SSGCID: Target Page

MyleA.00429.a

6-phosphofructokinase

CENTER ID:	MyleA.00429.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00429.a.A1.GE29215	Full length( MyleA.00429.a )		1	343

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3220
RefSeq:	YP_002503800.1
UniProt:	A0A0H3MQZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRIGILTGGG DCPGLNAVIR AIVRTCDARY GSSVVGFQDG WRGLLENRRM QLCNDDRNDR LLAKGGTMLG TAHVHPDKLR AGLHQIKQTL DDNGIDVLIP IGGEGTLTAA HWLSQEDVPV VGVPKTIDND IDCTDVTFGH DTALTVATEA IDRLHSTAES HQRVMLVEVM GRHAGWIALS SGLASGAHMT LIPEQPFDVE EVCCLVKRRF QRGDSHFICV VAEGAKPVPG SITLRQGGMD EFGHERFTGV AAQLGAEVEK RINKDVRVTV LGHVQRGGTP TAFDRVLATR FGVNAADASH AGEYGQMVSL RGQDIGRVPL EDAVRQLKLV PESRYDDAAA FFG

NT Sequence

ATGAGGATCG GAATTCTCAC GGGGGGCGGC GACTGTCCTG GTCTTAACGC CGTTATCCGG GCGATTGTGC GCACCTGTGA CGCCCGGTAC GGTTCGTCGG TAGTCGGGTT TCAGGACGGC TGGCGTGGGT TGCTGGAGAA TCGGCGCATG CAGCTGTGCA ACGACGACCG GAATGACCGT CTGTTAGCCA AAGGCGGAAC CATGCTGGGC ACTGCCCACG TGCACCCCGA CAAGCTGCGG GCCGGGCTGC ATCAGATCAA GCAGACCCTG GATGACAATG GCATCGACGT GCTCATCCCG ATCGGCGGCG AAGGCACGCT GACGGCCGCG CACTGGCTGT CGCAGGAAGA CGTTCCTGTG GTCGGCGTGC CGAAGACGAT CGACAACGAC ATCGACTGCA CCGACGTGAC CTTTGGCCAC GACACCGCGC TGACGGTGGC TACCGAAGCC ATTGACCGGT TGCACAGTAC CGCCGAATCT CACCAACGTG TGATGCTGGT GGAGGTGATG GGTCGGCATG CCGGCTGGAT AGCGCTGAGT TCCGGGCTGG CTTCCGGTGC GCACATGACG CTGATTCCCG AACAGCCCTT CGATGTCGAG GAAGTGTGCT GCCTCGTGAA ACGACGTTTT CAGCGCGGGG ATTCGCATTT CATTTGCGTG GTCGCTGAGG GTGCCAAGCC GGTCCCAGGC TCGATCACGT TGCGCCAAGG TGGGATGGAT GAATTTGGTC ACGAGCGTTT CACTGGGGTG GCAGCGCAAT TGGGCGCTGA GGTCGAGAAG CGGATCAACA AGGACGTGCG AGTGACTGTG CTGGGTCATG TCCAACGCGG TGGTACACCG ACGGCCTTCG ATCGGGTGCT GGCCACCCGG TTCGGGGTTA ATGCTGCCGA CGCCTCGCAC GCCGGCGAGT ACGGCCAGAT GGTGTCACTG CGTGGGCAAG ACATCGGTCG CGTACCGCTG GAGGATGCGG TGCGTCAGCT CAAATTGGTG CCCGAAAGCC GTTACGACGA CGCCGCCGCC TTCTTCGGT

Details for MyleA.00429.a.A1.GE29215

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

aaccgaagaa ggcggcggcg tcgtcgtaac ggctttcggg caccaatttg agctgacgca ccgcatcctc cagcggtacg cgaccgatgt cttgcccacg cagtgacacc atctggccgt actcgccggc gtgcgaggcg tcggcagcat taaccccgaa ccgggtggcc agcacccgat cgaaggccgt cggtgtacca ccgcgttgga catgacccag cacagtcact cgcacgtcct tgttgatccg cttctcgacc tcagcgccca attgcgctgc caccccagtg aaacgctcgt gaccaaattc atccatccca ccttggcgca acgtgatcga gcctgggacc ggcttggcac cctcagcgac cacgcaaatg aaatgcgaat ccccgcgctg aaaacgtcgt ttcacgaggc agcacacttc ctcgacatcg aagggctgtt cgggaatcag cgtcatgtgc gcaccggaag ccagcccgga actcagcgct atccagccgg catgccgacc catcacctcc accagcatca cacgttggtg agattcggcg gtactgtgca accggtcaat ggcttcggta gccaccgtca gcgcggtgtc gtggccaaag gtcacgtcgg tgcagtcgat gtcgttgtcg atcgtcttcg gcacgccgac cacaggaacg tcttcctgcg acagccagtg cgcggccgtc agcgtgcctt cgccgccgat cgggatgagc acgtcgatgc cattgtcatc cagggtctgc ttgatctgat gcagcccggc ccgcagcttg tcggggtgca cgtgggcagt gcccagcatg gttccgcctt tggctaacag acggtcattc cggtcgtcgt tgcacagctg catgcgccga ttctccagca acccacgcca gccgtcctga aacccgacta ccgacgaacc gtaccgggcg tcacaggtgc gcacaatcgc ccggataacg gcgttaagac caggacagtc gccgcccccc gtgagaattc cgatcctcat cgaaccagga ccctgggtct gagcttccag ggt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMRIGILTGG GDCPGLNAVI RAIVRTCDAR YGSSVVGFQD GWRGLLENRR MQLCNDDRND RLLAKGGTML GTAHVHPDKL RAGLHQIKQT LDDNGIDVLI PIGGEGTLTA AHWLSQEDVP VVGVPKTIDN DIDCTDVTFG HDTALTVATE AIDRLHSTAE SHQRVMLVEV MGRHAGWIAL SSGLASGAHM TLIPEQPFDV EEVCCLVKRR FQRGDSHFIC VVAEGAKPVP GSITLRQGGM DEFGHERFTG VAAQLGAEVE KRINKDVRVT VLGHVQRGGT PTAFDRVLAT RFGVNAADAS HAGEYGQMVS LRGQDIGRVP LEDAVRQLKL VPESRYDDAA AFFG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaggatcgga attctcacgg ggggcggcga ctgtcctggt cttaacgccg ttatccgggc gattgtgcgc acctgtgacg cccggtacgg ttcgtcggta gtcgggtttc aggacggctg gcgtgggttg ctggagaatc ggcgcatgca gctgtgcaac gacgaccgga atgaccgtct gttagccaaa ggcggaacca tgctgggcac tgcccacgtg caccccgaca agctgcgggc cgggctgcat cagatcaagc agaccctgga tgacaatggc atcgacgtgc tcatcccgat cggcggcgaa ggcacgctga cggccgcgca ctggctgtcg caggaagacg ttcctgtggt cggcgtgccg aagacgatcg acaacgacat cgactgcacc gacgtgacct ttggccacga caccgcgctg acggtggcta ccgaagccat tgaccggttg cacagtaccg ccgaatctca ccaacgtgtg atgctggtgg aggtgatggg tcggcatgcc ggctggatag cgctgagttc cgggctggct tccggtgcgc acatgacgct gattcccgaa cagcccttcg atgtcgagga agtgtgctgc ctcgtgaaac gacgttttca gcgcggggat tcgcatttca tttgcgtggt cgctgagggt gccaagccgg tcccaggctc gatcacgttg cgccaaggtg ggatggatga atttggtcac gagcgtttca ctggggtggc agcgcaattg ggcgctgagg tcgagaagcg gatcaacaag gacgtgcgag tgactgtgct gggtcatgtc caacgcggtg gtacaccgac ggccttcgat cgggtgctgg ccacccggtt cggggttaat gctgccgacg cctcgcacgc cggcgagtac ggccagatgg tgtcactgcg tgggcaagac atcggtcgcg taccgctgga ggatgcggtg cgtcagctca aattggtgcc cgaaagccgt tacgacgacg ccgccgcctt cttcggtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa