MyleA.00556.a

Succinyl-CoA ligase [ADP-forming] subunit alpha

CENTER ID: MyleA.00556.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00556.a.A1.GE29222 Full length( MyleA.00556.a ) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|561304.4.peg.237
RefSeq: YP_002502873.1
UniProt: A0A0H3MTQ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIFLTKDNT VIVQGITGSE AAVHTARMLK AGTQIVGGVN ARKAGTNVTH EDKHGRIITL PVFGTVAEAI ENTGADVSII FVPPRFAKEA IIEAVEAEIP LLVIITEGIP VQDSVYAWAY NLNKGTKTRI IGPNCPGIIT PGQSLVGITP ATITGAGPIG LVSKSGTLTY QMMFELSDFG FSTAIGIGGD PVIGTTHIDA IEAFEQDPDT KIIVMIGEIG GDAEERAADY IKANVSKPVV GYVAGFTAPE GKTMGHAGAI VSGSSGTAAV KKDALEAAGV KVGKTPSETA VLVREILCTL
NT Sequence
ATGGCGATTT TCCTCACTAA AGACAACACA GTCATCGTCC AGGGTATCAC TGGCAGCGAG GCTGCCGTTC ACACCGCCCG CATGCTCAAG GCGGGAACCC AAATCGTGGG CGGCGTCAAT GCCCGCAAGG CAGGCACCAA TGTAACGCAT GAGGACAAAC ACGGCCGGAT CATCACACTG CCAGTGTTTG GCACCGTGGC CGAAGCCATC GAAAACACCG GCGCCGACGT CTCGATCATA TTTGTGCCGC CGCGGTTTGC CAAAGAAGCC ATTATCGAGG CCGTCGAAGC AGAAATTCCG CTGCTGGTCA TCATCACTGA GGGAATTCCG GTGCAAGACA GTGTATATGC ATGGGCCTAC AATCTCAACA AAGGTACTAA GACTCGCATA ATCGGCCCGA ACTGCCCCGG TATCATTACT CCCGGCCAGT CGTTGGTTGG CATTACTCCA GCTACCATCA CCGGAGCAGG TCCGATTGGT TTGGTGTCTA AGTCGGGCAC CTTGACCTAC CAGATGATGT TCGAGCTCAG CGATTTCGGC TTCTCGACCG CAATCGGTAT CGGTGGTGAC CCGGTGATTG GTACCACGCA CATCGACGCC ATCGAAGCCT TCGAACAGGA TCCCGACACC AAAATCATCG TCATGATCGG CGAGATCGGG GGGGATGCCG AAGAGCGTGC GGCAGACTAC ATCAAAGCTA ACGTGTCCAA GCCCGTTGTA GGATACGTCG CGGGATTCAC TGCGCCGGAA GGTAAGACGA TGGGCCATGC TGGTGCCATC GTGTCCGGCT CGTCGGGCAC TGCGGCTGTC AAGAAGGACG CCTTAGAGGC AGCGGGAGTC AAAGTCGGTA AGACTCCGTC GGAGACGGCT GTGTTGGTCC GCGAGATTCT GTGCACCCTG
Details for MyleA.00556.a.A1.GE29222
HARVESTED ON: 4/13/2010
SEQUENCED ON: 4/24/2010
EXPECTED MW: 33kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMAIFLTK DNTVIVQGIT GSEAAVHTAR MLKAGTQIVG GVNARKAGTN VTHEDKHGRI ITLPVFGTVA EAIENTGADV SIIFVPPRFA KEAIIEAVEA EIPLLVIITE GIPVQDSVYA WAYNLNKGTK TRIIGPNCPG IITPGQSLVG ITPATITGAG PIGLVSKSGT LTYQMMFELS DFGFSTAIGI GGDPVIGTTH IDAIEAFEQD PDTKIIVMIG EIGGDAEERA ADYIKANVSK PVVGYVAGFT APEGKTMGHA GAIVSGSSGT AAVKKDALEA AGVKVGKTPS ETAVLVREIL CTL
Validated NT Sequence
catcatatgg gtaccctgga agctcagacc cagggtcctg gttcgatggc gattttcctc actaaagaca acacagtcat cgtccagggt atcactggca gcgaggctgc cgttcacacc gcccgcatgc tcaaggcggg aacccaaatc gtgggcggcg tcaatgcccg caaggcaggc accaatgtaa cgcatgagga caaacacggc cggatcatca cactgccagt gtttggcacc gtggccgaag ccatcgaaaa caccggcgcc gacgtctcga tcatatttgt gccgccgcgg tttgccaaag aagccattat cgaggccgtc gaagcagaaa ttccgctgct ggtcatcatc actgagggaa ttccggtgca agacagtgta tatgcatggg cctacaatct caacaaaggt actaagactc gcataatcgg cccgaactgc cccggtatca ttactcccgg ccagtcgttg gttggcatta ctccagctac catcaccgga gcaggtccga ttggtttggt gtctaagtcg ggcaccttga cctaccagat gatgttcgag ctcagcgatt tcggcttctc gaccgcaatc ggtatcggtg gtgacccggt gattggtacc acgcacatcg acgccatcga agccttcgaa caggatcccg acaccaaaat catcgtcatg atcggcgaga tcggggggga tgccgaagag cgtgcggcag actacatcaa agctaacgtg tccaagcccg ttgtaggata cgtcgcggga ttcactgcgc cggaaggtaa gacgatgggc catgctggtg ccatcgtgtc cggctcgtcg ggcactgcgg ctgtcaagaa ggacgcctta gaggcagcgg gagtcaaagt cggtaagact ccgtcggaga cggctgtgtt ggtccgcgag attctgtgca ccctgtaata a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAIFLTKDN TVIVQGITGS EAAVHTARML KAGTQIVGGV NARKAGTNVT HEDKHGRIIT LPVFGTVAEA IENTGADVSI IFVPPRFAKE AIIEAVEAEI PLLVIITEGI PVQDSVYAWA YNLNKGTKTR IIGPNCPGII TPGQSLVGIT PATITGAGPI GLVSKSGTLT YQMMFELSDF GFSTAIGIGG DPVIGTTHID AIEAFEQDPD TKIIVMIGEI GGDAEERAAD YIKANVSKPV VGYVAGFTAP EGKTMGHAGA IVSGSSGTAA VKKDALEAAG VKVGKTPSET AVLVREILCT L
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgattttc ctcactaaag acaacacagt catcgtccag ggtatcactg gcagcgaggc tgccgttcac accgcccgca tgctcaaggc gggaacccaa atcgtgggcg gcgtcaatgc ccgcaaggca ggcaccaatg taacgcatga ggacaaacac ggccggatca tcacactgcc agtgtttggc accgtggccg aagccatcga aaacaccggc gccgacgtct cgatcatatt tgtgccgccg cggtttgcca aagaagccat tatcgaggcc gtcgaagcag aaattccgct gctggtcatc atcactgagg gaattccggt gcaagacagt gtatatgcat gggcctacaa tctcaacaaa ggtactaaga ctcgcataat cggcccgaac tgccccggta tcattactcc cggccagtcg ttggttggca ttactccagc taccatcacc ggagcaggtc cgattggttt ggtgtctaag tcgggcacct tgacctacca gatgatgttc gagctcagcg atttcggctt ctcgaccgca atcggtatcg gtggtgaccc ggtgattggt accacgcaca tcgacgccat cgaagccttc gaacaggatc ccgacaccaa aatcatcgtc atgatcggcg agatcggggg ggatgccgaa gagcgtgcgg cagactacat caaagctaac gtgtccaagc ccgttgtagg atacgtcgcg ggattcactg cgccggaagg taagacgatg ggccatgctg gtgccatcgt gtccggctcg tcgggcactg cggctgtcaa gaaggacgcc ttagaggcag cgggagtcaa agtcggtaag actccgtcgg agacggctgt gttggtccgc gagattctgt gcaccctgaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa