SSGCID: Target Page

MyleA.00339.a

ALANINE RACEMASE ALR

CENTER ID:	MyleA.00339.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00339.a.A1.GE34163	full length		1	388

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.632
RefSeq:	YP_002502998.1
UniProt:	A0A0H3MPD2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVTPISLRP GVLAEAVVDL GAIDYNVRVL REHAGMAQLM VVLKADAYGH GATQVALAAL AAGAAELGVA TVDEALALRA DGISAPVLAW LHPPGIDFGP ALLADVQIAV SSVRQLDELL DAVRRTGRTA TVTVKADTGL NRNGVVTDQY PAMLTALQRA VVEDAVRLRG LMSHLVYADQ PDNPSNDVQG KRFAALLAQA HEQGLRFEVA HLSNSSATMS RPDLAYDLVR PGIAVYGLSP VPSRGDMGLI PAMTVKCAVA MVKSIRAGEG VSYGHDWIAQ HDTNLALLPV GYADGVFRSL GGRLDVLING KRRPGVGRIC MDQFVVDLGP GPIDVAEGDE AILFGPGARG EPTAQDWADL LGTIHYEVVT SLRGRITRTY REAQTVDR

NT Sequence

ttggctgtta caccgatatc cttgaggcca ggtgtcttgg ccgaagctgt tgtggatctg ggagctattg actacaacgt gcgcgtgttg cgtgagcacg ctggcatggc gcaactgatg gttgtcctca aagccgacgc ctacggtcac ggtgccaccc aggttgccct cgccgcattg gcagccggtg cggccgagct gggtgtggcc accgtcgacg aagcgctcgc attgcgcgcg gacggtatca gtgcaccggt gctggcctgg ctgcatccac ccggaatcga cttcgggccc gcgctgctgg ccgacgtgca gatagcggtg tcctcggtgc gtcagcttga cgagctgctg gatgcggtgc gtcggaccgg tcgtacggcg acggtgaccg tcaaagccga caccgggctg aaccgcaacg gggtcgtcac cgatcaatac ccggcgatgc tgaccgcgtt gcagcgggct gtcgtagaag acgccgtaag gctgcgcggg ttgatgtcgc acttggttta cgctgaccag cccgataatc ccagcaatga cgttcagggc aagcggtttg ctgctctgct cgctcaggcg cacgaacagg gattgcggtt cgaggtggcg catctgtcga attcatcagc tacgatgtct cgtcctgacc tggcttacga cctggtcagg cccggcatcg cggtgtacgg tcttagtcct gtgccttccc gcggtgatat gggactaata cccgcaatga cggtgaaatg cgctgttgcg atggtgaagt ctattcgtgc tggtgaaggt gtttcgtacg gacacgactg gattgcgcag catgacacca atttggcgct gttgccggtt ggatatgcgg acggtgtgtt ccggtcgctg ggcggccggc tggatgtgct gatcaatggc aagcgccggc ctggcgtcgg acggatctgt atggaccagt ttgtcgtcga cttgggtccg gggccaatcg atgtagccga aggcgacgaa gccatcttgt tcggtcccgg cgccagaggc gaacccactg cgcaagattg ggctgatctg cttggaacta tccactatga agtggtcacc agtctacgcg ggcggatcac cagaacgtac cgcgaggccc aaaccgttga cagatga

Details for MyleA.00339.a.A1.GE34163

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	79

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLAVTPISL RPGVLAEAVV DLGAIDYNVR VLREHAGMAQ LMVVLKADAY GHGATQVALA ALAAGAAELG VATVDEALAL RADGISAPVL AWLHPPGIDF GPALLADVQI AVSSVRQLDE LLDAVRRTGR TATVTVKADT GLNRNGVVTD QYPAMLTALQ RAVVEDAVRL RGLMSHLVYA DQPDNPSNDV QGKRFAALLA QAHEQGLRFE VAHLSNSSAT MSRPDLAYDL VRPGIAVYGL SPVPSRGDMG LIPAMTVKCA VAMVKSIRAG EGVSYGHDWX XSMTXIXXXX XXXXXXSXAG XXXX

Validated NT Sequence

ttttntnnnn tttaagaagg aganatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgttggc tgttacaccg atatccttga ggccaggtgt cttggccgaa gctgttgtgg atctgggagc tattgactac aacgtgcgcg tgttgcgtga gcacgctggc atggcgcaac tgatggttgt cctcaaagcc gacgcctacg gtcacggtgc cacccaggtt gccctcgccg cattggcagc cggtgcggcc gagctgggtg tggccaccgt cgacgaagcg ctcgcattgc gcgcggacgg tatcagtgca ccggtgctgg cctggctgca tccacccgga atcgacttcg ggcccgcgct gctggccgac gtgcagatag cggtgtcctc ggtgcgtcag cttgacgagc tgctggatgc ggtgcgtcgg accggtcgta cggcgacggt gaccgtcaaa gccgacaccg ggctgaaccg caacggggtc gtcaccgatc aatacccggc gatgctgacc gcgttgcagc gggctgtcgt agaagacgcc gtaaggctgc gcgggttgat gtcgcacttg gtttacgctg accagcccga taatcccagc aatgacgttc agggcaagcg gtttgctgct ctgctcgctc aggcgcacga acagggattg cggttcgagg tggcgcatct gtcgaattca tcagctacga tgtctcgtcc tgacctggct tacgacctgg tcaggcccgg catcgcggtg tacggtctta gtcctgtgcc ttcccgcggt gatatgggac taatacccgc aatgacggtg aaatgcgctg ttgcnatggt gaagtctatt cgtgctggtg aaggtgtttc gtacggacac gactggnttg ncagcatgac ancnatttng cnctntnnnn nnnatgnnna nnngngttcc gncgctggcg nnntgnngtg ntgatcangn nagcnnnntg cgnngnngnt ctgnnngann antnnncnnn nnntngnnng nnnnnnntgt nnnnaannnn nnnnnnnntn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna nnnnnnnnnn nntnnnnnnn nnnnnnnnnn ncngnnnnnc nnnnnnannn nnnnnnnna

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggctgtt acaccgatat ccttgaggcc aggtgtcttg gccgaagctg ttgtggatct gggagctatt gactacaacg tgcgcgtgtt gcgtgagcac gctggcatgg cgcaactgat ggttgtcctc aaagccgacg cctacggtca cggtgccacc caggttgccc tcgccgcatt ggcagccggt gcggccgagc tgggtgtggc caccgtcgac gaagcgctcg cattgcgcgc ggacggtatc agtgcaccgg tgctggcctg gctgcatcca cccggaatcg acttcgggcc cgcgctgctg gccgacgtgc agatagcggt gtcctcggtg cgtcagcttg acgagctgct ggatgcggtg cgtcggaccg gtcgtacggc gacggtgacc gtcaaagccg acaccgggct gaaccgcaac ggggtcgtca ccgatcaata cccggcgatg ctgaccgcgt tgcagcgggc tgtcgtagaa gacgccgtaa ggctgcgcgg gttgatgtcg cacttggttt acgctgacca gcccgataat cccagcaatg acgttcaggg caagcggttt gctgctctgc tcgctcaggc gcacgaacag ggattgcggt tcgaggtggc gcatctgtcg aattcatcag ctacgatgtc tcgtcctgac ctggcttacg acctggtcag gcccggcatc gcggtgtacg gtcttagtcc tgtgccttcc cgcggtgata tgggactaat acccgcaatg acggtgaaat gcgctgttgc gatggtgaag tctattcgtg ctggtgaagg tgtttcgtac ggacacgact ggattgcgca gcatgacacc aatttggcgc tgttgccggt tggatatgcg gacggtgtgt tccggtcgct gggcggccgg ctggatgtgc tgatcaatgg caagcgccgg cctggcgtcg gacggatctg tatggaccag tttgtcgtcg acttgggtcc ggggccaatc gatgtagccg aaggcgacga agccatcttg ttcggtcccg gcgccagagg cgaacccact gcgcaagatt gggctgatct gcttggaact atccactatg aagtggtcac cagtctacgc gggcggatca ccagaacgta ccgcgaggcc caaaccgttg acagataaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa