SSGCID: Target Page

MyleA.00476.a

Probable 3-methyl-2-oxobutanoate hydroxymethyltransferase PanB

CENTER ID:	MyleA.00476.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00476.a.A1.GE34166	full length		1	286

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3092
RefSeq:	YP_002503756.1
UniProt:	A0A0H3MR94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSEQNVNVYG ADPSTSSIQS IQRTKIRTKH LQKMKAEGHK WAMLTAYDYS TARVFDEAGI PVLLVGDSAA NVVYGYDTTV PVSADELLPL VRGVVRGAGH ALVVADLPFG SYEPGPTAAL AVATRFMKEG GAHAVKLEGG QRVAEQIACL TAAGIPVMAH IGFTPQSVNS LGGFRVQGRG GDAEQTVADA VAVAEAGAFS VVMEMVPTEL ATQITGKLTI PTIGIGAGLN CDAQVLVWQD MAGLSSGKVA RFVKQYADIA GELRRAAMQY AEEVASAVFP AEEHCF

NT Sequence

atgtctgagc agaatgttaa tgtttatggg gctgatcctt ctacatcctc cattcaatca atacagcgca ccaaaatacg caccaagcat ctccaaaaaa tgaaggccga aggccacaag tgggccatgc tgacagccta tgattattcg actgctcggg ttttcgacga agccggtatc ccggtgttgc tggtgggtga ttcagcggcc aacgtggtgt acggctacga caccacggtg ccggtttcgg ccgacgaact actcccgctg gttcgcggcg tggtgcgggg tgcggggcac gctctggtcg tcgccgacct gccattcggc agctacgaac ccggacccac tgccgcgctg gctgtcgcca cccgatttat gaaggagggc ggtgcacacg ccgtaaaact cgaaggcggc cagcgggtag ctgaacaaat cgcgtgcctg accgcagcag gcattccggt aatggcgcac atcggattca caccgcaaag cgtcaacagc ctcggcggtt tccgggtaca gggccgcggc ggcgacgccg aacaaaccgt cgctgatgcc gttgccgttg ctgaagccgg agcattttcc gtcgtaatgg aaatggtgcc gaccgagtta gccacccaga tcaccggcaa attaaccatt ccgacaatcg ggatcggtgc gggtcttaac tgcgacgccc aggtcttagt ttggcaagat atggccggtt taagcagcgg taaggtggca cgtttcgtca agcagtatgc tgatatcgct ggcgagttgc ggcgtgccgc aatgcagtac gcagaggaag tggccagcgc ggttttccct gctgaggagc actgcttctg a

Details for MyleA.00476.a.A1.GE34166

HARVESTED ON:	12/6/2011
SEQUENCED ON:	10/23/2013
EXPECTED MW:	32kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSEQNVNVY GADPSTSSIQ SIQRTKIRTK HLQKMKAEGH KWAMLTAYDY STARVFDEAG IPVLLVGDSA ANVVYGYDTT VPVSADELLP LVRGVVRGAG HALVVADLPF GSYEPGPTAA LAVATRFMKE GGAHAVKLEG GQRVAEQIAC LTAAGIPVMA HIGFTPQSVN SLGGFRVQGR GGDAEQTVAD AVAVAEAGAF SVVMEMVPTE LATQITGKLT IPTIGIGAGL NCDAQVLVWQ DMAGLSSGKV ARFVKQYADI AGELRRAAMQ YAXEXASAVF PXE

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgtctg agcagaatgt taatgtttat ggggctgatc cttctacatc ctccattcaa tcaatacagc gcaccaaaat acgcaccaag catctccaaa aaatgaaggc cgaaggccac aagtgggcca tgctgacagc ctatgattat tcgactgctc gggttttcga cgaagccggt atcccggtgt tgctggtggg tgattcagcg gccaacgtgg tgtacggcta cgacaccacg gtgccggttt cggccgacga actactcccg ctggttcgcg gcgtggtgcg gggtgcgggg cacgctctgg tcgtcgccga cctgccattc ggcagctacg aacccggacc cactgccgcg ctggctgtcg ccacccgatt tatgaaggag ggcggtgcac acgccgtaaa actcgaaggc ggccagcggg tagctgaaca aatcgcgtgc ctgaccgcag caggcattcc ggtaatggcg cacatcggat tcacaccgca aagcgtcaac agcctcggcg gtttccgggt acagggccgc ggcggcgacg ccgaacaaac cgtcgctgat gccgttgccg ttgctgaagc cggagcattt tccgtcgtaa tggaaatggt gccgaccgag ttagccaccc agatcaccgg caaattaacc attccgacaa tcgggatcgg tgcgggtctt aactgcgacg cccaggtctt agtttggcaa gatatggccg gtttaagcag cggtaaggtg gcacgtttcg tcaagcagta tgctgatatc gctggcgagt tgcggcgtgc cgcnatgcag tacgcanang aantggccag cgcggttttc cctgntgagg a

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtctgagcag aatgttaatg tttatggggc tgatccttct acatcctcca ttcaatcaat acagcgcacc aaaatacgca ccaagcatct ccaaaaaatg aaggccgaag gccacaagtg ggccatgctg acagcctatg attattcgac tgctcgggtt ttcgacgaag ccggtatccc ggtgttgctg gtgggtgatt cagcggccaa cgtggtgtac ggctacgaca ccacggtgcc ggtttcggcc gacgaactac tcccgctggt tcgcggcgtg gtgcggggtg cggggcacgc tctggtcgtc gccgacctgc cattcggcag ctacgaaccc ggacccactg ccgcgctggc tgtcgccacc cgatttatga aggagggcgg tgcacacgcc gtaaaactcg aaggcggcca gcgggtagct gaacaaatcg cgtgcctgac cgcagcaggc attccggtaa tggcgcacat cggattcaca ccgcaaagcg tcaacagcct cggcggtttc cgggtacagg gccgcggcgg cgacgccgaa caaaccgtcg ctgatgccgt tgccgttgct gaagccggag cattttccgt cgtaatggaa atggtgccga ccgagttagc cacccagatc accggcaaat taaccattcc gacaatcggg atcggtgcgg gtcttaactg cgacgcccag gtcttagttt ggcaagatat ggccggttta agcagcggta aggtggcacg tttcgtcaag cagtatgctg atatcgctgg cgagttgcgg cgtgccgcaa tgcagtacgc agaggaagtg gccagcgcgg ttttccctgc tgaggagcac tgcttctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa