta cggtagcgtc acaggcgctt cggaaggcag cttgttggtc cacctggacg acactgcggc gttgcatcca gtcacttcga cgcctgacgg gctccggcac gatgacgcta tgatcatgtt cactggagga acgacgggtt tgccgaagat ggtcccgtgg acagacggga atatcgccgg ttccgttcac gccatcatta ccgcgtacca gctcggccca caggatgcga ctgtcgtggt gatgcccctc taccatggtc atgggctcat tgctgcgttg ctgtcgaccc tggcatctgg aggtgtggtg ttgctccctg ctcgcgcgcg gttctcggcg cgcacattct gggacgacat cgatgccgtt gcggctactt ggtacacggc cgcgccggcg attcatcgga tcttgttgga actcgcttct actcaatctt tccggagtaa gcgagccaaa ctccgcttta tccgcagttg cagtgcgccc cttacccagg aaacggcgca agcgctgcgt gaagagttct tggcgccggt gatatgtgct ttcggcatga ccgaggccac tcaccaggtg acaaccacaa atataaaatg gttcggccaa ggggagaatc ccaccgtaac aaacggtctt gtcggtcagt caactggcgt acagatccgc atcgtcgggt ccgacggtca acccctcccc ccggataccg taggggaggt ttggttgcgt ggctccacag tggtgcgtgg gtatctgggt gatccggcga taaccgcagc gaacttcacc cacggatggt tgcgtaccgg tgatctgggg tcactgtcgg taaccggtga cctgaggatc cgtggccgga tcaaggaact cataaaccgc tccggcgaga agatctcgcc ggagcgcgtc gaaggtgtat tggccagtca ccacaacgtt atggaggtag cggtatttgg tgatcctgac aaggtgtacg gggagacagt gaccgcagtg attgttcctc gcgaggtcat agcaccgact ccgtcggagc ttgcggtgtt ctgccgtgat cggttggcgg ccttcgaagt gcccaccaga tttcaggagg cgagcgcgct tccgcacacc gccaaaggtt cgctcgaccg ccgcgccgtg gccgaacagt tcgctcatcg ggggtga
Details for MyleA.00629.c.B1.GE40021
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 59kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMK SESITSSRAP VEQIDARLTG QFESPSIASL VEAAAIRNPS APALVVTDNR IVVSYRDLLR LVDDLTVQLA LGGLLPGDRV ALCAASNIEF VVGLLAASRA GLIVVPLDPA LPVNEQCIRS QAAGVRVTLV DSLALEGVSD QRAATMRYWP IAVSYGSVTG ASEGSLLVHL DDTAALHPVT STPDGLRHDD AMIMFTGGTT GLPKMVPWTD GNIAGSVHAI ITAYQLGPQD ATVVVMPLYH GHGLIAALLS TLASGGVVLL PARARFSART FWDDIDAVAA XWYTAAPAIH RILLELASTQ SFRSKRAKLR FIRSCSAPLT QETAQALREE FLAPVICAFG MTEATHQVTT TNIKWFGQGE NPTVTNGLVG QSTGVQIRIV GSDGQPLPPD TVGEVWLRGS TVVRGYLGDP AITAANFTHG WLRTGDLGSL SVTGDLRIRG RIKELINRSG EKISPERVEG VLASHHNVME VAVFGDPDKV YGETVTAVIV PREVIAPTPS ELAVFCRDRL AAFEVPTRFQ EASALPHTAK GSLDRRA
Validated NT Sequence
acggcgcggc ggtcgagcga acctttggcg gtgtgcggaa gcgcgctcgc ctcctgaaat ctggtgggca cttcgaaggc cgccaaccga tcacggcaga acaccgcaag ctccgacgga gtcggtgcta tgacctcgcg aggaacaatc actgcggtca ctgtctcccc gtacaccttg tcaggatcac caaataccgc tacctccata acgttgtggt gactggccaa tacaccttcg acgcgctccg gcgagatctt ctcgccggag cggtttatga gttccttgat ccggccacgg atcctcaggt caccggttac cgacagtgac cccagatcac cggtacgcaa ccatccgtgg gtgaagttcg ctgcggttat cgccggatca cccagatacc cacgcaccac tgtggagcca cgcaaccaaa cctcccctac ggtatccggg gggaggggtt gaccgtcgga cccgacgatg cggatctgta cgccagttga ctgaccgaca agaccgtttg ttacggtggg attctcccct tggccgaacc attttatatt tgtggttgtc acctggtgag tggcctcggt catgccgaaa gcacatatca ccggcgccaa gaactcttca cgcagcgctt gcgccgtttc ctgggtaagg ggcgcactgc aactgcggat aaagcggagt ttggctcgct tactccggaa agattgagta gaagcgagtt ccaacaagat ccgatgaatc gccggcgcgg ccgtgtacca annngccgca acggcatcga tgtcgtccca gaatgtgcgc gccgagaacc gcgcgcgagc agggagcaac accacacctc cagatgccag ggtcgacagc aacgcagcaa tgagcccatg accatggtag aggggcatca ccacgacagt cgcatcctgt gggccgagct ggtacgcggt aatgatggcg tgaacggaac cggcgatatt cccgtctgtc cacgggacca tcttcggcaa acccgtcgtt cctccagtga acatgatcat agcgtcatcg tgccggagcc cgtcaggcgt cgaagtgact ggatgcaacg ccgcagtgtc gtccaggtgg accaacaagc tgccttccga agcgcctgtg acgctaccgt aggaaaccgc aatcggccag tagcgcatcg tcgctgctcg ctggtcgcta acgccttcaa gggcaagact gtccaccaat gtcactcgca cgcccgctgc ttggctccgg atgcattgct cattgacagg tagagccgga tctaacggca ccacaatcag gccggctcgc gacgccgcta gtaagcctac aacgaattca atgttgctag ccgcgcacag cgctacccga tcgccaggca gcaggccacc cagcgccagc tggacggtca aatcatcgac cagtcggagc aaatctcggt agctcaccac tatgcgattg tcggtcacga caagcgctgg cgcgcttgga ttccgaattg ctgccgcctc gaccaagctg gcgatactcg ggctttcgaa ttggccagtc agcctcgcat ctatctgttc aaccggagct ctgctgctgg taatcgactc cgatttcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMK SESITSSRAP VEQIDARLTG QFESPSIASL VEAAAIRNPS APALVVTDNR IVVSYRDLLR LVDDLTVQLA LGGLLPGDRV ALCAASNIEF VVGLLAASRA GLIVVPLDPA LPVNEQCIRS QAAGVRVTLV DSLALEGVSD QRAATMRYWP IAVSYGSVTG ASEGSLLVHL DDTAALHPVT STPDGLRHDD AMIMFTGGTT GLPKMVPWTD GNIAGSVHAI ITAYQLGPQD ATVVVMPLYH GHGLIAALLS TLASGGVVLL PARARFSART FWDDIDAVAA TWYTAAPAIH RILLELASTQ SFRSKRAKLR FIRSCSAPLT QETAQALREE FLAPVICAFG MTEATHQVTT TNIKWFGQGE NPTVTNGLVG QSTGVQIRIV GSDGQPLPPD TVGEVWLRGS TVVRGYLGDP AITAANFTHG WLRTGDLGSL SVTGDLRIRG RIKELINRSG EKISPERVEG VLASHHNVME VAVFGDPDKV YGETVTAVIV PREVIAPTPS ELAVFCRDRL AAFEVPTRFQ EASALPHTAK GSLDRRAVAE QFAHRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaat cggagtcgat taccagcagc agagctccgg ttgaacagat agatgcgagg ctgactggcc aattcgaaag cccgagtatc gccagcttgg tcgaggcggc agcaattcgg aatccaagcg cgccagcgct tgtcgtgacc gacaatcgca tagtggtgag ctaccgagat ttgctccgac tggtcgatga tttgaccgtc cagctggcgc tgggtggcct gctgcctggc gatcgggtag cgctgtgcgc ggctagcaac attgaattcg ttgtaggctt actagcggcg tcgcgagccg gcctgattgt ggtgccgtta gatccggctc tacctgtcaa tgagcaatgc atccggagcc aagcagcggg cgtgcgagtg acattggtgg acagtcttgc ccttgaaggc gttagcgacc agcgagcagc gacgatgcgc tactggccga ttgcggtttc ctacggtagc gtcacaggcg cttcggaagg cagcttgttg gtccacctgg acgacactgc ggcgttgcat ccagtcactt cgacgcctga cgggctccgg cacgatgacg ctatgatcat gttcactgga ggaacgacgg gtttgccgaa gatggtcccg tggacagacg ggaatatcgc cggttccgtt cacgccatca ttaccgcgta ccagctcggc ccacaggatg cgactgtcgt ggtgatgccc ctctaccatg gtcatgggct cattgctgcg ttgctgtcga ccctggcatc tggaggtgtg gtgttgctcc ctgctcgcgc gcggttctcg gcgcgcacat tctgggacga catcgatgcc gttgcggcta cttggtacac ggccgcgccg gcgattcatc ggatcttgtt ggaactcgct tctactcaat ctttccggag taagcgagcc aaactccgct ttatccgcag ttgcagtgcg ccccttaccc aggaaacggc gcaagcgctg cgtgaagagt tcttggcgcc ggtgatatgt gctttcggca tgaccgaggc cactcaccag gtgacaacca caaatataaa atggttcggc caaggggaga atcccaccgt aacaaacggt cttgtcggtc agtcaactgg cgtacagatc cgcatcgtcg ggtccgacgg tcaacccctc cccccggata ccgtagggga ggtttggttg cgtggctcca cagtggtgcg tgggtatctg ggtgatccgg cgataaccgc agcgaacttc acccacggat ggttgcgtac cggtgatctg gggtcactgt cggtaaccgg tgacctgagg atccgtggcc ggatcaagga actcataaac cgctccggcg agaagatctc gccggagcgc gtcgaaggtg tattggccag tcaccacaac gttatggagg tagcggtatt tggtgatcct gacaaggtgt acggggagac agtgaccgca gtgattgttc ctcgcgaggt catagcaccg actccgtcgg agcttgcggt gttctgccgt gatcggttgg cggccttcga agtgcccacc agatttcagg aggcgagcgc gcttccgcac accgccaaag gttcgctcga ccgccgcgcc gtggccgaac agttcgctca tcgggggtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at