MyleA.00185.a

PROBABLE ACYL-CoA DEHYDROGENASE FADE5

CENTER ID: MyleA.00185.a
ORGANISM: Mycobacterium leprae Br4923
ASSOCIATED DISEASE: Hansen's disease, leprosy
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00185.a.A1.GE34160 full length 1 611
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyleA.00185.a.A1.PW34737 full length 1 611

External Resources

RESOURCE REFERENCE ID
OrthoMCL: OG5_136659
PATRIC ID: fig|561304.4.peg.4953
RefSeq: YP_002504269.1
UniProt: A0A0H3N144

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHYKSNVRD QVFNLFEVLG VDKAFGTDEF SNLDADTAYE MLTEVSRLAE GPVAASFAEG DRNPPVFHPE THSITLPESF KKSCRAVAEA GWSKAGIDEA LGGTPMPKAL LWALHEHLMG ANPAVWIYAG GAGFANILYH LGTEKQKKWA VWAAERNWGS TMVLTEPDAG SDVGAGRTKA VHQEDDSWHI DGVKRFITSG DSDDLFENIF HLVLARPEGA GPGTKGLSLF FVPKFLFDFE TGELGERNGV FVTNVEHKMG LKVSATCELS FGQHGVPAKG WLVGDVHDGI AQMFEVIEQA RMMVGTKAIA TLSTGYLNAL EYAKSRVQGA DLTQMTDKTA PRVTITHHPD VRRSLMTQKA YAEGLRALYL YTATFQDAAV AEAVHGVDAK LAVKINDLML PLVKGVGSEQ AYAKLTESLQ TLGGSGFLQD YPIEQYIRDA KIDSLYEGTT AIQAQDFFFR KIVRDKGVAL SYVSGQIQQF VDSETGNGRL KSERELLAKA LTDIQGMEAS LTGYLMAAQQ DVTSLYKVGL GSVRFLMSVG DLIIGWLLQR QAAVAVQALD AGASSAERSF YEGKVAVSSF FAKNFLPLLT STREVLEVLD NDIMELDEAA F
NT Sequence
gtgagtcact acaaaagcaa cgttcgcgac caggttttta acctctttga ggtgttgggc gtggacaagg ctttcggtac agacgagttc agcaatctgg acgccgacac cgcctacgaa atgttgaccg aggtcagccg tctggccgag ggacccgtcg ccgcgtcgtt cgctgaaggc gaccgcaacc caccagtatt ccaccccgaa actcactcga tcacactgcc ggagtcgttc aagaaatcct gcagggcggt ggccgaagcc ggttggagca aggccggcat cgacgaggca ctcggtggca cgccgatgcc caaggcgctg ttatgggcac tgcacgagca tctgatgggc gccaacccgg cggtgtggat ttatgctggc ggtgccggct tcgctaacat cctctaccac ctcggcaccg agaaacagaa gaagtgggcg gtgtgggctg ccgagcgcaa ttggggttcg accatggtgc tcaccgagcc agatgccggt tcggatgtgg gtgccggccg gaccaaggcc gttcatcagg aagacgattc ctggcacatt gatggggtca agcgattcat cacctcgggt gactccgacg acctattcga gaacatcttc cacctcgtgc tggcccgccc cgagggcgcc gggcctggca ccaaggggct gtcactattc ttcgtgccca agttcctgtt cgacttcgaa accggtgagc tcggcgaacg caacggggtc ttcgtcacca acgtcgagca caagatgggc ctcaaggtgt ctgccacttg tgagctgtcc tttggccagc acggcgtgcc cgctaagggc tggctggtcg gcgatgtgca cgatggcata gcgcagatgt ttgaggtcat cgagcaggca cgcatgatgg tcggcaccaa ggcgattgct accctgtcga ccggctatct gaacgcgctg gaatacgcaa agtcccgcgt gcagggcgcc gacctgaccc aaatgaccga caagaccgca ccgcgggtaa ccatcacgca tcaccctgat gtgcgccggt cgctaatgac tcaaaaggcc tacgccgaag gtctgcgagc gctgtacctc tacacagcca cgttccagga tgcagcagtg gccgaagcgg tgcacggcgt ggacgccaag ctggcggtca agatcaacga cctgatgctg ccgttagtca aaggggtggg ctccgagcag gcctacgcga aactcactga gagcctgcag accctcggtg gttcgggctt cctccaagac tacccgattg agcaatacat ccgcgacgcg aaaattgact cgctgtacga aggcaccacc gccatccagg cgcaggactt tttcttccgc aagatcgtcc gcgacaaggg cgtggcactg tcctatgtgt cgggacaaat ccagcaattc gtcgacagcg agactggtaa cggtcggctg aaaagcgaac gcgaactgct ggccaaggcc ctaaccgaca tccaggggat ggaagcctca ctgaccggct atctgatggc agctcagcaa gacgtcacca gcttgtacaa ggtgggtctg gggtcggtgc gcttcctaat gagcgtcggt gacctgatca tcggctggtt gctgcagcgc caggccgcgg tggcggtgca ggcactcgat gccggcgcta gcagcgctga gcgttccttc tacgaaggca aagtcgcggt gtcgtcattc ttcgcgaaaa acttcttgcc actgttgacc agcactcgcg aagtgcttga ggtgctggac aacgacatca tggagcttga cgaagccgcc ttctag
Details for MyleA.00185.a.A1.GE34160
HARVESTED ON: 12/6/2011
SEQUENCED ON: 12/13/2011
EXPECTED MW: 68kDa
OBSERVED MW: 70kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 85
PERCENT COVERAGE: 49
Validated AA Sequence
MLTEVSRLAE GPVAASFAEG DRNPPVFHPE THSITLPESF KKSCRAVAEA GWSKAGIDEA LGGTPMPKAL LWALHEHLMG ANPAVWIYAG GAGFANILYH LGTEKQKKWA VWAAERNWGS TMVLTEPDAG SDVGAGRTKA VHQEDDSWHI DGVKRFITSG DSDDLFENIF HLVLARPEGA GPGTKGLSLF FVPKFLFDFE TGELGERNGV FVTNVEHKMG LKVSATCELS FGQHGVPAXG WLVGXVHDGI AXMFEVIEXA RMMVXXXAXA TXXTGXLXAL XXQSPXXXXX XXXXXXXPXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttnnttn nctttangaa ggagatatac cntggctcat caccatcacc atnnnnnnnn nnnnnnggaa gctcagaccc agggtcctgg ttcgangnnn nnncactaca aaagcaacgt tcgcgaccag gtttttaacc tctttgaggt gttgggcgtg gacaaggctt tcggtacaga cgagttcagc aatctggacg ccgacaccgc ctacgaaatg ttgaccgagg tcagccgtct ggccgaggga cccgtcgccg cgtcgttcgc tgaaggcgac cgcaacccac cagtattcca ccccgaaact cactcgatca cactgccgga gtcgttcaag aaatcctgca gggcggtggc cgaagccggt tggagcaagg ccggcatcga cgaggcactc ggtggcacgc cgatgcccaa ggcgctgtta tgggcactgc acgagcatct gatgggcgcc aacccggcgg tgtggattta tgctggcggt gccggcttcg ctaacatcct ctaccacctc ggcaccgaga aacagaagaa gtgggcggtg tgggctgccg agcgcaattg gggttcgacc atggtgctca ccgagccaga tgccggttcg gatgtgggtg ccggccggac caaggccgtt catcaggaag acgattcctg gcacattgat ggggtcaagc gattcatcac ctcgggtgac tccgacgacc tattcgagaa catcttccac ctcgtgctgg cccgccccga gggcgccggg cctggcacca aggggctgtc actattcttc gtgcccaagt tcctgttcga cttcgaaacc ggtgagctcg gcgaacgcaa cggggtcttc gtcaccaacg tcgagcacaa gatgggcctc aaggtgtctg ccacttgtga gctgtccttt ggccagcacg gcgtgcccgc taanggctgg ctggtcggcn atgtgcacga tggcatagcg canatgtttg aggtcatcga gcnggcacgc atgatggtcn ncannnnngc gatngctacc ntgncgaccg gntntctnna cgcnctnnnn ncgcaaagtc cnnnnnncnn gnnnncnnnc nnacnnnnnn acnnnnnacc nnancnnngn nnnnntnnnn nnnncnnnnn nnnnnnnnnn nnnnnnnnnn nnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSHYKSNV RDQVFNLFEV LGVDKAFGTD EFSNLDADTA YEMLTEVSRL AEGPVAASFA EGDRNPPVFH PETHSITLPE SFKKSCRAVA EAGWSKAGID EALGGTPMPK ALLWALHEHL MGANPAVWIY AGGAGFANIL YHLGTEKQKK WAVWAAERNW GSTMVLTEPD AGSDVGAGRT KAVHQEDDSW HIDGVKRFIT SGDSDDLFEN IFHLVLARPE GAGPGTKGLS LFFVPKFLFD FETGELGERN GVFVTNVEHK MGLKVSATCE LSFGQHGVPA KGWLVGDVHD GIAQMFEVIE QARMMVGTKA IATLSTGYLN ALEYAKSRVQ GADLTQMTDK TAPRVTITHH PDVRRSLMTQ KAYAEGLRAL YLYTATFQDA AVAEAVHGVD AKLAVKINDL MLPLVKGVGS EQAYAKLTES LQTLGGSGFL QDYPIEQYIR DAKIDSLYEG TTAIQAQDFF FRKIVRDKGV ALSYVSGQIQ QFVDSETGNG RLKSERELLA KALTDIQGME ASLTGYLMAA QQDVTSLYKV GLGSVRFLMS VGDLIIGWLL QRQAAVAVQA LDAGASSAER SFYEGKVAVS SFFAKNFLPL LTSTREVLEV LDNDIMELDE AAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtcac tacaaaagca acgttcgcga ccaggttttt aacctctttg aggtgttggg cgtggacaag gctttcggta cagacgagtt cagcaatctg gacgccgaca ccgcctacga aatgttgacc gaggtcagcc gtctggccga gggacccgtc gccgcgtcgt tcgctgaagg cgaccgcaac ccaccagtat tccaccccga aactcactcg atcacactgc cggagtcgtt caagaaatcc tgcagggcgg tggccgaagc cggttggagc aaggccggca tcgacgaggc actcggtggc acgccgatgc ccaaggcgct gttatgggca ctgcacgagc atctgatggg cgccaacccg gcggtgtgga tttatgctgg cggtgccggc ttcgctaaca tcctctacca cctcggcacc gagaaacaga agaagtgggc ggtgtgggct gccgagcgca attggggttc gaccatggtg ctcaccgagc cagatgccgg ttcggatgtg ggtgccggcc ggaccaaggc cgttcatcag gaagacgatt cctggcacat tgatggggtc aagcgattca tcacctcggg tgactccgac gacctattcg agaacatctt ccacctcgtg ctggcccgcc ccgagggcgc cgggcctggc accaaggggc tgtcactatt cttcgtgccc aagttcctgt tcgacttcga aaccggtgag ctcggcgaac gcaacggggt cttcgtcacc aacgtcgagc acaagatggg cctcaaggtg tctgccactt gtgagctgtc ctttggccag cacggcgtgc ccgctaaggg ctggctggtc ggcgatgtgc acgatggcat agcgcagatg tttgaggtca tcgagcaggc acgcatgatg gtcggcacca aggcgattgc taccctgtcg accggctatc tgaacgcgct ggaatacgca aagtcccgcg tgcagggcgc cgacctgacc caaatgaccg acaagaccgc accgcgggta accatcacgc atcaccctga tgtgcgccgg tcgctaatga ctcaaaaggc ctacgccgaa ggtctgcgag cgctgtacct ctacacagcc acgttccagg atgcagcagt ggccgaagcg gtgcacggcg tggacgccaa gctggcggtc aagatcaacg acctgatgct gccgttagtc aaaggggtgg gctccgagca ggcctacgcg aaactcactg agagcctgca gaccctcggt ggttcgggct tcctccaaga ctacccgatt gagcaataca tccgcgacgc gaaaattgac tcgctgtacg aaggcaccac cgccatccag gcgcaggact ttttcttccg caagatcgtc cgcgacaagg gcgtggcact gtcctatgtg tcgggacaaa tccagcaatt cgtcgacagc gagactggta acggtcggct gaaaagcgaa cgcgaactgc tggccaaggc cctaaccgac atccagggga tggaagcctc actgaccggc tatctgatgg cagctcagca agacgtcacc agcttgtaca aggtgggtct ggggtcggtg cgcttcctaa tgagcgtcgg tgacctgatc atcggctggt tgctgcagcg ccaggccgcg gtggcggtgc aggcactcga tgccggcgct agcagcgctg agcgttcctt ctacgaaggc aaagtcgcgg tgtcgtcatt cttcgcgaaa aacttcttgc cactgttgac cagcactcgc gaagtgcttg aggtgctgga caacgacatc atggagcttg acgaagccgc cttctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for MyleA.00185.a.A1.PW34737
PURIFICATION DATe: 1/24/2012
CONCENTRATION: 20.84mg/ml
OBSERVED MW: 70kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 85
PERCENT COVERAGE: 49
Protocol Notes
notes unavailable
Validated AA Sequence
MLTEVSRLAE GPVAASFAEG DRNPPVFHPE THSITLPESF KKSCRAVAEA GWSKAGIDEA LGGTPMPKAL LWALHEHLMG ANPAVWIYAG GAGFANILYH LGTEKQKKWA VWAAERNWGS TMVLTEPDAG SDVGAGRTKA VHQEDDSWHI DGVKRFITSG DSDDLFENIF HLVLARPEGA GPGTKGLSLF FVPKFLFDFE TGELGERNGV FVTNVEHKMG LKVSATCELS FGQHGVPAXG WLVGXVHDGI AXMFEVIEXA RMMVXXXAXA TXXTGXLXAL XXQSPXXXXX XXXXXXXPXX XXXXXXXXXX XXXXX
Validated NT Sequence
attttnnttn nctttangaa ggagatatac cntggctcat caccatcacc atnnnnnnnn nnnnnnggaa gctcagaccc agggtcctgg ttcgangnnn nnncactaca aaagcaacgt tcgcgaccag gtttttaacc tctttgaggt gttgggcgtg gacaaggctt tcggtacaga cgagttcagc aatctggacg ccgacaccgc ctacgaaatg ttgaccgagg tcagccgtct ggccgaggga cccgtcgccg cgtcgttcgc tgaaggcgac cgcaacccac cagtattcca ccccgaaact cactcgatca cactgccgga gtcgttcaag aaatcctgca gggcggtggc cgaagccggt tggagcaagg ccggcatcga cgaggcactc ggtggcacgc cgatgcccaa ggcgctgtta tgggcactgc acgagcatct gatgggcgcc aacccggcgg tgtggattta tgctggcggt gccggcttcg ctaacatcct ctaccacctc ggcaccgaga aacagaagaa gtgggcggtg tgggctgccg agcgcaattg gggttcgacc atggtgctca ccgagccaga tgccggttcg gatgtgggtg ccggccggac caaggccgtt catcaggaag acgattcctg gcacattgat ggggtcaagc gattcatcac ctcgggtgac tccgacgacc tattcgagaa catcttccac ctcgtgctgg cccgccccga gggcgccggg cctggcacca aggggctgtc actattcttc gtgcccaagt tcctgttcga cttcgaaacc ggtgagctcg gcgaacgcaa cggggtcttc gtcaccaacg tcgagcacaa gatgggcctc aaggtgtctg ccacttgtga gctgtccttt ggccagcacg gcgtgcccgc taanggctgg ctggtcggcn atgtgcacga tggcatagcg canatgtttg aggtcatcga gcnggcacgc atgatggtcn ncannnnngc gatngctacc ntgncgaccg gntntctnna cgcnctnnnn ncgcaaagtc cnnnnnncnn gnnnncnnnc nnacnnnnnn acnnnnnacc nnancnnngn nnnnntnnnn nnnncnnnnn nnnnnnnnnn nnnnnnnnnn nnna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSHYKSNV RDQVFNLFEV LGVDKAFGTD EFSNLDADTA YEMLTEVSRL AEGPVAASFA EGDRNPPVFH PETHSITLPE SFKKSCRAVA EAGWSKAGID EALGGTPMPK ALLWALHEHL MGANPAVWIY AGGAGFANIL YHLGTEKQKK WAVWAAERNW GSTMVLTEPD AGSDVGAGRT KAVHQEDDSW HIDGVKRFIT SGDSDDLFEN IFHLVLARPE GAGPGTKGLS LFFVPKFLFD FETGELGERN GVFVTNVEHK MGLKVSATCE LSFGQHGVPA KGWLVGDVHD GIAQMFEVIE QARMMVGTKA IATLSTGYLN ALEYAKSRVQ GADLTQMTDK TAPRVTITHH PDVRRSLMTQ KAYAEGLRAL YLYTATFQDA AVAEAVHGVD AKLAVKINDL MLPLVKGVGS EQAYAKLTES LQTLGGSGFL QDYPIEQYIR DAKIDSLYEG TTAIQAQDFF FRKIVRDKGV ALSYVSGQIQ QFVDSETGNG RLKSERELLA KALTDIQGME ASLTGYLMAA QQDVTSLYKV GLGSVRFLMS VGDLIIGWLL QRQAAVAVQA LDAGASSAER SFYEGKVAVS SFFAKNFLPL LTSTREVLEV LDNDIMELDE AAF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGTGAGTCAC TACAAAAGCA ACGTTCGCGA CCAGGTTTTT AACCTCTTTG AGGTGTTGGG CGTGGACAAG GCTTTCGGTA CAGACGAGTT CAGCAATCTG GACGCCGACA CCGCCTACGA AATGTTGACC GAGGTCAGCC GTCTGGCCGA GGGACCCGTC GCCGCGTCGT TCGCTGAAGG CGACCGCAAC CCACCAGTAT TCCACCCCGA AACTCACTCG ATCACACTGC CGGAGTCGTT CAAGAAATCC TGCAGGGCGG TGGCCGAAGC CGGTTGGAGC AAGGCCGGCA TCGACGAGGC ACTCGGTGGC ACGCCGATGC CCAAGGCGCT GTTATGGGCA CTGCACGAGC ATCTGATGGG CGCCAACCCG GCGGTGTGGA TTTATGCTGG CGGTGCCGGC TTCGCTAACA TCCTCTACCA CCTCGGCACC GAGAAACAGA AGAAGTGGGC GGTGTGGGCT GCCGAGCGCA ATTGGGGTTC GACCATGGTG CTCACCGAGC CAGATGCCGG TTCGGATGTG GGTGCCGGCC GGACCAAGGC CGTTCATCAG GAAGACGATT CCTGGCACAT TGATGGGGTC AAGCGATTCA TCACCTCGGG TGACTCCGAC GACCTATTCG AGAACATCTT CCACCTCGTG CTGGCCCGCC CCGAGGGCGC CGGGCCTGGC ACCAAGGGGC TGTCACTATT CTTCGTGCCC AAGTTCCTGT TCGACTTCGA AACCGGTGAG CTCGGCGAAC GCAACGGGGT CTTCGTCACC AACGTCGAGC ACAAGATGGG CCTCAAGGTG TCTGCCACTT GTGAGCTGTC CTTTGGCCAG CACGGCGTGC CCGCTAAGGG CTGGCTGGTC GGCGATGTGC ACGATGGCAT AGCGCAGATG TTTGAGGTCA TCGAGCAGGC ACGCATGATG GTCGGCACCA AGGCGATTGC TACCCTGTCG ACCGGCTATC TGAACGCGCT GGAATACGCA AAGTCCCGCG TGCAGGGCGC CGACCTGACC CAAATGACCG ACAAGACCGC ACCGCGGGTA ACCATCACGC ATCACCCTGA TGTGCGCCGG TCGCTAATGA CTCAAAAGGC CTACGCCGAA GGTCTGCGAG CGCTGTACCT CTACACAGCC ACGTTCCAGG ATGCAGCAGT GGCCGAAGCG GTGCACGGCG TGGACGCCAA GCTGGCGGTC AAGATCAACG ACCTGATGCT GCCGTTAGTC AAAGGGGTGG GCTCCGAGCA GGCCTACGCG AAACTCACTG AGAGCCTGCA GACCCTCGGT GGTTCGGGCT TCCTCCAAGA CTACCCGATT GAGCAATACA TCCGCGACGC GAAAATTGAC TCGCTGTACG AAGGCACCAC CGCCATCCAG GCGCAGGACT TTTTCTTCCG CAAGATCGTC CGCGACAAGG GCGTGGCACT GTCCTATGTG TCGGGACAAA TCCAGCAATT CGTCGACAGC GAGACTGGTA ACGGTCGGCT GAAAAGCGAA CGCGAACTGC TGGCCAAGGC CCTAACCGAC ATCCAGGGGA TGGAAGCCTC ACTGACCGGC TATCTGATGG CAGCTCAGCA AGACGTCACC AGCTTGTACA AGGTGGGTCT GGGGTCGGTG CGCTTCCTAA TGAGCGTCGG TGACCTGATC ATCGGCTGGT TGCTGCAGCG CCAGGCCGCG GTGGCGGTGC AGGCACTCGA TGCCGGCGCT AGCAGCGCTG AGCGTTCCTT CTACGAAGGC AAAGTCGCGG TGTCGTCATT CTTCGCGAAA AACTTCTTGC CACTGTTGAC CAGCACTCGC GAAGTGCTTG AGGTGCTGGA CAACGACATC ATGGAGCTTG ACGAAGCCGC CTTCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA