SSGCID: Target Page

MyleA.00168.a

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (IspD)

CENTER ID:	MyleA.00168.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00168.a.A2.GE29197	Mature protein( MyleA.00168.a )		22	241

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.513
RefSeq:	YP_002502979.1
UniProt:	B8ZUA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAVATGTVVA VVPAAGAGKR LAAGIPKAFC ELDGRTLVER AVVGLLESGV VDHVVVAVPA DRIAQTQWVL SQRLANSAGQ HATVVAGGAD RTKSVCQALA TLPAPSRVGA PEFILVHDAA RALTPARLIV RVVDALRAGH TAVVPALPLS DTIKAVDANG MVLGTPARVG LRAVQTPQGF ATELLWCAYQ RGPHLDAVDF TDDASLVEHL GGQVQVVAGD PLAFKITTQL DLLLAKKILR R

NT Sequence

ATGGCTGTGG CAACGGGCAC TGTAGTTGCA GTGGTCCCGG CCGCCGGGGC GGGGAAGCGG CTAGCGGCCG GCATTCCGAA GGCATTCTGT GAACTTGACG GGCGTACGCT TGTCGAACGT GCTGTCGTTG GCTTGCTGGA ATCTGGCGTT GTTGACCATG TTGTGGTGGC GGTGCCTGCT GATCGCATTG CCCAGACCCA GTGGGTGCTA TCCCAACGGC TAGCCAATTC GGCGGGTCAA CACGCCACAG TTGTGGCCGG CGGGGCCGAC CGCACCAAAT CGGTGTGTCA GGCTCTTGCG ACCCTTCCCG CCCCGTCAAG GGTGGGCGCA CCCGAGTTTA TATTGGTGCA TGATGCCGCG CGGGCGCTGA CACCAGCACG GTTAATCGTG CGGGTGGTTG ATGCTTTGCG TGCTGGTCAT ACCGCGGTCG TTCCAGCGCT GCCACTGTCT GACACCATTA AAGCCGTGGA CGCCAACGGA ATGGTTCTTG GCACTCCGGC GCGGGTCGGC TTGCGGGCGG TGCAGACCCC GCAGGGGTTT GCTACCGAGC TGCTATGGTG CGCTTATCAG CGTGGCCCCC ATCTTGATGC CGTCGATTTC ACCGACGATG CTTCCCTTGT CGAACATCTC GGTGGCCAGG TTCAGGTGGT CGCCGGCGAT CCACTGGCGT TCAAGATCAC CACTCAGTTG GATCTGCTGC TGGCGAAGAA GATTCTGCGC CGG

Details for MyleA.00168.a.A2.GE29197

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	25kDa
OBSERVED MW:	26kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAAGIPKAF CELDGRTLVE RAVVGLLESG VVDHVVVAVP ADRIAQTQWV LSQRLANSAG QHATVVAGGA DRTKSVCQAL ATLPAPSRVG APEFILVHDA ARALTPARLI VRVVDALRAG HTAVVPALPL SDTIKAVDAN GMVLGTPARV GLRAVQTPQG FATELLWCAY QRGPHLDAVD FTDDASLVEH LGGQVQVVAG DPLAFKITTQ LDLLLAKKIL RR

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg ccggcattcc gaaggcattc tgtgaacttg acgggcgtac gcttgtcgaa cgtgctgtcg ttggcttgct ggaatctggc gttgttgacc atgttgtggt ggcggtgcct gctgatcgca ttgcccagac ccagtgggtg ctatcccaac ggctagccaa ttcggcgggt caacacgcca cagttgtggc cggcggggcc gaccgcacca aatcggtgtg tcaggctctt gcgacccttc ccgccccgtc aagggtgggc gcacccgagt ttatattggt gcatgatgcc gcgcgggcgc tgacaccagc acggttaatc gtgcgggtgg ttgatgcttt gcgtgctggt cataccgcgg tcgttccagc gctgccactg tctgacacca ttaaagccgt ggacgccaac ggaatggttc ttggcactcc ggcgcgggtc ggcttgcggg cggtgcagac cccgcagggg tttgctaccg agctgctatg gtgcgcttat cagcgtggcc cccatcttga tgccgtcgat ttcaccgacg atgcttccct tgtcgaacat ctcggtggcc aggttcaggt ggtcgccggc gatccactgg cgttcaagat caccactcag ttggatctgc tgctggcgaa gaagattctg cgccggtaat aa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggccggc attccgaagg cattctgtga acttgacggg cgtacgcttg tcgaacgtgc tgtcgttggc ttgctggaat ctggcgttgt tgaccatgtt gtggtggcgg tgcctgctga tcgcattgcc cagacccagt gggtgctatc ccaacggcta gccaattcgg cgggtcaaca cgccacagtt gtggccggcg gggccgaccg caccaaatcg gtgtgtcagg ctcttgcgac ccttcccgcc ccgtcaaggg tgggcgcacc cgagtttata ttggtgcatg atgccgcgcg ggcgctgaca ccagcacggt taatcgtgcg ggtggttgat gctttgcgtg ctggtcatac cgcggtcgtt ccagcgctgc cactgtctga caccattaaa gccgtggacg ccaacggaat ggttcttggc actccggcgc gggtcggctt gcgggcggtg cagaccccgc aggggtttgc taccgagctg ctatggtgcg cttatcagcg tggcccccat cttgatgccg tcgatttcac cgacgatgct tcccttgtcg aacatctcgg tggccaggtt caggtggtcg ccggcgatcc actggcgttc aagatcacca ctcagttgga tctgctgctg gcgaagaaga ttctgcgccg gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa