SSGCID: Target Page

MyleA.00519.a

Putative serine acetyltransferase

CENTER ID:	MyleA.00519.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00519.a.A1.GE29220	Full length( MyleA.00519.a )		1	227

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1557
RefSeq:	YP_002503262.1
UniProt:	A0A0H3MYW1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MFAAIRRDIQ AARQRDPAQP TVLEVICCYP GVHAVWGHRI SHWLWNRRAR LAARAFAELT RILTGVDIHP GAVLGAGLFI DHATGVVIGE TAEVGDDVTI FHGVTLGGTG RETGKRHPTI GDRVTIGAGA KVLGAIKIGE DSRIGANAVV VKEVPASAVA VGVPGQIISS DSPANGDDSV LPDFVGVSLQ SLLTRVAKLE AEDGGSQTYR VIRLPEAGVW HGEDFSI

NT Sequence

ATGTTTGCGG CAATCCGGCG TGATATCCAG GCAGCAAGAC AGCGAGATCC GGCACAGCCC ACGGTGCTGG AGGTCATCTG CTGCTACCCA GGCGTGCACG CCGTCTGGGG TCATCGAATC AGTCACTGGT TGTGGAATCG TCGCGCCAGA CTGGCCGCGC GGGCGTTCGC CGAACTCACC CGCATCCTGA CTGGGGTCGA CATCCACCCC GGTGCCGTGC TCGGAGCCGG CCTGTTCATC GATCACGCGA CCGGCGTGGT GATCGGGGAA ACCGCGGAAG TGGGCGATGA CGTCACCATC TTCCATGGAG TCACTCTCGG CGGCACCGGC CGGGAAACGG GTAAACGTCA CCCAACCATC GGGGATCGAG TAACCATCGG CGCCGGCGCC AAGGTCCTCG GTGCCATCAA GATCGGCGAG GACAGCCGGA TTGGCGCCAA CGCAGTCGTG GTCAAGGAGG TCCCAGCCAG CGCTGTGGCC GTCGGGGTTC CCGGACAAAT CATCAGCAGC GACAGCCCGG CCAACGGGGA CGATTCTGTG CTGCCCGACT TCGTGGGCGT CAGCCTGCAA TCCCTGCTCA CCAGGGTGGC CAAGCTGGAA GCCGAAGACG GCGGTTCGCA AACCTACCGC GTCATCCGGC TACCCGAAGC CGGGGTTTGG CACGGCGAGG ACTTCTCAAT C

Details for MyleA.00519.a.A1.GE29220

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	26kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMFAAIRRDI QAARQRDPAQ PTVLEVICCY PGVHAVWGHR ISHWLWNRRA RLAARAFAEL TRILTGVDIH PGAVLGAGLF IDHATGVVIG ETAEVGDDVT IFHGVTLGGT GRETGKRHPT IGDRVTIGAG AKVLGAIKIG EDSRIGANAV VVKEVPASAV AVGVPGQIIS SDSPANGDDS VLPDFVGVSL QSLLTRVAKL EAEDGGSQTY RVIRLPEAGV WHGEDFSI

Validated NT Sequence

ttattagatt gagaagtcct cgccgtgcca aaccccggct tcgggtagcc ggatgacgcg gtaggtttgc gaaccgccgt cttcggcttc cagcttggcc accctggtga gcagggattg caggctgacg cccacgaagt cgggcagcac agaatcgtcc ccgttggccg ggctgtcgct gctgatgatt tgtccgggaa ccccgacggc cacagcgctg gctgggacct ccttgaccac gactgcgttg gcgccaatcc ggctgtcctc gccgatcttg atggcaccga ggaccttggc gccggcgccg atggttactc gatccccgat ggttgggtga cgtttacccg tttcccggcc ggtgccgccg agagtgactc catggaagat ggtgacgtca tcgcccactt ccgcggtttc cccgatcacc acgccggtcg cgtgatcgat gaacaggccg gctccgagca cggcaccggg gtggatgtcg accccagtca ggatgcgggt gagttcggcg aacgcccgcg cggccagtct ggcgcgacga ttccacaacc agtgactgat tcgatgaccc cagacggcgt gcacgcctgg gtagcagcag atgacctcca gcaccgtggg ctgtgccgga tctcgctgtc ttgctgcctg gatatcacgc cggattgccg caaacatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtttgcggca atccggcgtg atatccaggc agcaagacag cgagatccgg cacagcccac ggtgctggag gtcatctgct gctacccagg cgtgcacgcc gtctggggtc atcgaatcag tcactggttg tggaatcgtc gcgccagact ggccgcgcgg gcgttcgccg aactcacccg catcctgact ggggtcgaca tccaccccgg tgccgtgctc ggagccggcc tgttcatcga tcacgcgacc ggcgtggtga tcggggaaac cgcggaagtg ggcgatgacg tcaccatctt ccatggagtc actctcggcg gcaccggccg ggaaacgggt aaacgtcacc caaccatcgg ggatcgagta accatcggcg ccggcgccaa ggtcctcggt gccatcaaga tcggcgagga cagccggatt ggcgccaacg cagtcgtggt caaggaggtc ccagccagcg ctgtggccgt cggggttccc ggacaaatca tcagcagcga cagcccggcc aacggggacg attctgtgct gcccgacttc gtgggcgtca gcctgcaatc cctgctcacc agggtggcca agctggaagc cgaagacggc ggttcgcaaa cctaccgcgt catccggcta cccgaagccg gggtttggca cggcgaggac ttctcaatca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a