SSGCID: Target Page

MyleA.00384.a

Putative cytoplasmic peptidase

CENTER ID:	MyleA.00384.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00384.a.A1.GE29210	Full length( MyleA.00384.a )		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.905
RefSeq:	YP_002503077.1
UniProt:	A0A0H3MPW5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTHSQRRDNL KSQIATTGLD AMLVTCLINV RYLSGFTGSN GALLVFADER GAVLATDGRY RTQAAQQVPE LEVAIEQSCG RYLAGRAADD GVCILGFESH VVTVDGLDAL TGELEGRHTE LVRAPGTVEV LREVKDAGEL ALLRLAAEAA DAALTELVAR GGVRPGLTER EVGRELEGLM LDHGADALSF ETIVAAGPNS AIPHHRPTDT VLAAGDFVKI DFGALVAGYH SDMTRTFVLG PAADWQLEIY QLVADAQRAG RKALRPGANL RDVDAAARQL IVDAGYGQQF SHGLGHGVGL EIHEAPGIGT TSAGTLLAGS VVTVEPGVYL SGRGGVRIED TLVVAAKGTS ETPITSRPTG FIPELLTRFP KELAIL

NT Sequence

ATGACACATT CCCAGCGTCG AGACAATCTC AAGTCACAAA TCGCGACCAC CGGGCTGGAC GCGATGCTGG TGACGTGCCT GATCAATGTG CGCTACCTGT CAGGTTTCAC TGGATCGAAC GGTGCTTTGC TGGTGTTCGC CGACGAACGC GGCGCTGTGC TCGCCACCGA CGGCCGTTAC CGGACTCAGG CTGCGCAGCA AGTACCCGAG CTCGAGGTCG CCATCGAGCA GTCCTGCGGG CGCTATTTGG CTGGCCGGGC AGCCGACGAC GGCGTGTGCA TACTAGGCTT TGAAAGCCAT GTGGTCACGG TCGACGGCCT GGACGCGTTG ACCGGCGAGC TCGAAGGCCG GCACACCGAG TTGGTGCGTG CCCCTGGAAC CGTGGAGGTG CTGCGCGAGG TCAAGGATGC TGGTGAGCTT GCGTTGCTGC GGTTGGCCGC CGAGGCGGCT GATGCCGCGC TGACGGAGCT GGTGGCGCGT GGCGGAGTGC GGCCCGGTCT TACCGAACGT GAGGTGGGCC GCGAGTTGGA GGGGTTGATG CTCGACCACG GCGCTGACGC GCTGTCGTTC GAGACGATTG TGGCCGCCGG GCCGAATTCG GCTATCCCGC ACCACCGGCC GACCGACACG GTGCTGGCGG CGGGCGATTT CGTCAAGATC GACTTTGGTG CGCTAGTAGC TGGATATCAC TCCGACATGA CTCGCACCTT TGTGCTGGGC CCGGCTGCTG ACTGGCAGCT GGAAATCTAT CAACTGGTCG CCGATGCGCA ACGTGCAGGT CGGAAGGCGT TGCGGCCGGG TGCCAACCTG CGTGACGTGG ACGCAGCGGC ACGCCAACTG ATCGTCGACG CGGGCTACGG CCAACAGTTC AGCCACGGTT TGGGCCACGG CGTGGGCCTG GAAATCCACG AAGCGCCGGG TATCGGGACT ACCTCTGCCG GTACACTACT CGCAGGTTCC GTCGTGACCG TGGAGCCCGG TGTCTACTTA TCTGGCCGAG GCGGTGTCCG GATCGAGGAT ACACTGGTGG TGGCCGCTAA AGGAACGTCA GAGACGCCGA TAACTTCTAG ACCCACCGGG TTCATTCCGG AATTGTTGAC CCGGTTCCCT AAAGAACTGG CAATCCTG

Details for MyleA.00384.a.A1.GE29210

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	41kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

Validated NT Sequence

accctggaag ctcagaccca gggtcctggt tcgatgacac attcccagcg tcgagacaat ctcaagtcac aaatcgcgac caccgggctg gacgcgatgc tggtgacgtg cctgatcaat gtgcgctacc tgtcaggttt cactggatcg aacggtgctt tgctggtgtt cgccgacgaa cgcggcgctg tgctcgccac cgacggccgt taccggactc aggctgcgca gcaagtaccc gagctcgagg tcgccatcga gcagtcctgc gggcgctatt tggctggccg ggcagccgac gacggcgtgt gcatactagg ctttgaaagc catgtggtca cggtcgacgg cctggacgcg ttgaccggcg agctcgaagg ccggcacacc gagttggtgc gtgcccctgg aaccgtggag gtgctgcgcg aggtcaagga tgctggtgag cttgcgttgc tgcggttggc cgccgaggcg gctgatgccg cgctgacgga gctggtggcg cgtggcggag tgcggcccgg tcttaccgaa cgtgaggtgg gccgcgagtt ggaggggttg atgctcgacc acggcgctga cgcgctgtcg ttcgagacga ttgtggccgc cgggccgaat tcggctatcc cgcaccaccg gccgaccgac acggtgctgg cggcgggcga tttcgtcaag atcgactttg gtgcgctagt agctggatat cactccgaca tgactcgcac ctttgtgctg ggcccggctg ctgactggca gctggaaatc tatcaactgg tcgccgatgc gcaacgtgca ggtcggaagg cgttgcggcc gggtgccaac ctgcgtgacg tggacgcagc ggcacgccaa ctgatcgtcg acgcgggcta cggccaacag ttcagccacg gtttgggcca cggcgtgggc ctggaaatcc acgaagcgcc gggtatcggg actacctctg ccggtacact actcgcaggt tccgtcgtga ccgtggagcc cggtgtctac ttatctggcc gaggcggtgt ccggatcgag gatacactgg tggtggccgc taaaggaacg tcagagacgc cgataacttc tagacccacc gggttcattc cggaattgtt gacccggttc cctaaagaac tggcaatcct gt

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMTHSQRRDN LKSQIATTGL DAMLVTCLIN VRYLSGFTGS NGALLVFADE RGAVLATDGR YRTQAAQQVP ELEVAIEQSC GRYLAGRAAD DGVCILGFES HVVTVDGLDA LTGELEGRHT ELVRAPGTVE VLREVKDAGE LALLRLAAEA ADAALTELVA RGGVRPGLTE REVGRELEGL MLDHGADALS FETIVAAGPN SAIPHHRPTD TVLAAGDFVK IDFGALVAGY HSDMTRTFVL GPAADWQLEI YQLVADAQRA GRKALRPGAN LRDVDAAARQ LIVDAGYGQQ FSHGLGHGVG LEIHEAPGIG TTSAGTLLAG SVVTVEPGVY LSGRGGVRIE DTLVVAAKGT SETPITSRPT GFIPELLTRF PKELAIL

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacacattcc cagcgtcgag acaatctcaa gtcacaaatc gcgaccaccg ggctggacgc gatgctggtg acgtgcctga tcaatgtgcg ctacctgtca ggtttcactg gatcgaacgg tgctttgctg gtgttcgccg acgaacgcgg cgctgtgctc gccaccgacg gccgttaccg gactcaggct gcgcagcaag tacccgagct cgaggtcgcc atcgagcagt cctgcgggcg ctatttggct ggccgggcag ccgacgacgg cgtgtgcata ctaggctttg aaagccatgt ggtcacggtc gacggcctgg acgcgttgac cggcgagctc gaaggccggc acaccgagtt ggtgcgtgcc cctggaaccg tggaggtgct gcgcgaggtc aaggatgctg gtgagcttgc gttgctgcgg ttggccgccg aggcggctga tgccgcgctg acggagctgg tggcgcgtgg cggagtgcgg cccggtctta ccgaacgtga ggtgggccgc gagttggagg ggttgatgct cgaccacggc gctgacgcgc tgtcgttcga gacgattgtg gccgccgggc cgaattcggc tatcccgcac caccggccga ccgacacggt gctggcggcg ggcgatttcg tcaagatcga ctttggtgcg ctagtagctg gatatcactc cgacatgact cgcacctttg tgctgggccc ggctgctgac tggcagctgg aaatctatca actggtcgcc gatgcgcaac gtgcaggtcg gaaggcgttg cggccgggtg ccaacctgcg tgacgtggac gcagcggcac gccaactgat cgtcgacgcg ggctacggcc aacagttcag ccacggtttg ggccacggcg tgggcctgga aatccacgaa gcgccgggta tcgggactac ctctgccggt acactactcg caggttccgt cgtgaccgtg gagcccggtg tctacttatc tggccgaggc ggtgtccgga tcgaggatac actggtggtg gccgctaaag gaacgtcaga gacgccgata acttctagac ccaccgggtt cattccggaa ttgttgaccc ggttccctaa agaactggca atcctgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa