SSGCID: Target Page

MyleA.00161.a

PROBABLE ADENOSINE DEAMINASE ADD (ADENOSINE AMINOHYDROLASE)

CENTER ID:	MyleA.00161.a
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00161.a.A1.GE34143	full length		1	362

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.1261
RefSeq:	YP_002503190.1
UniProt:	B8ZUW8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTPLHLENI KQAPKALLHD HLDGGLRPAT VLDIAGQVGY DRLPATDVES LETWFRTASH SGSLERYLEP FSHTVAVMQT PEALHRVAYE CVEDLAADSV VYAEVRFAPE LHIDEGLSFD EVLASVLAGF ADGERACAAE GNAITVRCLV TAMRHAAMSR EIAELAIRFR DKGVVGFDIA GAEAGHPPTR HLDAFEYMRS NNARFTIHAG EAFGLPSIHE AIAFCGADRL GHGVRIVDDI DVDPGGGIRL GPLASILRDK RIPLELCPSS NLQTGAVASI TEHPFDLLAW ARFRVTVNTD NRLMSDTSMS LEMHRLVEAF GYGWGDLERF TINAMKSAFI PFDQRLAIID EVIKPRFAVL VG

NT Sequence

atgaatacgc cattgcacct agaaaacatc aagcaagcgc cgaaggccct gctgcatgac caccttgacg gtgggctgcg cccagcgacc gtgttggata ttgccggcca ggtcggctat gataggctcc ccgctaccga cgttgagtcg ctagagacat ggtttcgcac tgcttcgcac agcggctcgt tggagcgcta cctggagccg ttttctcata ccgtggccgt gatgcagaca ccagaggcgt tgcaccgcgt cgcttatgag tgcgtggaag acctcgccgc tgattcggtg gtctacgccg aagtccggtt cgctccggag ctgcacatcg acgaggggtt gtcgtttgat gaggtcctcg caagcgtgct ggcgggtttt gctgacggtg aaagggcttg tgccgccgag ggcaatgcca tcacagtgcg ctgcttggtc acagcgatgc ggcacgcggc aatgtcgcgg gagatcgccg agctagcgat ccggttccgg gacaaaggag tcgtcggatt cgacatcgct ggtgctgagg ctgggcaccc tccgactcgg cacctcgacg cttttgagta catgcgtagt aataatgcgc gcttcactat tcatgcgggt gaggcgttcg ggctgccatc tatccacgag gcgatcgcgt tctgcggagc tgaccggctg ggccacgggg tgcggatcgt cgacgacatc gacgttgatc ctggtggtgg catccggttg ggtcccctgg cctcgatcct gcgggacaag cgaataccgc tggagctgtg ccccagctcg aacctgcaaa ccggcgcggt ggcaagcatc accgagcacc cgtttgatct gctggcctgg gcacgtttcc gggtgacagt caacaccgac aaccggctta tgagcgacac gtcgatgagc ctggagatgc accgcttggt ggaggcgttc ggctacggtt ggggtgatct cgaacgtttc accatcaatg ctatgaagtc ggcgtttatt ccgttcgatc aacggttagc aataatcgat gaggttatca agcctcggtt cgccgtgctg gtcggatag

Details for MyleA.00161.a.A1.GE34143

HARVESTED ON:	12/6/2011
SEQUENCED ON:	12/8/2011
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	77
PERCENT COVERAGE:	104

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMNTPLHLEN IKQAPKALLH DHLDGGLRPA TVLDIAGQVG YDRLPATDVE SLETWFRTAS HSGSLERYLE PFSHTVAVMQ TPEALHRVAY ECVEDLAADS VVYAEVRFAP ELHIDEGLSF DEVLTSVLAG FADGERACAA EGNAITVRCL VTAMRHAAMS REIAELAIRF RDKGVVGFDI AGAEAGHPPT RHLDAFEYMR SNNARFTIHA GEAFGLPSIH EAIAFCGADR LGHGVRIVDD IDVDPGGGIR LGPLASILRD KRIPLELCPS SNLXTGAXQA SXSTRXIXWP GHVXXTVXXX XXXXXSXXXX XXXXXXXXXG XXXXXSXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXX

Validated NT Sequence

tttntttact ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaatacg ccattgcacc tagaaaacat caagcaagcg ccgaaggccc tgctgcatga ccaccttgac ggtgggctgc gcccagcgac cgtgttggat attgccggcc aggtcggcta tgataggctc cccgctaccg acgttgagtc gctagagaca tggtttcgca ctgcttcgca cagcggctcg ttggagcgct acctggagcc gttttctcat accgtggccg tgatgcagac accagaggcg ttgcaccgcg tcgcttatga gtgcgtggaa gacctcgccg ctgattcggt ggtctacgcc gaagtccggt tcgctccgga gctgcacatc gacgaggggt tgtcgtttga tgaggtcctc acaagcgtgc tggcgggttt tgctgacggt gaaagggctt gtgccgccga gggcaatgcc atcacagtgc gctgcttggt cacagcgatg cggcacgcgg caatgtcgcg ggagatcgcc gagctagcga tccggttccg ggacaaagga gtcgtcggat tcgacatcgc tggtgctgag gctgggcacc ctccgactcg gcacctcgac gcttttgagt acatgcgtag taataatgcg cgcttcacta ttcatgcggg tgaggcgttc gggctgccat ctatccacga ggcgatcgcg ttctgcggag ctgaccggct gggccacggg gtgcggatcg tcgacgacat cgacgttgat cctggtggtg gcatccggtt gggtcccctg gcctcgatcc tgcgggacaa gcgaataccg ctggagctgt gccccagctc gaacctgnaa accggcgcgn ggcaagcatc ancgagcacc cgtttnatct gntggcctgg gcacgtttnn ntgacagtca nncnannnnn tnngancnan nngtcnatna ncnnnnnnnn nnccnntgng nnnnntnnnt annnnggnng nnnnncnaan gnttcncnnn nnnggnannc nnnnnttntn cntnnnannn nnnnngnana ncnannnnnn ntnancnnnn nnnnnnnnnn ngnnnnnnnn annnnnnnna nanntnnnnn nnntnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaannn nannnnac

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNTPLHLEN IKQAPKALLH DHLDGGLRPA TVLDIAGQVG YDRLPATDVE SLETWFRTAS HSGSLERYLE PFSHTVAVMQ TPEALHRVAY ECVEDLAADS VVYAEVRFAP ELHIDEGLSF DEVLASVLAG FADGERACAA EGNAITVRCL VTAMRHAAMS REIAELAIRF RDKGVVGFDI AGAEAGHPPT RHLDAFEYMR SNNARFTIHA GEAFGLPSIH EAIAFCGADR LGHGVRIVDD IDVDPGGGIR LGPLASILRD KRIPLELCPS SNLQTGAVAS ITEHPFDLLA WARFRVTVNT DNRLMSDTSM SLEMHRLVEA FGYGWGDLER FTINAMKSAF IPFDQRLAII DEVIKPRFAV LVG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaatacgcca ttgcacctag aaaacatcaa gcaagcgccg aaggccctgc tgcatgacca ccttgacggt gggctgcgcc cagcgaccgt gttggatatt gccggccagg tcggctatga taggctcccc gctaccgacg ttgagtcgct agagacatgg tttcgcactg cttcgcacag cggctcgttg gagcgctacc tggagccgtt ttctcatacc gtggccgtga tgcagacacc agaggcgttg caccgcgtcg cttatgagtg cgtggaagac ctcgccgctg attcggtggt ctacgccgaa gtccggttcg ctccggagct gcacatcgac gaggggttgt cgtttgatga ggtcctcgca agcgtgctgg cgggttttgc tgacggtgaa agggcttgtg ccgccgaggg caatgccatc acagtgcgct gcttggtcac agcgatgcgg cacgcggcaa tgtcgcggga gatcgccgag ctagcgatcc ggttccggga caaaggagtc gtcggattcg acatcgctgg tgctgaggct gggcaccctc cgactcggca cctcgacgct tttgagtaca tgcgtagtaa taatgcgcgc ttcactattc atgcgggtga ggcgttcggg ctgccatcta tccacgaggc gatcgcgttc tgcggagctg accggctggg ccacggggtg cggatcgtcg acgacatcga cgttgatcct ggtggtggca tccggttggg tcccctggcc tcgatcctgc gggacaagcg aataccgctg gagctgtgcc ccagctcgaa cctgcaaacc ggcgcggtgg caagcatcac cgagcacccg tttgatctgc tggcctgggc acgtttccgg gtgacagtca acaccgacaa ccggcttatg agcgacacgt cgatgagcct ggagatgcac cgcttggtgg aggcgttcgg ctacggttgg ggtgatctcg aacgtttcac catcaatgct atgaagtcgg cgtttattcc gttcgatcaa cggttagcaa taatcgatga ggttatcaag cctcggttcg ccgtgctggt cggataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa