SSGCID: Target Page

MyleA.00148.b

Integrase/recombinase

CENTER ID:	MyleA.00148.b
ORGANISM:	Mycobacterium leprae Br4923
ASSOCIATED DISEASE:	Hansen's disease, leprosy
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyleA.00148.b.A1.GE29194	Full length( MyleA.00148.b )		1	297

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|561304.4.peg.3022
RefSeq:	YP_002503732.1
UniProt:	A0A0H3MR74

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAILDEFDEH LALRCGRSEH TRRAYLGDLR SLLTHLEGRG SRLDQLSLPV LRSWLATAAG AGAARTTLAR RISAVKAFTA WAKWRGQLAA DPAARLQVPR AYRTLPAVLR QDQALQIMAA AKSGAEQGDP LALRDRLIVE MLYATGIRVS ELCGLDIDDV DTRHRLVRVL GKGNKQRTAP FGVPAADALR GWLDDGRPAL VTVESGPALL LGSRGRRLDV RQARTVVHQT VAVVAGAPDM GPHGLRHSAA THLLEGGADL RVVQELLGHS SLATTQLYTH VAVSRLRVVH DQAHPRA

NT Sequence

GTGGCGATCC TCGACGAGTT CGATGAGCAC CTAGCGCTGC GGTGCGGTCG CTCAGAGCAT ACCCGTCGTG CATATCTGGG CGACCTGCGG TCGTTGTTGA CCCATCTAGA GGGACGTGGG TCCAGGCTAG ATCAACTGAG CCTGCCGGTG CTGCGGTCGT GGCTGGCTAC GGCGGCCGGT GCCGGCGCGG CTCGCACCAC GTTGGCCCGT CGCATCTCGG CGGTCAAGGC ATTCACCGCG TGGGCGAAGT GGCGTGGCCA ACTTGCGGCC GACCCCGCCG CGCGGTTACA GGTGCCTAGG GCGTATCGCA CCCTGCCGGC GGTGCTGCGT CAGGATCAGG CGTTGCAGAT CATGGCGGCC GCAAAATCCG GCGCCGAGCA GGGTGATCCT CTGGCTTTGC GGGACCGGTT GATCGTAGAA ATGTTGTACG CCACCGGCAT CCGGGTGAGT GAGTTGTGCG GCTTGGACAT CGACGATGTG GACACCCGGC ACCGGCTGGT GCGTGTCCTG GGTAAGGGCA ATAAACAGCG CACCGCGCCG TTTGGGGTAC CGGCCGCTGA CGCGCTGCGC GGCTGGCTGG ACGACGGGCG CCCGGCTTTG GTCACCGTAG AGTCCGGGCC AGCACTACTC CTGGGATCAC GGGGGCGACG GCTCGACGTG CGCCAGGCCC GCACGGTGGT GCACCAAACC GTGGCCGTGG TAGCCGGTGC ACCCGACATG GGACCGCATG GGCTGCGGCA CAGCGCAGCC ACTCATTTGC TTGAAGGCGG TGCTGACCTG CGTGTCGTCC AGGAATTGCT AGGGCATTCC AGTCTGGCGA CCACCCAACT GTACACCCAC GTGGCGGTCT CACGGCTGCG TGTGGTGCAC GACCAGGCTC ACCCGCGGGC G

Details for MyleA.00148.b.A1.GE29194

HARVESTED ON:	4/13/2010
SEQUENCED ON:	4/24/2010
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	93

Validated AA Sequence

MVAILDEFDE HLALRCGRSE HTRRAYLGDL RSLLTHLEGR GSRLDQLSLP VLRSWLATAA GAGAARTTLA RRISAVKAFT AWAKWRGQLA ADPAARLQVP RAYRTLPAVL RQDQALQIMA AAKSGAEQGD PLALRDRLIV EMLYATGIRV SELCGLDIDD VDTRHRLVRV LGKGNKQRTA PFGVPAADAL RGWLDDGRPA LVTVESGPAL LLGSRGRRLD VRQARTVVHQ TVAVVAGAPD MGPHGLRHSA ATHLLEGGAD LRVVQELLGH SSLATTQLYT HVAVXRLRVV HDQAHPR

Validated NT Sequence

ggaagctcag acccagggtc ctggttcgat ggtggcgatc ctcgacgagt tcgatgagca cctagcgctg cggtgcggtc gctcagagca tacccgtcgt gcatatctgg gcgacctgcg gtcgttgttg acccatctag agggacgtgg gtccaggcta gatcaactga gcctgccggt gctgcggtcg tggctggcta cggcggccgg tgccggcgcg gctcgcacca cgttggcccg tcgcatctcg gcggtcaagg cattcaccgc gtgggcgaag tggcgtggcc aacttgcggc cgaccccgcc gcgcggttac aggtgcctag ggcgtatcgc accctgccgg cggtgctgcg tcaggatcag gcgttgcaga tcatggcggc cgcaaaatcc ggcgccgagc agggtgatcc tctggctttg cgggaccggt tgatcgtaga aatgttgtac gccaccggca tccgggtgag tgagttgtgc ggcttggaca tcgacgatgt ggacacccgg caccggctgg tgcgtgtcct gggtaagggc aataaacagc gcaccgcgcc gtttggggta ccggccgctg acgcgctgcg cggctggctg gacgacgggc gcccggcttt ggtcaccgta gagtccgggc cagcactact cctgggatca cgggggcgac ggctcgacgt gcgccaggcc cgcacggtgg tgcaccaaac cgtggccgtg gtagccggtg cacccgacat gggaccgcat gggctgcggc acagcgcagc cactcatttg cttgaaggcg gtgctgacct gcgtgtcgtc caggaattgc tagggcattc cagtctggcg accacccaac tgtacaccca cgtggcggtc ncacggctgc gtgtggtgca cgaccaggct cacccgcggg c

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMVAILDEFD EHLALRCGRS EHTRRAYLGD LRSLLTHLEG RGSRLDQLSL PVLRSWLATA AGAGAARTTL ARRISAVKAF TAWAKWRGQL AADPAARLQV PRAYRTLPAV LRQDQALQIM AAAKSGAEQG DPLALRDRLI VEMLYATGIR VSELCGLDID DVDTRHRLVR VLGKGNKQRT APFGVPAADA LRGWLDDGRP ALVTVESGPA LLLGSRGRRL DVRQARTVVH QTVAVVAGAP DMGPHGLRHS AATHLLEGGA DLRVVQELLG HSSLATTQLY THVAVSRLRV VHDQAHPRA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtggcgatc ctcgacgagt tcgatgagca cctagcgctg cggtgcggtc gctcagagca tacccgtcgt gcatatctgg gcgacctgcg gtcgttgttg acccatctag agggacgtgg gtccaggcta gatcaactga gcctgccggt gctgcggtcg tggctggcta cggcggccgg tgccggcgcg gctcgcacca cgttggcccg tcgcatctcg gcggtcaagg cattcaccgc gtgggcgaag tggcgtggcc aacttgcggc cgaccccgcc gcgcggttac aggtgcctag ggcgtatcgc accctgccgg cggtgctgcg tcaggatcag gcgttgcaga tcatggcggc cgcaaaatcc ggcgccgagc agggtgatcc tctggctttg cgggaccggt tgatcgtaga aatgttgtac gccaccggca tccgggtgag tgagttgtgc ggcttggaca tcgacgatgt ggacacccgg caccggctgg tgcgtgtcct gggtaagggc aataaacagc gcaccgcgcc gtttggggta ccggccgctg acgcgctgcg cggctggctg gacgacgggc gcccggcttt ggtcaccgta gagtccgggc cagcactact cctgggatca cgggggcgac ggctcgacgt gcgccaggcc cgcacggtgg tgcaccaaac cgtggccgtg gtagccggtg cacccgacat gggaccgcat gggctgcggc acagcgcagc cactcatttg cttgaaggcg gtgctgacct gcgtgtcgtc caggaattgc tagggcattc cagtctggcg accacccaac tgtacaccca cgtggcggtc tcacggctgc gtgtggtgca cgaccaggct cacccgcggg cgaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa